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The map label for this gene is ispD

Identifier: 50085134

GI number: 50085134

Start: 1990312

End: 1991031

Strand: Reverse

Name: ispD

Synonym: ACIAD1999

Alternate gene names: 50085134

Gene position: 1991031-1990312 (Counterclockwise)

Preceding gene: 50085135

Following gene: 161349963

Centisome position: 55.33

GC content: 39.58

Gene sequence:

>720_bases
ATGAAACCCCACCTTCACCAGATATCCCAGATTAAATTATGGGCAATTATTCCTGCTGCTGGCTCTGGGAGTCGTTTCTC
TAAAACAGCGTTAAAACAATATCAAACTATTGAAAACAAGACTGTGCTCGAACATACAGTTGAGCGATTAAATGGTCTAA
CACTTGAAGGGTATGTTCTCGCAATTAACGAGCATGATCATGTTGCCCAGAATTTGCCTCTTACCCAGAAGCATAAAGCA
CATTTTTGCAAGGGTGGATTAGAGAGGGTTGATTCTGTACTTAACGCTTTACAATATTTGTCGTCCATCGCATCAGATGA
AGATTATGTGCTGGTACATGATGCTGCTCGACCATGTGTGAGTCTTTCTTGCCTGCAAGCTTTGGCTGAATATGCGATCA
AATATACAACGAATGCAATTCTGGCCATTCCTGTAAGAGATACTCTTAAACAGGTCGTTGATCGTTCGCATATCTCTAAA
ACCATTGATAGAAATCATTTGTGGCAAGCTCAAACTCCACAAATCGCAAAAATTGGTGTGCTTAAACATGCTATTCAATT
GGCTTTAGAAAATAATATTAAGATCACAGATGAAGCAAGTGCGCTGGAATATGCTGGTGCAGTGGTTGATGTGGTTCAAG
GCCGTTCCGATAATATTAAAATTACCTATCAAGATGACCTCGAATTAGCTCGCCTGATTTTATTATCTCAAAAATCTTAG

Upstream 100 bases:

>100_bases
TTAATTAAACCTCATGAAACCTTTGTGCAAATGAGTACCATCAGTACTCATTATAAGCCAATTTATATCGATCCAAATGC
AAAAGTAGACACTCAAACTA

Downstream 100 bases:

>100_bases
ATGAAAACTTAATATGGCAATGTTTAAAAATTTTATAAGTTAATTAGAGTTATAAGATTTTATCCTACACATAAAACTAC
AAACCAATCACGATAAAAAA

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MKPHLHQISQIKLWAIIPAAGSGSRFSKTALKQYQTIENKTVLEHTVERLNGLTLEGYVLAINEHDHVAQNLPLTQKHKA
HFCKGGLERVDSVLNALQYLSSIASDEDYVLVHDAARPCVSLSCLQALAEYAIKYTTNAILAIPVRDTLKQVVDRSHISK
TIDRNHLWQAQTPQIAKIGVLKHAIQLALENNIKITDEASALEYAGAVVDVVQGRSDNIKITYQDDLELARLILLSQKS

Sequences:

>Translated_239_residues
MKPHLHQISQIKLWAIIPAAGSGSRFSKTALKQYQTIENKTVLEHTVERLNGLTLEGYVLAINEHDHVAQNLPLTQKHKA
HFCKGGLERVDSVLNALQYLSSIASDEDYVLVHDAARPCVSLSCLQALAEYAIKYTTNAILAIPVRDTLKQVVDRSHISK
TIDRNHLWQAQTPQIAKIGVLKHAIQLALENNIKITDEASALEYAGAVVDVVQGRSDNIKITYQDDLELARLILLSQKS
>Mature_239_residues
MKPHLHQISQIKLWAIIPAAGSGSRFSKTALKQYQTIENKTVLEHTVERLNGLTLEGYVLAINEHDHVAQNLPLTQKHKA
HFCKGGLERVDSVLNALQYLSSIASDEDYVLVHDAARPCVSLSCLQALAEYAIKYTTNAILAIPVRDTLKQVVDRSHISK
TIDRNHLWQAQTPQIAKIGVLKHAIQLALENNIKITDEASALEYAGAVVDVVQGRSDNIKITYQDDLELARLILLSQKS

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP)

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family

Homologues:

Organism=Escherichia coli, GI1789104, Length=228, Percent_Identity=42.1052631578947, Blast_Score=157, Evalue=4e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPD_ACIAD (Q6FAU1)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_046644.1
- ProteinModelPortal:   Q6FAU1
- SMR:   Q6FAU1
- STRING:   Q6FAU1
- GeneID:   2879843
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD1999
- NMPDR:   fig|62977.3.peg.1906
- eggNOG:   COG1211
- HOGENOM:   HBG672839
- OMA:   FPQGAIL
- PhylomeDB:   Q6FAU1
- ProtClustDB:   PRK00155
- BioCyc:   ASP62977:ACIAD1999-MONOMER
- HAMAP:   MF_00108
- InterPro:   IPR001228
- InterPro:   IPR018294
- TIGRFAMs:   TIGR00453

Pfam domain/function: PF01128 IspD

EC number: =2.7.7.60

Molecular weight: Translated: 26557; Mature: 26557

Theoretical pI: Translated: 7.74; Mature: 7.74

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPHLHQISQIKLWAIIPAAGSGSRFSKTALKQYQTIENKTVLEHTVERLNGLTLEGYVL
CCCCHHHHHHEEEEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEEEE
AINEHDHVAQNLPLTQKHKAHFCKGGLERVDSVLNALQYLSSIASDEDYVLVHDAARPCV
EECCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHH
SLSCLQALAEYAIKYTTNAILAIPVRDTLKQVVDRSHISKTIDRNHLWQAQTPQIAKIGV
HHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
LKHAIQLALENNIKITDEASALEYAGAVVDVVQGRSDNIKITYQDDLELARLILLSQKS
HHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHCCCC
>Mature Secondary Structure
MKPHLHQISQIKLWAIIPAAGSGSRFSKTALKQYQTIENKTVLEHTVERLNGLTLEGYVL
CCCCHHHHHHEEEEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEEEE
AINEHDHVAQNLPLTQKHKAHFCKGGLERVDSVLNALQYLSSIASDEDYVLVHDAARPCV
EECCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHH
SLSCLQALAEYAIKYTTNAILAIPVRDTLKQVVDRSHISKTIDRNHLWQAQTPQIAKIGV
HHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
LKHAIQLALENNIKITDEASALEYAGAVVDVVQGRSDNIKITYQDDLELARLILLSQKS
HHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA