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The map label for this gene is dadA [H]

Identifier: 161349963

GI number: 161349963

Start: 1988686

End: 1989930

Strand: Reverse

Name: dadA [H]

Synonym: ACIAD1998

Alternate gene names: 161349963

Gene position: 1989930-1988686 (Counterclockwise)

Preceding gene: 50085134

Following gene: 50085131

Centisome position: 55.3

GC content: 39.68

Gene sequence:

>1245_bases
ATGTCTCATATTATTGTTATTGGCGCAGGAATTACGGGTGTAACCTCTGCGTATGAACTGATAAAACTCGGGTTTCAGGT
GACTGTTATTGATCGCCATTTATTTCCAGCGATGGAAACATCTTTTGCGAATGGTGGACAGCTTTCAGCTTGTAATGCAG
AAGTATGGAATCAGAGGTCGACAATATCCAAAGGAATTAAATGGATGTCGAAGAAAGATGCACCTCTATTGCTCAATCCT
TCATTTAATCTACACAAATATAGCTGGCTATTTGAATTTTTATCGCATATTAAACATTACGAACAAAATACAATAGAGAC
TGTGCGTCTTGCATTACTTGCAAGAAAACGGCTATTTGAAATTGCAGAAATTGAAAATATCGATTTTAATCTGGAAAGAA
GGGGAATTTTACATATTTATCACACCCCAAGTGATTATGAAATTGCAAAAAATGTGAATGACCTTTTAAACAAAGCCACA
TTGCATCGTTATCAGGTTAATCCTGACGAAATAAAAACAATAGAACCAGCATTATCTGGTGAATACTGTGGGGGGTATTA
CACACCTGATGATGCGACTGGTGATATTCATAAATTTTGTGTGGGGCTGGCAGATTCTTCCAAGCGGGATGGTGTAGATT
ATAAATTCGGCTGGGATGTAAATCAGATAGAGCTTTTGGAAAAAGGCGTGATTGTATCGTGTATTCCGAGTACAGAAAAT
ATGACTGAGCCATCGAGTCAACACCTCAGGCTAGAAGCAGATGGGATAGTGGTTTGTGGTGGTGTCGGAAGTTATCAACT
TGCACATATGTTGGGTGATCGCGTGAATGTGTATCCTGTAAAAGGCTACTCCATTACAGTTCATCTTAATGATGAACACA
GCATCGAAAATGCGCCATGGGTAAGTTTATTAGATGAAAGCGCGAAAATTGTAACCTCTCGTTTAGGTAAAGACCGATTA
CGTGTAGCAGGAACTGCTGAATTTAATGGTTATAATCGCGATATACGTGCCGATAGGATTCAACCACTAATAAACTGGGT
AAATGCAAATTTTGAGATCTCTACAGAGCACGTGGTACCGTGGTCTGGGTTAAGACCGATGATGCCCAATATGATGCCTG
TTGTTAGACAAGGTAAGCATCCACGTGTTTTTTATAATACAGGGCATGGTCATCTGGGGTGGACTCTTTCGGCTGCTACA
GCTGCAATTATTGGTCAGGAAATCCAGTCAAAAATACCAGTCTAA

Upstream 100 bases:

>100_bases
TGGTACGTAGATTTATGGAAATCTATAGCTACCGAAGCTCTCAGGTACCCTGACAGATGGGGCAACTTCTGAAATGTTTA
TACATTTCAAGCGAGTAGAT

Downstream 100 bases:

>100_bases
AACTTTAAGAAACGGCATATTTGTCAGAATATGCCGTTTAATCATATAAGCGATATATTCCCGAAAAGCGTTCACATCCT
TTTTGCTGTGTTTTTACAAC

Product: D-amino acid dehydrogenase small subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 414; Mature: 413

Protein sequence:

>414_residues
MSHIIVIGAGITGVTSAYELIKLGFQVTVIDRHLFPAMETSFANGGQLSACNAEVWNQRSTISKGIKWMSKKDAPLLLNP
SFNLHKYSWLFEFLSHIKHYEQNTIETVRLALLARKRLFEIAEIENIDFNLERRGILHIYHTPSDYEIAKNVNDLLNKAT
LHRYQVNPDEIKTIEPALSGEYCGGYYTPDDATGDIHKFCVGLADSSKRDGVDYKFGWDVNQIELLEKGVIVSCIPSTEN
MTEPSSQHLRLEADGIVVCGGVGSYQLAHMLGDRVNVYPVKGYSITVHLNDEHSIENAPWVSLLDESAKIVTSRLGKDRL
RVAGTAEFNGYNRDIRADRIQPLINWVNANFEISTEHVVPWSGLRPMMPNMMPVVRQGKHPRVFYNTGHGHLGWTLSAAT
AAIIGQEIQSKIPV

Sequences:

>Translated_414_residues
MSHIIVIGAGITGVTSAYELIKLGFQVTVIDRHLFPAMETSFANGGQLSACNAEVWNQRSTISKGIKWMSKKDAPLLLNP
SFNLHKYSWLFEFLSHIKHYEQNTIETVRLALLARKRLFEIAEIENIDFNLERRGILHIYHTPSDYEIAKNVNDLLNKAT
LHRYQVNPDEIKTIEPALSGEYCGGYYTPDDATGDIHKFCVGLADSSKRDGVDYKFGWDVNQIELLEKGVIVSCIPSTEN
MTEPSSQHLRLEADGIVVCGGVGSYQLAHMLGDRVNVYPVKGYSITVHLNDEHSIENAPWVSLLDESAKIVTSRLGKDRL
RVAGTAEFNGYNRDIRADRIQPLINWVNANFEISTEHVVPWSGLRPMMPNMMPVVRQGKHPRVFYNTGHGHLGWTLSAAT
AAIIGQEIQSKIPV
>Mature_413_residues
SHIIVIGAGITGVTSAYELIKLGFQVTVIDRHLFPAMETSFANGGQLSACNAEVWNQRSTISKGIKWMSKKDAPLLLNPS
FNLHKYSWLFEFLSHIKHYEQNTIETVRLALLARKRLFEIAEIENIDFNLERRGILHIYHTPSDYEIAKNVNDLLNKATL
HRYQVNPDEIKTIEPALSGEYCGGYYTPDDATGDIHKFCVGLADSSKRDGVDYKFGWDVNQIELLEKGVIVSCIPSTENM
TEPSSQHLRLEADGIVVCGGVGSYQLAHMLGDRVNVYPVKGYSITVHLNDEHSIENAPWVSLLDESAKIVTSRLGKDRLR
VAGTAEFNGYNRDIRADRIQPLINWVNANFEISTEHVVPWSGLRPMMPNMMPVVRQGKHPRVFYNTGHGHLGWTLSAATA
AIIGQEIQSKIPV

Specific function: Oxidative deamination of D-amino acids [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Inner Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DadA oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787438, Length=420, Percent_Identity=31.6666666666667, Blast_Score=201, Evalue=8e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023080
- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: =1.4.99.1 [H]

Molecular weight: Translated: 46411; Mature: 46280

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHIIVIGAGITGVTSAYELIKLGFQVTVIDRHLFPAMETSFANGGQLSACNAEVWNQRS
CCEEEEEECCCHHHHHHHHHHHCCCEEEEEECHHHHHHHHCCCCCCCEEECCHHHHCCHH
TISKGIKWMSKKDAPLLLNPSFNLHKYSWLFEFLSHIKHYEQNTIETVRLALLARKRLFE
HHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAEIENIDFNLERRGILHIYHTPSDYEIAKNVNDLLNKATLHRYQVNPDEIKTIEPALSG
HHHHCCCCCEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHEEEECCHHHHEEECCCCCC
EYCGGYYTPDDATGDIHKFCVGLADSSKRDGVDYKFGWDVNQIELLEKGVIVSCIPSTEN
CCCCCEECCCCCCCHHHHHHHHCCCCCCCCCCCEEECCCCHHHHHHHCCEEEEEECCCCC
MTEPSSQHLRLEADGIVVCGGVGSYQLAHMLGDRVNVYPVKGYSITVHLNDEHSIENAPW
CCCCCCCCEEEEECCEEEECCCCHHHHHHHHCCCEEEEEECCEEEEEEECCCCCCCCCCH
VSLLDESAKIVTSRLGKDRLRVAGTAEFNGYNRDIRADRIQPLINWVNANFEISTEHVVP
HHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHCCCEEEECCEECC
WSGLRPMMPNMMPVVRQGKHPRVFYNTGHGHLGWTLSAATAAIIGQEIQSKIPV
CCCCCCCCCCHHHHHHCCCCCEEEEECCCCCCCEEHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SHIIVIGAGITGVTSAYELIKLGFQVTVIDRHLFPAMETSFANGGQLSACNAEVWNQRS
CEEEEEECCCHHHHHHHHHHHCCCEEEEEECHHHHHHHHCCCCCCCEEECCHHHHCCHH
TISKGIKWMSKKDAPLLLNPSFNLHKYSWLFEFLSHIKHYEQNTIETVRLALLARKRLFE
HHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAEIENIDFNLERRGILHIYHTPSDYEIAKNVNDLLNKATLHRYQVNPDEIKTIEPALSG
HHHHCCCCCEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHEEEECCHHHHEEECCCCCC
EYCGGYYTPDDATGDIHKFCVGLADSSKRDGVDYKFGWDVNQIELLEKGVIVSCIPSTEN
CCCCCEECCCCCCCHHHHHHHHCCCCCCCCCCCEEECCCCHHHHHHHCCEEEEEECCCCC
MTEPSSQHLRLEADGIVVCGGVGSYQLAHMLGDRVNVYPVKGYSITVHLNDEHSIENAPW
CCCCCCCCEEEEECCEEEECCCCHHHHHHHHCCCEEEEEECCEEEEEEECCCCCCCCCCH
VSLLDESAKIVTSRLGKDRLRVAGTAEFNGYNRDIRADRIQPLINWVNANFEISTEHVVP
HHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHCCCEEEECCEECC
WSGLRPMMPNMMPVVRQGKHPRVFYNTGHGHLGWTLSAATAAIIGQEIQSKIPV
CCCCCCCCCCHHHHHHCCCCCEEEEECCCCCCCEEHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]