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The map label for this gene is gidA

Identifier: 49188320

GI number: 49188320

Start: 5222976

End: 5224865

Strand: Reverse

Name: gidA

Synonym: BAS5336

Alternate gene names: 49188320

Gene position: 5224865-5222976 (Counterclockwise)

Preceding gene: 49188321

Following gene: 49188319

Centisome position: 99.93

GC content: 37.25

Gene sequence:

>1890_bases
ATGGGATACAATGCCGGTTCATACGATGTCATAGTAATCGGTGCAGGTCATGCAGGATGTGAAGCTGGTCTTGCGGCAGC
ACGAATGGGCTCCAAAACATTAATGTTAACAATTAACTTAGATATGGTAGCGTTTATGCCATGTAACCCTTCTGTTGGTG
GACCAGCAAAAGGGATTGTTGTTCGTGAAATTGATGCATTAGGCGGAGAAATGGGGCGCAACATTGATAAAACGCATATT
CAAATGCGTATGTTAAATACAGGTAAAGGGCCAGCTGTACGTGCACTTCGAGCACAAGCAGATAAGTTCTCTTACCAGCA
TGAATTAAAGAAAACAATTGAAGAAACACCAAACTTAACGTTGTTCCAAGGAATGGTAGAGCGTTTAATTGTTGAAGATG
GCGAATGTAAAGGTGTAATTACGCAAGCTGGTGCTGAATATACAGCAAAAACAGTCGTAATTACAACTGGAACGTTTTTA
CGTGGTGAGATTATTATGGGAGATTTGAAATATTCGAGTGGTCCAAATAATCAACAACCATCTATTACTTTATCAGAACA
TTTAGAGGAGCTTGGATTCGATCTTGTTAGATTTAAAACAGGTACACCTCCGCGTGTAAATAGTAATACGATTGATTACA
GTAAAACAGAAATTCAGCCAGGTGACGATAAACCACGAGCTTTCTCTTTTGAAACAACAAAATTTATTATGGATCAAATT
CCATGTTGGTTAACGTATACAAGTACGGAAACACATCGTTTAATAGATGAAAATCTACATCGCTCAGCTATGTATTCTGG
TATGATTAAGGGAACAGGGCCTAGATATTGTCCTTCAATTGAAGACAAGGTAGTAAGGTTTAATGATAAACCACGTCATC
AAATTTTCTTAGAGCCAGAAGGACGTAATACACAAGAAGTGTACGTACAAGGTTTATCTACAAGCTTACCAGAAGACGTA
CAGCGTGATATGCTTAGAACAATCCCTGGTTTAGAAAATGTTGAAATGATGCGTACAGGTTATGCAATTGAATATGATGC
AATTGTGCCAACGCAACTATGGCCAACACTTGAAACGAAAAAAATTAAAAATTTATATACAGCAGGACAAATTAACGGAA
CTTCTGGTTACGAAGAGGCAGCAGGACAAGGGCTTATGGCCGGAATTAATGCAGCATGTCGTTCTTTAGGTAAAAAAGAA
GTTATTTTAGGTCGTGAAGATGCTTATATTGGTGTCTTAATTGATGACCTTGTAACGAAAGGAACGAATGAACCATATCG
TTTATTAACGTCTCGTGCAGAGTATCGTCTATTATTACGCCATGATAATGCGGATCTTCGTTTAACTGAAGTTGGTCATG
AAATTGGATTAATTACAGAGGAGCGCTATGAACGATTTACAAATAAAAAATTACAAATTGAACAAGAAAAGGAACGTTTA
AGCAGCATTATTATTAAACCACGTCCTGAAGTTCAAGAATTAATTCGCAATATTGGCGGAAGTGAATTGAAAGATGGGAT
ACGTGCAAGTGACTTATTACGTCGTCCAGAGATGACATATGAGCATATTCATCTTTTAGTACCAAGTGAAGTAGAATTAA
GTGATGAAGTTAAAGAACAAGTTGAAATTCAGATTAAGTACGAAGGATATATCGAAAAATCTTTACAACAAGTAGAGCGT
ATGAAGAAAATGGAAAATAAAAAAATCCCTGTAGATATTGATTATGATGCGATTTCTAGCCTTGCATCAGAAGCTAGACA
GAAATTGAAAGATGTTCGTCCGCTTTCAATGGGGCAAGCTTCACGTATATCTGGCGTAAATCCAGCGGACATTTCCATTT
TACTTGTGTATATTGAACAAGGAAAAATTGCGCGAGTATCGAACCAATAA

Upstream 100 bases:

>100_bases
GTTCATGAAAGCCTAATTGACCAGTTGTTCTCTCAATTTTGTTTAGGAAAATAATATAGTGATGTTTCAGAAAGATAAAG
ATTATTAGGAGGAATAAACA

Downstream 100 bases:

>100_bases
ATAGTAAGGAGATATTGTTAGATGAACATAGAACAATTTCAATCTATGCTAGAAGAGAAGGGTATCACCCTCTCTTCTAG
ACAGTTAGAGCAGTTCGAAA

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A

Number of amino acids: Translated: 629; Mature: 628

Protein sequence:

>629_residues
MGYNAGSYDVIVIGAGHAGCEAGLAAARMGSKTLMLTINLDMVAFMPCNPSVGGPAKGIVVREIDALGGEMGRNIDKTHI
QMRMLNTGKGPAVRALRAQADKFSYQHELKKTIEETPNLTLFQGMVERLIVEDGECKGVITQAGAEYTAKTVVITTGTFL
RGEIIMGDLKYSSGPNNQQPSITLSEHLEELGFDLVRFKTGTPPRVNSNTIDYSKTEIQPGDDKPRAFSFETTKFIMDQI
PCWLTYTSTETHRLIDENLHRSAMYSGMIKGTGPRYCPSIEDKVVRFNDKPRHQIFLEPEGRNTQEVYVQGLSTSLPEDV
QRDMLRTIPGLENVEMMRTGYAIEYDAIVPTQLWPTLETKKIKNLYTAGQINGTSGYEEAAGQGLMAGINAACRSLGKKE
VILGREDAYIGVLIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLRLTEVGHEIGLITEERYERFTNKKLQIEQEKERL
SSIIIKPRPEVQELIRNIGGSELKDGIRASDLLRRPEMTYEHIHLLVPSEVELSDEVKEQVEIQIKYEGYIEKSLQQVER
MKKMENKKIPVDIDYDAISSLASEARQKLKDVRPLSMGQASRISGVNPADISILLVYIEQGKIARVSNQ

Sequences:

>Translated_629_residues
MGYNAGSYDVIVIGAGHAGCEAGLAAARMGSKTLMLTINLDMVAFMPCNPSVGGPAKGIVVREIDALGGEMGRNIDKTHI
QMRMLNTGKGPAVRALRAQADKFSYQHELKKTIEETPNLTLFQGMVERLIVEDGECKGVITQAGAEYTAKTVVITTGTFL
RGEIIMGDLKYSSGPNNQQPSITLSEHLEELGFDLVRFKTGTPPRVNSNTIDYSKTEIQPGDDKPRAFSFETTKFIMDQI
PCWLTYTSTETHRLIDENLHRSAMYSGMIKGTGPRYCPSIEDKVVRFNDKPRHQIFLEPEGRNTQEVYVQGLSTSLPEDV
QRDMLRTIPGLENVEMMRTGYAIEYDAIVPTQLWPTLETKKIKNLYTAGQINGTSGYEEAAGQGLMAGINAACRSLGKKE
VILGREDAYIGVLIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLRLTEVGHEIGLITEERYERFTNKKLQIEQEKERL
SSIIIKPRPEVQELIRNIGGSELKDGIRASDLLRRPEMTYEHIHLLVPSEVELSDEVKEQVEIQIKYEGYIEKSLQQVER
MKKMENKKIPVDIDYDAISSLASEARQKLKDVRPLSMGQASRISGVNPADISILLVYIEQGKIARVSNQ
>Mature_628_residues
GYNAGSYDVIVIGAGHAGCEAGLAAARMGSKTLMLTINLDMVAFMPCNPSVGGPAKGIVVREIDALGGEMGRNIDKTHIQ
MRMLNTGKGPAVRALRAQADKFSYQHELKKTIEETPNLTLFQGMVERLIVEDGECKGVITQAGAEYTAKTVVITTGTFLR
GEIIMGDLKYSSGPNNQQPSITLSEHLEELGFDLVRFKTGTPPRVNSNTIDYSKTEIQPGDDKPRAFSFETTKFIMDQIP
CWLTYTSTETHRLIDENLHRSAMYSGMIKGTGPRYCPSIEDKVVRFNDKPRHQIFLEPEGRNTQEVYVQGLSTSLPEDVQ
RDMLRTIPGLENVEMMRTGYAIEYDAIVPTQLWPTLETKKIKNLYTAGQINGTSGYEEAAGQGLMAGINAACRSLGKKEV
ILGREDAYIGVLIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLRLTEVGHEIGLITEERYERFTNKKLQIEQEKERLS
SIIIKPRPEVQELIRNIGGSELKDGIRASDLLRRPEMTYEHIHLLVPSEVELSDEVKEQVEIQIKYEGYIEKSLQQVERM
KKMENKKIPVDIDYDAISSLASEARQKLKDVRPLSMGQASRISGVNPADISILLVYIEQGKIARVSNQ

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family

Homologues:

Organism=Homo sapiens, GI74024895, Length=630, Percent_Identity=42.5396825396825, Blast_Score=457, Evalue=1e-128,
Organism=Homo sapiens, GI19882217, Length=655, Percent_Identity=40.9160305343511, Blast_Score=443, Evalue=1e-124,
Organism=Homo sapiens, GI183227703, Length=670, Percent_Identity=40, Blast_Score=437, Evalue=1e-122,
Organism=Escherichia coli, GI2367273, Length=622, Percent_Identity=52.411575562701, Blast_Score=635, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17534255, Length=627, Percent_Identity=40.1913875598086, Blast_Score=453, Evalue=1e-127,
Organism=Saccharomyces cerevisiae, GI6321202, Length=624, Percent_Identity=42.6282051282051, Blast_Score=477, Evalue=1e-135,
Organism=Drosophila melanogaster, GI24658174, Length=644, Percent_Identity=41.7701863354037, Blast_Score=469, Evalue=1e-132,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMG_BACAH (A0RLR1)

Other databases:

- EMBL:   CP000485
- RefSeq:   YP_897661.1
- ProteinModelPortal:   A0RLR1
- SMR:   A0RLR1
- STRING:   A0RLR1
- EnsemblBacteria:   EBBACT00000067915
- GeneID:   4544397
- GenomeReviews:   CP000485_GR
- KEGG:   btl:BALH_4990
- eggNOG:   COG0445
- GeneTree:   EBGT00050000001476
- HOGENOM:   HBG284774
- OMA:   GIQFRVL
- ProtClustDB:   PRK05192
- BioCyc:   BTHU412694:BALH_4990-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00129
- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595
- TIGRFAMs:   TIGR00136

Pfam domain/function: PF01134 GIDA

EC number: NA

Molecular weight: Translated: 70336; Mature: 70204

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: BINDING 126-126 BINDING 181-181 BINDING 370-370

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGYNAGSYDVIVIGAGHAGCEAGLAAARMGSKTLMLTINLDMVAFMPCNPSVGGPAKGIV
CCCCCCCEEEEEEECCCCCHHHHHHHHHCCCCEEEEEEEEEEEEEECCCCCCCCCCCCEE
VREIDALGGEMGRNIDKTHIQMRMLNTGKGPAVRALRAQADKFSYQHELKKTIEETPNLT
EEEHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
LFQGMVERLIVEDGECKGVITQAGAEYTAKTVVITTGTFLRGEIIMGDLKYSSGPNNQQP
HHHHHHHHHHHCCCCCCEEEEECCCCEEEEEEEEEECCEEECEEEEEEEEECCCCCCCCC
SITLSEHLEELGFDLVRFKTGTPPRVNSNTIDYSKTEIQPGDDKPRAFSFETTKFIMDQI
CEEHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEECHHHHHHHHCC
PCWLTYTSTETHRLIDENLHRSAMYSGMIKGTGPRYCPSIEDKVVRFNDKPRHQIFLEPE
CEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCEEEEECCC
GRNTQEVYVQGLSTSLPEDVQRDMLRTIPGLENVEMMRTGYAIEYDAIVPTQLWPTLETK
CCCHHHHHEECCCCCCCHHHHHHHHHHCCCCCCCHHHHCCCEEEECCCCCCHHCCCCHHH
KIKNLYTAGQINGTSGYEEAAGQGLMAGINAACRSLGKKEVILGREDAYIGVLIDDLVTK
HHHHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHHCCCCCEEEECCCCCEEEEEHHHHHHC
GTNEPYRLLTSRAEYRLLLRHDNADLRLTEVGHEIGLITEERYERFTNKKLQIEQEKERL
CCCCCHHHHHCCCCEEEEEEECCCCEEEEECCCHHCCEEHHHHHHHCCCCEEEHHHHHHH
SSIIIKPRPEVQELIRNIGGSELKDGIRASDLLRRPEMTYEHIHLLVPSEVELSDEVKEQ
HHEECCCCHHHHHHHHHCCCHHHHHCCCHHHHHHCCCCCEEEEEEECCCCCCHHHHHHCC
VEIQIKYEGYIEKSLQQVERMKKMENKKIPVDIDYDAISSLASEARQKLKDVRPLSMGQA
EEEEEEECHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHHHHHCCCCCCCCC
SRISGVNPADISILLVYIEQGKIARVSNQ
HHCCCCCCCCEEEEEEEEECCCEEECCCC
>Mature Secondary Structure 
GYNAGSYDVIVIGAGHAGCEAGLAAARMGSKTLMLTINLDMVAFMPCNPSVGGPAKGIV
CCCCCCEEEEEEECCCCCHHHHHHHHHCCCCEEEEEEEEEEEEEECCCCCCCCCCCCEE
VREIDALGGEMGRNIDKTHIQMRMLNTGKGPAVRALRAQADKFSYQHELKKTIEETPNLT
EEEHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
LFQGMVERLIVEDGECKGVITQAGAEYTAKTVVITTGTFLRGEIIMGDLKYSSGPNNQQP
HHHHHHHHHHHCCCCCCEEEEECCCCEEEEEEEEEECCEEECEEEEEEEEECCCCCCCCC
SITLSEHLEELGFDLVRFKTGTPPRVNSNTIDYSKTEIQPGDDKPRAFSFETTKFIMDQI
CEEHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEECHHHHHHHHCC
PCWLTYTSTETHRLIDENLHRSAMYSGMIKGTGPRYCPSIEDKVVRFNDKPRHQIFLEPE
CEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCEEEEECCC
GRNTQEVYVQGLSTSLPEDVQRDMLRTIPGLENVEMMRTGYAIEYDAIVPTQLWPTLETK
CCCHHHHHEECCCCCCCHHHHHHHHHHCCCCCCCHHHHCCCEEEECCCCCCHHCCCCHHH
KIKNLYTAGQINGTSGYEEAAGQGLMAGINAACRSLGKKEVILGREDAYIGVLIDDLVTK
HHHHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHHCCCCCEEEECCCCCEEEEEHHHHHHC
GTNEPYRLLTSRAEYRLLLRHDNADLRLTEVGHEIGLITEERYERFTNKKLQIEQEKERL
CCCCCHHHHHCCCCEEEEEEECCCCEEEEECCCHHCCEEHHHHHHHCCCCEEEHHHHHHH
SSIIIKPRPEVQELIRNIGGSELKDGIRASDLLRRPEMTYEHIHLLVPSEVELSDEVKEQ
HHEECCCCHHHHHHHHHCCCHHHHHCCCHHHHHHCCCCCEEEEEEECCCCCCHHHHHHCC
VEIQIKYEGYIEKSLQQVERMKKMENKKIPVDIDYDAISSLASEARQKLKDVRPLSMGQA
EEEEEEECHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHHHHHCCCCCCCCC
SRISGVNPADISILLVYIEQGKIARVSNQ
HHCCCCCCCCEEEEEEEEECCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA