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The map label for this gene is trmE [H]

Identifier: 49188321

GI number: 49188321

Start: 5224912

End: 5226288

Strand: Reverse

Name: trmE [H]

Synonym: BAS5337

Alternate gene names: 49188321

Gene position: 5226288-5224912 (Counterclockwise)

Preceding gene: 49188322

Following gene: 49188320

Centisome position: 99.95

GC content: 36.75

Gene sequence:

>1377_bases
ATGGAATTTGATACAATTGCCGCGATTTCCACAGCGCTTGGAGAGGGTGCAATTGCCATTGTTCGAGTAAGTGGAGATGA
TGCGGTTGAAAAAGTTAATCGTATTTTTAAAGGGAAGGATTTAACAGAGGTTCCTTCTCACACGATTCATTATGGTCATA
TTGTGGATTTAGATACAAATCAGGTTATTGAAGAAGTGATGGTATCTATTATGCGTGCACCAAGGACTTTTACACGTGAA
AATATAGTAGAAATTAACTGTCACGGTGGACTTGTTTCGGTAAATAAAGTATTGCAGCTTATTTTAGCGCAAGGGGTACG
ATTAGCAGAGCCTGGTGAATTTACAAAACGTGCTTTTTTAAATGGCCGTATTGATTTATCACAAGCAGAAGCTGTTATGG
ACTTAATCCGTGCAAAAACAGATCGTGCAATGAACGTAGCGATTAATCAAATGGAAGGACGATTATCTAAATTAATTGGT
CGTCTGCGCCAAGATATATTAGAAACGTTAGCTCATGTTGAGGTAAACATAGATTACCCAGAATATGATGATGTGGAAGA
AATGACACATAATATTTTAATTGAGAAAGCTACACATGTTCGTGCTGAAATTGCAAAGATATTAGAAACATCGAAGCAAG
GAAAGATTTTACGTGAAGGTATTGCTACTGCAATCATCGGTAGACCTAATGTTGGGAAGTCATCGCTATTAAATAGTCTC
GTTCAGGAGAAAAAGGCAATTGTAACTGATATTGCAGGAACAACTCGTGATGTTATTGAAGAGTACGTTAATGTGCGTGG
TGTACCACTTAAACTTATAGATACAGCGGGGATTCGTGAAACGGAAGATGTTGTTGAACGAATTGGCGTAGAGCGTTCAA
AAGAAATGATGAGTCAAGCTGATTTAGTGTTAGTTGTCGTTAATTATAGTGAAACTTTAACGAATGAGGATGAAGAGCTA
TTCCGTGCCGTACAAGGAAAAGATTTCATTGTTATTGTAAATAAGACAGATTTACCGCAAGCAATTGATATGGAACGTGT
TATAGAATTGGCAGCGGGAAATCGTGTTATTACAACATCGTTAATTGAAGAACAAGGAATAGATGAACTTGAAAAGGCAA
TAGCTGATTTATTCTTTGAAGGAACAATTGATTCTGCAGATGTAACATATGTTTCTAATGCGAGACATATTGGATTATTA
ACACAAGCAGGAAAAACAATTGGAGATGCAATCGAGGCGATAGAAAATGGTGTTCCAATTGATATGGTGCAAATTGATTT
AACAAGAACGTGGGAAATACTTGGTGAAATTACTGGTGATACCGTTCATGAAAGCCTAATTGACCAGTTGTTCTCTCAAT
TTTGTTTAGGAAAATAA

Upstream 100 bases:

>100_bases
TTATTCTTTTGTAGATAGGTTCGTTATGGTATTCTAATAATTTAGTGAAGAAAATAATCATGTTGAAATAGAGATAAATA
AGCAAGTGAGGTGAAAGGAC

Downstream 100 bases:

>100_bases
TATAGTGATGTTTCAGAAAGATAAAGATTATTAGGAGGAATAAACAATGGGATACAATGCCGGTTCATACGATGTCATAG
TAATCGGTGCAGGTCATGCA

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MEFDTIAAISTALGEGAIAIVRVSGDDAVEKVNRIFKGKDLTEVPSHTIHYGHIVDLDTNQVIEEVMVSIMRAPRTFTRE
NIVEINCHGGLVSVNKVLQLILAQGVRLAEPGEFTKRAFLNGRIDLSQAEAVMDLIRAKTDRAMNVAINQMEGRLSKLIG
RLRQDILETLAHVEVNIDYPEYDDVEEMTHNILIEKATHVRAEIAKILETSKQGKILREGIATAIIGRPNVGKSSLLNSL
VQEKKAIVTDIAGTTRDVIEEYVNVRGVPLKLIDTAGIRETEDVVERIGVERSKEMMSQADLVLVVVNYSETLTNEDEEL
FRAVQGKDFIVIVNKTDLPQAIDMERVIELAAGNRVITTSLIEEQGIDELEKAIADLFFEGTIDSADVTYVSNARHIGLL
TQAGKTIGDAIEAIENGVPIDMVQIDLTRTWEILGEITGDTVHESLIDQLFSQFCLGK

Sequences:

>Translated_458_residues
MEFDTIAAISTALGEGAIAIVRVSGDDAVEKVNRIFKGKDLTEVPSHTIHYGHIVDLDTNQVIEEVMVSIMRAPRTFTRE
NIVEINCHGGLVSVNKVLQLILAQGVRLAEPGEFTKRAFLNGRIDLSQAEAVMDLIRAKTDRAMNVAINQMEGRLSKLIG
RLRQDILETLAHVEVNIDYPEYDDVEEMTHNILIEKATHVRAEIAKILETSKQGKILREGIATAIIGRPNVGKSSLLNSL
VQEKKAIVTDIAGTTRDVIEEYVNVRGVPLKLIDTAGIRETEDVVERIGVERSKEMMSQADLVLVVVNYSETLTNEDEEL
FRAVQGKDFIVIVNKTDLPQAIDMERVIELAAGNRVITTSLIEEQGIDELEKAIADLFFEGTIDSADVTYVSNARHIGLL
TQAGKTIGDAIEAIENGVPIDMVQIDLTRTWEILGEITGDTVHESLIDQLFSQFCLGK
>Mature_458_residues
MEFDTIAAISTALGEGAIAIVRVSGDDAVEKVNRIFKGKDLTEVPSHTIHYGHIVDLDTNQVIEEVMVSIMRAPRTFTRE
NIVEINCHGGLVSVNKVLQLILAQGVRLAEPGEFTKRAFLNGRIDLSQAEAVMDLIRAKTDRAMNVAINQMEGRLSKLIG
RLRQDILETLAHVEVNIDYPEYDDVEEMTHNILIEKATHVRAEIAKILETSKQGKILREGIATAIIGRPNVGKSSLLNSL
VQEKKAIVTDIAGTTRDVIEEYVNVRGVPLKLIDTAGIRETEDVVERIGVERSKEMMSQADLVLVVVNYSETLTNEDEEL
FRAVQGKDFIVIVNKTDLPQAIDMERVIELAAGNRVITTSLIEEQGIDELEKAIADLFFEGTIDSADVTYVSNARHIGLL
TQAGKTIGDAIEAIENGVPIDMVQIDLTRTWEILGEITGDTVHESLIDQLFSQFCLGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=479, Percent_Identity=30.6889352818372, Blast_Score=204, Evalue=1e-52,
Organism=Homo sapiens, GI193082988, Length=508, Percent_Identity=28.5433070866142, Blast_Score=194, Evalue=2e-49,
Organism=Homo sapiens, GI193082993, Length=468, Percent_Identity=30.5555555555556, Blast_Score=192, Evalue=5e-49,
Organism=Escherichia coli, GI2367268, Length=464, Percent_Identity=33.8362068965517, Blast_Score=246, Evalue=3e-66,
Organism=Escherichia coli, GI87082120, Length=122, Percent_Identity=37.7049180327869, Blast_Score=80, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17507259, Length=464, Percent_Identity=29.0948275862069, Blast_Score=185, Evalue=4e-47,
Organism=Saccharomyces cerevisiae, GI6323665, Length=493, Percent_Identity=30.0202839756592, Blast_Score=204, Evalue=2e-53,
Organism=Drosophila melanogaster, GI45550813, Length=477, Percent_Identity=31.0272536687631, Blast_Score=201, Evalue=7e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 50576; Mature: 50576

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFDTIAAISTALGEGAIAIVRVSGDDAVEKVNRIFKGKDLTEVPSHTIHYGHIVDLDTN
CCCHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCEEECEEEECCHH
QVIEEVMVSIMRAPRTFTRENIVEINCHGGLVSVNKVLQLILAQGVRLAEPGEFTKRAFL
HHHHHHHHHHHHCCCHHCCCCEEEEECCCCEEHHHHHHHHHHHCCCCCCCCCHHHHHHHH
NGRIDLSQAEAVMDLIRAKTDRAMNVAINQMEGRLSKLIGRLRQDILETLAHVEVNIDYP
CCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCC
EYDDVEEMTHNILIEKATHVRAEIAKILETSKQGKILREGIATAIIGRPNVGKSSLLNSL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHH
VQEKKAIVTDIAGTTRDVIEEYVNVRGVPLKLIDTAGIRETEDVVERIGVERSKEMMSQA
HHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHCCHHHHHHHCCC
DLVLVVVNYSETLTNEDEELFRAVQGKDFIVIVNKTDLPQAIDMERVIELAAGNRVITTS
CEEEEEEECHHHHCCCHHHHHHHHCCCCEEEEEECCCCCCHHCHHHHHHHHCCCEEEEHH
LIEEQGIDELEKAIADLFFEGTIDSADVTYVSNARHIGLLTQAGKTIGDAIEAIENGVPI
HHHHCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCEEHHHHCCHHHHHHHHHHHCCCCE
DMVQIDLTRTWEILGEITGDTVHESLIDQLFSQFCLGK
EEEEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEFDTIAAISTALGEGAIAIVRVSGDDAVEKVNRIFKGKDLTEVPSHTIHYGHIVDLDTN
CCCHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCEEECEEEECCHH
QVIEEVMVSIMRAPRTFTRENIVEINCHGGLVSVNKVLQLILAQGVRLAEPGEFTKRAFL
HHHHHHHHHHHHCCCHHCCCCEEEEECCCCEEHHHHHHHHHHHCCCCCCCCCHHHHHHHH
NGRIDLSQAEAVMDLIRAKTDRAMNVAINQMEGRLSKLIGRLRQDILETLAHVEVNIDYP
CCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCC
EYDDVEEMTHNILIEKATHVRAEIAKILETSKQGKILREGIATAIIGRPNVGKSSLLNSL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHH
VQEKKAIVTDIAGTTRDVIEEYVNVRGVPLKLIDTAGIRETEDVVERIGVERSKEMMSQA
HHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHCCHHHHHHHCCC
DLVLVVVNYSETLTNEDEELFRAVQGKDFIVIVNKTDLPQAIDMERVIELAAGNRVITTS
CEEEEEEECHHHHCCCHHHHHHHHCCCCEEEEEECCCCCCHHCHHHHHHHHCCCEEEEHH
LIEEQGIDELEKAIADLFFEGTIDSADVTYVSNARHIGLLTQAGKTIGDAIEAIENGVPI
HHHHCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCEEHHHHCCHHHHHHHHHHHCCCCE
DMVQIDLTRTWEILGEITGDTVHESLIDQLFSQFCLGK
EEEEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA