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The map label for this gene is prfA [H]

Identifier: 49188164

GI number: 49188164

Start: 5057771

End: 5058847

Strand: Reverse

Name: prfA [H]

Synonym: BAS5178

Alternate gene names: 49188164

Gene position: 5058847-5057771 (Counterclockwise)

Preceding gene: 49188165

Following gene: 49188163

Centisome position: 96.75

GC content: 40.02

Gene sequence:

>1077_bases
GTGAATGATGTGTTAGATCGTTTGCAAGCTGTAGAAAATCGTTATGAGAAGTTAAATGAATTGCTAAGCGACCCAGCAAT
TATTAGTGATTCAAATAAGCTTCGTGAATATTCAAAGGAACAGTCTGATATACAGGAAACGGTAGAGGTGTATCGTGAGT
ATAAGGATGTTCGTGAGCAATTAAAAGATGCGAAAGCAATGTTAGAAGATAAGTTAGACGCAGAAATGCGTGAAATGGTA
AAAGAAGAGGTTTCTGAGCTAGAATCACAAGAAAAAACATTATCAGAGCGTCTGAAAATTTTACTTGTACCAAAAGATCC
TAACGATGATAAGAACGTTATCGTTGAGGTTCGTGGAGCTGCCGGTGGTGACGAGGCTGCTTTATTTGCTGGTGATTTAT
ACCGTATGTATAGCCGTTACGCTGAGGTACAAGGTTGGAAAACGGAGATTATCGAGGCTAGCTATACAGAGTTAGGTGGA
TATAAAGAGATTATCTTTATGATTAACGGTAAAGGTGCTTTCGCGAAGTTGAAATTTGAGAATGGCGCTCACCGTGTACA
ACGTGTTCCTGAAACGGAATCTGGTGGACGTATTCATACATCTACAGCAACTGTAGCTGTATTACCAGAGGCAGAAGAAG
TAGAAATTGATATTCATGAGAAAGATGTTCGTGTTGATACATTCGCTTCTAGTGGACCTGGTGGACAGAGCGTTAATACA
ACGATGTCAGCGGTACGTTTAACGCATTTACCGACTGGTGTAGTTGTATCGTGTCAGGATGAGAAATCACAAATTAAGAA
TAAAGAAAAAGCGATGAAAGTATTACGCGCACGTGTTTATGATAAGTTTAGACAAGAAGCACAAGCTGAGTATGATCAAA
ACCGTAAACAAGCTGTTGGTACGGGTGATCGTTCAGAGCGTATTCGTACGTATAACTTCCCGCAAAACCGTGTTACAGAC
CATCGAATCGGTTTAACGATTCAAAAGCTAGATCAAATCTTACAAGGTAAGTTAGATGATTTCATCAATGCCTTAGTGAT
GGAAGATCAGGCTCAAAGGATGGAGGCAGCTGAGTAA

Upstream 100 bases:

>100_bases
GCTGGTCAGAGCTGAACGAGCCACCGCCACTTTTAAATATAATTTGCGTTTTTTTGAGTAAGTACCATATACTATTTGTA
TTAGATACTAGGATGTAGAG

Downstream 100 bases:

>100_bases
TGCGTGTCTATGAAGCCCTGAAATGGGCTTCTTCTTTTTTACAGGAGAATGGGCGAGATGAAAATGCGGGAGAAATTGTC
CTTTGTCATGTATTAAAGAC

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MNDVLDRLQAVENRYEKLNELLSDPAIISDSNKLREYSKEQSDIQETVEVYREYKDVREQLKDAKAMLEDKLDAEMREMV
KEEVSELESQEKTLSERLKILLVPKDPNDDKNVIVEVRGAAGGDEAALFAGDLYRMYSRYAEVQGWKTEIIEASYTELGG
YKEIIFMINGKGAFAKLKFENGAHRVQRVPETESGGRIHTSTATVAVLPEAEEVEIDIHEKDVRVDTFASSGPGGQSVNT
TMSAVRLTHLPTGVVVSCQDEKSQIKNKEKAMKVLRARVYDKFRQEAQAEYDQNRKQAVGTGDRSERIRTYNFPQNRVTD
HRIGLTIQKLDQILQGKLDDFINALVMEDQAQRMEAAE

Sequences:

>Translated_358_residues
MNDVLDRLQAVENRYEKLNELLSDPAIISDSNKLREYSKEQSDIQETVEVYREYKDVREQLKDAKAMLEDKLDAEMREMV
KEEVSELESQEKTLSERLKILLVPKDPNDDKNVIVEVRGAAGGDEAALFAGDLYRMYSRYAEVQGWKTEIIEASYTELGG
YKEIIFMINGKGAFAKLKFENGAHRVQRVPETESGGRIHTSTATVAVLPEAEEVEIDIHEKDVRVDTFASSGPGGQSVNT
TMSAVRLTHLPTGVVVSCQDEKSQIKNKEKAMKVLRARVYDKFRQEAQAEYDQNRKQAVGTGDRSERIRTYNFPQNRVTD
HRIGLTIQKLDQILQGKLDDFINALVMEDQAQRMEAAE
>Mature_358_residues
MNDVLDRLQAVENRYEKLNELLSDPAIISDSNKLREYSKEQSDIQETVEVYREYKDVREQLKDAKAMLEDKLDAEMREMV
KEEVSELESQEKTLSERLKILLVPKDPNDDKNVIVEVRGAAGGDEAALFAGDLYRMYSRYAEVQGWKTEIIEASYTELGG
YKEIIFMINGKGAFAKLKFENGAHRVQRVPETESGGRIHTSTATVAVLPEAEEVEIDIHEKDVRVDTFASSGPGGQSVNT
TMSAVRLTHLPTGVVVSCQDEKSQIKNKEKAMKVLRARVYDKFRQEAQAEYDQNRKQAVGTGDRSERIRTYNFPQNRVTD
HRIGLTIQKLDQILQGKLDDFINALVMEDQAQRMEAAE

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=288, Percent_Identity=45.1388888888889, Blast_Score=265, Evalue=7e-71,
Organism=Homo sapiens, GI34577120, Length=342, Percent_Identity=37.1345029239766, Blast_Score=229, Evalue=3e-60,
Organism=Homo sapiens, GI166795305, Length=207, Percent_Identity=40.5797101449275, Blast_Score=171, Evalue=1e-42,
Organism=Escherichia coli, GI1787462, Length=357, Percent_Identity=51.2605042016807, Blast_Score=365, Evalue=1e-102,
Organism=Escherichia coli, GI2367172, Length=338, Percent_Identity=36.094674556213, Blast_Score=209, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI17542784, Length=281, Percent_Identity=38.7900355871886, Blast_Score=191, Evalue=7e-49,
Organism=Saccharomyces cerevisiae, GI6321295, Length=334, Percent_Identity=43.4131736526946, Blast_Score=243, Evalue=4e-65,
Organism=Drosophila melanogaster, GI19921226, Length=295, Percent_Identity=42.7118644067797, Blast_Score=235, Evalue=4e-62,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40685; Mature: 40685

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDVLDRLQAVENRYEKLNELLSDPAIISDSNKLREYSKEQSDIQETVEVYREYKDVREQ
CCHHHHHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKDAKAMLEDKLDAEMREMVKEEVSELESQEKTLSERLKILLVPKDPNDDKNVIVEVRGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCEEEEEEECC
AGGDEAALFAGDLYRMYSRYAEVQGWKTEIIEASYTELGGYKEIIFMINGKGAFAKLKFE
CCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEEECCCCEEEEEEC
NGAHRVQRVPETESGGRIHTSTATVAVLPEAEEVEIDIHEKDVRVDTFASSGPGGQSVNT
CCHHHHHCCCCCCCCCEEEECCEEEEECCCCCEEEEEEECCCCEEEECCCCCCCCCCHHH
TMSAVRLTHLPTGVVVSCQDEKSQIKNKEKAMKVLRARVYDKFRQEAQAEYDQNRKQAVG
HHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCC
TGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILQGKLDDFINALVMEDQAQRMEAAE
CCCCCCCCEECCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNDVLDRLQAVENRYEKLNELLSDPAIISDSNKLREYSKEQSDIQETVEVYREYKDVREQ
CCHHHHHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKDAKAMLEDKLDAEMREMVKEEVSELESQEKTLSERLKILLVPKDPNDDKNVIVEVRGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCEEEEEEECC
AGGDEAALFAGDLYRMYSRYAEVQGWKTEIIEASYTELGGYKEIIFMINGKGAFAKLKFE
CCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEEECCCCEEEEEEC
NGAHRVQRVPETESGGRIHTSTATVAVLPEAEEVEIDIHEKDVRVDTFASSGPGGQSVNT
CCHHHHHCCCCCCCCCEEEECCEEEEECCCCCEEEEEEECCCCEEEECCCCCCCCCCHHH
TMSAVRLTHLPTGVVVSCQDEKSQIKNKEKAMKVLRARVYDKFRQEAQAEYDQNRKQAVG
HHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCC
TGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILQGKLDDFINALVMEDQAQRMEAAE
CCCCCCCCEECCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]