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The map label for this gene is ndhD1 [H]

Identifier: 49188127

GI number: 49188127

Start: 5025425

End: 5026918

Strand: Reverse

Name: ndhD1 [H]

Synonym: BAS5141

Alternate gene names: 49188127

Gene position: 5026918-5025425 (Counterclockwise)

Preceding gene: 49188128

Following gene: 49188126

Centisome position: 96.14

GC content: 41.57

Gene sequence:

>1494_bases
ATGAATGATTTGTTATTAACGTTCTTCATTTTTTCCCCGCTTTTAGGAATTCTCTTACTTGCATTAACACCGAAAAAAGA
ATCGCGTACAGTGCGAGCGCTCGGTTTATTTGGAACAGTGCTTCCATTTGGAATGGCTATCGTCCTCGCTTGTACATATG
CTTCTGGAAGGGATTTGTCTCTATTTGATGAAAAAGTGAAATGGATTAAATTCGGGGATTTTGCAGCGGTAGATAAAAGG
TGGTTTTCCATTTATTACGAACTTGGTATAGATGGTTTATCGCTCGTTATGATGGTGCTGACGGCCCTTCTAGCGATGCT
TGCAGCATTTACAATTAAAAGAAATTTGAAAGCATTTTATATGCTGTTACTCATGTTAGAAATAGGGATGCTCGGCGTCT
TCGCTGCTCAAAATTTAATGTTGTTCTTTATCTTCTTTGAAATTACATTGCCGCCGATGTTTTTATTAATTGGGAAGTGG
GGGAAATTGTCGAGTGAAAAGGCTGCTTATAGTTATTTAATATATAACGGTATTGGCTCCGCTATTTTACTCATCGTTTT
CTCGGTTTTATTTGCGAAAACAGGTACAACAAATATTACAGAACTGAAAGAAATATTAGCGAGTGTAGGTGCTGGGGGAG
GAATGGTTGTCCCAAGTAGCTTACAGTTCGGCTTGTTTCTTGCCATAATGATTGCTTTCGCAATTAAATTACCCGTTTTC
CCGTTACATCGCTGGATGGTCAATGTACATATTGAAGCGCATCCGGCTGTAGTAATGCTTCATGCAGGTGTTTTACTGAA
GATTGGAGCGTACGGTATAATTCGCTTCGGGCAGGGGCTATTCCCGGAATACTTTCGCGAGTTTGCAACGCTCATTGCGA
TTTTAGGGGTTATCAATTTATTGTACGGAGCCTTCTTAGCGCTCATTCAAACAGACTTTCGGAAGGTGCTTGCTTATTCT
AGTATTTCGCATATGGGTATTGTATTAATGGGCCTTGCGGCGTTAAATGCACCAGGTACACAAGGGGCACTATTCCAAGT
TGTGTCGCATGGCTTAATTGCAGCCTTACTCTTTTTCTTACTTGGTGTTATAGAACAGCGTTTTGGAACGTCGGATATTA
CAGCGCTTGGCGGACTTGCAAAAAGTGTACCAGTACTTAGCGGTTTCTTCTTAGCGGGAGGAATGGCATCGCTTGGATTG
CCGGGAATGTCTGGATTTGTTAGCGAGTTTCTTGCCTTTCTCGGTTTATTCCAAGGAGAGCCAGTCATTGCTGCGGCCGG
AGTACTTGGCATCATTTTAACAGCTGTATACGTATTAAGAGCAACACTGCAAGTAACATTTGGTAAGAAAGAGTGGGAAG
CGAAAGCTGATATACACGGATGGGAGTATGTTCCTATCTTGCTACTTATCTTCTGCATTATTGCAATTGGCGTAATGCCA
GAAATACTAGGGGATCCGCTTCAAAATACATTGAAAACATTGGGGGTGAAGTAG

Upstream 100 bases:

>100_bases
CTAGACTCCAAAACGGAAATGTACAAGTGTACGGGACTGTAACAGCTGTTTCACTTGCAGTGCTCTTCATTATTTTGCTC
TATACGGGAGCGGATTTACG

Downstream 100 bases:

>100_bases
GATGGATATGAATACGTTACTTAGCTTATCGTGGCATCTCATGGTGCCAGAATTCATCATTCTCGGGGCTGCCATCCTTC
TTTCCATATGTGATTTGTTT

Product: NADH dehydrogenase subunit M

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase I, chain 4 1; NDH-1, chain 4 1 [H]

Number of amino acids: Translated: 497; Mature: 497

Protein sequence:

>497_residues
MNDLLLTFFIFSPLLGILLLALTPKKESRTVRALGLFGTVLPFGMAIVLACTYASGRDLSLFDEKVKWIKFGDFAAVDKR
WFSIYYELGIDGLSLVMMVLTALLAMLAAFTIKRNLKAFYMLLLMLEIGMLGVFAAQNLMLFFIFFEITLPPMFLLIGKW
GKLSSEKAAYSYLIYNGIGSAILLIVFSVLFAKTGTTNITELKEILASVGAGGGMVVPSSLQFGLFLAIMIAFAIKLPVF
PLHRWMVNVHIEAHPAVVMLHAGVLLKIGAYGIIRFGQGLFPEYFREFATLIAILGVINLLYGAFLALIQTDFRKVLAYS
SISHMGIVLMGLAALNAPGTQGALFQVVSHGLIAALLFFLLGVIEQRFGTSDITALGGLAKSVPVLSGFFLAGGMASLGL
PGMSGFVSEFLAFLGLFQGEPVIAAAGVLGIILTAVYVLRATLQVTFGKKEWEAKADIHGWEYVPILLLIFCIIAIGVMP
EILGDPLQNTLKTLGVK

Sequences:

>Translated_497_residues
MNDLLLTFFIFSPLLGILLLALTPKKESRTVRALGLFGTVLPFGMAIVLACTYASGRDLSLFDEKVKWIKFGDFAAVDKR
WFSIYYELGIDGLSLVMMVLTALLAMLAAFTIKRNLKAFYMLLLMLEIGMLGVFAAQNLMLFFIFFEITLPPMFLLIGKW
GKLSSEKAAYSYLIYNGIGSAILLIVFSVLFAKTGTTNITELKEILASVGAGGGMVVPSSLQFGLFLAIMIAFAIKLPVF
PLHRWMVNVHIEAHPAVVMLHAGVLLKIGAYGIIRFGQGLFPEYFREFATLIAILGVINLLYGAFLALIQTDFRKVLAYS
SISHMGIVLMGLAALNAPGTQGALFQVVSHGLIAALLFFLLGVIEQRFGTSDITALGGLAKSVPVLSGFFLAGGMASLGL
PGMSGFVSEFLAFLGLFQGEPVIAAAGVLGIILTAVYVLRATLQVTFGKKEWEAKADIHGWEYVPILLLIFCIIAIGVMP
EILGDPLQNTLKTLGVK
>Mature_497_residues
MNDLLLTFFIFSPLLGILLLALTPKKESRTVRALGLFGTVLPFGMAIVLACTYASGRDLSLFDEKVKWIKFGDFAAVDKR
WFSIYYELGIDGLSLVMMVLTALLAMLAAFTIKRNLKAFYMLLLMLEIGMLGVFAAQNLMLFFIFFEITLPPMFLLIGKW
GKLSSEKAAYSYLIYNGIGSAILLIVFSVLFAKTGTTNITELKEILASVGAGGGMVVPSSLQFGLFLAIMIAFAIKLPVF
PLHRWMVNVHIEAHPAVVMLHAGVLLKIGAYGIIRFGQGLFPEYFREFATLIAILGVINLLYGAFLALIQTDFRKVLAYS
SISHMGIVLMGLAALNAPGTQGALFQVVSHGLIAALLFFLLGVIEQRFGTSDITALGGLAKSVPVLSGFFLAGGMASLGL
PGMSGFVSEFLAFLGLFQGEPVIAAAGVLGIILTAVYVLRATLQVTFGKKEWEAKADIHGWEYVPILLLIFCIIAIGVMP
EILGDPLQNTLKTLGVK

Specific function: NDH-1 shuttles electrons from NAD(P)H, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton tran

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cellular thylakoid membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=340, Percent_Identity=29.7058823529412, Blast_Score=125, Evalue=8e-29,
Organism=Homo sapiens, GI251831117, Length=363, Percent_Identity=25.8953168044077, Blast_Score=91, Evalue=3e-18,
Organism=Escherichia coli, GI1788613, Length=463, Percent_Identity=34.9892008639309, Blast_Score=232, Evalue=5e-62,
Organism=Escherichia coli, GI1788614, Length=512, Percent_Identity=28.3203125, Blast_Score=128, Evalue=1e-30,
Organism=Escherichia coli, GI1788827, Length=326, Percent_Identity=28.2208588957055, Blast_Score=115, Evalue=8e-27,
Organism=Escherichia coli, GI1788831, Length=351, Percent_Identity=27.6353276353276, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI1788829, Length=428, Percent_Identity=22.8971962616822, Blast_Score=86, Evalue=7e-18,
Organism=Escherichia coli, GI2367154, Length=295, Percent_Identity=28.135593220339, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI145693160, Length=414, Percent_Identity=25.1207729468599, Blast_Score=78, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022997
- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.6.5.3

Molecular weight: Translated: 53825; Mature: 53825

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHCCHHHHHHHHHCCC
>Mature Secondary Structure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HHHCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]