Click here to switch to the map view.

The map label for this gene is yclK [H]

Identifier: 49188098

GI number: 49188098

Start: 4991171

End: 4992601

Strand: Reverse

Name: yclK [H]

Synonym: BAS5112

Alternate gene names: 49188098

Gene position: 4992601-4991171 (Counterclockwise)

Preceding gene: 49188099

Following gene: 49188097

Centisome position: 95.49

GC content: 38.71

Gene sequence:

>1431_bases
ATGTCTTTGAAAAGAAATATGGTGTTTGGAATCGTTGGACTGTTAATCCCGATATTCGTTCTTTTGTACGCAGTTGTTTA
TATTACGTTAGAAAAGAATATGTATCATAACGCAGCAAATTCTTTAGAAAAGTTAAGTGTAGAAGCTCAAATTTACACGA
TGAATTATTTAGAGAAGGAAGCAGAGGTAGAGACGCTCGGTCCGAATTCACTTTTAATTGCTTCGTATTTAGCGAAGCGC
ATGGATGTCCGTGTACAGATGATTGGGAAGAATGGAGATGTTGTTGCGGATACACAAAAAGGAGCACTTCTTCATCGGAA
TATTGATATTGAGAGTTCTTTGAAAGGGAAGAAATCTTATGTTTTTGAAGAGGGAGACCCGGCTCCAATTCTTTTGTTTT
CAAGCCCGGTTTATTATGGAAACGATGTCATTGGAAGTGTTCGTTTTATAAATGAGTTAACGGGTGAGAAGGAAGTTTTA
ACAAATGTCGGTTGGACGTTCTTAATGACGTCTCTTTGTCTCGTAGCTGCGGGTATTTTCTTTGCAATTCGTTTGGCAAA
GTCTCTCCATAAGCCGATTGATCAGTTAAGACAAATGGCGCACCGGCTTGCGAATGGTGATTATAAAAGTAAGATTGAGC
TAAATGAATATGTGGAAATTGCTCAGCTTTCCGCATCTTTTAATGCAATGGCCGATGGAATTGAACTGCATATTAAGCAA
TTGAAAGAGGAGAAAGAGAAGCAGAAAGATTTCCTAGACCGCATTACGCATGAGCTGAAAACACCGCTAACGGCAATTAT
TGGCTATGTGGATTTAATTCCGAAGTTAAAAGCGAAGGAAGATGTAAAGGAGAGCTTGCGTTATGTGGCTGTAGAAAGTG
AGCGTTTATTGTCACTTGTAGAGGAATTACTTAAGTCTTCAAAGTACGGAACGAGTACGTTTGAAATATCACCTACAGTT
GTGAATATAAAGGAATTAGCCGAAGAAGCAGTTTCAATTGTGAAACCTCGCCTGCAGAAATTTGAGATTGAAGTTATCAA
TGAGTTAACAGATGTACATGTTGTTGCAGATTTCGATAAGACGAAACAGATCTTCTTAAATGTGCTTGATAATGCGATAA
AATATAGCGATGCTACGCAAATTCGTATGAATGTCATTGTAAATGAACGTGAGGCAAAGGTTTTTGTTCATGATGATGGC
ATCGGTATCGATGAAGGTGTGCTGGCAGAGTGGAATGAATCGCCAGAAGGGAAGATTCTTCCTTCTAGTTACGGGCATGG
TTACGGTTTGTATATTTGTCAGGAGATTATGGATAAGCAGGGCGGAAGTATGCGGATTGAGAGTAGTGAAGAAATAGGCA
CTACCATATTTATTACATTCTTGCTTCCGAGACGAATGGAAGATATAAAAAATCTGAAAGCGGTTAAATGA

Upstream 100 bases:

>100_bases
TTATGGTGCAAAGATTGCGGAAGAAGTTAGGGAATAGCGGGGAATATATTAAGACAATTTATGGCGTTGGCTATAAACTA
GATTGTTAAGTGGTGATGGT

Downstream 100 bases:

>100_bases
TACATTTATGAAACAATTATGCGGTATTTTCGAAACAACGTTTTTTTATCTTGGAGGTAGGCAGAAATGAAAAGGCTATC
ATTTCAAATTCTTATGTTTG

Product: sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MSLKRNMVFGIVGLLIPIFVLLYAVVYITLEKNMYHNAANSLEKLSVEAQIYTMNYLEKEAEVETLGPNSLLIASYLAKR
MDVRVQMIGKNGDVVADTQKGALLHRNIDIESSLKGKKSYVFEEGDPAPILLFSSPVYYGNDVIGSVRFINELTGEKEVL
TNVGWTFLMTSLCLVAAGIFFAIRLAKSLHKPIDQLRQMAHRLANGDYKSKIELNEYVEIAQLSASFNAMADGIELHIKQ
LKEEKEKQKDFLDRITHELKTPLTAIIGYVDLIPKLKAKEDVKESLRYVAVESERLLSLVEELLKSSKYGTSTFEISPTV
VNIKELAEEAVSIVKPRLQKFEIEVINELTDVHVVADFDKTKQIFLNVLDNAIKYSDATQIRMNVIVNEREAKVFVHDDG
IGIDEGVLAEWNESPEGKILPSSYGHGYGLYICQEIMDKQGGSMRIESSEEIGTTIFITFLLPRRMEDIKNLKAVK

Sequences:

>Translated_476_residues
MSLKRNMVFGIVGLLIPIFVLLYAVVYITLEKNMYHNAANSLEKLSVEAQIYTMNYLEKEAEVETLGPNSLLIASYLAKR
MDVRVQMIGKNGDVVADTQKGALLHRNIDIESSLKGKKSYVFEEGDPAPILLFSSPVYYGNDVIGSVRFINELTGEKEVL
TNVGWTFLMTSLCLVAAGIFFAIRLAKSLHKPIDQLRQMAHRLANGDYKSKIELNEYVEIAQLSASFNAMADGIELHIKQ
LKEEKEKQKDFLDRITHELKTPLTAIIGYVDLIPKLKAKEDVKESLRYVAVESERLLSLVEELLKSSKYGTSTFEISPTV
VNIKELAEEAVSIVKPRLQKFEIEVINELTDVHVVADFDKTKQIFLNVLDNAIKYSDATQIRMNVIVNEREAKVFVHDDG
IGIDEGVLAEWNESPEGKILPSSYGHGYGLYICQEIMDKQGGSMRIESSEEIGTTIFITFLLPRRMEDIKNLKAVK
>Mature_475_residues
SLKRNMVFGIVGLLIPIFVLLYAVVYITLEKNMYHNAANSLEKLSVEAQIYTMNYLEKEAEVETLGPNSLLIASYLAKRM
DVRVQMIGKNGDVVADTQKGALLHRNIDIESSLKGKKSYVFEEGDPAPILLFSSPVYYGNDVIGSVRFINELTGEKEVLT
NVGWTFLMTSLCLVAAGIFFAIRLAKSLHKPIDQLRQMAHRLANGDYKSKIELNEYVEIAQLSASFNAMADGIELHIKQL
KEEKEKQKDFLDRITHELKTPLTAIIGYVDLIPKLKAKEDVKESLRYVAVESERLLSLVEELLKSSKYGTSTFEISPTVV
NIKELAEEAVSIVKPRLQKFEIEVINELTDVHVVADFDKTKQIFLNVLDNAIKYSDATQIRMNVIVNEREAKVFVHDDGI
GIDEGVLAEWNESPEGKILPSSYGHGYGLYICQEIMDKQGGSMRIESSEEIGTTIFITFLLPRRMEDIKNLKAVK

Specific function: Could be member of the two-component regulatory system yclK/yclJ. Potentially phosphorylates yclJ [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=288, Percent_Identity=25, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI87081816, Length=336, Percent_Identity=25.297619047619, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1789149, Length=338, Percent_Identity=23.0769230769231, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1788713, Length=273, Percent_Identity=24.9084249084249, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1788549, Length=213, Percent_Identity=27.2300469483568, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI145693157, Length=224, Percent_Identity=25, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI1786912, Length=227, Percent_Identity=24.2290748898678, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787894, Length=272, Percent_Identity=23.1617647058824, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI1790861, Length=386, Percent_Identity=22.7979274611399, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI48994928, Length=229, Percent_Identity=22.707423580786, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 53608; Mature: 53477

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLKRNMVFGIVGLLIPIFVLLYAVVYITLEKNMYHNAANSLEKLSVEAQIYTMNYLEKE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEHHHHHHH
AEVETLGPNSLLIASYLAKRMDVRVQMIGKNGDVVADTQKGALLHRNIDIESSLKGKKSY
CCCEECCCCHHHHHHHHHHHHCEEEEEECCCCCEEEECCCCEEEEECCCCHHCCCCCCCE
VFEEGDPAPILLFSSPVYYGNDVIGSVRFINELTGEKEVLTNVGWTFLMTSLCLVAAGIF
EEECCCCCEEEEEECCCEECCHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHH
FAIRLAKSLHKPIDQLRQMAHRLANGDYKSKIELNEYVEIAQLSASFNAMADGIELHIKQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKEEKEKQKDFLDRITHELKTPLTAIIGYVDLIPKLKAKEDVKESLRYVAVESERLLSLV
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHH
EELLKSSKYGTSTFEISPTVVNIKELAEEAVSIVKPRLQKFEIEVINELTDVHVVADFDK
HHHHHCCCCCCCEEEECCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCHH
TKQIFLNVLDNAIKYSDATQIRMNVIVNEREAKVFVHDDGIGIDEGVLAEWNESPEGKIL
HHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCEEEEECCCCCCCCCCCCCCCCCCCCCCC
PSSYGHGYGLYICQEIMDKQGGSMRIESSEEIGTTIFITFLLPRRMEDIKNLKAVK
CCCCCCCHHHHHHHHHHHCCCCCEEECCCCCCCCEEEHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SLKRNMVFGIVGLLIPIFVLLYAVVYITLEKNMYHNAANSLEKLSVEAQIYTMNYLEKE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEHHHHHHH
AEVETLGPNSLLIASYLAKRMDVRVQMIGKNGDVVADTQKGALLHRNIDIESSLKGKKSY
CCCEECCCCHHHHHHHHHHHHCEEEEEECCCCCEEEECCCCEEEEECCCCHHCCCCCCCE
VFEEGDPAPILLFSSPVYYGNDVIGSVRFINELTGEKEVLTNVGWTFLMTSLCLVAAGIF
EEECCCCCEEEEEECCCEECCHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHH
FAIRLAKSLHKPIDQLRQMAHRLANGDYKSKIELNEYVEIAQLSASFNAMADGIELHIKQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKEEKEKQKDFLDRITHELKTPLTAIIGYVDLIPKLKAKEDVKESLRYVAVESERLLSLV
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHH
EELLKSSKYGTSTFEISPTVVNIKELAEEAVSIVKPRLQKFEIEVINELTDVHVVADFDK
HHHHHCCCCCCCEEEECCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCHH
TKQIFLNVLDNAIKYSDATQIRMNVIVNEREAKVFVHDDGIGIDEGVLAEWNESPEGKIL
HHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCEEEEECCCCCCCCCCCCCCCCCCCCCCC
PSSYGHGYGLYICQEIMDKQGGSMRIESSEEIGTTIFITFLLPRRMEDIKNLKAVK
CCCCCCCHHHHHHHHHHHCCCCCEEECCCCCCCCEEEHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377 [H]