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The map label for this gene is phoB [C]

Identifier: 49188099

GI number: 49188099

Start: 4992613

End: 4993284

Strand: Reverse

Name: phoB [C]

Synonym: BAS5113

Alternate gene names: 49188099

Gene position: 4993284-4992613 (Counterclockwise)

Preceding gene: 49188100

Following gene: 49188098

Centisome position: 95.5

GC content: 40.62

Gene sequence:

>672_bases
ATGAAGATACTTGTAGTGGATGATGAGTCGAGTATTCGTAATTTGATTCGGATGCAGCTTGAGATGGAAGGATATGAAGT
GCTCACGGCGGCTGATGGGAGAGAAGTTTTAGAGAGATGGAATGAAGGGCCGGATGTTTTGATTTTGGATGTTATGCTTC
CAGATACAGATGGGTATGAATTACTTCGTTTGTTCCGTGAGAAGGATAGGGATATTCCAGTACTCATGTTAACTGCGAAG
AGTCAAATGAATGATAAATTGCTTGGTTTGCAGCTGGGCGCTGATGATTACGTGACGAAGCCATTTAATTATGCGGAGCT
TATTCTTCGGGTCAAGAATATGACGCGGCGTGTGAAGAAGAAAGAGGTAACGGTAAGTCATGAAGTAATCGAGGCAGGAG
AGATAACGATCTGTCCTAAGGAAAGAAAAGTGCATGTGAGTGAACAAGAGATTCAGTTAACGTACAGAGAGTTTAATTTA
TGTCAGTTGTTTGTTTCTAATCCGCAGCGTGTATTTATGAGAGATGAGTTACTTGAGAAAGTATGGGGATTTGAGTATAT
CGGAAATACGAGAGCGGTTGATATTATGGTGCAAAGATTGCGGAAGAAGTTAGGGAATAGCGGGGAATATATTAAGACAA
TTTATGGCGTTGGCTATAAACTAGATTGTTAA

Upstream 100 bases:

>100_bases
GTATTGTCGTTCAATATATGTTCGACAATACCCTCTCTTTTTTTGTATGATTTTTGCTATACTAAACTGTACTATAATAC
GGGATGGAAGGAGGAGAATA

Downstream 100 bases:

>100_bases
GTGGTGATGGTATGTCTTTGAAAAGAAATATGGTGTTTGGAATCGTTGGACTGTTAATCCCGATATTCGTTCTTTTGTAC
GCAGTTGTTTATATTACGTT

Product: DNA-binding response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 223; Mature: 223

Protein sequence:

>223_residues
MKILVVDDESSIRNLIRMQLEMEGYEVLTAADGREVLERWNEGPDVLILDVMLPDTDGYELLRLFREKDRDIPVLMLTAK
SQMNDKLLGLQLGADDYVTKPFNYAELILRVKNMTRRVKKKEVTVSHEVIEAGEITICPKERKVHVSEQEIQLTYREFNL
CQLFVSNPQRVFMRDELLEKVWGFEYIGNTRAVDIMVQRLRKKLGNSGEYIKTIYGVGYKLDC

Sequences:

>Translated_223_residues
MKILVVDDESSIRNLIRMQLEMEGYEVLTAADGREVLERWNEGPDVLILDVMLPDTDGYELLRLFREKDRDIPVLMLTAK
SQMNDKLLGLQLGADDYVTKPFNYAELILRVKNMTRRVKKKEVTVSHEVIEAGEITICPKERKVHVSEQEIQLTYREFNL
CQLFVSNPQRVFMRDELLEKVWGFEYIGNTRAVDIMVQRLRKKLGNSGEYIKTIYGVGYKLDC
>Mature_223_residues
MKILVVDDESSIRNLIRMQLEMEGYEVLTAADGREVLERWNEGPDVLILDVMLPDTDGYELLRLFREKDRDIPVLMLTAK
SQMNDKLLGLQLGADDYVTKPFNYAELILRVKNMTRRVKKKEVTVSHEVIEAGEITICPKERKVHVSEQEIQLTYREFNL
CQLFVSNPQRVFMRDELLEKVWGFEYIGNTRAVDIMVQRLRKKLGNSGEYIKTIYGVGYKLDC

Specific function: This Protein Is A Positive Regulator For The Phosphate Regulon. Transcription Of This Operon Is Positively Regulated By Phob And Phor When Phosphate Is Limited. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=227, Percent_Identity=37.0044052863436, Blast_Score=161, Evalue=4e-41,
Organism=Escherichia coli, GI1786784, Length=226, Percent_Identity=34.5132743362832, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1789809, Length=226, Percent_Identity=33.1858407079646, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1786911, Length=223, Percent_Identity=34.0807174887892, Blast_Score=130, Evalue=6e-32,
Organism=Escherichia coli, GI1789402, Length=222, Percent_Identity=34.6846846846847, Blast_Score=130, Evalue=9e-32,
Organism=Escherichia coli, GI1790860, Length=223, Percent_Identity=36.7713004484305, Blast_Score=129, Evalue=1e-31,
Organism=Escherichia coli, GI1787375, Length=222, Percent_Identity=32.4324324324324, Blast_Score=127, Evalue=6e-31,
Organism=Escherichia coli, GI2367329, Length=225, Percent_Identity=35.1111111111111, Blast_Score=122, Evalue=1e-29,
Organism=Escherichia coli, GI1790863, Length=229, Percent_Identity=34.061135371179, Blast_Score=121, Evalue=3e-29,
Organism=Escherichia coli, GI1788394, Length=228, Percent_Identity=33.3333333333333, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI87082012, Length=224, Percent_Identity=30.3571428571429, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI1787229, Length=228, Percent_Identity=32.4561403508772, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1790552, Length=222, Percent_Identity=30.6306306306306, Blast_Score=107, Evalue=6e-25,
Organism=Escherichia coli, GI145693140, Length=230, Percent_Identity=31.7391304347826, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788550, Length=167, Percent_Identity=29.3413173652695, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1790299, Length=121, Percent_Identity=33.8842975206612, Blast_Score=74, Evalue=6e-15,
Organism=Escherichia coli, GI1788713, Length=103, Percent_Identity=37.8640776699029, Blast_Score=67, Evalue=9e-13,
Organism=Escherichia coli, GI1788191, Length=111, Percent_Identity=37.8378378378378, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1787487, Length=101, Percent_Identity=34.6534653465347, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1790437, Length=108, Percent_Identity=35.1851851851852, Blast_Score=61, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 25996; Mature: 25996

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVVDDESSIRNLIRMQLEMEGYEVLTAADGREVLERWNEGPDVLILDVMLPDTDGYE
CEEEEECCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHCCCCCEEEEEEEECCCCCHH
LLRLFREKDRDIPVLMLTAKSQMNDKLLGLQLGADDYVTKPFNYAELILRVKNMTRRVKK
HHHHHHHCCCCCCEEEEECCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHH
KEVTVSHEVIEAGEITICPKERKVHVSEQEIQLTYREFNLCQLFVSNPQRVFMRDELLEK
HHHHHHHHHHCCCCEEECCCCCEEECCHHHEEEEEHHHHEEEEECCCCHHHHHHHHHHHH
VWGFEYIGNTRAVDIMVQRLRKKLGNSGEYIKTIYGVGYKLDC
HCCCHHCCCCHHHHHHHHHHHHHHCCCCCEEHHHHCCCEEECC
>Mature Secondary Structure
MKILVVDDESSIRNLIRMQLEMEGYEVLTAADGREVLERWNEGPDVLILDVMLPDTDGYE
CEEEEECCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHCCCCCEEEEEEEECCCCCHH
LLRLFREKDRDIPVLMLTAKSQMNDKLLGLQLGADDYVTKPFNYAELILRVKNMTRRVKK
HHHHHHHCCCCCCEEEEECCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHH
KEVTVSHEVIEAGEITICPKERKVHVSEQEIQLTYREFNLCQLFVSNPQRVFMRDELLEK
HHHHHHHHHHCCCCEEECCCCCEEECCHHHEEEEEHHHHEEEEECCCCHHHHHHHHHHHH
VWGFEYIGNTRAVDIMVQRLRKKLGNSGEYIKTIYGVGYKLDC
HCCCHHCCCCHHHHHHHHHHHHHHCCCCCEEHHHHCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1554697 [H]