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The map label for this gene is glcA [H]

Identifier: 49188063

GI number: 49188063

Start: 4954911

End: 4956569

Strand: Reverse

Name: glcA [H]

Synonym: BAS5077

Alternate gene names: 49188063

Gene position: 4956569-4954911 (Counterclockwise)

Preceding gene: 49188065

Following gene: 49188062

Centisome position: 94.8

GC content: 39.24

Gene sequence:

>1659_bases
ATGAACACATGGACACAAGTTTATGATCCGTTCGGAAACATTTGGATTTCGGCAGCGGTCGCACTTATTCCGATTATCTT
TTTTTTCTTAGCTTTAGCAGTTTTCCGCATGAAGGGGTATGTGGCAGGATTTATTACAGTTGTACTGACAATTTTAGTGG
CGTTATTCGCTTATAAGATGCCGTTCACGATGGCGATGGCAGCGACCGGATATGGTTTCTTATACGGATTATGGCCAATT
GCTTGGATTATCATTATGTCGGTATTTTTATATAAAATTTCTGTGAAAACAGGTCAATTCGATGTGATTCGTGCATCTGT
ATTATCCATTACGAATGACCATCGTTTACTCGTTATTTTAATCGGTTTTTCATTCGGAGCATTTTTAGAAGGAGCAGCAG
GATTTGGTGCACCAGTAGCGATTACGGCAGCACTTCTTGCAGGTCTTGGGTTAAATCCTTTATATGCAGCGGGTCTTTGT
TTAATTGCAAATACAGCACCAGTCGCTTTTGGAGCGATGGGAATTCCAATTACAGTTGCTGGACAAGTAACAGGTATTGA
TCCACATAAAATTGGACAAATGGCTGGACATCAATTACCGTTCTTATCATTATTTGTTCCGTTCTTTATCGTATTTTTAA
TGGATGGGTTAAAAGGGGTAAGACAAACATGGCCTGCTTTACTTGTAGCGGGTAGTTCGTTTGCCATTACGCAGTTCATT
ACGGCGACATTCTTAGGGCCAGAACTTCCGGATATTACGTCAGCACTTGTAAGTTTAGTTAGTTTAGCGCTGTTCTTAAA
AGTATGGCAACCGAAAGAAATCTATCAATCAGGGCAAGCAAATAGTGAAGCAGCTGCGACAACAGCGGCATCGATGCCGA
AATTAACGATAGGAAAAGTTGTAAAGGCATGGTCTCCATTTATCGTGTTAACAGTTATGGTAGTCATTTGGAGTCAAAGT
TTCTTTAAAGCATTATTTGCTCCAGGAGGCGCGTTAGAAAGTCTTGTATTTAAGTTTGAAATTCCGGGCTTGCACAATTT
AGTAATGAAAGCAGAGCCGATTGTAAATAAACCGACGCCTTACGAGGCAATTTTGAAGTTCGATGTATTATCAGCAACTG
GAACAGCAATTTTAATTGCTTGTCTCATTTCCATGCTGATTTTGAAAATGAGTGTAAAAGATGCAGCAGTAACATTTAAA
GAAACTTTAAGCGAATTGAAAATGCCAATTCTATCTATTGGATTTGTATTAGGATTTGCATTTATCGCAAACTATTCTGG
ACTATCTTCTACACTAGCATTAGCGTTAGCAGGAACTGGCGGGCTATTCCCGTTCTTCTCACCATTCTTAGGCTGGATTG
GTGTATTCTTAACAGGGTCTGATACGTCAGCAAATGCATTATTCTCAAATTTACAAGCGATTACAGCACAACAAGTCGGT
GTATCTGAAGTACTTCTCGTTGCAGCAAATACAACGGGCGGTGTAACAGGAAAAATGATTTCTCCGCAATCGATTGCGAT
TGCTTGTGCAGCAGTTGGACTTGCTGGAAAAGAATCAGATTTATTCCGCTTTACAGTAAAGCATAGTTTATTTTTCGTAA
TTATTGTTGGTATTATGACGTATGTACAGGCTTATTATTTAACTTGGATGATTCCATAA

Upstream 100 bases:

>100_bases
TTAAACGTTTTTTTATTTTTAGGCTGAAAGGTATGATGACCTGATAGTCTATTGATGAGGTTATTCTATTTAAAAGGGGA
TTCTTTAGGGGGAGAGAACA

Downstream 100 bases:

>100_bases
AGAGCAAAAGAAATAAACTTTTCATTTTTCAGATTATCGTGTATGATGAGAAAGTATTGAAAATTAAATCAGTGATAAAC
TAGGGGTGCCTAACGTATGC

Product: L-lactate permease

Products: lactate [Cytoplasm]; Proton [Cytoplasm]; glycolate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 552; Mature: 552

Protein sequence:

>552_residues
MNTWTQVYDPFGNIWISAAVALIPIIFFFLALAVFRMKGYVAGFITVVLTILVALFAYKMPFTMAMAATGYGFLYGLWPI
AWIIIMSVFLYKISVKTGQFDVIRASVLSITNDHRLLVILIGFSFGAFLEGAAGFGAPVAITAALLAGLGLNPLYAAGLC
LIANTAPVAFGAMGIPITVAGQVTGIDPHKIGQMAGHQLPFLSLFVPFFIVFLMDGLKGVRQTWPALLVAGSSFAITQFI
TATFLGPELPDITSALVSLVSLALFLKVWQPKEIYQSGQANSEAAATTAASMPKLTIGKVVKAWSPFIVLTVMVVIWSQS
FFKALFAPGGALESLVFKFEIPGLHNLVMKAEPIVNKPTPYEAILKFDVLSATGTAILIACLISMLILKMSVKDAAVTFK
ETLSELKMPILSIGFVLGFAFIANYSGLSSTLALALAGTGGLFPFFSPFLGWIGVFLTGSDTSANALFSNLQAITAQQVG
VSEVLLVAANTTGGVTGKMISPQSIAIACAAVGLAGKESDLFRFTVKHSLFFVIIVGIMTYVQAYYLTWMIP

Sequences:

>Translated_552_residues
MNTWTQVYDPFGNIWISAAVALIPIIFFFLALAVFRMKGYVAGFITVVLTILVALFAYKMPFTMAMAATGYGFLYGLWPI
AWIIIMSVFLYKISVKTGQFDVIRASVLSITNDHRLLVILIGFSFGAFLEGAAGFGAPVAITAALLAGLGLNPLYAAGLC
LIANTAPVAFGAMGIPITVAGQVTGIDPHKIGQMAGHQLPFLSLFVPFFIVFLMDGLKGVRQTWPALLVAGSSFAITQFI
TATFLGPELPDITSALVSLVSLALFLKVWQPKEIYQSGQANSEAAATTAASMPKLTIGKVVKAWSPFIVLTVMVVIWSQS
FFKALFAPGGALESLVFKFEIPGLHNLVMKAEPIVNKPTPYEAILKFDVLSATGTAILIACLISMLILKMSVKDAAVTFK
ETLSELKMPILSIGFVLGFAFIANYSGLSSTLALALAGTGGLFPFFSPFLGWIGVFLTGSDTSANALFSNLQAITAQQVG
VSEVLLVAANTTGGVTGKMISPQSIAIACAAVGLAGKESDLFRFTVKHSLFFVIIVGIMTYVQAYYLTWMIP
>Mature_552_residues
MNTWTQVYDPFGNIWISAAVALIPIIFFFLALAVFRMKGYVAGFITVVLTILVALFAYKMPFTMAMAATGYGFLYGLWPI
AWIIIMSVFLYKISVKTGQFDVIRASVLSITNDHRLLVILIGFSFGAFLEGAAGFGAPVAITAALLAGLGLNPLYAAGLC
LIANTAPVAFGAMGIPITVAGQVTGIDPHKIGQMAGHQLPFLSLFVPFFIVFLMDGLKGVRQTWPALLVAGSSFAITQFI
TATFLGPELPDITSALVSLVSLALFLKVWQPKEIYQSGQANSEAAATTAASMPKLTIGKVVKAWSPFIVLTVMVVIWSQS
FFKALFAPGGALESLVFKFEIPGLHNLVMKAEPIVNKPTPYEAILKFDVLSATGTAILIACLISMLILKMSVKDAAVTFK
ETLSELKMPILSIGFVLGFAFIANYSGLSSTLALALAGTGGLFPFFSPFLGWIGVFLTGSDTSANALFSNLQAITAQQVG
VSEVLLVAANTTGGVTGKMISPQSIAIACAAVGLAGKESDLFRFTVKHSLFFVIIVGIMTYVQAYYLTWMIP

Specific function: Transports glycolate across the membrane. Can also transport L-lactate and D-lactate. Seems to be driven by a proton motive force [H]

COG id: COG1620

COG function: function code C; L-lactate permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lactate permease family [H]

Homologues:

Organism=Escherichia coli, GI1789347, Length=558, Percent_Identity=66.8458781362007, Blast_Score=729, Evalue=0.0,
Organism=Escherichia coli, GI1790031, Length=552, Percent_Identity=65.2173913043478, Blast_Score=686, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003804 [H]

Pfam domain/function: PF02652 Lactate_perm [H]

EC number: NA

Molecular weight: Translated: 58847; Mature: 58847

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTWTQVYDPFGNIWISAAVALIPIIFFFLALAVFRMKGYVAGFITVVLTILVALFAYKM
CCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PFTMAMAATGYGFLYGLWPIAWIIIMSVFLYKISVKTGQFDVIRASVLSITNDHRLLVIL
CHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEEEE
IGFSFGAFLEGAAGFGAPVAITAALLAGLGLNPLYAAGLCLIANTAPVAFGAMGIPITVA
EECCHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHEECCCCHHHCCCCCCEEEE
GQVTGIDPHKIGQMAGHQLPFLSLFVPFFIVFLMDGLKGVRQTWPALLVAGSSFAITQFI
CEEECCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHH
TATFLGPELPDITSALVSLVSLALFLKVWQPKEIYQSGQANSEAAATTAASMPKLTIGKV
HHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHCCCCHHHHHH
VKAWSPFIVLTVMVVIWSQSFFKALFAPGGALESLVFKFEIPGLHNLVMKAEPIVNKPTP
HHHHCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHEEEEECCCHHHHHHCCCCCCCCCCC
YEAILKFDVLSATGTAILIACLISMLILKMSVKDAAVTFKETLSELKMPILSIGFVLGFA
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FIANYSGLSSTLALALAGTGGLFPFFSPFLGWIGVFLTGSDTSANALFSNLQAITAQQVG
HHHCCCCHHHHHHHHEECCCCCHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHCC
VSEVLLVAANTTGGVTGKMISPQSIAIACAAVGLAGKESDLFRFTVKHSLFFVIIVGIMT
HHHEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
YVQAYYLTWMIP
HHHHHHHHEECC
>Mature Secondary Structure
MNTWTQVYDPFGNIWISAAVALIPIIFFFLALAVFRMKGYVAGFITVVLTILVALFAYKM
CCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PFTMAMAATGYGFLYGLWPIAWIIIMSVFLYKISVKTGQFDVIRASVLSITNDHRLLVIL
CHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEEEE
IGFSFGAFLEGAAGFGAPVAITAALLAGLGLNPLYAAGLCLIANTAPVAFGAMGIPITVA
EECCHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHEECCCCHHHCCCCCCEEEE
GQVTGIDPHKIGQMAGHQLPFLSLFVPFFIVFLMDGLKGVRQTWPALLVAGSSFAITQFI
CEEECCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHH
TATFLGPELPDITSALVSLVSLALFLKVWQPKEIYQSGQANSEAAATTAASMPKLTIGKV
HHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHCCCCHHHHHH
VKAWSPFIVLTVMVVIWSQSFFKALFAPGGALESLVFKFEIPGLHNLVMKAEPIVNKPTP
HHHHCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHEEEEECCCHHHHHHCCCCCCCCCCC
YEAILKFDVLSATGTAILIACLISMLILKMSVKDAAVTFKETLSELKMPILSIGFVLGFA
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FIANYSGLSSTLALALAGTGGLFPFFSPFLGWIGVFLTGSDTSANALFSNLQAITAQQVG
HHHCCCCHHHHHHHHEECCCCCHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHCC
VSEVLLVAANTTGGVTGKMISPQSIAIACAAVGLAGKESDLFRFTVKHSLFFVIIVGIMT
HHHEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
YVQAYYLTWMIP
HHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: lactate [Periplasm]; Proton [Periplasm]; glycolate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + lactate [Periplasm] = Proton [Cytoplasm] + lactate [Cytoplasm] Proton [Periplasm] + glycolate [Periplasm] = Proton [Cytoplasm] + glycolate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503; 11283302; 11785976 [H]