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The map label for this gene is thiC

Identifier: 49188062

GI number: 49188062

Start: 4952851

End: 4954611

Strand: Reverse

Name: thiC

Synonym: BAS5076

Alternate gene names: 49188062

Gene position: 4954611-4952851 (Counterclockwise)

Preceding gene: 49188063

Following gene: 49188058

Centisome position: 94.76

GC content: 42.76

Gene sequence:

>1761_bases
ATGAAACAGTCTGTTTCAGCTGAGCAAATTGAATTGAAATCGAGTTTACCAGGAAGTAAGAAAGTGTATGTGGATGGACC
ACGAGAAGGTATGAAAGTGCCGATGCGTGAGATTGAACAAAGTGATACAAATGGCGTTCCAAATCCGCCAATTCGTGTGT
ATGATACAAGCGGTCCTTACACAGATCCTGCGTATAAAGTCGAGTTAGAGAAGGGGATTCCAACGCCGCGCCACTCTTGG
ATTCTAGAGCGCGGAGATGTAGAGGCATACGAAGGGCGCGAAGTGAAACCAGAGGATGACGGTGTGAAGGTGGCTTCGAA
ACATACACCTGTTTTCCCGCAAATGGATCGCAAACCGCTTAGAGCGAAGCAAGGTGCAAATGTTACGCAAATGCATTATG
CACGTAATGGCATCATTAAGTCTGAGATGGAATATGTTGCGATTCGTGAAGGAGTAGACCCGGAATTTGTTCGTAAGGAA
ATCGCGGAAGGTCGCGCTATTTTACCAGCGAATATTAACCATCCTGAAGCAGAACCGATGATTATTGGGCGTAATTTCCA
TGTGAAGGTTAATGCGAATATCGGAAACTCTGCTGTATCTTCTTCTATTGCAGAAGAAGTAGAGAAGATGACGTGGGCAA
CTCGCTGGGGTGCAGATACGATTATGGATTTATCTACAGGTAAAAACATTCATACGACGCGCGAGTGGATTATTCGTAAC
GCACCTGTACCAGTTGGAACTGTACCAATCTATCAAGCACTGGAAAAAGTAAACGGAATTGCAGAAGATTTAACGTGGGA
AGTGTATCGTGATACGTTAATTGAGCAAGCGGAGCAAGGCGTAGATTACTTTACGATTCACGCTGGCGTATTACTTCGTT
ACATTCCAATTACGGCGAAACGTACGACAGGTATCGTTTCACGCGGTGGTTCAATTATGGCACAGTGGTGTTTATTCCAT
CATAAAGAAAACTTCCTATACACTCATTTTGAAGAGATTTGTGAAATTATGAAGCAGTACGATGTTTCGTTCTCTCTTGG
AGATGGATTACGTCCAGGTTCGATTGCAGATGCAAATGACGAAGCACAGTTTTCTGAGCTTGAAACACTTGGTGAATTAA
CGAAGATTGCTTGGAAACATGATGTGCAAGTGATGATTGAAGGGCCTGGGCATGTACCGATGCATTTAATTAAAGAGAAT
ATGGAGAAAGAACTTGATATTTGTCAGGGCGCGCCGTTCTATACACTTGGGCCGTTAACGACAGATATTGCACCAGGTTA
TGACCATATTACATCTGCGATTGGAGCTGCGATGATTGGTTGGTTTGGAACGGCGATGCTTTGTTATGTAACGCCGAAAG
AACATTTAGGTTTACCAAATAAAGATGATGTTCGAGAAGGTGTTATTACGTACAAAATCGCTGCACATGCGGCTGATCTA
GCGAAAGGTCACAAAACGGCTCATCAGCGTGATGATGCCCTTTCAAAAGCACGCTTTGAATTCCGTTGGCGCGATCAATT
TAATTTATCTTTAGATCCTGAACGCGCGATGGAGTATCACGATGAAACATTGCCAGCAGAAGGAGCGAAAACGGCTCATT
TCTGTTCCATGTGTGGACCGAAGTTTTGTAGTATGAGAATTTCACATGATATTCGTGAATACGCAAAAGAAAATGATTTA
GAAACGACAGAAGCAATTGAAAAAGGAATGAAAGAGAAAGCGAAAGAATTTAAAGAAACTGGTAGTCATTTATACCAATA
A

Upstream 100 bases:

>100_bases
ATGAGCCAGGTCCTTATTTTTTTATGGACCTGGCTCTTTTTATTTTGGCATACGCTGGCCATATCTCGTCCTTGTCACGT
ACAGGGGGGCAACAAAGGTT

Downstream 100 bases:

>100_bases
TACGTTACACCTCTTTACTTGATTGATCATACTGTAGTACCTAACTTAAATATAGAAGAAGCCCGCTTTGCTAAGACGGT
AGCAAAGTGGGCTTCTGTCG

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC

Number of amino acids: Translated: 586; Mature: 586

Protein sequence:

>586_residues
MKQSVSAEQIELKSSLPGSKKVYVDGPREGMKVPMREIEQSDTNGVPNPPIRVYDTSGPYTDPAYKVELEKGIPTPRHSW
ILERGDVEAYEGREVKPEDDGVKVASKHTPVFPQMDRKPLRAKQGANVTQMHYARNGIIKSEMEYVAIREGVDPEFVRKE
IAEGRAILPANINHPEAEPMIIGRNFHVKVNANIGNSAVSSSIAEEVEKMTWATRWGADTIMDLSTGKNIHTTREWIIRN
APVPVGTVPIYQALEKVNGIAEDLTWEVYRDTLIEQAEQGVDYFTIHAGVLLRYIPITAKRTTGIVSRGGSIMAQWCLFH
HKENFLYTHFEEICEIMKQYDVSFSLGDGLRPGSIADANDEAQFSELETLGELTKIAWKHDVQVMIEGPGHVPMHLIKEN
MEKELDICQGAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWFGTAMLCYVTPKEHLGLPNKDDVREGVITYKIAAHAADL
AKGHKTAHQRDDALSKARFEFRWRDQFNLSLDPERAMEYHDETLPAEGAKTAHFCSMCGPKFCSMRISHDIREYAKENDL
ETTEAIEKGMKEKAKEFKETGSHLYQ

Sequences:

>Translated_586_residues
MKQSVSAEQIELKSSLPGSKKVYVDGPREGMKVPMREIEQSDTNGVPNPPIRVYDTSGPYTDPAYKVELEKGIPTPRHSW
ILERGDVEAYEGREVKPEDDGVKVASKHTPVFPQMDRKPLRAKQGANVTQMHYARNGIIKSEMEYVAIREGVDPEFVRKE
IAEGRAILPANINHPEAEPMIIGRNFHVKVNANIGNSAVSSSIAEEVEKMTWATRWGADTIMDLSTGKNIHTTREWIIRN
APVPVGTVPIYQALEKVNGIAEDLTWEVYRDTLIEQAEQGVDYFTIHAGVLLRYIPITAKRTTGIVSRGGSIMAQWCLFH
HKENFLYTHFEEICEIMKQYDVSFSLGDGLRPGSIADANDEAQFSELETLGELTKIAWKHDVQVMIEGPGHVPMHLIKEN
MEKELDICQGAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWFGTAMLCYVTPKEHLGLPNKDDVREGVITYKIAAHAADL
AKGHKTAHQRDDALSKARFEFRWRDQFNLSLDPERAMEYHDETLPAEGAKTAHFCSMCGPKFCSMRISHDIREYAKENDL
ETTEAIEKGMKEKAKEFKETGSHLYQ
>Mature_586_residues
MKQSVSAEQIELKSSLPGSKKVYVDGPREGMKVPMREIEQSDTNGVPNPPIRVYDTSGPYTDPAYKVELEKGIPTPRHSW
ILERGDVEAYEGREVKPEDDGVKVASKHTPVFPQMDRKPLRAKQGANVTQMHYARNGIIKSEMEYVAIREGVDPEFVRKE
IAEGRAILPANINHPEAEPMIIGRNFHVKVNANIGNSAVSSSIAEEVEKMTWATRWGADTIMDLSTGKNIHTTREWIIRN
APVPVGTVPIYQALEKVNGIAEDLTWEVYRDTLIEQAEQGVDYFTIHAGVLLRYIPITAKRTTGIVSRGGSIMAQWCLFH
HKENFLYTHFEEICEIMKQYDVSFSLGDGLRPGSIADANDEAQFSELETLGELTKIAWKHDVQVMIEGPGHVPMHLIKEN
MEKELDICQGAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWFGTAMLCYVTPKEHLGLPNKDDVREGVITYKIAAHAADL
AKGHKTAHQRDDALSKARFEFRWRDQFNLSLDPERAMEYHDETLPAEGAKTAHFCSMCGPKFCSMRISHDIREYAKENDL
ETTEAIEKGMKEKAKEFKETGSHLYQ

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=613, Percent_Identity=64.4371941272431, Blast_Score=806, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC_BACAA (C3P178)

Other databases:

- EMBL:   CP001598
- RefSeq:   YP_002869444.1
- EnsemblBacteria:   EBBACT00000129784
- GeneID:   7851009
- GenomeReviews:   CP001598_GR
- KEGG:   bai:BAA_5491
- GeneTree:   EBGT00050000001472
- ProtClustDB:   PRK09284
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 65798; Mature: 65798

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: NA

Important sites: BINDING 193-193 BINDING 222-222 BINDING 251-251 BINDING 287-287 BINDING 387-387 BINDING 414-414

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQSVSAEQIELKSSLPGSKKVYVDGPREGMKVPMREIEQSDTNGVPNPPIRVYDTSGPY
CCCCCCHHHHHHHHCCCCCCEEEECCCCCCCCCCHHHHHCCCCCCCCCCCEEEEECCCCC
TDPAYKVELEKGIPTPRHSWILERGDVEAYEGREVKPEDDGVKVASKHTPVFPQMDRKPL
CCCCEEEEECCCCCCCCHHHEEECCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCH
RAKQGANVTQMHYARNGIIKSEMEYVAIREGVDPEFVRKEIAEGRAILPANINHPEAEPM
HHHCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEECCCCCCCCCCE
IIGRNFHVKVNANIGNSAVSSSIAEEVEKMTWATRWGADTIMDLSTGKNIHTTREWIIRN
EECCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHCC
APVPVGTVPIYQALEKVNGIAEDLTWEVYRDTLIEQAEQGVDYFTIHAGVLLRYIPITAK
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCCC
RTTGIVSRGGSIMAQWCLFHHKENFLYTHFEEICEIMKQYDVSFSLGDGLRPGSIADAND
HHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCC
EAQFSELETLGELTKIAWKHDVQVMIEGPGHVPMHLIKENMEKELDICQGAPFYTLGPLT
CHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCC
TDIAPGYDHITSAIGAAMIGWFGTAMLCYVTPKEHLGLPNKDDVREGVITYKIAAHAADL
CCCCCCHHHHHHHHHHHHHHHHHHEEEEEECCHHHCCCCCHHHHHCCCEEEEHHHHHHHH
AKGHKTAHQRDDALSKARFEFRWRDQFNLSLDPERAMEYHDETLPAEGAKTAHFCSMCGP
HHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHCCH
KFCSMRISHDIREYAKENDLETTEAIEKGMKEKAKEFKETGSHLYQ
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKQSVSAEQIELKSSLPGSKKVYVDGPREGMKVPMREIEQSDTNGVPNPPIRVYDTSGPY
CCCCCCHHHHHHHHCCCCCCEEEECCCCCCCCCCHHHHHCCCCCCCCCCCEEEEECCCCC
TDPAYKVELEKGIPTPRHSWILERGDVEAYEGREVKPEDDGVKVASKHTPVFPQMDRKPL
CCCCEEEEECCCCCCCCHHHEEECCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCH
RAKQGANVTQMHYARNGIIKSEMEYVAIREGVDPEFVRKEIAEGRAILPANINHPEAEPM
HHHCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEECCCCCCCCCCE
IIGRNFHVKVNANIGNSAVSSSIAEEVEKMTWATRWGADTIMDLSTGKNIHTTREWIIRN
EECCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHCC
APVPVGTVPIYQALEKVNGIAEDLTWEVYRDTLIEQAEQGVDYFTIHAGVLLRYIPITAK
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCCC
RTTGIVSRGGSIMAQWCLFHHKENFLYTHFEEICEIMKQYDVSFSLGDGLRPGSIADAND
HHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCC
EAQFSELETLGELTKIAWKHDVQVMIEGPGHVPMHLIKENMEKELDICQGAPFYTLGPLT
CHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCC
TDIAPGYDHITSAIGAAMIGWFGTAMLCYVTPKEHLGLPNKDDVREGVITYKIAAHAADL
CCCCCCHHHHHHHHHHHHHHHHHHEEEEEECCHHHCCCCCHHHHHCCCEEEEHHHHHHHH
AKGHKTAHQRDDALSKARFEFRWRDQFNLSLDPERAMEYHDETLPAEGAKTAHFCSMCGP
HHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHCCH
KFCSMRISHDIREYAKENDLETTEAIEKGMKEKAKEFKETGSHLYQ
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA