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The map label for this gene is tpiA

Identifier: 49187973

GI number: 49187973

Start: 4862573

End: 4863328

Strand: Reverse

Name: tpiA

Synonym: BAS4987

Alternate gene names: 49187973

Gene position: 4863328-4862573 (Counterclockwise)

Preceding gene: 49187974

Following gene: 49187972

Centisome position: 93.01

GC content: 43.78

Gene sequence:

>756_bases
ATGCGTAAACCAATTATCGCAGGTAACTGGAAAATGAATAAAACTCTATCTGAAGCAGTTAGCTTCGTAGAGGAAGTTAA
AGGTCAAATCCCAGCAGCTTCAGCTGTTGATGCAGTAGTTTGCTCTCCAGCTCTATTCTTAGAGCGCCTGGTAGCAGCGA
CTGAAGGAACTGATTTACAAGTAGGTGCACAAAACATGCACTTCGAAAAAAATGGTGCATTCACTGGCGAAATTAGCCCA
GTAGCACTTAGCGACTTAAAAGTAGGCTACGTAGTACTTGGCCACTCTGAGCGTCGTGAAATGTTTGCTGAAACAGATGA
GTCAGTAAACAAAAAGACTATTGCAGCATTTGAACATGGTTTAACACCAATCGTATGTTGTGGTGAGACTTTAGAAGAGC
GCGAAAGCGGAAAAACATTTGATCTAGTAGCAGGTCAAGTGACAAAAGCACTTGCAGGTTTAACAGAAGAGCAAGTTAAA
GCAACTGTTATCGCTTATGAGCCAATCTGGGCTATCGGTACAGGTAAATCTTCTTCTTCTGCAGATGCAAACGAAGTATG
TGCGCACATCCGTAAAGTTGTTGCAGAAGTTGTTTCTCCAGCTGCTGCAGAAGCTGTTCGTATCCAATACGGCGGTAGCG
TAAAACCAGAAAACATTAAAGAGTACATGGCACAATCTGACATCGACGGCGCTTTAGTTGGCGGTGCTAGCTTAGAGCCT
GCTTCGTTCTTAGGTCTTCTGGGGGCGGTAAAATGA

Upstream 100 bases:

>100_bases
GCGGTGCGTCATTAGAATTCATGGAAGGTAAAGAGCTTCCAGGTGTAGTTTGTCTTAACGACAAATAAGTAGCAGCCAAA
GAAAAAGGACGGTGCAAAGC

Downstream 100 bases:

>100_bases
GAAAGCCAACAGCTTTAATCATTCTTGACGGTTTCGGACTTCGTGAAGAAACTTACGGGAATGCTGTAGCACAAGCTAAG
AAACCTAATTTTGATGGTTA

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MRKPIIAGNWKMNKTLSEAVSFVEEVKGQIPAASAVDAVVCSPALFLERLVAATEGTDLQVGAQNMHFEKNGAFTGEISP
VALSDLKVGYVVLGHSERREMFAETDESVNKKTIAAFEHGLTPIVCCGETLEERESGKTFDLVAGQVTKALAGLTEEQVK
ATVIAYEPIWAIGTGKSSSSADANEVCAHIRKVVAEVVSPAAAEAVRIQYGGSVKPENIKEYMAQSDIDGALVGGASLEP
ASFLGLLGAVK

Sequences:

>Translated_251_residues
MRKPIIAGNWKMNKTLSEAVSFVEEVKGQIPAASAVDAVVCSPALFLERLVAATEGTDLQVGAQNMHFEKNGAFTGEISP
VALSDLKVGYVVLGHSERREMFAETDESVNKKTIAAFEHGLTPIVCCGETLEERESGKTFDLVAGQVTKALAGLTEEQVK
ATVIAYEPIWAIGTGKSSSSADANEVCAHIRKVVAEVVSPAAAEAVRIQYGGSVKPENIKEYMAQSDIDGALVGGASLEP
ASFLGLLGAVK
>Mature_251_residues
MRKPIIAGNWKMNKTLSEAVSFVEEVKGQIPAASAVDAVVCSPALFLERLVAATEGTDLQVGAQNMHFEKNGAFTGEISP
VALSDLKVGYVVLGHSERREMFAETDESVNKKTIAAFEHGLTPIVCCGETLEERESGKTFDLVAGQVTKALAGLTEEQVK
ATVIAYEPIWAIGTGKSSSSADANEVCAHIRKVVAEVVSPAAAEAVRIQYGGSVKPENIKEYMAQSDIDGALVGGASLEP
ASFLGLLGAVK

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI4507645, Length=251, Percent_Identity=43.0278884462151, Blast_Score=176, Evalue=2e-44,
Organism=Homo sapiens, GI226529917, Length=251, Percent_Identity=43.0278884462151, Blast_Score=176, Evalue=2e-44,
Organism=Escherichia coli, GI1790353, Length=243, Percent_Identity=41.9753086419753, Blast_Score=194, Evalue=6e-51,
Organism=Caenorhabditis elegans, GI17536593, Length=249, Percent_Identity=44.9799196787149, Blast_Score=188, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6320255, Length=243, Percent_Identity=41.1522633744856, Blast_Score=177, Evalue=1e-45,
Organism=Drosophila melanogaster, GI28572008, Length=252, Percent_Identity=42.4603174603175, Blast_Score=176, Evalue=2e-44,
Organism=Drosophila melanogaster, GI28572006, Length=252, Percent_Identity=42.4603174603175, Blast_Score=176, Evalue=2e-44,
Organism=Drosophila melanogaster, GI28572004, Length=252, Percent_Identity=42.4603174603175, Blast_Score=175, Evalue=2e-44,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_BACAN (Q81X76)

Other databases:

- EMBL:   AE016879
- EMBL:   AE017334
- EMBL:   AE017225
- RefSeq:   NP_847540.1
- RefSeq:   YP_022025.2
- RefSeq:   YP_031226.1
- ProteinModelPortal:   Q81X76
- SMR:   Q81X76
- EnsemblBacteria:   EBBACT00000009724
- EnsemblBacteria:   EBBACT00000018765
- EnsemblBacteria:   EBBACT00000020325
- GeneID:   1084904
- GeneID:   2818888
- GeneID:   2851859
- GenomeReviews:   AE016879_GR
- GenomeReviews:   AE017225_GR
- GenomeReviews:   AE017334_GR
- KEGG:   ban:BA_5366
- KEGG:   bar:GBAA_5366
- KEGG:   bat:BAS4987
- TIGR:   BA_5366
- TIGR:   GBAA_5366
- GeneTree:   EBGT00050000002739
- HOGENOM:   HBG708281
- OMA:   DIRSVQT
- ProtClustDB:   PRK00042
- BioCyc:   BANT260799:BAS4987-MONOMER
- BioCyc:   BANT261594:GBAA5366-MONOMER
- BRENDA:   5.3.1.1
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 26438; Mature: 26438

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 95-95 ACT_SITE 167-167 BINDING 9-9 BINDING 11-11

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKPIIAGNWKMNKTLSEAVSFVEEVKGQIPAASAVDAVVCSPALFLERLVAATEGTDLQ
CCCCEECCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEE
VGAQNMHFEKNGAFTGEISPVALSDLKVGYVVLGHSERREMFAETDESVNKKTIAAFEHG
ECCCCCEEECCCCCCCCCCCEEECCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHC
LTPIVCCGETLEERESGKTFDLVAGQVTKALAGLTEEQVKATVIAYEPIWAIGTGKSSSS
CCEEEECCHHHHHHHCCCEEEHHHHHHHHHHHCCCHHHHEEEEEEECCEEEEECCCCCCC
ADANEVCAHIRKVVAEVVSPAAAEAVRIQYGGSVKPENIKEYMAQSDIDGALVGGASLEP
CCHHHHHHHHHHHHHHHHCCHHHHEEEEEECCCCCHHHHHHHHHHCCCCCEEECCCCCCH
ASFLGLLGAVK
HHHHHHHHCCC
>Mature Secondary Structure
MRKPIIAGNWKMNKTLSEAVSFVEEVKGQIPAASAVDAVVCSPALFLERLVAATEGTDLQ
CCCCEECCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEE
VGAQNMHFEKNGAFTGEISPVALSDLKVGYVVLGHSERREMFAETDESVNKKTIAAFEHG
ECCCCCEEECCCCCCCCCCCEEECCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHC
LTPIVCCGETLEERESGKTFDLVAGQVTKALAGLTEEQVKATVIAYEPIWAIGTGKSSSS
CCEEEECCHHHHHHHCCCEEEHHHHHHHHHHHCCCHHHHEEEEEEECCEEEEECCCCCCC
ADANEVCAHIRKVVAEVVSPAAAEAVRIQYGGSVKPENIKEYMAQSDIDGALVGGASLEP
CCHHHHHHHHHHHHHHHHCCHHHHEEEEEECCCCCHHHHHHHHHHCCCCCEEECCCCCCH
ASFLGLLGAVK
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629