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The map label for this gene is pgk

Identifier: 49187974

GI number: 49187974

Start: 4863361

End: 4864545

Strand: Reverse

Name: pgk

Synonym: BAS4988

Alternate gene names: 49187974

Gene position: 4864545-4863361 (Counterclockwise)

Preceding gene: 49187975

Following gene: 49187973

Centisome position: 93.04

GC content: 41.18

Gene sequence:

>1185_bases
ATGAACAAAAAATCAATTCGTGACGTAGATTTAAAAGGTAAGCGTGTATTTTGTCGCGTTGACTTCAACGTACCTATGAA
AGAAGGCAAAATTACAGATGAAACTCGTATCCGTGCAGCTCTTCCTACAATTCAATATTTAGTAGAGCAAGGTGCGAAGG
TTATTTTAGCAAGCCACTTAGGCCGTCCAAAAGGTCAAGCAGTAGAAGAATTACGTCTTACTCCAGTAGCAGCACGTTTA
GGCGAGCTTCTTGGTAAAGATGTTAAAAAAGCAGACGAAGCATTCGGACCAGTTGCACAAGAAATGGTTGCAGCAATGAA
CGAAGGCGACGTATTAGTTCTTGAAAACGTACGTTTCTATGCGGGCGAAGAAAAGAACGATGCAGAACTTGCGAAAGAAT
TTGCAGCTCTTGCTGACATCTTCGTAAACGATGCATTCGGTGCAGCTCACCGTGCTCATGCTTCTACAGCAGGAATCGCA
GACTACCTACCAGCAGTATCTGGTCTATTAATGGAAAAAGAGTTAGAAGTATTAGGTAAAGCACTTTCTAACCCAGAACG
TCCATTCACAGCTATCATCGGTGGTGCGAAAGTAAAAGATAAAATCGGTTTAATTCGTCATCTATTAGACAAAGTAGATA
ACTTAATCATCGGTGGCGGACTTGCTTACACATTCGTTAAAGCTTTAGGTCATGAAATTGGTCTATCTCTATGTGAAGAC
GACAAGATTGAACTAGCTAAAGAATTTATGCAACTTGCAAAAGAAAAAGGCGTAAACTTCTACATGCCAGTTGATGTTGT
AATCACTGAGGAATTCTCTGAAACTGCAACAACTAAAATCGTTGGTATCGACTCTATCCCTTCTAACTGGGAAGGCGTGG
ACATCGGTCCTAAAACACGTGAAATTTATGCAGATGTAATTAAAAACTCTAAGCTTGTTGTATGGAATGGACCAATGGGT
GTATTCGAAATGACTCCATTCGCAGAAGGTACAAAAGCAGTAGGACAAGCATTAGCAGATGCAGAAGGTACATACTCTGT
TATCGGCGGTGGTGACTCTGCAGCAGCTGTTGAAAAATTCGGTATGGCTGATAAAATGAGCCACATTTCTACTGGCGGCG
GTGCGTCATTAGAATTCATGGAAGGTAAAGAGCTTCCAGGTGTAGTTTGTCTTAACGACAAATAA

Upstream 100 bases:

>100_bases
AGGGTTTGTTCCCTCTCTCTCTTCTTTCGTTTTAAAAGCAAATGTGTTAAGCTTTTGAGTGGGTTTTGTCAATCCTAACT
AGTAGTCGGAGGGAAATCCA

Downstream 100 bases:

>100_bases
GTAGCAGCCAAAGAAAAAGGACGGTGCAAAGCATGCGTAAACCAATTATCGCAGGTAACTGGAAAATGAATAAAACTCTA
TCTGAAGCAGTTAGCTTCGT

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MNKKSIRDVDLKGKRVFCRVDFNVPMKEGKITDETRIRAALPTIQYLVEQGAKVILASHLGRPKGQAVEELRLTPVAARL
GELLGKDVKKADEAFGPVAQEMVAAMNEGDVLVLENVRFYAGEEKNDAELAKEFAALADIFVNDAFGAAHRAHASTAGIA
DYLPAVSGLLMEKELEVLGKALSNPERPFTAIIGGAKVKDKIGLIRHLLDKVDNLIIGGGLAYTFVKALGHEIGLSLCED
DKIELAKEFMQLAKEKGVNFYMPVDVVITEEFSETATTKIVGIDSIPSNWEGVDIGPKTREIYADVIKNSKLVVWNGPMG
VFEMTPFAEGTKAVGQALADAEGTYSVIGGGDSAAAVEKFGMADKMSHISTGGGASLEFMEGKELPGVVCLNDK

Sequences:

>Translated_394_residues
MNKKSIRDVDLKGKRVFCRVDFNVPMKEGKITDETRIRAALPTIQYLVEQGAKVILASHLGRPKGQAVEELRLTPVAARL
GELLGKDVKKADEAFGPVAQEMVAAMNEGDVLVLENVRFYAGEEKNDAELAKEFAALADIFVNDAFGAAHRAHASTAGIA
DYLPAVSGLLMEKELEVLGKALSNPERPFTAIIGGAKVKDKIGLIRHLLDKVDNLIIGGGLAYTFVKALGHEIGLSLCED
DKIELAKEFMQLAKEKGVNFYMPVDVVITEEFSETATTKIVGIDSIPSNWEGVDIGPKTREIYADVIKNSKLVVWNGPMG
VFEMTPFAEGTKAVGQALADAEGTYSVIGGGDSAAAVEKFGMADKMSHISTGGGASLEFMEGKELPGVVCLNDK
>Mature_394_residues
MNKKSIRDVDLKGKRVFCRVDFNVPMKEGKITDETRIRAALPTIQYLVEQGAKVILASHLGRPKGQAVEELRLTPVAARL
GELLGKDVKKADEAFGPVAQEMVAAMNEGDVLVLENVRFYAGEEKNDAELAKEFAALADIFVNDAFGAAHRAHASTAGIA
DYLPAVSGLLMEKELEVLGKALSNPERPFTAIIGGAKVKDKIGLIRHLLDKVDNLIIGGGLAYTFVKALGHEIGLSLCED
DKIELAKEFMQLAKEKGVNFYMPVDVVITEEFSETATTKIVGIDSIPSNWEGVDIGPKTREIYADVIKNSKLVVWNGPMG
VFEMTPFAEGTKAVGQALADAEGTYSVIGGGDSAAAVEKFGMADKMSHISTGGGASLEFMEGKELPGVVCLNDK

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI4505763, Length=414, Percent_Identity=47.3429951690821, Blast_Score=355, Evalue=4e-98,
Organism=Homo sapiens, GI31543397, Length=413, Percent_Identity=46.7312348668281, Blast_Score=347, Evalue=2e-95,
Organism=Escherichia coli, GI1789294, Length=396, Percent_Identity=46.4646464646465, Blast_Score=345, Evalue=4e-96,
Organism=Caenorhabditis elegans, GI17508823, Length=412, Percent_Identity=48.3009708737864, Blast_Score=365, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6319857, Length=412, Percent_Identity=49.5145631067961, Blast_Score=377, Evalue=1e-105,
Organism=Drosophila melanogaster, GI17136394, Length=411, Percent_Identity=47.4452554744526, Blast_Score=342, Evalue=3e-94,
Organism=Drosophila melanogaster, GI24581203, Length=415, Percent_Identity=45.5421686746988, Blast_Score=332, Evalue=4e-91,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_BACAA (C3P0A6)

Other databases:

- EMBL:   CP001598
- RefSeq:   YP_002869358.1
- ProteinModelPortal:   C3P0A6
- SMR:   C3P0A6
- EnsemblBacteria:   EBBACT00000128237
- GeneID:   7849170
- GenomeReviews:   CP001598_GR
- KEGG:   bai:BAA_5397
- GeneTree:   EBGT00050000001506
- ProtClustDB:   PRK00073
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 42298; Mature: 42298

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 36-36 BINDING 118-118 BINDING 151-151 BINDING 201-201 BINDING 292-292 BINDING 323-323

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKSIRDVDLKGKRVFCRVDFNVPMKEGKITDETRIRAALPTIQYLVEQGAKVILASHL
CCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEHHHC
GRPKGQAVEELRLTPVAARLGELLGKDVKKADEAFGPVAQEMVAAMNEGDVLVLENVRFY
CCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCEEEEECEEEE
AGEEKNDAELAKEFAALADIFVNDAFGAAHRAHASTAGIADYLPAVSGLLMEKELEVLGK
CCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
ALSNPERPFTAIIGGAKVKDKIGLIRHLLDKVDNLIIGGGLAYTFVKALGHEIGLSLCED
HHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCEEECCHHHHHHHHHHHHHHCCCCCCC
DKIELAKEFMQLAKEKGVNFYMPVDVVITEEFSETATTKIVGIDSIPSNWEGVDIGPKTR
CHHHHHHHHHHHHHHCCCEEEEEEEEEEECCHHCCHHEEEEEECCCCCCCCCCCCCCCHH
EIYADVIKNSKLVVWNGPMGVFEMTPFAEGTKAVGQALADAEGTYSVIGGGDSAAAVEKF
HHHHHHHCCCEEEEEECCCCCEEECCCCCHHHHHHHHHHCCCCCEEEEECCCHHHHHHHH
GMADKMSHISTGGGASLEFMEGKELPGVVCLNDK
CCHHHHHHCCCCCCCEEEEECCCCCCCEEEECCC
>Mature Secondary Structure
MNKKSIRDVDLKGKRVFCRVDFNVPMKEGKITDETRIRAALPTIQYLVEQGAKVILASHL
CCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEHHHC
GRPKGQAVEELRLTPVAARLGELLGKDVKKADEAFGPVAQEMVAAMNEGDVLVLENVRFY
CCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCEEEEECEEEE
AGEEKNDAELAKEFAALADIFVNDAFGAAHRAHASTAGIADYLPAVSGLLMEKELEVLGK
CCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
ALSNPERPFTAIIGGAKVKDKIGLIRHLLDKVDNLIIGGGLAYTFVKALGHEIGLSLCED
HHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCEEECCHHHHHHHHHHHHHHCCCCCCC
DKIELAKEFMQLAKEKGVNFYMPVDVVITEEFSETATTKIVGIDSIPSNWEGVDIGPKTR
CHHHHHHHHHHHHHHCCCEEEEEEEEEEECCHHCCHHEEEEEECCCCCCCCCCCCCCCHH
EIYADVIKNSKLVVWNGPMGVFEMTPFAEGTKAVGQALADAEGTYSVIGGGDSAAAVEKF
HHHHHHHCCCEEEEEECCCCCEEECCCCCHHHHHHHHHHCCCCCEEEEECCCHHHHHHHH
GMADKMSHISTGGGASLEFMEGKELPGVVCLNDK
CCHHHHHHCCCCCCCEEEEECCCCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA