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The map label for this gene is vanSB [H]

Identifier: 49187889

GI number: 49187889

Start: 4781135

End: 4782577

Strand: Direct

Name: vanSB [H]

Synonym: BAS4901

Alternate gene names: 49187889

Gene position: 4781135-4782577 (Clockwise)

Preceding gene: 49187888

Following gene: 49187890

Centisome position: 91.44

GC content: 32.36

Gene sequence:

>1443_bases
TTGGCTACAAAATCGAATCGTAGCAAAGACAACATCACCAAAAATATTTTCATCAAAACCTTATTATTTTGTATTCTTTT
ATCACTTTGTCTTTATCAAATTATTTACTTCTTAGCTTCAGACTACGATAAAGAACGTTTTGCGAAAGAAAATGGACCGT
CTACTACAGAACAAGCGGATTCAGATAAACAAAACGCCCAAAGTAAAACGAATCAAAATAAAGCTGTATCACAGGGAGAC
ATGTTTAATTTTCAATCTTCTATTATAGATTATAACGCTCTCTCTACAGCTATCAATCATCTTTTGCAGCCTAGCCAAAC
TGCAAAAGCAAAAGAACATACTCAAAATATTTCGGGGAAAAATAGCGCTTCTGCTCCATCTACAGAAACGGAAAAGCAAG
TCGCTACTAATGATGCCTTACATAAACAAAATGCAGCAAGTAAAACAAATGATGCACAAGATGATCCAAAATTAACAGAT
GCACTGCAGCAGTTAGCTCCACATATTGGCGCTACAATGCTTGCATTATCTTTGCTCGGATCTTTAATTTATGCAAAAAT
AATTGCTAAACCTTTTCAATATATGAGTGATGCTTTAAAAGATATTATGAATCTTGATTTCTCAGATAAAAAGCTACTTA
ACAAAAATACTGATCAAACAGATTTTAATTTACAAGTAGCTGCTTCACAAGTACCAAATATAGTGAAGAATTTACATGCA
AGCAATCAAGATTTAAGAAATGAACTCAAAAAGGAGCAACAATTAGAACAATCTCGTAAAGAATTTATGTCTATGGTTTC
CCATGAATTAAAAACACCAATCGCAGCCGTTATGGGACAACTAGATGGTATGATTCACGGAATTGGAGCTTATAAAGATC
GAGATAAATATTTAAAACGTTCCTATGAAATGATGCAAGACATTAACATATTAACGGAAAAAATGTCGGAATTATCCAAA
ATACAAAACCCTCAATTTAAACCTGATTTAAAAGTTATCTCATTGACTAATATTATTGAGGATGTTATGAAGAAAGTAGA
TTATTTCGTATCAGTCAAGCAGTTAAATGTTCAATCTAACATTAAACAAGATGTACAAGTTTTAGCTGATCCTAAATTTA
TTCAAACTGCCATTTTTAACATTATTTCAAATGCAATTCATTATACAATTGACCATCAGCATGTATATATAAAGCTTTAT
GAAAAGCCAAATGGTTACGCACTAGAAGTTTTAAATACAGGTTCACAAATAGATGAAGATAAACTTGCCCATTTATTTGA
GCCATTCTATCGCGCAAACCCTGACAAACACGGCTTAGTACAAGGCAGTGGTCTTGGGTTATACATTGTAAAACAAATAC
TAGATAAACATCAATTTCCTTATGGGATACAAAACACGCCTCAAGGTGTAAAATGTTCTATCGTATTCCCAAAAGCAATG
TAA

Upstream 100 bases:

>100_bases
TGGGGCTATGAACATTATGGTGATGTAAGAGTTATAGATACACATATTAAAAACTTACGAAAAAAATTAGGAATTCCTTA
TATTAAAACGGTGAAAGGCA

Downstream 100 bases:

>100_bases
AAACCTACTACAATCTCATACCCCAAATTCATACTTCGTATGAGTGGCGGAATTTTGTAGAGTATATTACTCTGTTTCGT
TCGCCCGTTATCCTTATTTG

Product: sensor histidine kinase

Products: NA

Alternate protein names: Vancomycin B-type resistance protein vanSB; Vancomycin histidine protein kinase [H]

Number of amino acids: Translated: 480; Mature: 479

Protein sequence:

>480_residues
MATKSNRSKDNITKNIFIKTLLFCILLSLCLYQIIYFLASDYDKERFAKENGPSTTEQADSDKQNAQSKTNQNKAVSQGD
MFNFQSSIIDYNALSTAINHLLQPSQTAKAKEHTQNISGKNSASAPSTETEKQVATNDALHKQNAASKTNDAQDDPKLTD
ALQQLAPHIGATMLALSLLGSLIYAKIIAKPFQYMSDALKDIMNLDFSDKKLLNKNTDQTDFNLQVAASQVPNIVKNLHA
SNQDLRNELKKEQQLEQSRKEFMSMVSHELKTPIAAVMGQLDGMIHGIGAYKDRDKYLKRSYEMMQDINILTEKMSELSK
IQNPQFKPDLKVISLTNIIEDVMKKVDYFVSVKQLNVQSNIKQDVQVLADPKFIQTAIFNIISNAIHYTIDHQHVYIKLY
EKPNGYALEVLNTGSQIDEDKLAHLFEPFYRANPDKHGLVQGSGLGLYIVKQILDKHQFPYGIQNTPQGVKCSIVFPKAM

Sequences:

>Translated_480_residues
MATKSNRSKDNITKNIFIKTLLFCILLSLCLYQIIYFLASDYDKERFAKENGPSTTEQADSDKQNAQSKTNQNKAVSQGD
MFNFQSSIIDYNALSTAINHLLQPSQTAKAKEHTQNISGKNSASAPSTETEKQVATNDALHKQNAASKTNDAQDDPKLTD
ALQQLAPHIGATMLALSLLGSLIYAKIIAKPFQYMSDALKDIMNLDFSDKKLLNKNTDQTDFNLQVAASQVPNIVKNLHA
SNQDLRNELKKEQQLEQSRKEFMSMVSHELKTPIAAVMGQLDGMIHGIGAYKDRDKYLKRSYEMMQDINILTEKMSELSK
IQNPQFKPDLKVISLTNIIEDVMKKVDYFVSVKQLNVQSNIKQDVQVLADPKFIQTAIFNIISNAIHYTIDHQHVYIKLY
EKPNGYALEVLNTGSQIDEDKLAHLFEPFYRANPDKHGLVQGSGLGLYIVKQILDKHQFPYGIQNTPQGVKCSIVFPKAM
>Mature_479_residues
ATKSNRSKDNITKNIFIKTLLFCILLSLCLYQIIYFLASDYDKERFAKENGPSTTEQADSDKQNAQSKTNQNKAVSQGDM
FNFQSSIIDYNALSTAINHLLQPSQTAKAKEHTQNISGKNSASAPSTETEKQVATNDALHKQNAASKTNDAQDDPKLTDA
LQQLAPHIGATMLALSLLGSLIYAKIIAKPFQYMSDALKDIMNLDFSDKKLLNKNTDQTDFNLQVAASQVPNIVKNLHAS
NQDLRNELKKEQQLEQSRKEFMSMVSHELKTPIAAVMGQLDGMIHGIGAYKDRDKYLKRSYEMMQDINILTEKMSELSKI
QNPQFKPDLKVISLTNIIEDVMKKVDYFVSVKQLNVQSNIKQDVQVLADPKFIQTAIFNIISNAIHYTIDHQHVYIKLYE
KPNGYALEVLNTGSQIDEDKLAHLFEPFYRANPDKHGLVQGSGLGLYIVKQILDKHQFPYGIQNTPQGVKCSIVFPKAM

Specific function: Member of the two-component regulatory system vanS/vanR. Activates the transcription of vanH, vanA and vanX in response to vancomycin which results in vancomycin resistance. VanS activates vanR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786600, Length=230, Percent_Identity=28.695652173913, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1786783, Length=265, Percent_Identity=24.1509433962264, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1788279, Length=234, Percent_Identity=26.9230769230769, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI145693157, Length=218, Percent_Identity=27.9816513761468, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI87082128, Length=238, Percent_Identity=25.2100840336134, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1788393, Length=265, Percent_Identity=24.5283018867925, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI87081816, Length=288, Percent_Identity=23.9583333333333, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1788713, Length=256, Percent_Identity=22.265625, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1786912, Length=246, Percent_Identity=20.7317073170732, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI48994928, Length=224, Percent_Identity=22.7678571428571, Blast_Score=62, Evalue=6e-11,
Organism=Escherichia coli, GI1789149, Length=250, Percent_Identity=24.4, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 54015; Mature: 53884

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATKSNRSKDNITKNIFIKTLLFCILLSLCLYQIIYFLASDYDKERFAKENGPSTTEQAD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCHHCC
SDKQNAQSKTNQNKAVSQGDMFNFQSSIIDYNALSTAINHLLQPSQTAKAKEHTQNISGK
CHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCC
NSASAPSTETEKQVATNDALHKQNAASKTNDAQDDPKLTDALQQLAPHIGATMLALSLLG
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SLIYAKIIAKPFQYMSDALKDIMNLDFSDKKLLNKNTDQTDFNLQVAASQVPNIVKNLHA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHCC
SNQDLRNELKKEQQLEQSRKEFMSMVSHELKTPIAAVMGQLDGMIHGIGAYKDRDKYLKR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCHHHHHHHH
SYEMMQDINILTEKMSELSKIQNPQFKPDLKVISLTNIIEDVMKKVDYFVSVKQLNVQSN
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IKQDVQVLADPKFIQTAIFNIISNAIHYTIDHQHVYIKLYEKPNGYALEVLNTGSQIDED
HHHHHHHHCCCHHHHHHHHHHHHHHHHEEECCCEEEEEEEECCCCCEEEEECCCCCCCHH
KLAHLFEPFYRANPDKHGLVQGSGLGLYIVKQILDKHQFPYGIQNTPQGVKCSIVFPKAM
HHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCC
>Mature Secondary Structure 
ATKSNRSKDNITKNIFIKTLLFCILLSLCLYQIIYFLASDYDKERFAKENGPSTTEQAD
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCHHCC
SDKQNAQSKTNQNKAVSQGDMFNFQSSIIDYNALSTAINHLLQPSQTAKAKEHTQNISGK
CHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCC
NSASAPSTETEKQVATNDALHKQNAASKTNDAQDDPKLTDALQQLAPHIGATMLALSLLG
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SLIYAKIIAKPFQYMSDALKDIMNLDFSDKKLLNKNTDQTDFNLQVAASQVPNIVKNLHA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHCC
SNQDLRNELKKEQQLEQSRKEFMSMVSHELKTPIAAVMGQLDGMIHGIGAYKDRDKYLKR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCHHHHHHHH
SYEMMQDINILTEKMSELSKIQNPQFKPDLKVISLTNIIEDVMKKVDYFVSVKQLNVQSN
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IKQDVQVLADPKFIQTAIFNIISNAIHYTIDHQHVYIKLYEKPNGYALEVLNTGSQIDED
HHHHHHHHCCCHHHHHHHHHHHHHHHHEEECCCEEEEEEEECCCCCEEEEECCCCCCCHH
KLAHLFEPFYRANPDKHGLVQGSGLGLYIVKQILDKHQFPYGIQNTPQGVKCSIVFPKAM
HHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8631706; 12663927 [H]