Click here to switch to the map view.

The map label for this gene is lipA

Identifier: 49187830

GI number: 49187830

Start: 4725283

End: 4726179

Strand: Reverse

Name: lipA

Synonym: BAS4840

Alternate gene names: 49187830

Gene position: 4726179-4725283 (Counterclockwise)

Preceding gene: 49187832

Following gene: 49187828

Centisome position: 90.39

GC content: 38.91

Gene sequence:

>897_bases
ATGACAAAACAAACAGAATATAAGCGCAAGCCCGAATGGTTGAAAATTAAGTTAAACACGAATGAAAACTATACAGGCTT
AAAGAAAATGATGCGTTCTAAGAATCTTCATACAGTTTGTGAAGAAGCGAAATGTCCGAATATTCATGAATGCTGGGCTG
TAAGAAAAACAGCAACATTTATGATTTTAGGAGCAGTATGTACACGTGCTTGTCGTTTCTGTGCGGTTAAAACAGGCTTA
CCAACAGAGCTTGATTTACAAGAGCCAGAACGCGTAGCAGATTCTGTAGTACAAATGGGCTTAAAGCACGTTGTTATAAC
AGCGGTTGCACGTGATGATTTAAAAGACGGGGGAGCAGCTGTTTTTGCTGAAACAGTACGCGCTGTACGTCGTAAAAATC
CATTTACGTCAATCGAAGTATTACCATCTGATATGGGTGGAGTAGAAGAAAACTTAAAAATGTTAATGGATGCAAAACCA
GATATTTTAAACCATAACATTGAAACAGTACGTCGATTATCTAACCGAGTTCGCGCTAGAGCAAAATATGACCGTTCATT
AGAGTTTTTACGTCGAGCGAAAGAAATGCAGCCTGATATTCCAACTAAATCGAGCATTATGGTCGGCTTAGGGGAAACGA
GAGAAGATTTAATTGAAGCAATGGATGACTTACGTGCAAACAATGTGGATATTTTAACTCTTGGACAATACCTACAACCA
TCTAAGAAGCATTTACCAGTTCTTAAATATTATCCACCAGCAGAATTTGCAGAGCTTAAAGAAATTGCACTTAGCAAAGG
ATTTAGCCACTGTGAAGCTGGCCCACTTGTGCGTTCTTCTTACCATGCGGACGAGCAAGTTCGTTCTGCAAAAGAAAAAA
CAGCAGAAGCAAAATAA

Upstream 100 bases:

>100_bases
TCTTCATTTGAGCAAATCGCTTTCATACGTTAGAATAGATATGTTACATTGAAAGAAGCGAACACGGTAAACTATTTCAT
ATTGTGCGGAGTTGATAACC

Downstream 100 bases:

>100_bases
TGAAAAAAGGGAGCTAACGATTAGCTCTCTTTTTGTTTATACTCCAAAACAGCATTTGAACATGTTAATAAAATCTTTTT
TAATTCCTCTGTGGATAATT

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MTKQTEYKRKPEWLKIKLNTNENYTGLKKMMRSKNLHTVCEEAKCPNIHECWAVRKTATFMILGAVCTRACRFCAVKTGL
PTELDLQEPERVADSVVQMGLKHVVITAVARDDLKDGGAAVFAETVRAVRRKNPFTSIEVLPSDMGGVEENLKMLMDAKP
DILNHNIETVRRLSNRVRARAKYDRSLEFLRRAKEMQPDIPTKSSIMVGLGETREDLIEAMDDLRANNVDILTLGQYLQP
SKKHLPVLKYYPPAEFAELKEIALSKGFSHCEAGPLVRSSYHADEQVRSAKEKTAEAK

Sequences:

>Translated_298_residues
MTKQTEYKRKPEWLKIKLNTNENYTGLKKMMRSKNLHTVCEEAKCPNIHECWAVRKTATFMILGAVCTRACRFCAVKTGL
PTELDLQEPERVADSVVQMGLKHVVITAVARDDLKDGGAAVFAETVRAVRRKNPFTSIEVLPSDMGGVEENLKMLMDAKP
DILNHNIETVRRLSNRVRARAKYDRSLEFLRRAKEMQPDIPTKSSIMVGLGETREDLIEAMDDLRANNVDILTLGQYLQP
SKKHLPVLKYYPPAEFAELKEIALSKGFSHCEAGPLVRSSYHADEQVRSAKEKTAEAK
>Mature_297_residues
TKQTEYKRKPEWLKIKLNTNENYTGLKKMMRSKNLHTVCEEAKCPNIHECWAVRKTATFMILGAVCTRACRFCAVKTGLP
TELDLQEPERVADSVVQMGLKHVVITAVARDDLKDGGAAVFAETVRAVRRKNPFTSIEVLPSDMGGVEENLKMLMDAKPD
ILNHNIETVRRLSNRVRARAKYDRSLEFLRRAKEMQPDIPTKSSIMVGLGETREDLIEAMDDLRANNVDILTLGQYLQPS
KKHLPVLKYYPPAEFAELKEIALSKGFSHCEAGPLVRSSYHADEQVRSAKEKTAEAK

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=289, Percent_Identity=44.9826989619377, Blast_Score=256, Evalue=2e-68,
Organism=Homo sapiens, GI37577164, Length=251, Percent_Identity=45.8167330677291, Blast_Score=228, Evalue=6e-60,
Organism=Escherichia coli, GI1786846, Length=294, Percent_Identity=51.7006802721088, Blast_Score=303, Evalue=8e-84,
Organism=Caenorhabditis elegans, GI32564533, Length=286, Percent_Identity=45.4545454545455, Blast_Score=238, Evalue=3e-63,
Organism=Saccharomyces cerevisiae, GI6324770, Length=286, Percent_Identity=46.8531468531469, Blast_Score=261, Evalue=8e-71,
Organism=Drosophila melanogaster, GI221513272, Length=283, Percent_Identity=46.2897526501767, Blast_Score=254, Evalue=4e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_BACAA (C3PDK2)

Other databases:

- EMBL:   CP001598
- RefSeq:   YP_002869212.1
- ProteinModelPortal:   C3PDK2
- EnsemblBacteria:   EBBACT00000126029
- GeneID:   7850538
- GenomeReviews:   CP001598_GR
- KEGG:   bai:BAA_5241
- GeneTree:   EBGT00050000002594
- ProtClustDB:   PRK05481
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 33701; Mature: 33570

Theoretical pI: Translated: 9.19; Mature: 9.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKQTEYKRKPEWLKIKLNTNENYTGLKKMMRSKNLHTVCEEAKCPNIHECWAVRKTATF
CCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHH
MILGAVCTRACRFCAVKTGLPTELDLQEPERVADSVVQMGLKHVVITAVARDDLKDGGAA
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
VFAETVRAVRRKNPFTSIEVLPSDMGGVEENLKMLMDAKPDILNHNIETVRRLSNRVRAR
HHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHH
AKYDRSLEFLRRAKEMQPDIPTKSSIMVGLGETREDLIEAMDDLRANNVDILTLGQYLQP
HHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCEEEEECCHHHCC
SKKHLPVLKYYPPAEFAELKEIALSKGFSHCEAGPLVRSSYHADEQVRSAKEKTAEAK
HHHCCCEEECCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TKQTEYKRKPEWLKIKLNTNENYTGLKKMMRSKNLHTVCEEAKCPNIHECWAVRKTATF
CCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHH
MILGAVCTRACRFCAVKTGLPTELDLQEPERVADSVVQMGLKHVVITAVARDDLKDGGAA
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
VFAETVRAVRRKNPFTSIEVLPSDMGGVEENLKMLMDAKPDILNHNIETVRRLSNRVRAR
HHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHH
AKYDRSLEFLRRAKEMQPDIPTKSSIMVGLGETREDLIEAMDDLRANNVDILTLGQYLQP
HHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCEEEEECCHHHCC
SKKHLPVLKYYPPAEFAELKEIALSKGFSHCEAGPLVRSSYHADEQVRSAKEKTAEAK
HHHCCCEEECCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA