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The map label for this gene is dnaB [H]

Identifier: 49187470

GI number: 49187470

Start: 4386599

End: 4388005

Strand: Reverse

Name: dnaB [H]

Synonym: BAS4475

Alternate gene names: 49187470

Gene position: 4388005-4386599 (Counterclockwise)

Preceding gene: 49187471

Following gene: 49187469

Centisome position: 83.92

GC content: 38.88

Gene sequence:

>1407_bases
ATGGAAAAACAGTCATGGATGGAGCTATTGCCGATTGATCGTTATAAAGTAAGTGCGAAAGGGTTATTGCATAATTACGA
CCGAAAAGTATTAACGATGTTATATCAGCCGTTAATAGGTAGTAGAGCTTTTAGCTTGTACATGACGCTATGGGGAGAGT
TAGAGCAAGATCGTGTATTTGGAAAAGAGAATACGCATCATTCCCTTATGGTGACTATGCAAATGCAGCTTCCCGAAGTA
TATGAGGAACGAGTAAAGTTAGAGGCAATTGGACTTTTGAAAGTATATATTAAGAAAGAAAAAGATATTCGAATGTTTAT
ATATGAGCTGCAACCACCTTTATCTCCGAAGCAGTTTTTTGATGATATTGTTTTAAGTATCTTTTTATACAATCGATTGA
GTCGGACAAAATATAATCAAGTAAAGCATTATTTCTTAGAAGAAGAATTTGATTTTGCATCTTATGAAAATGTTACGCGT
TCTTTTAACGATGTGTTTGGTTCGTTTAATCCAGGGCAGCTTGAGCATGCGCAAGAAGACCTTCGTATTCCAAAAACGAC
AGCTATGCCAAGTAATGAAAAGGGGGACGCTCCAAAAGTTTGGAATGATTTCTTTGATTTCTCCTTATTTGTAGACGGCT
TGTCCGCTCTTGTCCCTAAAAAGGCGATTACAGATCAAGTGCGAGAATGTGTCATTACACTTGCTTACGTGTATGATGTA
GATGTGTTATCGATGCAAAATATCGTTCTTGGTGCGGTGACAGAGATGCAGACAATTGATATGGAAAGGCTTAGAAAAGG
AGCACGTGATTGGTATCAGTTCGAAAACGGTCAAGCGTTACCTGTATTAAGTGAAAGAGTACAGCCTCACGCTGCACGTA
TGATGAAAGAAAAAGAGCCTTCTACGCAAGAAGAGATGTTAATCAAACAGCTAGAGGAAATCTCACCAAAGCAACTATTG
AAAGAAATCTCTGGTGGTGCAGAGGCGACGAAGGCTGACTTGCAAATCGTTGAAGATGTAATGATCAATCAAAAATTAAC
GCCAGGTGTTGTGAATGTACTTATTTATTACGTTATGCTACGCTCAGATATGAAACTTGCGAAAACGTATGTGGAGAAGA
TTGCTGGACATTGGGCACGTAAAAAGGTCGGTACAGTAGCTGAAGCAATGGCGTTAGCGAAAGAAGAAAACCGTCAATAT
CAAGAATGGGCTGAGACGAAGAAGAAAGGCCGTACATCGAAGAAAACGGTACGAAAAGAAATGGTACCAGATTGGCTGAA
AGAAGAGCCGAAAGAACAAGAGAAAGAAACTGTGAAAAAAGATGCAAGTGCTGAAAAAGGTGCAAGTACGTTAGAGGATG
AACGGAAACGACTAGAAGAAGTGTTAAAAAAATATAAGCGTGATTAA

Upstream 100 bases:

>100_bases
CGTAAGTAATGAGATAAGAGAGCTAGAAGCGAAAGCTAATAGCTCTTTTTCGCTAAGAAATTATATAGTGAAGATTGGAT
GAGAGAAAGGAAAAGCAAGA

Downstream 100 bases:

>100_bases
TAGAAGAGAATGAACGCTCTTTGAGGTGAGGAAATGGAGCATATTCAAAATTCATTTGCAAAATTAATGGAAAATGAAAA
TTTTAAAAATAGATATGAAG

Product: DNA replication protein DnaB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MEKQSWMELLPIDRYKVSAKGLLHNYDRKVLTMLYQPLIGSRAFSLYMTLWGELEQDRVFGKENTHHSLMVTMQMQLPEV
YEERVKLEAIGLLKVYIKKEKDIRMFIYELQPPLSPKQFFDDIVLSIFLYNRLSRTKYNQVKHYFLEEEFDFASYENVTR
SFNDVFGSFNPGQLEHAQEDLRIPKTTAMPSNEKGDAPKVWNDFFDFSLFVDGLSALVPKKAITDQVRECVITLAYVYDV
DVLSMQNIVLGAVTEMQTIDMERLRKGARDWYQFENGQALPVLSERVQPHAARMMKEKEPSTQEEMLIKQLEEISPKQLL
KEISGGAEATKADLQIVEDVMINQKLTPGVVNVLIYYVMLRSDMKLAKTYVEKIAGHWARKKVGTVAEAMALAKEENRQY
QEWAETKKKGRTSKKTVRKEMVPDWLKEEPKEQEKETVKKDASAEKGASTLEDERKRLEEVLKKYKRD

Sequences:

>Translated_468_residues
MEKQSWMELLPIDRYKVSAKGLLHNYDRKVLTMLYQPLIGSRAFSLYMTLWGELEQDRVFGKENTHHSLMVTMQMQLPEV
YEERVKLEAIGLLKVYIKKEKDIRMFIYELQPPLSPKQFFDDIVLSIFLYNRLSRTKYNQVKHYFLEEEFDFASYENVTR
SFNDVFGSFNPGQLEHAQEDLRIPKTTAMPSNEKGDAPKVWNDFFDFSLFVDGLSALVPKKAITDQVRECVITLAYVYDV
DVLSMQNIVLGAVTEMQTIDMERLRKGARDWYQFENGQALPVLSERVQPHAARMMKEKEPSTQEEMLIKQLEEISPKQLL
KEISGGAEATKADLQIVEDVMINQKLTPGVVNVLIYYVMLRSDMKLAKTYVEKIAGHWARKKVGTVAEAMALAKEENRQY
QEWAETKKKGRTSKKTVRKEMVPDWLKEEPKEQEKETVKKDASAEKGASTLEDERKRLEEVLKKYKRD
>Mature_468_residues
MEKQSWMELLPIDRYKVSAKGLLHNYDRKVLTMLYQPLIGSRAFSLYMTLWGELEQDRVFGKENTHHSLMVTMQMQLPEV
YEERVKLEAIGLLKVYIKKEKDIRMFIYELQPPLSPKQFFDDIVLSIFLYNRLSRTKYNQVKHYFLEEEFDFASYENVTR
SFNDVFGSFNPGQLEHAQEDLRIPKTTAMPSNEKGDAPKVWNDFFDFSLFVDGLSALVPKKAITDQVRECVITLAYVYDV
DVLSMQNIVLGAVTEMQTIDMERLRKGARDWYQFENGQALPVLSERVQPHAARMMKEKEPSTQEEMLIKQLEEISPKQLL
KEISGGAEATKADLQIVEDVMINQKLTPGVVNVLIYYVMLRSDMKLAKTYVEKIAGHWARKKVGTVAEAMALAKEENRQY
QEWAETKKKGRTSKKTVRKEMVPDWLKEEPKEQEKETVKKDASAEKGASTLEDERKRLEEVLKKYKRD

Specific function: Probable component of primosome involved in the initiation of DNA replication. It is essential for both replication initiation and membrane attachment of the origin region of the chromosome and plasmid pUB110 [H]

COG id: COG3611

COG function: function code L; Replication initiation/membrane attachment protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006343 [H]

Pfam domain/function: PF07261 DnaB_2 [H]

EC number: NA

Molecular weight: Translated: 54547; Mature: 54547

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HCHHHHHCCCHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure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HCHHHHHCCCHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3027697; 3027671; 8969504; 9387221; 9384377 [H]