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The map label for this gene is yrrN [H]

Identifier: 49187274

GI number: 49187274

Start: 4188897

End: 4189826

Strand: Reverse

Name: yrrN [H]

Synonym: BAS4278

Alternate gene names: 49187274

Gene position: 4189826-4188897 (Counterclockwise)

Preceding gene: 49187275

Following gene: 49187273

Centisome position: 80.13

GC content: 36.77

Gene sequence:

>930_bases
ATGAAGAAACCTGAATTGTTAGTAACGCCAAAAGCAGTGGCGGATATCGAACCTCTTGCGAAAGCAGGAGTAGATGCGGT
AATGATCGGCGAGCAAAAGTTTGGTTTACGTTTAGCAGGTGAGTTTTCACGTGAAGATGTGAAACAAGCTGTGAAAATTG
CACATGAAAATGGTGTGAAAGTATACGTAGCAATGAACGCTATGTTCCACAACGATAAAGTAGAAGAATTAACAGACTAT
GTTGCATTTTTAAACGAAGTACATGTAGATGCAATTGTTTTTGGAGACCCAGCAGTATTAATGGCGGTCCGCGAAGTAGC
GCCAAATATGCAATTGCACTGGAATGCAGAAACAACAGCAACAAATTGGTTTACTTGTAACTACTGGGGAAAAAGAGGAG
CAAAACGTGCTGTATTAGCTCGTGAGCTTAGCTTAGATGAAATTGTTGAGTTAAAAGAAAATGCTGAAGTGGAACTTGAA
GTACAAATTCACGGTATGACTTGTATGTTCCAATCGAAGCGTTCATTAGTAGGAAACTACTTTGAGTATCAAGGACGTCA
TCTTGACATTGAAAAGAAAAAATATGAAGAGAATATGTTCTTATATGACCCAGAGCGTAACAATAAATATCCAATTTATG
AAGATGAAAATGGTACTCACATTATGAGTCCAAACGATATTTGTTTCATCGATGAGTTAGAAGAGCTAATTGATGCTGAA
ATCGACAGCTTAAAAATTGATGGTGTACTAAAAAGCTCTGAATACATTATTGAGGTAACGAAGAAATATCGTAAAGCAAT
TGATTTATGTGTAGAAGATCGTGATGCGTATTATGACGTGAAAGACGATTTATATAAAGAGATAGAAGAAATGCAACCGG
TAAATCGTCCGTTAGATACAGGATTCTTCTTTAAAGAGACGGTTTACTAA

Upstream 100 bases:

>100_bases
ACGTACAATGAATGGCTTATGAACCATGAAGGATATGATACAACGATTTTCCCAATTGGTGATGGAGTAGCTGTTAGTAA
AAAGAGAGGGTGACCGAGGT

Downstream 100 bases:

>100_bases
ACGAGGAGGGTGTAGGAAATGACTGTACAAGAAATTTCACGAGTAATCGATGGCAAACGCGTTATTGTGAAGAAACCTGA
ACTGTTAATCCCTGCGGGTA

Product: U32 family peptidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MKKPELLVTPKAVADIEPLAKAGVDAVMIGEQKFGLRLAGEFSREDVKQAVKIAHENGVKVYVAMNAMFHNDKVEELTDY
VAFLNEVHVDAIVFGDPAVLMAVREVAPNMQLHWNAETTATNWFTCNYWGKRGAKRAVLARELSLDEIVELKENAEVELE
VQIHGMTCMFQSKRSLVGNYFEYQGRHLDIEKKKYEENMFLYDPERNNKYPIYEDENGTHIMSPNDICFIDELEELIDAE
IDSLKIDGVLKSSEYIIEVTKKYRKAIDLCVEDRDAYYDVKDDLYKEIEEMQPVNRPLDTGFFFKETVY

Sequences:

>Translated_309_residues
MKKPELLVTPKAVADIEPLAKAGVDAVMIGEQKFGLRLAGEFSREDVKQAVKIAHENGVKVYVAMNAMFHNDKVEELTDY
VAFLNEVHVDAIVFGDPAVLMAVREVAPNMQLHWNAETTATNWFTCNYWGKRGAKRAVLARELSLDEIVELKENAEVELE
VQIHGMTCMFQSKRSLVGNYFEYQGRHLDIEKKKYEENMFLYDPERNNKYPIYEDENGTHIMSPNDICFIDELEELIDAE
IDSLKIDGVLKSSEYIIEVTKKYRKAIDLCVEDRDAYYDVKDDLYKEIEEMQPVNRPLDTGFFFKETVY
>Mature_309_residues
MKKPELLVTPKAVADIEPLAKAGVDAVMIGEQKFGLRLAGEFSREDVKQAVKIAHENGVKVYVAMNAMFHNDKVEELTDY
VAFLNEVHVDAIVFGDPAVLMAVREVAPNMQLHWNAETTATNWFTCNYWGKRGAKRAVLARELSLDEIVELKENAEVELE
VQIHGMTCMFQSKRSLVGNYFEYQGRHLDIEKKKYEENMFLYDPERNNKYPIYEDENGTHIMSPNDICFIDELEELIDAE
IDSLKIDGVLKSSEYIIEVTKKYRKAIDLCVEDRDAYYDVKDDLYKEIEEMQPVNRPLDTGFFFKETVY

Specific function: Unknown

COG id: COG0826

COG function: function code O; Collagenase and related proteases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U32 family [H]

Homologues:

Organism=Escherichia coli, GI1788397, Length=310, Percent_Identity=31.9354838709677, Blast_Score=131, Evalue=7e-32,
Organism=Escherichia coli, GI87081910, Length=253, Percent_Identity=28.0632411067194, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1789548, Length=297, Percent_Identity=25.5892255892256, Blast_Score=94, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001539 [H]

Pfam domain/function: PF01136 Peptidase_U32 [H]

EC number: 3.4.-.- [C]

Molecular weight: Translated: 35663; Mature: 35663

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS00018 EF_HAND_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKPELLVTPKAVADIEPLAKAGVDAVMIGEQKFGLRLAGEFSREDVKQAVKIAHENGVK
CCCCCEEECCHHHHHHHHHHHCCCCEEEECCCCCCEEEECCCCHHHHHHHHHHHHCCCCE
VYVAMNAMFHNDKVEELTDYVAFLNEVHVDAIVFGDPAVLMAVREVAPNMQLHWNAETTA
EEEEEEEEECCCHHHHHHHHHHHHHHHCEEEEEECCHHHHHHHHHHCCCCEEEECCCCCE
TNWFTCNYWGKRGAKRAVLARELSLDEIVELKENAEVELEVQIHGMTCMFQSKRSLVGNY
ECCEEEECCCCCCCHHHHHHHHCCHHHHHHHHCCCCEEEEEEEECEEEEEHHHHHHHHHH
FEYQGRHLDIEKKKYEENMFLYDPERNNKYPIYEDENGTHIMSPNDICFIDELEELIDAE
HHHCCCEECCHHHHCCCCEEEECCCCCCCCCEEECCCCCEEECCCCEEEHHHHHHHHCCC
IDSLKIDGVLKSSEYIIEVTKKYRKAIDLCVEDRDAYYDVKDDLYKEIEEMQPVNRPLDT
CCCEEECEEECCCHHHHHHHHHHHHHHHHHCCCCCCEECHHHHHHHHHHHCCCCCCCCCC
GFFFKETVY
CCEEEECCC
>Mature Secondary Structure
MKKPELLVTPKAVADIEPLAKAGVDAVMIGEQKFGLRLAGEFSREDVKQAVKIAHENGVK
CCCCCEEECCHHHHHHHHHHHCCCCEEEECCCCCCEEEECCCCHHHHHHHHHHHHCCCCE
VYVAMNAMFHNDKVEELTDYVAFLNEVHVDAIVFGDPAVLMAVREVAPNMQLHWNAETTA
EEEEEEEEECCCHHHHHHHHHHHHHHHCEEEEEECCHHHHHHHHHHCCCCEEEECCCCCE
TNWFTCNYWGKRGAKRAVLARELSLDEIVELKENAEVELEVQIHGMTCMFQSKRSLVGNY
ECCEEEECCCCCCCHHHHHHHHCCHHHHHHHHCCCCEEEEEEEECEEEEEHHHHHHHHHH
FEYQGRHLDIEKKKYEENMFLYDPERNNKYPIYEDENGTHIMSPNDICFIDELEELIDAE
HHHCCCEECCHHHHCCCCEEEECCCCCCCCCEEECCCCCEEECCCCEEEHHHHHHHHCCC
IDSLKIDGVLKSSEYIIEVTKKYRKAIDLCVEDRDAYYDVKDDLYKEIEEMQPVNRPLDT
CCCEEECEEECCCHHHHHHHHHHHHHHHHHCCCCCCEECHHHHHHHHHHHCCCCCCCCCC
GFFFKETVY
CCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]