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The map label for this gene is recN [H]

Identifier: 49187076

GI number: 49187076

Start: 4011949

End: 4013688

Strand: Reverse

Name: recN [H]

Synonym: BAS4078

Alternate gene names: 49187076

Gene position: 4013688-4011949 (Counterclockwise)

Preceding gene: 49187077

Following gene: 49187075

Centisome position: 76.76

GC content: 36.09

Gene sequence:

>1740_bases
TTGTTATCGGAATTATCGATTAGAAACTTTGCTATTATTGAGGCATTAAATATTTCTTTTCAAAAAGGATTAACGGTTTT
AAGTGGTGAAACAGGGGCCGGAAAATCGATTATTATTGATGCGATTAGTTTACTTGTAGGTGGCCGCGGTTCGGCAGAAT
TTGTTCGATACGGAACAGAAAAAGCTGAGATAGAGGGGCTATTTTATGTGGAAGATGATAAGCATCCATGTATTGAAAAG
GCAGAAGAATTGGATATAGAAATAGAAGACGGCATGATTATTTTGAAGCGTGATATCGCTGCAAACGGAAAAAGCGTATG
TCGTGTAAATGGAAAACTAGTTACCCTTAGTGTATTAAAAGAAATTGGAAAAACACTTGTTGATATTCATGGGCAACACG
AAACGCAAGATTTAATGAATGAAGAACGTCATCTATTTATGCTCGATCATTTTGATGGGGAGCGTATCGTAAAACAATTG
GATATATACCAAAACGTATACGCTGACTATGAGAAGTTAAAAAAACAATTGAAATCGTTAAGTGAAAATGAACAACAAAT
GGCGCATCGCTTAGATTTAATTCAATTCCAGCATGAAGAAATCCGTAAGGCGGATTTAAAGATGGATGAAGAAAATAACT
TAACTGAAGAACGATTGCAAATTTCTAATTTCGAAAAGATTTATAAAGCATTAGGTGACGCATATCGCTCGTTAAGTGCT
GATGGACAAGGGTTAGATAATGTAAGAAGTGCAATGGGACAAATGGAGAGTATTACACATTTAGACGAAGTGTACCAAGA
AAATCATGATTCAATTGCAAATAGCTATTACCTATTAGAAGAAGTTGCATATCAACTAAGAGAAAAACTCGATATGATGG
AATATGACCCAAATCGTTTAGACGAAATTGAAACGCGTTTAAATGAAATTCGTATGCTAAAGAGAAAATATGGAAATACT
GTAGAAGAGATATTAGCGTATGCTGATAAAATTGAACAAGAGATTTTTACGATTGAAAATAAAGACGTTCATATTGAAAC
GACGAAGAAACAGTTGAAGGAATTAGAAAGTGTTATTCTGAAAGAAGCAACGTTGTTAAGTAATATGCGTCATGAACTTG
CAGAGCATCTTACAAATGCCATTCATCAGGAATTAAAAGAATTGTATATGGAAAAAACAAAATTTGAAGTGAGAATCATA
AAGCGAGAAGGAAATGCGGAAGAGCCTCTCGTGGAGGGGGCACCGGTAAGGCTTACAGCGGACGGTTATGATCATGTGGA
ATTTTATATTTCAACCAATCCAGGTGAGCCGCTAAAACCACTTTCAAAGGTCGCTTCTGGCGGAGAGTTGTTCCGTATTA
TTTTAGCTTTAAAAAGTATTTTTTCTAAGCATCAAGGTGTTGCATCTGTTATTTTTGATGAAGTGGATACTGGTGTAAGT
GGCCGGGTCGCACAGGCTATTGCGGAAAAAATTTACCGAGTATCAGTAAACTCACAAGTACTTTGTATTACGCACTTACC
TCAAGTAGCTTCAATGGCGGATTCGCATTTATTTATCCGAAAACAAGTAGCGAATGATCGAACAATTACATCCGTTACCG
TTTTAACTATGGAGGATAAAGTAACAGAAATAGCTCGAATGATTTCTGGTGTGGAAATTACAGATTTAACGACAGAACAT
GCGAAAGAATTACTTACGCAAGCGCATCATTTTAAACAGACAGCAGAGGCTATCCAGTAA

Upstream 100 bases:

>100_bases
AAAGTAATTTATACATACAAACATATAGTATGACTATTTGAATAAAGAACTGAGAAAAGCCCTTAATAGGGGATTAGTTT
ATAACGAGGTGAATGGGGCA

Downstream 100 bases:

>100_bases
TGGATAGCTTTTTTTCTCAAATGATGATTTCTTAATTCTTCCGGTTATAAATAAAGCATTGCAAGGAGAGATTAAAAAGT
GAAGCCAAGAGGCTGAATGT

Product: DNA repair protein RecN

Products: NA

Alternate protein names: Recombination protein N [H]

Number of amino acids: Translated: 579; Mature: 579

Protein sequence:

>579_residues
MLSELSIRNFAIIEALNISFQKGLTVLSGETGAGKSIIIDAISLLVGGRGSAEFVRYGTEKAEIEGLFYVEDDKHPCIEK
AEELDIEIEDGMIILKRDIAANGKSVCRVNGKLVTLSVLKEIGKTLVDIHGQHETQDLMNEERHLFMLDHFDGERIVKQL
DIYQNVYADYEKLKKQLKSLSENEQQMAHRLDLIQFQHEEIRKADLKMDEENNLTEERLQISNFEKIYKALGDAYRSLSA
DGQGLDNVRSAMGQMESITHLDEVYQENHDSIANSYYLLEEVAYQLREKLDMMEYDPNRLDEIETRLNEIRMLKRKYGNT
VEEILAYADKIEQEIFTIENKDVHIETTKKQLKELESVILKEATLLSNMRHELAEHLTNAIHQELKELYMEKTKFEVRII
KREGNAEEPLVEGAPVRLTADGYDHVEFYISTNPGEPLKPLSKVASGGELFRIILALKSIFSKHQGVASVIFDEVDTGVS
GRVAQAIAEKIYRVSVNSQVLCITHLPQVASMADSHLFIRKQVANDRTITSVTVLTMEDKVTEIARMISGVEITDLTTEH
AKELLTQAHHFKQTAEAIQ

Sequences:

>Translated_579_residues
MLSELSIRNFAIIEALNISFQKGLTVLSGETGAGKSIIIDAISLLVGGRGSAEFVRYGTEKAEIEGLFYVEDDKHPCIEK
AEELDIEIEDGMIILKRDIAANGKSVCRVNGKLVTLSVLKEIGKTLVDIHGQHETQDLMNEERHLFMLDHFDGERIVKQL
DIYQNVYADYEKLKKQLKSLSENEQQMAHRLDLIQFQHEEIRKADLKMDEENNLTEERLQISNFEKIYKALGDAYRSLSA
DGQGLDNVRSAMGQMESITHLDEVYQENHDSIANSYYLLEEVAYQLREKLDMMEYDPNRLDEIETRLNEIRMLKRKYGNT
VEEILAYADKIEQEIFTIENKDVHIETTKKQLKELESVILKEATLLSNMRHELAEHLTNAIHQELKELYMEKTKFEVRII
KREGNAEEPLVEGAPVRLTADGYDHVEFYISTNPGEPLKPLSKVASGGELFRIILALKSIFSKHQGVASVIFDEVDTGVS
GRVAQAIAEKIYRVSVNSQVLCITHLPQVASMADSHLFIRKQVANDRTITSVTVLTMEDKVTEIARMISGVEITDLTTEH
AKELLTQAHHFKQTAEAIQ
>Mature_579_residues
MLSELSIRNFAIIEALNISFQKGLTVLSGETGAGKSIIIDAISLLVGGRGSAEFVRYGTEKAEIEGLFYVEDDKHPCIEK
AEELDIEIEDGMIILKRDIAANGKSVCRVNGKLVTLSVLKEIGKTLVDIHGQHETQDLMNEERHLFMLDHFDGERIVKQL
DIYQNVYADYEKLKKQLKSLSENEQQMAHRLDLIQFQHEEIRKADLKMDEENNLTEERLQISNFEKIYKALGDAYRSLSA
DGQGLDNVRSAMGQMESITHLDEVYQENHDSIANSYYLLEEVAYQLREKLDMMEYDPNRLDEIETRLNEIRMLKRKYGNT
VEEILAYADKIEQEIFTIENKDVHIETTKKQLKELESVILKEATLLSNMRHELAEHLTNAIHQELKELYMEKTKFEVRII
KREGNAEEPLVEGAPVRLTADGYDHVEFYISTNPGEPLKPLSKVASGGELFRIILALKSIFSKHQGVASVIFDEVDTGVS
GRVAQAIAEKIYRVSVNSQVLCITHLPQVASMADSHLFIRKQVANDRTITSVTVLTMEDKVTEIARMISGVEITDLTTEH
AKELLTQAHHFKQTAEAIQ

Specific function: Involved in recombinational repair of damaged DNA. Seems to be the first protein recruited to repair centers, foci that are the site of double-strand DNA break(s), followed by recO and then recF [H]

COG id: COG0497

COG function: function code L; ATPase involved in DNA repair

Gene ontology:

Cell location: Cytoplasm, nucleoid. Note=Cytoplasmically located in untreated cells. Recruited to foci following treatment with DNA damaging agents; these foci are presumably the breaks themselves. They are almost always located within nucleoids [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recN family [H]

Homologues:

Organism=Escherichia coli, GI48994901, Length=573, Percent_Identity=32.6352530541012, Blast_Score=293, Evalue=2e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004604
- InterPro:   IPR003395 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 65836; Mature: 65836

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSELSIRNFAIIEALNISFQKGLTVLSGETGAGKSIIIDAISLLVGGRGSAEFVRYGTE
CCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHCCCC
KAEIEGLFYVEDDKHPCIEKAEELDIEIEDGMIILKRDIAANGKSVCRVNGKLVTLSVLK
HHHCCEEEEEECCCCCHHHHHHHCCEEEECCEEEEEECCCCCCCCEEEECCEEEHHHHHH
EIGKTLVDIHGQHETQDLMNEERHLFMLDHFDGERIVKQLDIYQNVYADYEKLKKQLKSL
HHHHHHHHHCCCCHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SENEQQMAHRLDLIQFQHEEIRKADLKMDEENNLTEERLQISNFEKIYKALGDAYRSLSA
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
DGQGLDNVRSAMGQMESITHLDEVYQENHDSIANSYYLLEEVAYQLREKLDMMEYDPNRL
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
DEIETRLNEIRMLKRKYGNTVEEILAYADKIEQEIFTIENKDVHIETTKKQLKELESVIL
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEECCCCEEEEHHHHHHHHHHHHHH
KEATLLSNMRHELAEHLTNAIHQELKELYMEKTKFEVRIIKREGNAEEPLVEGAPVRLTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCCEEEEE
DGYDHVEFYISTNPGEPLKPLSKVASGGELFRIILALKSIFSKHQGVASVIFDEVDTGVS
CCCCEEEEEEECCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCC
GRVAQAIAEKIYRVSVNSQVLCITHLPQVASMADSHLFIRKQVANDRTITSVTVLTMEDK
HHHHHHHHHHHHHEECCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEHHH
VTEIARMISGVEITDLTTEHAKELLTQAHHFKQTAEAIQ
HHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLSELSIRNFAIIEALNISFQKGLTVLSGETGAGKSIIIDAISLLVGGRGSAEFVRYGTE
CCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHCCCC
KAEIEGLFYVEDDKHPCIEKAEELDIEIEDGMIILKRDIAANGKSVCRVNGKLVTLSVLK
HHHCCEEEEEECCCCCHHHHHHHCCEEEECCEEEEEECCCCCCCCEEEECCEEEHHHHHH
EIGKTLVDIHGQHETQDLMNEERHLFMLDHFDGERIVKQLDIYQNVYADYEKLKKQLKSL
HHHHHHHHHCCCCHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SENEQQMAHRLDLIQFQHEEIRKADLKMDEENNLTEERLQISNFEKIYKALGDAYRSLSA
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
DGQGLDNVRSAMGQMESITHLDEVYQENHDSIANSYYLLEEVAYQLREKLDMMEYDPNRL
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
DEIETRLNEIRMLKRKYGNTVEEILAYADKIEQEIFTIENKDVHIETTKKQLKELESVIL
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEECCCCEEEEHHHHHHHHHHHHHH
KEATLLSNMRHELAEHLTNAIHQELKELYMEKTKFEVRIIKREGNAEEPLVEGAPVRLTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCCEEEEE
DGYDHVEFYISTNPGEPLKPLSKVASGGELFRIILALKSIFSKHQGVASVIFDEVDTGVS
CCCCEEEEEEECCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCC
GRVAQAIAEKIYRVSVNSQVLCITHLPQVASMADSHLFIRKQVANDRTITSVTVLTMEDK
HHHHHHHHHHHHHEECCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEHHH
VTEIARMISGVEITDLTTEHAKELLTQAHHFKQTAEAIQ
HHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2106508; 8969508; 9384377; 2507400; 9044256 [H]