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The map label for this gene is bfmBAA [H]

Identifier: 49187065

GI number: 49187065

Start: 3998166

End: 3999167

Strand: Reverse

Name: bfmBAA [H]

Synonym: BAS4067

Alternate gene names: 49187065

Gene position: 3999167-3998166 (Counterclockwise)

Preceding gene: 49187066

Following gene: 49187064

Centisome position: 76.49

GC content: 40.02

Gene sequence:

>1002_bases
ATGGCAGAAGTAAAAGAAAAGCGCCATGAAGAGCTTGGCTTAAGTGATGAGCAAGTGTTAGAAATGTTCCGTACGATGTT
ACTTGCACGTAAAATCGACGAACGTATGTGGTTATTAAACCGTGCTGGTAAAATTCCATTCGTAATTTCTTGTCAAGGAC
AAGAGGCAGCACAAGTTGGAGCAGCATTTGCTCTTGATAGAGAGAAAGATTATGCATTACCATACTACCGTGATATGGGT
GTTGTACTAGCGTTTGGTATGACAGCGAAAGAACTGATGCTATCTGGTTTCGCGAAAGCTGGAGATCCAAACTCTGGTGG
TCGTCAAATGCCTGGTCACTTCGGTCAAAAGAAAAATCGTATTGTGACAGGTTCATCTCCAGTAACAACGCAAGTACCAC
ATGCAGTTGGTATTGCATTAGCTGGAAAAATGGAAAAGAAAGATTTAGTAACGTTTGTTACATTCGGTGAAGGTTCTTCT
AACCAAGGTGATTTCCATGAAGGTGCAAACTTTGCTGGCGTACACAAGTTACCTGTTATTTTCATGTGTGAAAATAATAA
ATACGCAATCTCTATTCCAGTTGAAAAACAATTAGCATGTAAAAATGTATCAGACCGTGCAATTGGATACGGTATGCCTG
GATATACAGTAGACGGAAACGATCCGCTTGCGGTATATAAAGCTGTAAAAGAAGCAGCAGACCGTGGCCGCCGTGGTGAA
GGACCAACTTTAATTGAAACAGTATCATATCGTTTAACAGCACATTCAAGTGACGATGATGATCGTGTTTATCGTGATAA
AGAAGAAGTAGAAGAAGCGAAGAAAAATGATTCAATTGTAACATTTGCTGCTTATTTAAAAGAGGTTGGCGTGTTAACTG
AGGAGTCTGAAAAACAAATGTTAGACGAAATTATGCATATCGTAAACGAAGCAACAGAATATGCAGAAAATGCTCCGTAT
GCAGCACCTGAAGATGCATTGAAGCACGTATACGCAGAATAG

Upstream 100 bases:

>100_bases
TCCGCATCCATCATTATCTGAGGCGATTGGTGAAGCAGCATTAGCTGTAGATGGAAAAGCGTTACACGCATAAAAATGTG
GATTTAGGAGGTTATGAAAA

Downstream 100 bases:

>100_bases
GGGGGAACGTTTCATGGCTGTAATGTCTTATATTGATGCTATTACATTAGCAATGCGCGAGGAAATGGAACGCGATGAGA
AAGTATTCGTTTTAGGTGAA

Product: 3-methyl-2-oxobutanoate dehydrogenase subunit alpha

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain; BCKDH E1-alpha [H]

Number of amino acids: Translated: 333; Mature: 332

Protein sequence:

>333_residues
MAEVKEKRHEELGLSDEQVLEMFRTMLLARKIDERMWLLNRAGKIPFVISCQGQEAAQVGAAFALDREKDYALPYYRDMG
VVLAFGMTAKELMLSGFAKAGDPNSGGRQMPGHFGQKKNRIVTGSSPVTTQVPHAVGIALAGKMEKKDLVTFVTFGEGSS
NQGDFHEGANFAGVHKLPVIFMCENNKYAISIPVEKQLACKNVSDRAIGYGMPGYTVDGNDPLAVYKAVKEAADRGRRGE
GPTLIETVSYRLTAHSSDDDDRVYRDKEEVEEAKKNDSIVTFAAYLKEVGVLTEESEKQMLDEIMHIVNEATEYAENAPY
AAPEDALKHVYAE

Sequences:

>Translated_333_residues
MAEVKEKRHEELGLSDEQVLEMFRTMLLARKIDERMWLLNRAGKIPFVISCQGQEAAQVGAAFALDREKDYALPYYRDMG
VVLAFGMTAKELMLSGFAKAGDPNSGGRQMPGHFGQKKNRIVTGSSPVTTQVPHAVGIALAGKMEKKDLVTFVTFGEGSS
NQGDFHEGANFAGVHKLPVIFMCENNKYAISIPVEKQLACKNVSDRAIGYGMPGYTVDGNDPLAVYKAVKEAADRGRRGE
GPTLIETVSYRLTAHSSDDDDRVYRDKEEVEEAKKNDSIVTFAAYLKEVGVLTEESEKQMLDEIMHIVNEATEYAENAPY
AAPEDALKHVYAE
>Mature_332_residues
AEVKEKRHEELGLSDEQVLEMFRTMLLARKIDERMWLLNRAGKIPFVISCQGQEAAQVGAAFALDREKDYALPYYRDMGV
VLAFGMTAKELMLSGFAKAGDPNSGGRQMPGHFGQKKNRIVTGSSPVTTQVPHAVGIALAGKMEKKDLVTFVTFGEGSSN
QGDFHEGANFAGVHKLPVIFMCENNKYAISIPVEKQLACKNVSDRAIGYGMPGYTVDGNDPLAVYKAVKEAADRGRRGEG
PTLIETVSYRLTAHSSDDDDRVYRDKEEVEEAKKNDSIVTFAAYLKEVGVLTEESEKQMLDEIMHIVNEATEYAENAPYA
APEDALKHVYAE

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCKDHA family [H]

Homologues:

Organism=Homo sapiens, GI11386135, Length=325, Percent_Identity=38.4615384615385, Blast_Score=205, Evalue=4e-53,
Organism=Homo sapiens, GI258645172, Length=325, Percent_Identity=38.4615384615385, Blast_Score=201, Evalue=5e-52,
Organism=Homo sapiens, GI4885543, Length=321, Percent_Identity=30.5295950155763, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI291084742, Length=321, Percent_Identity=31.7757009345794, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI4505685, Length=321, Percent_Identity=31.7757009345794, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI291084744, Length=328, Percent_Identity=31.0975609756098, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI291084757, Length=172, Percent_Identity=34.3023255813954, Blast_Score=100, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI86563355, Length=321, Percent_Identity=39.5638629283489, Blast_Score=205, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI86563357, Length=321, Percent_Identity=39.5638629283489, Blast_Score=205, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI17536047, Length=322, Percent_Identity=32.6086956521739, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI32564172, Length=322, Percent_Identity=32.6086956521739, Blast_Score=136, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6321026, Length=325, Percent_Identity=32.9230769230769, Blast_Score=144, Evalue=2e-35,
Organism=Drosophila melanogaster, GI21355903, Length=323, Percent_Identity=33.1269349845201, Blast_Score=170, Evalue=1e-42,
Organism=Drosophila melanogaster, GI24639744, Length=314, Percent_Identity=31.2101910828025, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI28571106, Length=314, Percent_Identity=31.2101910828025, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24639740, Length=314, Percent_Identity=31.2101910828025, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24639748, Length=292, Percent_Identity=32.8767123287671, Blast_Score=125, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24639746, Length=272, Percent_Identity=32.7205882352941, Blast_Score=124, Evalue=8e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001017 [H]

Pfam domain/function: PF00676 E1_dh [H]

EC number: =1.2.4.4 [H]

Molecular weight: Translated: 36754; Mature: 36623

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEVKEKRHEELGLSDEQVLEMFRTMLLARKIDERMWLLNRAGKIPFVISCQGQEAAQVG
CCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHH
AAFALDREKDYALPYYRDMGVVLAFGMTAKELMLSGFAKAGDPNSGGRQMPGHFGQKKNR
HHHEECCCCCCCCCHHHHCHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCE
IVTGSSPVTTQVPHAVGIALAGKMEKKDLVTFVTFGEGSSNQGDFHEGANFAGVHKLPVI
EEECCCCCCCCCCHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCHHCCCCCCCEECCCEE
FMCENNKYAISIPVEKQLACKNVSDRAIGYGMPGYTVDGNDPLAVYKAVKEAADRGRRGE
EEECCCCEEEECCCHHHHHHCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHCCCCCC
GPTLIETVSYRLTAHSSDDDDRVYRDKEEVEEAKKNDSIVTFAAYLKEVGVLTEESEKQM
CCHHHHHHEEEEEECCCCCCHHHHCCHHHHHHHHCCCCEEHHHHHHHHHCCCCCHHHHHH
LDEIMHIVNEATEYAENAPYAAPEDALKHVYAE
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
AEVKEKRHEELGLSDEQVLEMFRTMLLARKIDERMWLLNRAGKIPFVISCQGQEAAQVG
CCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHH
AAFALDREKDYALPYYRDMGVVLAFGMTAKELMLSGFAKAGDPNSGGRQMPGHFGQKKNR
HHHEECCCCCCCCCHHHHCHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCE
IVTGSSPVTTQVPHAVGIALAGKMEKKDLVTFVTFGEGSSNQGDFHEGANFAGVHKLPVI
EEECCCCCCCCCCHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCHHCCCCCCCEECCCEE
FMCENNKYAISIPVEKQLACKNVSDRAIGYGMPGYTVDGNDPLAVYKAVKEAADRGRRGE
EEECCCCEEEECCCHHHHHHCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHCCCCCC
GPTLIETVSYRLTAHSSDDDDRVYRDKEEVEEAKKNDSIVTFAAYLKEVGVLTEESEKQM
CCHHHHHHEEEEEECCCCCCHHHHCCHHHHHHHHCCCCEEHHHHHHHHHCCCCCHHHHHH
LDEIMHIVNEATEYAENAPYAAPEDALKHVYAE
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8504804; 8969508; 9384377 [H]