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The map label for this gene is bfmBAB [H]

Identifier: 49187064

GI number: 49187064

Start: 3997169

End: 3998152

Strand: Reverse

Name: bfmBAB [H]

Synonym: BAS4066

Alternate gene names: 49187064

Gene position: 3998152-3997169 (Counterclockwise)

Preceding gene: 49187065

Following gene: 49187063

Centisome position: 76.47

GC content: 39.63

Gene sequence:

>984_bases
ATGGCTGTAATGTCTTATATTGATGCTATTACATTAGCAATGCGCGAGGAAATGGAACGCGATGAGAAAGTATTCGTTTT
AGGTGAAGATGTTGGTAAAAAAGGTGGCGTATTTAAAGCGACACACGGATTATATGATCAATTTGGTGAAGATCGTGCGC
TAGATACACCACTTGCAGAATCTGCAATCGCTGGGGTCGCAATTGGTGCAGCAATGTATGGAATGCGTCCAATCGCTGAA
ATGCAGTTTGCGGATTTCATCATGCCAGCAGTAAACCAAATTGTTTCTGAGGCAGCAAAAATTCGTTACCGTTCTAATAA
TGATTGGACTTGTCCAATTACAGTTCGTGCGCCATTTGGCGGCGGTGTTCACGGAGCGTTGTATCATTCACAATCTGTAG
AAGCGATGTTTGCAAACCAACCTGGTTTAAAAATCGTTATCCCTTCTACACCATATGATGCAAAAGGCTTACTAAAAGCT
GCAATTCGTGATGAAGATCCAGTATTATTCTTTGAGCATAAACGTGCATATCGCTTAATTAAAGGTGAAGTGCCAGAAGA
TGATTACGTATTACCAATCGGAAAAGCGGATGTAAAACGTGAAGGTGATGATATTACTGTTATCACATACGGATTATGTG
TTCATTTTGCTCTTCAAGCAGCTGAAAAGTTAGCACAAGATGGCATCTCTGCACACATTCTTGATTTACGTACTGTATAT
CCATTAGATAAAGAAGCAATCATTGAAGCAGCTTCTAAAACAGGTAAAGTTCTTCTTGTAACAGAAGACAATAAAGAAGG
AAGTATTATGAGTGAAGTGGCAGCAATTATTGCTGAAAACTGTCTGTTTGATTTAGATGCACCAATCGCACGTCTTGCGG
GTCCAGACGTTCCAGCAATGCCATATGCACCAACAATGGAAAAATTCTTTATGGTAAATCCAGATAAAGTTGAAAAAGCA
ATGCGTGAACTTGCGGAATTTTAA

Upstream 100 bases:

>100_bases
CATATCGTAAACGAAGCAACAGAATATGCAGAAAATGCTCCGTATGCAGCACCTGAAGATGCATTGAAGCACGTATACGC
AGAATAGGGGGGAACGTTTC

Downstream 100 bases:

>100_bases
TCGGGGGGACTATACATGGCTGTAGAAAATATTACAATGCCTCAGCTCGGGGAGAGCGTTACAGAGGGTACAATTAGTAA
ATGGCTCGTTAATGTTGGCG

Product: 3-methyl-2-oxobutanoate dehydrogenase subunit beta

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase E1 component beta chain; BCKDH E1-beta [H]

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MAVMSYIDAITLAMREEMERDEKVFVLGEDVGKKGGVFKATHGLYDQFGEDRALDTPLAESAIAGVAIGAAMYGMRPIAE
MQFADFIMPAVNQIVSEAAKIRYRSNNDWTCPITVRAPFGGGVHGALYHSQSVEAMFANQPGLKIVIPSTPYDAKGLLKA
AIRDEDPVLFFEHKRAYRLIKGEVPEDDYVLPIGKADVKREGDDITVITYGLCVHFALQAAEKLAQDGISAHILDLRTVY
PLDKEAIIEAASKTGKVLLVTEDNKEGSIMSEVAAIIAENCLFDLDAPIARLAGPDVPAMPYAPTMEKFFMVNPDKVEKA
MRELAEF

Sequences:

>Translated_327_residues
MAVMSYIDAITLAMREEMERDEKVFVLGEDVGKKGGVFKATHGLYDQFGEDRALDTPLAESAIAGVAIGAAMYGMRPIAE
MQFADFIMPAVNQIVSEAAKIRYRSNNDWTCPITVRAPFGGGVHGALYHSQSVEAMFANQPGLKIVIPSTPYDAKGLLKA
AIRDEDPVLFFEHKRAYRLIKGEVPEDDYVLPIGKADVKREGDDITVITYGLCVHFALQAAEKLAQDGISAHILDLRTVY
PLDKEAIIEAASKTGKVLLVTEDNKEGSIMSEVAAIIAENCLFDLDAPIARLAGPDVPAMPYAPTMEKFFMVNPDKVEKA
MRELAEF
>Mature_326_residues
AVMSYIDAITLAMREEMERDEKVFVLGEDVGKKGGVFKATHGLYDQFGEDRALDTPLAESAIAGVAIGAAMYGMRPIAEM
QFADFIMPAVNQIVSEAAKIRYRSNNDWTCPITVRAPFGGGVHGALYHSQSVEAMFANQPGLKIVIPSTPYDAKGLLKAA
IRDEDPVLFFEHKRAYRLIKGEVPEDDYVLPIGKADVKREGDDITVITYGLCVHFALQAAEKLAQDGISAHILDLRTVYP
LDKEAIIEAASKTGKVLLVTEDNKEGSIMSEVAAIIAENCLFDLDAPIARLAGPDVPAMPYAPTMEKFFMVNPDKVEKAM
RELAEF

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4557353, Length=329, Percent_Identity=42.5531914893617, Blast_Score=273, Evalue=1e-73,
Organism=Homo sapiens, GI34101272, Length=329, Percent_Identity=42.5531914893617, Blast_Score=273, Evalue=1e-73,
Organism=Homo sapiens, GI156564403, Length=304, Percent_Identity=40.7894736842105, Blast_Score=236, Evalue=2e-62,
Organism=Homo sapiens, GI291084858, Length=304, Percent_Identity=39.8026315789474, Blast_Score=221, Evalue=9e-58,
Organism=Homo sapiens, GI133778974, Length=173, Percent_Identity=31.7919075144509, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI205277463, Length=298, Percent_Identity=25.503355704698, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI4507521, Length=298, Percent_Identity=25.503355704698, Blast_Score=67, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17538422, Length=326, Percent_Identity=42.0245398773006, Blast_Score=257, Evalue=5e-69,
Organism=Caenorhabditis elegans, GI17506935, Length=327, Percent_Identity=42.8134556574924, Blast_Score=249, Evalue=1e-66,
Organism=Saccharomyces cerevisiae, GI6319698, Length=327, Percent_Identity=40.0611620795107, Blast_Score=243, Evalue=3e-65,
Organism=Drosophila melanogaster, GI160714832, Length=326, Percent_Identity=42.3312883435583, Blast_Score=256, Evalue=2e-68,
Organism=Drosophila melanogaster, GI160714828, Length=326, Percent_Identity=42.3312883435583, Blast_Score=256, Evalue=2e-68,
Organism=Drosophila melanogaster, GI21358145, Length=308, Percent_Identity=41.2337662337662, Blast_Score=243, Evalue=1e-64,
Organism=Drosophila melanogaster, GI24650940, Length=308, Percent_Identity=41.2337662337662, Blast_Score=243, Evalue=1e-64,
Organism=Drosophila melanogaster, GI24650943, Length=90, Percent_Identity=41.1111111111111, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24650945, Length=90, Percent_Identity=41.1111111111111, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.4 [H]

Molecular weight: Translated: 35792; Mature: 35660

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVMSYIDAITLAMREEMERDEKVFVLGEDVGKKGGVFKATHGLYDQFGEDRALDTPLAE
CHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEHHHHHHHHCCCCCCCCCHHH
SAIAGVAIGAAMYGMRPIAEMQFADFIMPAVNQIVSEAAKIRYRSNNDWTCPITVRAPFG
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEEEEECCCC
GGVHGALYHSQSVEAMFANQPGLKIVIPSTPYDAKGLLKAAIRDEDPVLFFEHKRAYRLI
CCCCHHHCCCCCHHEEECCCCCCEEEECCCCCCHHHHHHHHHCCCCCEEEEECCHHHHHH
KGEVPEDDYVLPIGKADVKREGDDITVITYGLCVHFALQAAEKLAQDGISAHILDLRTVY
CCCCCCCCEEEECCCCHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHCC
PLDKEAIIEAASKTGKVLLVTEDNKEGSIMSEVAAIIAENCLFDLDAPIARLAGPDVPAM
CCCHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCC
PYAPTMEKFFMVNPDKVEKAMRELAEF
CCCCCCHHEEEECHHHHHHHHHHHHCC
>Mature Secondary Structure 
AVMSYIDAITLAMREEMERDEKVFVLGEDVGKKGGVFKATHGLYDQFGEDRALDTPLAE
HHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEHHHHHHHHCCCCCCCCCHHH
SAIAGVAIGAAMYGMRPIAEMQFADFIMPAVNQIVSEAAKIRYRSNNDWTCPITVRAPFG
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEEEEECCCC
GGVHGALYHSQSVEAMFANQPGLKIVIPSTPYDAKGLLKAAIRDEDPVLFFEHKRAYRLI
CCCCHHHCCCCCHHEEECCCCCCEEEECCCCCCHHHHHHHHHCCCCCEEEEECCHHHHHH
KGEVPEDDYVLPIGKADVKREGDDITVITYGLCVHFALQAAEKLAQDGISAHILDLRTVY
CCCCCCCCEEEECCCCHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHCC
PLDKEAIIEAASKTGKVLLVTEDNKEGSIMSEVAAIIAENCLFDLDAPIARLAGPDVPAM
CCCHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCC
PYAPTMEKFFMVNPDKVEKAMRELAEF
CCCCCCHHEEEECHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8504804; 8969508; 9384377 [H]