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The map label for this gene is bfmBB [H]

Identifier: 49187063

GI number: 49187063

Start: 3995834

End: 3997153

Strand: Reverse

Name: bfmBB [H]

Synonym: BAS4065

Alternate gene names: 49187063

Gene position: 3997153-3995834 (Counterclockwise)

Preceding gene: 49187064

Following gene: 49187062

Centisome position: 76.45

GC content: 40.3

Gene sequence:

>1320_bases
ATGGCTGTAGAAAATATTACAATGCCTCAGCTCGGGGAGAGCGTTACAGAGGGTACAATTAGTAAATGGCTCGTTAATGT
TGGCGATCACGTAAACAAATATGATCCGCTTGCAGAAGTAATGACTGATAAAGTAAATGCTGAAGTACCATCTTCTTTCA
CTGGTATTGTGAAAGAGTTGATCGCTGGTGAAGGTGATACGTTAGCTGTAGGTGAAGTAGTTTGTGTGATTCAAGTAGAA
GGCGCAGATGAAGTAGCAGCAACAGCTGTTGAGGAAAAAACAAAAGAAGAACCAAAAGCAGAAGTAAATACGCCTGAAAA
AGCACCGAAAGCAAAACAACCAACTGATGGAAAACCTCGTTTTTCACCAGCTGTGTTAAAACTTGCAGGTGAACATAACG
TTGATTTAGATCTAGTAGAAGGTACGGGAGCAAACGGCCGTATCACTCGTAAAGATATTTTAAAGCTAGTGGAATCAGGA
AATATTCCGCAAGCAGGTGCGGTGAAGAAAGAGGAAGCGGTAGCGGCAGTAGTAGAAGCGCGTCCAGAAGCACCAAAAGC
AGCGCCAGTAGCACAAAAAGTAGAAGCTGCGAAACCAGTTTCTGTACCAACAATGCCTGGCGATATCGAAATTCCAGTAA
CAGGTGTGCGTAAAGCAATCGCAGCGAACATGTTACGTAGTAAACACGAAGCGCCACACGCTTGGATGATGATTGAAGTA
GATGTGACAAACCTTGTGTCATACCGTAATTCAATTAAAGGTGATTTCAAAAAACGCGAAGGCTTTAACTTAACGTTCTT
TGCTTTCTTCGTAAAAGCAGTAGCACAAGCGTTAAAAGAGTACCCTCAAATTAATTCAATGTGGGCTGGCGATAAAATCG
TTCAGAAGAAAGATATTAACCTTTCTATCGCCGTTGCGACAGAGGACGAACTATTTGTACCAGTAATTAAACACGCGGAC
GAGAAGACAATTAAAGGTATCGCTCGTGAAATTACAGAACTTGCAGGAAAAGTACGTACGAAATCGTTAAAAGCGGACGA
AATGCAAGGCGGAACATTTACAATTAATAACACAGGATCATTCGGTTCTGTTCAATCTATGGGTATTATTAATTACCCAC
AAGCGGCTATTTTACAAGTTGAATCAATTGTAAAACGTCCAGTAATTATGGAAAATGGTATGTTCGGTGCTCGCGACATG
GTTAACTTATGTTTATCACTCGATCACCGTGTACTAGATGGTTTAATTTGTGGTAAGTTCTTAGGACGTGTAAAAGAAAT
TTTAGAAAATACGTCAGAAAATAATACATCTGTATATTAA

Upstream 100 bases:

>100_bases
GCCATATGCACCAACAATGGAAAAATTCTTTATGGTAAATCCAGATAAAGTTGAAAAAGCAATGCGTGAACTTGCGGAAT
TTTAATCGGGGGGACTATAC

Downstream 100 bases:

>100_bases
ATAAAAAGCTCGCTTATGCGAGCTTTTTATCATCTTATTTCTTCAACTACATCCTACTATATGTATAATACCTTTCCCAG
TAATCTTACAATGAACGATT

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase complex component E2; BCKAD-E2; BCKADE2; Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex; Dihydrolipoamide branched chain transacylase; Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase [H]

Number of amino acids: Translated: 439; Mature: 438

Protein sequence:

>439_residues
MAVENITMPQLGESVTEGTISKWLVNVGDHVNKYDPLAEVMTDKVNAEVPSSFTGIVKELIAGEGDTLAVGEVVCVIQVE
GADEVAATAVEEKTKEEPKAEVNTPEKAPKAKQPTDGKPRFSPAVLKLAGEHNVDLDLVEGTGANGRITRKDILKLVESG
NIPQAGAVKKEEAVAAVVEARPEAPKAAPVAQKVEAAKPVSVPTMPGDIEIPVTGVRKAIAANMLRSKHEAPHAWMMIEV
DVTNLVSYRNSIKGDFKKREGFNLTFFAFFVKAVAQALKEYPQINSMWAGDKIVQKKDINLSIAVATEDELFVPVIKHAD
EKTIKGIAREITELAGKVRTKSLKADEMQGGTFTINNTGSFGSVQSMGIINYPQAAILQVESIVKRPVIMENGMFGARDM
VNLCLSLDHRVLDGLICGKFLGRVKEILENTSENNTSVY

Sequences:

>Translated_439_residues
MAVENITMPQLGESVTEGTISKWLVNVGDHVNKYDPLAEVMTDKVNAEVPSSFTGIVKELIAGEGDTLAVGEVVCVIQVE
GADEVAATAVEEKTKEEPKAEVNTPEKAPKAKQPTDGKPRFSPAVLKLAGEHNVDLDLVEGTGANGRITRKDILKLVESG
NIPQAGAVKKEEAVAAVVEARPEAPKAAPVAQKVEAAKPVSVPTMPGDIEIPVTGVRKAIAANMLRSKHEAPHAWMMIEV
DVTNLVSYRNSIKGDFKKREGFNLTFFAFFVKAVAQALKEYPQINSMWAGDKIVQKKDINLSIAVATEDELFVPVIKHAD
EKTIKGIAREITELAGKVRTKSLKADEMQGGTFTINNTGSFGSVQSMGIINYPQAAILQVESIVKRPVIMENGMFGARDM
VNLCLSLDHRVLDGLICGKFLGRVKEILENTSENNTSVY
>Mature_438_residues
AVENITMPQLGESVTEGTISKWLVNVGDHVNKYDPLAEVMTDKVNAEVPSSFTGIVKELIAGEGDTLAVGEVVCVIQVEG
ADEVAATAVEEKTKEEPKAEVNTPEKAPKAKQPTDGKPRFSPAVLKLAGEHNVDLDLVEGTGANGRITRKDILKLVESGN
IPQAGAVKKEEAVAAVVEARPEAPKAAPVAQKVEAAKPVSVPTMPGDIEIPVTGVRKAIAANMLRSKHEAPHAWMMIEVD
VTNLVSYRNSIKGDFKKREGFNLTFFAFFVKAVAQALKEYPQINSMWAGDKIVQKKDINLSIAVATEDELFVPVIKHADE
KTIKGIAREITELAGKVRTKSLKADEMQGGTFTINNTGSFGSVQSMGIINYPQAAILQVESIVKRPVIMENGMFGARDMV
NLCLSLDHRVLDGLICGKFLGRVKEILENTSENNTSVY

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI203098816, Length=452, Percent_Identity=30.7522123893805, Blast_Score=176, Evalue=5e-44,
Organism=Homo sapiens, GI203098753, Length=452, Percent_Identity=30.7522123893805, Blast_Score=176, Evalue=6e-44,
Organism=Homo sapiens, GI31711992, Length=449, Percent_Identity=31.1804008908686, Blast_Score=175, Evalue=7e-44,
Organism=Homo sapiens, GI110671329, Length=438, Percent_Identity=30.5936073059361, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI19923748, Length=217, Percent_Identity=36.8663594470046, Blast_Score=132, Evalue=7e-31,
Organism=Homo sapiens, GI260898739, Length=157, Percent_Identity=36.9426751592357, Blast_Score=94, Evalue=3e-19,
Organism=Escherichia coli, GI1786946, Length=430, Percent_Identity=33.0232558139535, Blast_Score=214, Evalue=7e-57,
Organism=Escherichia coli, GI1786305, Length=443, Percent_Identity=32.9571106094808, Blast_Score=167, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17537937, Length=435, Percent_Identity=28.735632183908, Blast_Score=182, Evalue=4e-46,
Organism=Caenorhabditis elegans, GI25146366, Length=430, Percent_Identity=29.0697674418605, Blast_Score=162, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI17560088, Length=455, Percent_Identity=27.6923076923077, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17538894, Length=314, Percent_Identity=28.0254777070064, Blast_Score=119, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6320352, Length=426, Percent_Identity=30.9859154929577, Blast_Score=172, Evalue=7e-44,
Organism=Saccharomyces cerevisiae, GI6324258, Length=460, Percent_Identity=26.5217391304348, Blast_Score=139, Evalue=7e-34,
Organism=Drosophila melanogaster, GI18859875, Length=434, Percent_Identity=30.4147465437788, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI20129315, Length=225, Percent_Identity=33.3333333333333, Blast_Score=144, Evalue=9e-35,
Organism=Drosophila melanogaster, GI24582497, Length=225, Percent_Identity=33.3333333333333, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24645909, Length=215, Percent_Identity=34.8837209302326, Blast_Score=130, Evalue=3e-30,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.168 [H]

Molecular weight: Translated: 47411; Mature: 47280

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVENITMPQLGESVTEGTISKWLVNVGDHVNKYDPLAEVMTDKVNAEVPSSFTGIVKEL
CCCCCCCCHHHCCHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHHCCCCCCCHHHHHHHHH
IAGEGDTLAVGEVVCVIQVEGADEVAATAVEEKTKEEPKAEVNTPEKAPKAKQPTDGKPR
HCCCCCEEEECEEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
FSPAVLKLAGEHNVDLDLVEGTGANGRITRKDILKLVESGNIPQAGAVKKEEAVAAVVEA
CCHHHHEECCCCCCCEEEEECCCCCCEECHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHC
RPEAPKAAPVAQKVEAAKPVSVPTMPGDIEIPVTGVRKAIAANMLRSKHEAPHAWMMIEV
CCCCCCCCHHHHHHHHCCCCCCCCCCCCEECCHHHHHHHHHHHHHHHHCCCCCEEEEEEE
DVTNLVSYRNSIKGDFKKREGFNLTFFAFFVKAVAQALKEYPQINSMWAGDKIVQKKDIN
EHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHEEEECCC
LSIAVATEDELFVPVIKHADEKTIKGIAREITELAGKVRTKSLKADEMQGGTFTINNTGS
EEEEEECCCCEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEECCCCC
FGSVQSMGIINYPQAAILQVESIVKRPVIMENGMFGARDMVNLCLSLDHRVLDGLICGKF
CCCHHCCCCCCCCHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LGRVKEILENTSENNTSVY
HHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
AVENITMPQLGESVTEGTISKWLVNVGDHVNKYDPLAEVMTDKVNAEVPSSFTGIVKEL
CCCCCCCHHHCCHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHHCCCCCCCHHHHHHHHH
IAGEGDTLAVGEVVCVIQVEGADEVAATAVEEKTKEEPKAEVNTPEKAPKAKQPTDGKPR
HCCCCCEEEECEEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
FSPAVLKLAGEHNVDLDLVEGTGANGRITRKDILKLVESGNIPQAGAVKKEEAVAAVVEA
CCHHHHEECCCCCCCEEEEECCCCCCEECHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHC
RPEAPKAAPVAQKVEAAKPVSVPTMPGDIEIPVTGVRKAIAANMLRSKHEAPHAWMMIEV
CCCCCCCCHHHHHHHHCCCCCCCCCCCCEECCHHHHHHHHHHHHHHHHCCCCCEEEEEEE
DVTNLVSYRNSIKGDFKKREGFNLTFFAFFVKAVAQALKEYPQINSMWAGDKIVQKKDIN
EHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHEEEECCC
LSIAVATEDELFVPVIKHADEKTIKGIAREITELAGKVRTKSLKADEMQGGTFTINNTGS
EEEEEECCCCEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEECCCCC
FGSVQSMGIINYPQAAILQVESIVKRPVIMENGMFGARDMVNLCLSLDHRVLDGLICGKF
CCCHHCCCCCCCCHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LGRVKEILENTSENNTSVY
HHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8504804; 8969508; 9384377; 7961792 [H]