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The map label for this gene is murF [H]

Identifier: 49185041

GI number: 49185041

Start: 2038563

End: 2039294

Strand: Direct

Name: murF [H]

Synonym: BAS2030

Alternate gene names: 49185041

Gene position: 2038563-2039294 (Clockwise)

Preceding gene: 49185039

Following gene: 49185042

Centisome position: 38.99

GC content: 33.61

Gene sequence:

>732_bases
ATGAATTTGAAAAAATTTGAAGAGGTAATAAATGGAGAAGGATTACACGAATCGTTTCATCATTTAGAAATACATGGTGT
CTGCATAGATTCCAAAAACATTAAAGAAGGAAATGTATTCGTTCCAATCATTAGGGTGAAGGATGGGCATGAGTATGTGA
AAGAGGCAATGGATAATGGAGCTATCGCTTCCTTATGGAAAAAGTCTTACGGTTCTCCGCCAAAAGGGATTCCAATTATA
TTTGTAGATGACACTTTGTTTGCTTTGCAACAATTGGCACAATTTTATCGAAGAGAGATAAATGTAAAAGTAATCGGAAT
TACCGGTAGTAATGGAAAAACTACGGTAAAAGATATAGTAGAAGGTATTTTAAGGTCAACTTATAGAGTGCATAAAACAA
AAGGTAATTTTAATAGTCAAATCGGTTTACCGTTAACAATTTTAGAGATGAAACGAGATACAGAGATTTTAATACTTGAA
ATGGGAATGAGTGAAAAAGGATGTCGTATTATTGAAAGGTTCACGTGGAATAGCTTTAGAGGAAATTGTCCAAAAGTGGA
TGTGAAAAATCAAAATTTATTTAAAAATGCTTGCATTTTACAAAGTGAAGAGATAGAATACTCTGTAAATATAAAAAGTG
ATGATCAGGAAAAGTACATGATGCAAAGATCTACAGAGAGCAATCGGTTGCTGGAAAGATTGCGATACCGCATGATGGAA
AGACACCTGTGA

Upstream 100 bases:

>100_bases
CTTTTTTCATATAAACACCTCACTATAAAATTTTTATTAATATGCGTAAATAAAACTGAAAATATGTAATAAAATGGAAA
TAGGTGATAATTTGATTCAA

Downstream 100 bases:

>100_bases
GTGTTGCTTTGAACGTGATAATTAGTAAAAGCAAACGGTACCTACCGTTATCAGGACTAAGATGGTCATTATGACAATTT
GGGTGGTACCGCGGAAAAAT

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl-D-alanyl ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MNLKKFEEVINGEGLHESFHHLEIHGVCIDSKNIKEGNVFVPIIRVKDGHEYVKEAMDNGAIASLWKKSYGSPPKGIPII
FVDDTLFALQQLAQFYRREINVKVIGITGSNGKTTVKDIVEGILRSTYRVHKTKGNFNSQIGLPLTILEMKRDTEILILE
MGMSEKGCRIIERFTWNSFRGNCPKVDVKNQNLFKNACILQSEEIEYSVNIKSDDQEKYMMQRSTESNRLLERLRYRMME
RHL

Sequences:

>Translated_243_residues
MNLKKFEEVINGEGLHESFHHLEIHGVCIDSKNIKEGNVFVPIIRVKDGHEYVKEAMDNGAIASLWKKSYGSPPKGIPII
FVDDTLFALQQLAQFYRREINVKVIGITGSNGKTTVKDIVEGILRSTYRVHKTKGNFNSQIGLPLTILEMKRDTEILILE
MGMSEKGCRIIERFTWNSFRGNCPKVDVKNQNLFKNACILQSEEIEYSVNIKSDDQEKYMMQRSTESNRLLERLRYRMME
RHL
>Mature_243_residues
MNLKKFEEVINGEGLHESFHHLEIHGVCIDSKNIKEGNVFVPIIRVKDGHEYVKEAMDNGAIASLWKKSYGSPPKGIPII
FVDDTLFALQQLAQFYRREINVKVIGITGSNGKTTVKDIVEGILRSTYRVHKTKGNFNSQIGLPLTILEMKRDTEILILE
MGMSEKGCRIIERFTWNSFRGNCPKVDVKNQNLFKNACILQSEEIEYSVNIKSDDQEKYMMQRSTESNRLLERLRYRMME
RHL

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=168, Percent_Identity=28.5714285714286, Blast_Score=86, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 28084; Mature: 28084

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLKKFEEVINGEGLHESFHHLEIHGVCIDSKNIKEGNVFVPIIRVKDGHEYVKEAMDNG
CCHHHHHHHHCCCCCCCHHHEEEEEEEEECCCCCCCCCEEEEEEEEECCHHHHHHHHCCC
AIASLWKKSYGSPPKGIPIIFVDDTLFALQQLAQFYRREINVKVIGITGSNGKTTVKDIV
HHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHH
EGILRSTYRVHKTKGNFNSQIGLPLTILEMKRDTEILILEMGMSEKGCRIIERFTWNSFR
HHHHHHHHEEEECCCCCCCCCCCCEEEEEECCCCEEEEEEECCCCCCCHHHHHHCCCCCC
GNCPKVDVKNQNLFKNACILQSEEIEYSVNIKSDDQEKYMMQRSTESNRLLERLRYRMME
CCCCEEECCCCCHHHHHHEEECCCEEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHH
RHL
HCC
>Mature Secondary Structure
MNLKKFEEVINGEGLHESFHHLEIHGVCIDSKNIKEGNVFVPIIRVKDGHEYVKEAMDNG
CCHHHHHHHHCCCCCCCHHHEEEEEEEEECCCCCCCCCEEEEEEEEECCHHHHHHHHCCC
AIASLWKKSYGSPPKGIPIIFVDDTLFALQQLAQFYRREINVKVIGITGSNGKTTVKDIV
HHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHH
EGILRSTYRVHKTKGNFNSQIGLPLTILEMKRDTEILILEMGMSEKGCRIIERFTWNSFR
HHHHHHHHEEEECCCCCCCCCCCCEEEEEECCCCEEEEEEECCCCCCCHHHHHHCCCCCC
GNCPKVDVKNQNLFKNACILQSEEIEYSVNIKSDDQEKYMMQRSTESNRLLERLRYRMME
CCCCEEECCCCCHHHHHHEEECCCEEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHH
RHL
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]