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The map label for this gene is aspC [H]

Identifier: 49185042

GI number: 49185042

Start: 2039514

End: 2040812

Strand: Direct

Name: aspC [H]

Synonym: BAS2031

Alternate gene names: 49185042

Gene position: 2039514-2040812 (Clockwise)

Preceding gene: 49185041

Following gene: 49185043

Centisome position: 39.01

GC content: 35.1

Gene sequence:

>1299_bases
ATGGGGCAAATCATGAAACGTTCACTCACAAAAGAATGTACGGAGAAAAGCGGAAAGGTTGTATCACTTCAAGGCTGGGT
AAAAAAGATTCGTCATCTAGGTAATGTTAGTTTTTTATTATTACGGGACAGAACAGGAGTCATTCAATGTGTATTAGAAA
ACGAATTCGCTGGATTCAAAGTTGATGTAGAAAGTATTGTTCACGTAATTGGTGAAATAGTTGAAACGTCAAAAACAGAA
TTAGGCGTAGAAGTACTTGCTCACGAAGTGAAAATTTTAAATGGAGCAGAGCCTCTTCCGTTTGAAATAAATAAAAAGAA
GTTACAAGTTGGTTTAGATCAACTACTTAATGAGCGAGTATTATCATTAAGACATGAGCGAACCACGGCGATATTTAAAG
TGAAATCTACACTCGTTCAGAGCTTTAGTGAATTACTTATAGAGAACGATTTCACACGTATATTTACTCCGAAAATTGTT
TCACAAGGGGCAGAAGGAGGAGCGAATGTTTTTAAGCTTCCATACTTCCAAAAAGAAGCTTATTTAGCACAATCACCACA
GTTTTATAAACAAATGATGGTAGCTGGGGGACTCGAACGTGTTTTTGAAATTGCACCTGTTTATAGGGCGGAACATCACA
ATTCTTCCCGTCATTTAAATGAATATATATCGTTAGACGTAGAGCTTGGCTTTATTCATGATTTTCATGAAGTGATGCAA
TTAGAAACAGATGTTTTACGATATATGTTTCAACAAGTAGCAAAAAACTGTGAGAAAGAACTACAACTATTACAAATAGA
AGTACCTGTTATTACTGAAATACCGAAAATCACATTGTTAGAAGCACAAGAAATCTTGAAAAGTAAGTATTGTAAAGAAT
CTCCTGTAGGGGATTTAGATACAGAAGGTGAAAAGTTACTAGGGAAATATGTGAAGGAAACATATAAGAGTGAATTTGTT
TTTATTACACATTATCCGAAAGAAGCGAGACCGATGTATACGATGCCGAATAACGAGAATTCGGCTATTACTGATTCGTT
CGACTTACTATATAAAGGATTAGAAATAACATCAGGAGCACAGCGAATTCATAATTATGAAATGTTACTAGATTCTTTTA
AAGAAAAAGGATTACATCCCGATAAATTTCAATCGTATTTAAATACATTCCGATACGGTTGTCCCCCGCACGGTGGTTTT
GGAATTGGCTTAGAGAGAATTGTATATAAACTTTTAGAATTATCGAATGTGAGAGAAGCGAGTGCTTTTCCGAGAGATTG
TACGCGTCTTATACCGTAA

Upstream 100 bases:

>100_bases
CGGATTTTTGTATTCTTTGAAAGGGGGTGAGTGACCGTGGATGATGACGATGACAACAACAATAACGTAAATAAATATAA
AATTACTGGAGGGAAATGTA

Downstream 100 bases:

>100_bases
CATTAGCACCCTTATATAGTATAGAAGGGTGCTTTGCATTTCAATAAAAGTGAAAGAGAATAATAATATTTTTTGTGTGA
AAATATGTAAATTATATTAA

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MGQIMKRSLTKECTEKSGKVVSLQGWVKKIRHLGNVSFLLLRDRTGVIQCVLENEFAGFKVDVESIVHVIGEIVETSKTE
LGVEVLAHEVKILNGAEPLPFEINKKKLQVGLDQLLNERVLSLRHERTTAIFKVKSTLVQSFSELLIENDFTRIFTPKIV
SQGAEGGANVFKLPYFQKEAYLAQSPQFYKQMMVAGGLERVFEIAPVYRAEHHNSSRHLNEYISLDVELGFIHDFHEVMQ
LETDVLRYMFQQVAKNCEKELQLLQIEVPVITEIPKITLLEAQEILKSKYCKESPVGDLDTEGEKLLGKYVKETYKSEFV
FITHYPKEARPMYTMPNNENSAITDSFDLLYKGLEITSGAQRIHNYEMLLDSFKEKGLHPDKFQSYLNTFRYGCPPHGGF
GIGLERIVYKLLELSNVREASAFPRDCTRLIP

Sequences:

>Translated_432_residues
MGQIMKRSLTKECTEKSGKVVSLQGWVKKIRHLGNVSFLLLRDRTGVIQCVLENEFAGFKVDVESIVHVIGEIVETSKTE
LGVEVLAHEVKILNGAEPLPFEINKKKLQVGLDQLLNERVLSLRHERTTAIFKVKSTLVQSFSELLIENDFTRIFTPKIV
SQGAEGGANVFKLPYFQKEAYLAQSPQFYKQMMVAGGLERVFEIAPVYRAEHHNSSRHLNEYISLDVELGFIHDFHEVMQ
LETDVLRYMFQQVAKNCEKELQLLQIEVPVITEIPKITLLEAQEILKSKYCKESPVGDLDTEGEKLLGKYVKETYKSEFV
FITHYPKEARPMYTMPNNENSAITDSFDLLYKGLEITSGAQRIHNYEMLLDSFKEKGLHPDKFQSYLNTFRYGCPPHGGF
GIGLERIVYKLLELSNVREASAFPRDCTRLIP
>Mature_431_residues
GQIMKRSLTKECTEKSGKVVSLQGWVKKIRHLGNVSFLLLRDRTGVIQCVLENEFAGFKVDVESIVHVIGEIVETSKTEL
GVEVLAHEVKILNGAEPLPFEINKKKLQVGLDQLLNERVLSLRHERTTAIFKVKSTLVQSFSELLIENDFTRIFTPKIVS
QGAEGGANVFKLPYFQKEAYLAQSPQFYKQMMVAGGLERVFEIAPVYRAEHHNSSRHLNEYISLDVELGFIHDFHEVMQL
ETDVLRYMFQQVAKNCEKELQLLQIEVPVITEIPKITLLEAQEILKSKYCKESPVGDLDTEGEKLLGKYVKETYKSEFVF
ITHYPKEARPMYTMPNNENSAITDSFDLLYKGLEITSGAQRIHNYEMLLDSFKEKGLHPDKFQSYLNTFRYGCPPHGGFG
IGLERIVYKLLELSNVREASAFPRDCTRLIP

Specific function: Unknown

COG id: COG0017

COG function: function code J; Aspartyl/asparaginyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI45439306, Length=456, Percent_Identity=36.1842105263158, Blast_Score=292, Evalue=5e-79,
Organism=Homo sapiens, GI4758762, Length=431, Percent_Identity=29.9303944315545, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI226491502, Length=438, Percent_Identity=27.3972602739726, Blast_Score=158, Evalue=1e-38,
Organism=Homo sapiens, GI40789249, Length=283, Percent_Identity=28.9752650176678, Blast_Score=93, Evalue=4e-19,
Organism=Escherichia coli, GI1787161, Length=450, Percent_Identity=27.1111111111111, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1790571, Length=450, Percent_Identity=25.5555555555556, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1789256, Length=448, Percent_Identity=25.2232142857143, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1788173, Length=252, Percent_Identity=25.7936507936508, Blast_Score=88, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17551876, Length=450, Percent_Identity=37.3333333333333, Blast_Score=290, Evalue=7e-79,
Organism=Caenorhabditis elegans, GI71984122, Length=445, Percent_Identity=27.4157303370787, Blast_Score=158, Evalue=6e-39,
Organism=Caenorhabditis elegans, GI17556372, Length=406, Percent_Identity=26.6009852216749, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI32566633, Length=251, Percent_Identity=29.4820717131474, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323011, Length=454, Percent_Identity=36.3436123348018, Blast_Score=277, Evalue=2e-75,
Organism=Saccharomyces cerevisiae, GI6321807, Length=442, Percent_Identity=30.316742081448, Blast_Score=186, Evalue=6e-48,
Organism=Saccharomyces cerevisiae, GI6319872, Length=481, Percent_Identity=27.027027027027, Blast_Score=138, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6325153, Length=242, Percent_Identity=27.6859504132231, Blast_Score=76, Evalue=9e-15,
Organism=Drosophila melanogaster, GI17136276, Length=455, Percent_Identity=36.4835164835165, Blast_Score=291, Evalue=6e-79,
Organism=Drosophila melanogaster, GI19921528, Length=436, Percent_Identity=28.2110091743119, Blast_Score=162, Evalue=3e-40,
Organism=Drosophila melanogaster, GI19922416, Length=446, Percent_Identity=22.6457399103139, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI24584738, Length=292, Percent_Identity=32.5342465753425, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020780
- InterPro:   IPR004523
- InterPro:   IPR002312
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 49403; Mature: 49272

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQIMKRSLTKECTEKSGKVVSLQGWVKKIRHLGNVSFLLLRDRTGVIQCVLENEFAGFK
CCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHCCCCEEEEEECCCCEEEEEECCCCCCCE
VDVESIVHVIGEIVETSKTELGVEVLAHEVKILNGAEPLPFEINKKKLQVGLDQLLNERV
ECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
LSLRHERTTAIFKVKSTLVQSFSELLIENDFTRIFTPKIVSQGAEGGANVFKLPYFQKEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHCCCCCCCCEEECCCHHHHH
YLAQSPQFYKQMMVAGGLERVFEIAPVYRAEHHNSSRHLNEYISLDVELGFIHDFHEVMQ
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHEEEEEEHHHHHHHHHHH
LETDVLRYMFQQVAKNCEKELQLLQIEVPVITEIPKITLLEAQEILKSKYCKESPVGDLD
HHHHHHHHHHHHHHHHHHHHHHHEEEECCHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCC
TEGEKLLGKYVKETYKSEFVFITHYPKEARPMYTMPNNENSAITDSFDLLYKGLEITSGA
CCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEECCCCCCCCCHHHHHHHHHCCHHCHHH
QRIHNYEMLLDSFKEKGLHPDKFQSYLNTFRYGCPPHGGFGIGLERIVYKLLELSNVREA
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCHHH
SAFPRDCTRLIP
CCCCHHHHHCCC
>Mature Secondary Structure 
GQIMKRSLTKECTEKSGKVVSLQGWVKKIRHLGNVSFLLLRDRTGVIQCVLENEFAGFK
CHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHCCCCEEEEEECCCCEEEEEECCCCCCCE
VDVESIVHVIGEIVETSKTELGVEVLAHEVKILNGAEPLPFEINKKKLQVGLDQLLNERV
ECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
LSLRHERTTAIFKVKSTLVQSFSELLIENDFTRIFTPKIVSQGAEGGANVFKLPYFQKEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHCCCCCCCCEEECCCHHHHH
YLAQSPQFYKQMMVAGGLERVFEIAPVYRAEHHNSSRHLNEYISLDVELGFIHDFHEVMQ
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHEEEEEEHHHHHHHHHHH
LETDVLRYMFQQVAKNCEKELQLLQIEVPVITEIPKITLLEAQEILKSKYCKESPVGDLD
HHHHHHHHHHHHHHHHHHHHHHHEEEECCHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCC
TEGEKLLGKYVKETYKSEFVFITHYPKEARPMYTMPNNENSAITDSFDLLYKGLEITSGA
CCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEECCCCCCCCCHHHHHHHHHCCHHCHHH
QRIHNYEMLLDSFKEKGLHPDKFQSYLNTFRYGCPPHGGFGIGLERIVYKLLELSNVREA
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCHHH
SAFPRDCTRLIP
CCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]