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The map label for this gene is ilvE [H]

Identifier: 49184726

GI number: 49184726

Start: 1731144

End: 1732043

Strand: Direct

Name: ilvE [H]

Synonym: BAS1713

Alternate gene names: 49184726

Gene position: 1731144-1732043 (Clockwise)

Preceding gene: 49184723

Following gene: 49184727

Centisome position: 33.11

GC content: 38.67

Gene sequence:

>900_bases
ATGGGAAACCAGTACATTTATATGAATGGGGAATTTGTAGAAAAAGAAAAGGCAGTTGTTTCAGTATATGATCACGGTTT
TTTATACGGAGACGGTGTATTTGAAGGAATTCGTAGTTACGGTGGAAATGTATTTTGTTTAAAAGAACATGTAAAACGAT
TGTATGAATCGGCAAAATCTATTTTACTTACAATCCCGCTGACGGTTGACGAAATGGAAGAAGCAGTTTTACAGACACTT
CAAAAAAATGAGTATGCAGATGCTTACATTCGCTTAATTGTTTCAAGAGGAAAAGGGGACTTAGGACTTGATCCGAGAAG
CTGTGTGAAACCGAGCGTTATTATCATTGCAGAACAATTAAAATTATTCCCTCAGGAATTTTACGATAACGGACTAAGTG
TTGTATCTGTTGCATCAAGACGTAATACGCCAGATGCATTAGACCCACGAATTAAGTCAATGAACTACTTAAATAACGTA
CTTGTAAAAATTGAAGCGGCACAAGCAGGAGTGCTAGAGGCACTTATGTTAAATCAGCAAGGATATGTTTGTGAAGGATC
TGGAGATAATGTTTTCGTTGTGAAAGATGGAAAAGTGTTAACACCTCCGTCATATTTAGGGGCATTAGAAGGCATTACGA
GAAATAGTGTTATCGAGCTATGTGAGCGACTGAGTATTCCGTGTGAGGAAAGACCATTCACTCGCCATGACGTATATGTA
GCGGATGAAGTGTTTTTAACAGGAACGGCAGCTGAATTAATTCCAGTTGTAAAGGTGGATTCAAGAGAAATTGGAGATGG
GAAACCAGGAAGTGTGACGAAACAATTGACTGAGGAATTTAAAAAGTTAACGAGAGAAAGAGGCGTACGTGTTCCAGGAC
TGGCAGAAAGCTTACTGTAG

Upstream 100 bases:

>100_bases
AAGATTTATATACTGAATATTCTGTTAAAAATACTCATCTCAGCAATCGATATAGAAATAGATTGCAAAAATAGAGAGAA
AAAAAGAGGAGCGATGTGAG

Downstream 100 bases:

>100_bases
GTAGGGAGAGGAGTTAATTGAAATGAAGCAATATACAACCTATGAGAAATTGCAGTGTGAAGAAGTGACAGGAGCTGGAC
ACGTTATTCAATGCTTAAAG

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT; Transaminase B [H]

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MGNQYIYMNGEFVEKEKAVVSVYDHGFLYGDGVFEGIRSYGGNVFCLKEHVKRLYESAKSILLTIPLTVDEMEEAVLQTL
QKNEYADAYIRLIVSRGKGDLGLDPRSCVKPSVIIIAEQLKLFPQEFYDNGLSVVSVASRRNTPDALDPRIKSMNYLNNV
LVKIEAAQAGVLEALMLNQQGYVCEGSGDNVFVVKDGKVLTPPSYLGALEGITRNSVIELCERLSIPCEERPFTRHDVYV
ADEVFLTGTAAELIPVVKVDSREIGDGKPGSVTKQLTEEFKKLTRERGVRVPGLAESLL

Sequences:

>Translated_299_residues
MGNQYIYMNGEFVEKEKAVVSVYDHGFLYGDGVFEGIRSYGGNVFCLKEHVKRLYESAKSILLTIPLTVDEMEEAVLQTL
QKNEYADAYIRLIVSRGKGDLGLDPRSCVKPSVIIIAEQLKLFPQEFYDNGLSVVSVASRRNTPDALDPRIKSMNYLNNV
LVKIEAAQAGVLEALMLNQQGYVCEGSGDNVFVVKDGKVLTPPSYLGALEGITRNSVIELCERLSIPCEERPFTRHDVYV
ADEVFLTGTAAELIPVVKVDSREIGDGKPGSVTKQLTEEFKKLTRERGVRVPGLAESLL
>Mature_298_residues
GNQYIYMNGEFVEKEKAVVSVYDHGFLYGDGVFEGIRSYGGNVFCLKEHVKRLYESAKSILLTIPLTVDEMEEAVLQTLQ
KNEYADAYIRLIVSRGKGDLGLDPRSCVKPSVIIIAEQLKLFPQEFYDNGLSVVSVASRRNTPDALDPRIKSMNYLNNVL
VKIEAAQAGVLEALMLNQQGYVCEGSGDNVFVVKDGKVLTPPSYLGALEGITRNSVIELCERLSIPCEERPFTRHDVYVA
DEVFLTGTAAELIPVVKVDSREIGDGKPGSVTKQLTEEFKKLTRERGVRVPGLAESLL

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=290, Percent_Identity=38.9655172413793, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI1787338, Length=241, Percent_Identity=25.3112033195021, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005785 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 33116; Mature: 32985

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNQYIYMNGEFVEKEKAVVSVYDHGFLYGDGVFEGIRSYGGNVFCLKEHVKRLYESAKS
CCCCEEEECCCEECCCCEEEEEECCCEEECCHHHHHHHHCCCCEEEHHHHHHHHHHHHCE
ILLTIPLTVDEMEEAVLQTLQKNEYADAYIRLIVSRGKGDLGLDPRSCVKPSVIIIAEQL
EEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHHCCCCEEEEEHHH
KLFPQEFYDNGLSVVSVASRRNTPDALDPRIKSMNYLNNVLVKIEAAQAGVLEALMLNQQ
HHHHHHHHCCCCEEEEEHHCCCCCCCCCHHHHHHHHHHHEEEEEECHHHHHHHHHHHCCC
GYVCEGSGDNVFVVKDGKVLTPPSYLGALEGITRNSVIELCERLSIPCEERPFTRHDVYV
CCEEECCCCEEEEEECCEEECCHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCEEE
ADEVFLTGTAAELIPVVKVDSREIGDGKPGSVTKQLTEEFKKLTRERGVRVPGLAESLL
ECEEEEECCHHHHEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHCC
>Mature Secondary Structure 
GNQYIYMNGEFVEKEKAVVSVYDHGFLYGDGVFEGIRSYGGNVFCLKEHVKRLYESAKS
CCCEEEECCCEECCCCEEEEEECCCEEECCHHHHHHHHCCCCEEEHHHHHHHHHHHHCE
ILLTIPLTVDEMEEAVLQTLQKNEYADAYIRLIVSRGKGDLGLDPRSCVKPSVIIIAEQL
EEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHHCCCCEEEEEHHH
KLFPQEFYDNGLSVVSVASRRNTPDALDPRIKSMNYLNNVLVKIEAAQAGVLEALMLNQQ
HHHHHHHHCCCCEEEEEHHCCCCCCCCCHHHHHHHHHHHEEEEEECHHHHHHHHHHHCCC
GYVCEGSGDNVFVVKDGKVLTPPSYLGALEGITRNSVIELCERLSIPCEERPFTRHDVYV
CCEEECCCCEEEEEECCEEECCHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCEEE
ADEVFLTGTAAELIPVVKVDSREIGDGKPGSVTKQLTEEFKKLTRERGVRVPGLAESLL
ECEEEEECCHHHHEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]