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The map label for this gene is ilvB [H]

Identifier: 49184727

GI number: 49184727

Start: 1732066

End: 1733778

Strand: Direct

Name: ilvB [H]

Synonym: BAS1714

Alternate gene names: 49184727

Gene position: 1732066-1733778 (Clockwise)

Preceding gene: 49184726

Following gene: 49184728

Centisome position: 33.13

GC content: 40.4

Gene sequence:

>1713_bases
ATGAAGCAATATACAACCTATGAGAAATTGCAGTGTGAAGAAGTGACAGGAGCTGGACACGTTATTCAATGCTTAAAGAA
ATTAGGCGTAACGACTGTTTTCGGTTATCCGGGCGGAGCAATTTTACCAGTGTACGATGCGCTATATGAAAGTGGATTGA
AGCATATATTAACTCGTCATGAACAAGCTGCTATTCATGCGGCTGAAGGTTATGCAAGAGCTTCTGGAAAGGTCGGGGTA
GTATTTGCTACCTCTGGCCCAGGGGCGACGAATTTAGTTACAGGCTTAGCAGATGCTTATATGGATTCGATTCCTTTAGT
TGTCATTACAGGGCAAGTTGCAACACCACTCATTGGAAAAGACGGGTTTCAAGAAGCTGATGTTGTCGGAATTACAGTAC
CTGTTACGAAGCATAATTACCAAGTTCGTGATGTGAATCAGTTATCACGTATTGTACAAGAAGCTTTTTACATCGCCGAA
AGCGGGCGGCCAGGACCAGTATTAATTGATATTCCAAAAGATGTTCAAATTGAAAAGGTAACAAGTTTTTACAATGAAGT
AATTGAGATTCCAGGATACAAACTAGAGCCTAGGCCAGATAGTATGAAGCTTAGAGAAGTGGCTAAAGCCATTTCAGAAG
CAAAACGCCCACTTCTTTATATCGGAGGAGGAATCATTCATGCAGATGGATCTGAAGAACTTATTCAGTTTGCGAGGAAG
AAGGGGATTCCTGTCGTTTCCACTTTAATGGGACTTGGTGCATATCCGCCGGGAGATCCATTATTTTTAGGAATGCTCGG
TATGCATGGAACGTACGCAGCAAACATGGCAGTAACAGAATGTGATTTACTGCTTGCGTTAGGTGTTCGTTTTGATGACC
GTGTAACAGGAAAACTGGAACTTTTTTCTCCGCATTCGAAAAAGGTACATATTGATATAGATCCTTCAGAGTTTCAAAAA
AATGTAGCGGTAGAGTATCCAGTTGTTGGTGATGTAAAAAAAGCATTACACATGCTGTTACATATGTCTATTCATACACA
AACAGACGAATGGCTTCAGAAAGTAAAGACATGGAAAGAAGAATATCAACTTTCTTATAAGCAAAAAGAATCTGAGTTAA
AACCACAACATGTCATCAACTTAGTAAGCGAATTAACGAATGGTGAAGCGATTGTGACGACAGAAGTAGGACAGCATCAA
ATGTGGGCTGCGCATTTTTATAAAGCGAAAAACCCGCGGACTTTTCTAACATCTGGTGGGTTAGGAACGATGGGATTCGG
TTTCCCAGCGGCAATAGGTGCTCAGCTTGCTAAAGAAGAAGAACTTGTCATTTGTATCGCGGGCGATGCTTCTTTTCAAA
TGAACATTCAAGAACTGCAAACAATTGCTGAAAATAACATCCCTGTAAAAGTATTTATAATAAACAACAAATTTTTAGGG
ATGGTAAGGCAATGGCAAGAAATGTTTTATGAAAACCGATTGTCAGAGTCGAAAATTGGATCGCCAGATTTTGTGAAAGT
GGCAGAAGCTTATGGGGTGAAAGGATTAAGAGCGACAAATGCAACCGAGGCGAAACAAGTGATGTTAGAAGCATTTGCTC
ATAAAGGCCCTGTCGTAGTTGATTTTTGTGTAGAAGAAGGTGAAAACGTATTTCCGATGGTTCCGCCAAATAAAGGGAAT
AACGAAATGATTATGAAGAGGTGGGAAGAATGA

Upstream 100 bases:

>100_bases
CAATTGACTGAGGAATTTAAAAAGTTAACGAGAGAAAGAGGCGTACGTGTTCCAGGACTGGCAGAAAGCTTACTGTAGGT
AGGGAGAGGAGTTAATTGAA

Downstream 100 bases:

>100_bases
GTCATACTTTTTCATTAGTTATCGATAACGATCCAAATGTCTTATTACGTATAAGTGGAATTTTTGCTCGGCGTGGTTAT
TATATTTCTTCATTAAATTT

Product: acetolactate synthase 3 catalytic subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS; Vegetative protein 105; VEG105 [H]

Number of amino acids: Translated: 570; Mature: 570

Protein sequence:

>570_residues
MKQYTTYEKLQCEEVTGAGHVIQCLKKLGVTTVFGYPGGAILPVYDALYESGLKHILTRHEQAAIHAAEGYARASGKVGV
VFATSGPGATNLVTGLADAYMDSIPLVVITGQVATPLIGKDGFQEADVVGITVPVTKHNYQVRDVNQLSRIVQEAFYIAE
SGRPGPVLIDIPKDVQIEKVTSFYNEVIEIPGYKLEPRPDSMKLREVAKAISEAKRPLLYIGGGIIHADGSEELIQFARK
KGIPVVSTLMGLGAYPPGDPLFLGMLGMHGTYAANMAVTECDLLLALGVRFDDRVTGKLELFSPHSKKVHIDIDPSEFQK
NVAVEYPVVGDVKKALHMLLHMSIHTQTDEWLQKVKTWKEEYQLSYKQKESELKPQHVINLVSELTNGEAIVTTEVGQHQ
MWAAHFYKAKNPRTFLTSGGLGTMGFGFPAAIGAQLAKEEELVICIAGDASFQMNIQELQTIAENNIPVKVFIINNKFLG
MVRQWQEMFYENRLSESKIGSPDFVKVAEAYGVKGLRATNATEAKQVMLEAFAHKGPVVVDFCVEEGENVFPMVPPNKGN
NEMIMKRWEE

Sequences:

>Translated_570_residues
MKQYTTYEKLQCEEVTGAGHVIQCLKKLGVTTVFGYPGGAILPVYDALYESGLKHILTRHEQAAIHAAEGYARASGKVGV
VFATSGPGATNLVTGLADAYMDSIPLVVITGQVATPLIGKDGFQEADVVGITVPVTKHNYQVRDVNQLSRIVQEAFYIAE
SGRPGPVLIDIPKDVQIEKVTSFYNEVIEIPGYKLEPRPDSMKLREVAKAISEAKRPLLYIGGGIIHADGSEELIQFARK
KGIPVVSTLMGLGAYPPGDPLFLGMLGMHGTYAANMAVTECDLLLALGVRFDDRVTGKLELFSPHSKKVHIDIDPSEFQK
NVAVEYPVVGDVKKALHMLLHMSIHTQTDEWLQKVKTWKEEYQLSYKQKESELKPQHVINLVSELTNGEAIVTTEVGQHQ
MWAAHFYKAKNPRTFLTSGGLGTMGFGFPAAIGAQLAKEEELVICIAGDASFQMNIQELQTIAENNIPVKVFIINNKFLG
MVRQWQEMFYENRLSESKIGSPDFVKVAEAYGVKGLRATNATEAKQVMLEAFAHKGPVVVDFCVEEGENVFPMVPPNKGN
NEMIMKRWEE
>Mature_570_residues
MKQYTTYEKLQCEEVTGAGHVIQCLKKLGVTTVFGYPGGAILPVYDALYESGLKHILTRHEQAAIHAAEGYARASGKVGV
VFATSGPGATNLVTGLADAYMDSIPLVVITGQVATPLIGKDGFQEADVVGITVPVTKHNYQVRDVNQLSRIVQEAFYIAE
SGRPGPVLIDIPKDVQIEKVTSFYNEVIEIPGYKLEPRPDSMKLREVAKAISEAKRPLLYIGGGIIHADGSEELIQFARK
KGIPVVSTLMGLGAYPPGDPLFLGMLGMHGTYAANMAVTECDLLLALGVRFDDRVTGKLELFSPHSKKVHIDIDPSEFQK
NVAVEYPVVGDVKKALHMLLHMSIHTQTDEWLQKVKTWKEEYQLSYKQKESELKPQHVINLVSELTNGEAIVTTEVGQHQ
MWAAHFYKAKNPRTFLTSGGLGTMGFGFPAAIGAQLAKEEELVICIAGDASFQMNIQELQTIAENNIPVKVFIINNKFLG
MVRQWQEMFYENRLSESKIGSPDFVKVAEAYGVKGLRATNATEAKQVMLEAFAHKGPVVVDFCVEEGENVFPMVPPNKGN
NEMIMKRWEE

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI21361361, Length=560, Percent_Identity=25.5357142857143, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI93004078, Length=560, Percent_Identity=25, Blast_Score=156, Evalue=4e-38,
Organism=Escherichia coli, GI87081685, Length=572, Percent_Identity=45.8041958041958, Blast_Score=522, Evalue=1e-149,
Organism=Escherichia coli, GI1790104, Length=562, Percent_Identity=43.5943060498221, Blast_Score=471, Evalue=1e-134,
Organism=Escherichia coli, GI1786717, Length=554, Percent_Identity=35.0180505415162, Blast_Score=319, Evalue=4e-88,
Organism=Escherichia coli, GI1787096, Length=546, Percent_Identity=27.4725274725275, Blast_Score=195, Evalue=6e-51,
Organism=Escherichia coli, GI1788716, Length=562, Percent_Identity=24.0213523131673, Blast_Score=150, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17531299, Length=550, Percent_Identity=28.1818181818182, Blast_Score=162, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI17531301, Length=550, Percent_Identity=28.1818181818182, Blast_Score=161, Evalue=9e-40,
Organism=Caenorhabditis elegans, GI17542570, Length=587, Percent_Identity=25.2129471890971, Blast_Score=160, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6323755, Length=574, Percent_Identity=46.6898954703833, Blast_Score=484, Evalue=1e-137,
Organism=Saccharomyces cerevisiae, GI6320816, Length=490, Percent_Identity=26.734693877551, Blast_Score=125, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6321524, Length=497, Percent_Identity=21.9315895372233, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19922626, Length=481, Percent_Identity=27.6507276507277, Blast_Score=171, Evalue=1e-42,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 62796; Mature: 62796

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQYTTYEKLQCEEVTGAGHVIQCLKKLGVTTVFGYPGGAILPVYDALYESGLKHILTRH
CCCCCCHHHHHHHHCCCCHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
EQAAIHAAEGYARASGKVGVVFATSGPGATNLVTGLADAYMDSIPLVVITGQVATPLIGK
HHHHHHHHCCHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCC
DGFQEADVVGITVPVTKHNYQVRDVNQLSRIVQEAFYIAESGRPGPVLIDIPKDVQIEKV
CCCCCCCEEEEEEEEECCCEEECCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHH
TSFYNEVIEIPGYKLEPRPDSMKLREVAKAISEAKRPLLYIGGGIIHADGSEELIQFARK
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCEEEECCCHHHHHHHHHH
KGIPVVSTLMGLGAYPPGDPLFLGMLGMHGTYAANMAVTECDLLLALGVRFDDRVTGKLE
CCCHHHHHHHHCCCCCCCCCEEEEHHCCCCCHHCCEEHHHHHHHHHHCCCCCCCCCCEEE
LFSPHSKKVHIDIDPSEFQKNVAVEYPVVGDVKKALHMLLHMSIHTQTDEWLQKVKTWKE
EECCCCCEEEEECCHHHHHCCCEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
EYQLSYKQKESELKPQHVINLVSELTNGEAIVTTEVGQHQMWAAHFYKAKNPRTFLTSGG
HHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCEEEECCC
LGTMGFGFPAAIGAQLAKEEELVICIAGDASFQMNIQELQTIAENNIPVKVFIINNKFLG
CCCCCCCCHHHHHHHHCCCCCEEEEEECCCCEEEEHHHHHHHHCCCCCEEEEEECCHHHH
MVRQWQEMFYENRLSESKIGSPDFVKVAEAYGVKGLRATNATEAKQVMLEAFAHKGPVVV
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEE
DFCVEEGENVFPMVPPNKGNNEMIMKRWEE
EEEECCCCCEECCCCCCCCCCHHHHHCCCC
>Mature Secondary Structure
MKQYTTYEKLQCEEVTGAGHVIQCLKKLGVTTVFGYPGGAILPVYDALYESGLKHILTRH
CCCCCCHHHHHHHHCCCCHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
EQAAIHAAEGYARASGKVGVVFATSGPGATNLVTGLADAYMDSIPLVVITGQVATPLIGK
HHHHHHHHCCHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCC
DGFQEADVVGITVPVTKHNYQVRDVNQLSRIVQEAFYIAESGRPGPVLIDIPKDVQIEKV
CCCCCCCEEEEEEEEECCCEEECCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHH
TSFYNEVIEIPGYKLEPRPDSMKLREVAKAISEAKRPLLYIGGGIIHADGSEELIQFARK
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCEEEECCCHHHHHHHHHH
KGIPVVSTLMGLGAYPPGDPLFLGMLGMHGTYAANMAVTECDLLLALGVRFDDRVTGKLE
CCCHHHHHHHHCCCCCCCCCEEEEHHCCCCCHHCCEEHHHHHHHHHHCCCCCCCCCCEEE
LFSPHSKKVHIDIDPSEFQKNVAVEYPVVGDVKKALHMLLHMSIHTQTDEWLQKVKTWKE
EECCCCCEEEEECCHHHHHCCCEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
EYQLSYKQKESELKPQHVINLVSELTNGEAIVTTEVGQHQMWAAHFYKAKNPRTFLTSGG
HHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCEEEECCC
LGTMGFGFPAAIGAQLAKEEELVICIAGDASFQMNIQELQTIAENNIPVKVFIINNKFLG
CCCCCCCCHHHHHHHHCCCCCEEEEEECCCCEEEEHHHHHHHHCCCCCEEEEEECCHHHH
MVRQWQEMFYENRLSESKIGSPDFVKVAEAYGVKGLRATNATEAKQVMLEAFAHKGPVVV
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEE
DFCVEEGENVFPMVPPNKGNNEMIMKRWEE
EEEECCCCCEECCCCCCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377; 1577690; 9298659 [H]