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The map label for this gene is cspB [H]

Identifier: 49184527

GI number: 49184527

Start: 1544406

End: 1544603

Strand: Reverse

Name: cspB [H]

Synonym: BAS1512

Alternate gene names: 49184527

Gene position: 1544603-1544406 (Counterclockwise)

Preceding gene: 49184536

Following gene: 49184526

Centisome position: 29.54

GC content: 39.39

Gene sequence:

>198_bases
ATGCAAGGAAAAGTAAAATGGTTTAACAACGAAAAAGGTTTTGGATTTATCGAAATGGAAGGCGCTGACGATGTATTCGT
ACATTTCTCTGCGATTCAAGGCGAAGGCTACAAAGCTTTAGAAGAAGGTCAAGAAGTATCTTTCGATATCACTGAAGGAA
ACCGCGGACCTCAAGCTGCTAACGTAGTAAAACTTTAA

Upstream 100 bases:

>100_bases
TTAGCAGAATATTCAAGATTATAGCATTTTTAGTTTATATTATCTTTTAAATGAATTTCACTAAATGAAAAAAGCAAATG
TTTTTAGGAGGAATTGTAAC

Downstream 100 bases:

>100_bases
TCCAACATATAATAGGAGGTTACCTTACTTGGTAACCTCCTTTGTTATTCCTTTGTATATTTCCGTTTTACAGAGCAAAA
ACTATGTGAAATGCGCAATA

Product: cold shock protein CspB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 65; Mature: 65

Protein sequence:

>65_residues
MQGKVKWFNNEKGFGFIEMEGADDVFVHFSAIQGEGYKALEEGQEVSFDITEGNRGPQAANVVKL

Sequences:

>Translated_65_residues
MQGKVKWFNNEKGFGFIEMEGADDVFVHFSAIQGEGYKALEEGQEVSFDITEGNRGPQAANVVKL
>Mature_65_residues
MQGKVKWFNNEKGFGFIEMEGADDVFVHFSAIQGEGYKALEEGQEVSFDITEGNRGPQAANVVKL

Specific function: Binds To And Stimulates The Transcription Of The CCAAT- Containing, Cold-Shock-Inducible Promoters Of The H-Ns And Gyra Proteins. Binds Also To The Inverted Repeat 5'-ATTGG-3'. [C]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1789979, Length=66, Percent_Identity=63.6363636363636, Blast_Score=86, Evalue=4e-19,
Organism=Escherichia coli, GI1786841, Length=66, Percent_Identity=63.6363636363636, Blast_Score=86, Evalue=4e-19,
Organism=Escherichia coli, GI1787839, Length=64, Percent_Identity=60.9375, Blast_Score=83, Evalue=4e-18,
Organism=Escherichia coli, GI1788126, Length=66, Percent_Identity=59.0909090909091, Blast_Score=82, Evalue=6e-18,
Organism=Escherichia coli, GI2367114, Length=66, Percent_Identity=59.0909090909091, Blast_Score=80, Evalue=3e-17,
Organism=Escherichia coli, GI1787834, Length=66, Percent_Identity=60.6060606060606, Blast_Score=77, Evalue=2e-16,
Organism=Escherichia coli, GI1787107, Length=65, Percent_Identity=50.7692307692308, Blast_Score=72, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 7196; Mature: 7196

Theoretical pI: Translated: 4.32; Mature: 4.32

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQGKVKWFNNEKGFGFIEMEGADDVFVHFSAIQGEGYKALEEGQEVSFDITEGNRGPQAA
CCCCEEEECCCCCEEEEEECCCCEEEEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCC
NVVKL
EEEEC
>Mature Secondary Structure
MQGKVKWFNNEKGFGFIEMEGADDVFVHFSAIQGEGYKALEEGQEVSFDITEGNRGPQAA
CCCCEEEECCCCCEEEEEECCCCEEEEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCC
NVVKL
EEEEC

PDB accession: NA

Resolution: NA

Structure class: Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8631682 [H]