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The map label for this gene is napA [H]

Identifier: 49184536

GI number: 49184536

Start: 1551467

End: 1552630

Strand: Reverse

Name: napA [H]

Synonym: BAS1521

Alternate gene names: 49184536

Gene position: 1552630-1551467 (Counterclockwise)

Preceding gene: 49184540

Following gene: 49184527

Centisome position: 29.69

GC content: 37.8

Gene sequence:

>1164_bases
ATGGAATTCGAATTTTTCTTTCAAATTGCACTTATTTTATTAAGTACAAAGCTTGCTGGTGATCTTAGTGTTAGATTAGG
CCAGCCTTCTGTACTCGGTAAATTAATTGTCGGTATCGTTATCGGTCCAGCTGTATTAGGTTGGATTGAAAATTCAGAAC
TTCTAACACAATTAAGTAATGTTGGGGTTATTCTTTTAATGTTTATGGCCGGTCTTGAAACAGACTTAGAGGAATTAAAC
GCAAATCGTAATTCTTCTTTAGCTGTTGCACTTGGAGGTATCATTCTTCCATTCGTAGGTGGTTACGTTTCTGGTCTTGT
TATGGGAATGGAACAAGGAAATGCTGTATTTTTAGGATTACTTCTATGTGCGACAAGTGTTAGTATTTCCGTACAAACAC
TTCGCGATTTAGGAAAGATGAAAACACGTGAAAGTACAACTATGCTGGGCGCAGCTGTATTTGATGACATTCTTGTCGTT
ATTTTATTAGCATTTGCAATGAGCTTCTTAGGTACAGACGATGTTAACTTAACAATGGTTATCTTGAAGAAAGTTGTATT
CTTCGCTTCTATTATTTTAATCGGATGGAAAGGTGTTCCTGCGATTATGCGCTGGTTATCACCACTACGCGTATCTGAGT
CTATCGTGAGCGCGGCTCTTATCATCTGTTTCTCATTCGCATATTTCGGCGAACTATTAGGAATTGCTGGTATTATCGGT
GCTTTCGCAGCTGGTATCGCGATTTCTCAAACGAACTACAAACATGAAGTAGAAAAGAAAGTAGAACCAATCGCTTACGC
TATGTTCGTTCCAGTGTTCTTCGTAAGTATCGGTATGAATATTACATTTGATGGTATCGGCGATCAAATTGGGTTCATCT
TGGCATTAACTGTTATCGCTGTCTTAACGAAACTAATTGGTTGTGGTGTTGGTGCACGAATGACTGGATTTGATGCTAAG
TCTTCTGCTATTATCGGTTCTGGTATGGTTTCTCGTGGTGAAGTTGCACTTATCATTGCAGGAACAGGACTTTCTTCAGG
ACTACTAGCACAAGATTACTTTACAGCAATTGTTATTGTCGTTATTTTAACTACAATGATTACACCACCAATGTTAAAAT
ACACTTTTGGTGCAAAAGATAAAGCAATGAAAGCAAGTAAATAA

Upstream 100 bases:

>100_bases
CTTGTAATCAGACCCTTTGTTTAATGGGTTTTACATATTTTCATTAGGCAAATTCCGAGAAATCGTATGATAAAAATAAA
AATGAAGAGGAGAGGATCCG

Downstream 100 bases:

>100_bases
TTTTAATAAAAGGAGATTGCTATCTTATAAGATAGCAATCTCCTTTTTTGTTTTATCTTTAAACAATCCTGTTCTTTTTT
AACCATTTTTCAAAAATAAT

Product: germination protein gerN

Products: Proton [Cytoplasm]; K (I) [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MEFEFFFQIALILLSTKLAGDLSVRLGQPSVLGKLIVGIVIGPAVLGWIENSELLTQLSNVGVILLMFMAGLETDLEELN
ANRNSSLAVALGGIILPFVGGYVSGLVMGMEQGNAVFLGLLLCATSVSISVQTLRDLGKMKTRESTTMLGAAVFDDILVV
ILLAFAMSFLGTDDVNLTMVILKKVVFFASIILIGWKGVPAIMRWLSPLRVSESIVSAALIICFSFAYFGELLGIAGIIG
AFAAGIAISQTNYKHEVEKKVEPIAYAMFVPVFFVSIGMNITFDGIGDQIGFILALTVIAVLTKLIGCGVGARMTGFDAK
SSAIIGSGMVSRGEVALIIAGTGLSSGLLAQDYFTAIVIVVILTTMITPPMLKYTFGAKDKAMKASK

Sequences:

>Translated_387_residues
MEFEFFFQIALILLSTKLAGDLSVRLGQPSVLGKLIVGIVIGPAVLGWIENSELLTQLSNVGVILLMFMAGLETDLEELN
ANRNSSLAVALGGIILPFVGGYVSGLVMGMEQGNAVFLGLLLCATSVSISVQTLRDLGKMKTRESTTMLGAAVFDDILVV
ILLAFAMSFLGTDDVNLTMVILKKVVFFASIILIGWKGVPAIMRWLSPLRVSESIVSAALIICFSFAYFGELLGIAGIIG
AFAAGIAISQTNYKHEVEKKVEPIAYAMFVPVFFVSIGMNITFDGIGDQIGFILALTVIAVLTKLIGCGVGARMTGFDAK
SSAIIGSGMVSRGEVALIIAGTGLSSGLLAQDYFTAIVIVVILTTMITPPMLKYTFGAKDKAMKASK
>Mature_387_residues
MEFEFFFQIALILLSTKLAGDLSVRLGQPSVLGKLIVGIVIGPAVLGWIENSELLTQLSNVGVILLMFMAGLETDLEELN
ANRNSSLAVALGGIILPFVGGYVSGLVMGMEQGNAVFLGLLLCATSVSISVQTLRDLGKMKTRESTTMLGAAVFDDILVV
ILLAFAMSFLGTDDVNLTMVILKKVVFFASIILIGWKGVPAIMRWLSPLRVSESIVSAALIICFSFAYFGELLGIAGIIG
AFAAGIAISQTNYKHEVEKKVEPIAYAMFVPVFFVSIGMNITFDGIGDQIGFILALTVIAVLTKLIGCGVGARMTGFDAK
SSAIIGSGMVSRGEVALIIAGTGLSSGLLAQDYFTAIVIVVILTTMITPPMLKYTFGAKDKAMKASK

Specific function: This is a Na(+)/H(+) antiporter. It can mediate sodium uptake when a transmembrane pH gradient is applied [H]

COG id: COG0475

COG function: function code P; Kef-type K+ transport systems, membrane components

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the monovalent cation:proton antiporter 2 (CPA2) transporter (TC 2.A.37) family [H]

Homologues:

Organism=Escherichia coli, GI1789749, Length=372, Percent_Identity=26.6129032258064, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1786232, Length=383, Percent_Identity=25.3263707571802, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1786685, Length=363, Percent_Identity=25.8953168044077, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6322367, Length=418, Percent_Identity=25.5980861244019, Blast_Score=128, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006153
- InterPro:   IPR004771 [H]

Pfam domain/function: PF00999 Na_H_Exchanger [H]

EC number: NA

Molecular weight: Translated: 40934; Mature: 40934

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFEFFFQIALILLSTKLAGDLSVRLGQPSVLGKLIVGIVIGPAVLGWIENSELLTQLSN
CCHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
VGVILLMFMAGLETDLEELNANRNSSLAVALGGIILPFVGGYVSGLVMGMEQGNAVFLGL
HHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
LLCATSVSISVQTLRDLGKMKTRESTTMLGAAVFDDILVVILLAFAMSFLGTDDVNLTMV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
ILKKVVFFASIILIGWKGVPAIMRWLSPLRVSESIVSAALIICFSFAYFGELLGIAGIIG
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFAAGIAISQTNYKHEVEKKVEPIAYAMFVPVFFVSIGMNITFDGIGDQIGFILALTVIA
HHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHH
VLTKLIGCGVGARMTGFDAKSSAIIGSGMVSRGEVALIIAGTGLSSGLLAQDYFTAIVIV
HHHHHHHCCCCCCCCCCCCCCCCEECCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHH
VILTTMITPPMLKYTFGAKDKAMKASK
HHHHHHCCCCHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MEFEFFFQIALILLSTKLAGDLSVRLGQPSVLGKLIVGIVIGPAVLGWIENSELLTQLSN
CCHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
VGVILLMFMAGLETDLEELNANRNSSLAVALGGIILPFVGGYVSGLVMGMEQGNAVFLGL
HHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
LLCATSVSISVQTLRDLGKMKTRESTTMLGAAVFDDILVVILLAFAMSFLGTDDVNLTMV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
ILKKVVFFASIILIGWKGVPAIMRWLSPLRVSESIVSAALIICFSFAYFGELLGIAGIIG
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFAAGIAISQTNYKHEVEKKVEPIAYAMFVPVFFVSIGMNITFDGIGDQIGFILALTVIA
HHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHH
VLTKLIGCGVGARMTGFDAKSSAIIGSGMVSRGEVALIIAGTGLSSGLLAQDYFTAIVIV
HHHHHHHCCCCCCCCCCCCCCCCEECCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHH
VILTTMITPPMLKYTFGAKDKAMKASK
HHHHHHCCCCHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; K (I) [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + K (I) [Cytoplasm] = Proton [Cytoplasm] + K (I) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1312090; 1325937 [H]