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The map label for this gene is pflA [H]

Identifier: 49183510

GI number: 49183510

Start: 505963

End: 506694

Strand: Direct

Name: pflA [H]

Synonym: BAS0482

Alternate gene names: 49183510

Gene position: 505963-506694 (Clockwise)

Preceding gene: 49183509

Following gene: 49183511

Centisome position: 9.68

GC content: 33.88

Gene sequence:

>732_bases
ATGGTAAAAGGAAGAATTCATTCTGTAGAGTCTTGTGGTACTGTTGATGGCCCAGGAATTCGTTATGTCATATTTACACA
AGGGTGTTTATTACGTTGTCAATATTGTCATAATGCTGATACGTGGGAGATCGGTAAAGGAAAAGAAATAACAGTCGAAG
AAGTAATGCAGGATGTGACATGTTACCTTCCTTTTATTGAAGCTTCCGGAGGCGGTATAACAGTTAGTGGTGGGGAGCCA
TTATTACAGCTAGATTTCTTAATTGAATTATTTAAGAAATGTAAGGAAATTGGAATTCATACAACAATTGATTCTTCGGG
TGGGTGTTATTCTGAAGAAACAGAATTCCAAAATAAGCTAGACATTTTAATGGAGTACACAGATTTAGTTTTATTGGATT
TGAAACATATTGATTCAAAGAAACATCGTAAATTAACAGGAAAACCAAATGAACATATTTTACAATTTGCTCGTTATTTA
TCGGATAAAAATAAACCGATTTGGGTACGACATGTATTAGTTCCTGGTGTTACTGATAATGAAGAGGATCTACAAAAGTT
ATCTAGCTTTATTCAAAGTCTGTCTAATGTTCAAAAAATTGAAGTGTTACCATATCATAAGCTTGGTGTATATAAATGGG
AGGCACTTGGACATAAGTATCCACTTGCGAATGTAGAACCACCTACTGAAAAAAATGTAGAACAAGCAAGACATATTTTA
CAAGCAGTCTAA

Upstream 100 bases:

>100_bases
AATTAACCGTACAATGCATGAAAGCATGTAAATGAAAAAAGTGTCCCTCTGCATTTTATAAACAGCAGAGGGAGGCTGTT
TCAATAAAGGAGGAAGTAAC

Downstream 100 bases:

>100_bases
TCCAAATATTGGATTAGACTTTTTGCTTTCTTTACAATAAAACCTAGAGTATATATAGAGAAAAGAGTATACTAAAAATA
GAATATCCATATTTTTGTAA

Product: pyruvate formate-lyase-activating enzyme

Products: NA

Alternate protein names: PFL-activating enzyme [H]

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MVKGRIHSVESCGTVDGPGIRYVIFTQGCLLRCQYCHNADTWEIGKGKEITVEEVMQDVTCYLPFIEASGGGITVSGGEP
LLQLDFLIELFKKCKEIGIHTTIDSSGGCYSEETEFQNKLDILMEYTDLVLLDLKHIDSKKHRKLTGKPNEHILQFARYL
SDKNKPIWVRHVLVPGVTDNEEDLQKLSSFIQSLSNVQKIEVLPYHKLGVYKWEALGHKYPLANVEPPTEKNVEQARHIL
QAV

Sequences:

>Translated_243_residues
MVKGRIHSVESCGTVDGPGIRYVIFTQGCLLRCQYCHNADTWEIGKGKEITVEEVMQDVTCYLPFIEASGGGITVSGGEP
LLQLDFLIELFKKCKEIGIHTTIDSSGGCYSEETEFQNKLDILMEYTDLVLLDLKHIDSKKHRKLTGKPNEHILQFARYL
SDKNKPIWVRHVLVPGVTDNEEDLQKLSSFIQSLSNVQKIEVLPYHKLGVYKWEALGHKYPLANVEPPTEKNVEQARHIL
QAV
>Mature_243_residues
MVKGRIHSVESCGTVDGPGIRYVIFTQGCLLRCQYCHNADTWEIGKGKEITVEEVMQDVTCYLPFIEASGGGITVSGGEP
LLQLDFLIELFKKCKEIGIHTTIDSSGGCYSEETEFQNKLDILMEYTDLVLLDLKHIDSKKHRKLTGKPNEHILQFARYL
SDKNKPIWVRHVLVPGVTDNEEDLQKLSSFIQSLSNVQKIEVLPYHKLGVYKWEALGHKYPLANVEPPTEKNVEQARHIL
QAV

Specific function: Activation of pyruvate formate-lyase under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the organic radical-activating enzymes family [H]

Homologues:

Organism=Escherichia coli, GI1787130, Length=240, Percent_Identity=49.1666666666667, Blast_Score=263, Evalue=6e-72,
Organism=Escherichia coli, GI1790389, Length=268, Percent_Identity=26.4925373134328, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI1790839, Length=247, Percent_Identity=29.1497975708502, Blast_Score=94, Evalue=8e-21,
Organism=Escherichia coli, GI226510931, Length=273, Percent_Identity=26.3736263736264, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012838
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 27496; Mature: 27496

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKGRIHSVESCGTVDGPGIRYVIFTQGCLLRCQYCHNADTWEIGKGKEITVEEVMQDVT
CCCCCCCCHHCCCCCCCCCEEEEEEECCHHEEEHHHCCCCCEECCCCCEEEHHHHHHHHH
CYLPFIEASGGGITVSGGEPLLQLDFLIELFKKCKEIGIHTTIDSSGGCYSEETEFQNKL
HHHHEEEECCCEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHH
DILMEYTDLVLLDLKHIDSKKHRKLTGKPNEHILQFARYLSDKNKPIWVRHVLVPGVTDN
HHHHHHHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCCCCC
EEDLQKLSSFIQSLSNVQKIEVLPYHKLGVYKWEALGHKYPLANVEPPTEKNVEQARHIL
HHHHHHHHHHHHHHHCCCEEEECCCHHCCCEEHHHCCCCCCCCCCCCCCCCCHHHHHHHH
QAV
HCC
>Mature Secondary Structure
MVKGRIHSVESCGTVDGPGIRYVIFTQGCLLRCQYCHNADTWEIGKGKEITVEEVMQDVT
CCCCCCCCHHCCCCCCCCCEEEEEEECCHHEEEHHHCCCCCEECCCCCEEEHHHHHHHHH
CYLPFIEASGGGITVSGGEPLLQLDFLIELFKKCKEIGIHTTIDSSGGCYSEETEFQNKL
HHHHEEEECCCEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHH
DILMEYTDLVLLDLKHIDSKKHRKLTGKPNEHILQFARYLSDKNKPIWVRHVLVPGVTDN
HHHHHHHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCCCCC
EEDLQKLSSFIQSLSNVQKIEVLPYHKLGVYKWEALGHKYPLANVEPPTEKNVEQARHIL
HHHHHHHHHHHHHHHCCCEEEECCCHHCCCEEHHHCCCCCCCCCCCCCCCCCHHHHHHHH
QAV
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]