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The map label for this gene is pflB [H]

Identifier: 49183509

GI number: 49183509

Start: 503644

End: 505893

Strand: Direct

Name: pflB [H]

Synonym: BAS0481

Alternate gene names: 49183509

Gene position: 503644-505893 (Clockwise)

Preceding gene: 49183508

Following gene: 49183510

Centisome position: 9.63

GC content: 36.09

Gene sequence:

>2250_bases
ATGACTCAAGTATTAGAAAATGTTAAAAACGCATGGGAAAACTTTAAAGGTGAAAAATGGAAAGCAGAGATTGATGTTCG
CGATTTCATTTTAAATAATGTAAACGTTTACGAGGGAGATGAATCTTTCTTAGCGGGAGCAACTGAAGCAACGAAACAAC
TTTGGGATCAAGTAATGGATTTAACAACGAAAGAACGTGAAAACGGTGGCGTTCTTGATATGGATACAAAAATTGTTTCT
TCTATTACATCACATGAACCAGGATATTTAAATAAAGAGACTGAAAAAGTAGTCGGTTTCCAAACTGATAAACCATTTAA
ACGTTCTTTACAGCCATATGGTGGTATTCGTATGGCGGAACAAGCTTGTGAATCTTATGGATACGAAATGGATAAAGAAC
TTAGTCGTATTTTTAGAGATTGGAGAAAAACTCATAACCAAGGTGTATTTGATGCTTATACACCAGAAATGAGAGCTGCT
CGTAAATCAGGTGTTATTACTGGTCTTCCAGATGCATACGGACGTGGACGTATTATCGGTGACTACCGCCGCGTAGCATT
ATATGGAGTAGATCATTTAATTGAAGCGAAGAAAGCAGATTTAAATTTAACTGGCGGTGTAATGAGTGAAGATACAATGC
GTTTACGCGAAGAATTATCTGAGCAAATACGTGCACTTCAAGAGTTAAAACAAATGGCTGCTTCTCATGGATTTGATATT
TCTAAGCCAGCAACAAATGCTCAAGAAGCATTCCAATGGTTATACTTTGCATATCTTGCAGCAATTAAAGAGCAAAACGG
AGCTGCAATGAGTCTTGGACGTACTTCTACATTCTTAGATATTTATATTGAAAGAGATTTAGCAAATGGTACTTTAACAG
AAGAAGACGTACAAGAAATTGTGGATCACTTCATTATGAAATTACGTCTTGTGAAATTTGCCAGAACACCTGATTATAAT
GAGTTATTCTCTGGTGACCCAACTTGGGTAACTGAGTCTATCGGTGGTATGGCATTAGATGGTCGTCCATTAGTAACAAA
GAACTCATTCCGTTTCTTGCATACATTAGATAATTTAGGACCAGCTCCAGAACCAAACTTAACAGTTCTTTGGTCTAAAC
AATTACCACAGAACTTTAAAAACTACTGTGCGAAAATGTCTATTAAAACATCAGCAATTCAATATGAAAATGATGACATT
ATGCGTGCTGACTACGGCGATGACTACGGAATTGCTTGTTGTGTATCTGCAATGAGAATCGGTAAACAAATGCAATTCTT
TGGCGCACGTGCAAACTTAGCAAAAGCATTGCTATATGCGATTAACGGTGGTAAAGATGAAAAATCTAAAGCGCAAGTTG
GTCCTGAGTATGCACCGATTACTTCTGAAGTATTAGATTATGAAGAAGTTATGCATAAGTTTGATATGACAATGGAATGG
TTAGCGGGTCTATATTTAAACACATTAAATGTTATTCACTACATGCACGATAAATATAGCTACGAACGTATTGAAATGGC
ACTTCATGATACAAATGTTCTTCGTACAATGGCAACAGGTATCGCGGGTCTATCTGTAGTAGCAGATTCTTTAAGTGCAA
TTAAATATGCAAAAGTAAAACCAATTCGCGATGAAAACGGAATCGCAGTTGACTTTGAAATTGAAGGAGATTTCCCTAAA
TACGGTAACAATGATGACCGTGTAGATGAAATTGCTGTAAATCTTGTGAAAACATTTATGAATAAACTTCGTAAACATAA
AACATACAGAAATTCTGTTCATACAATGTCAATCTTAACAATCACATCTAACGTTGTATACGGTAAGAAAACTGGTAACA
CTCCAGATGGTCGCCGTACTGGAGAACCATTTGCACCAGGTGCGAACCCAATGCATGGACGTGATACAAAAGGTGCGCTA
GCTTCATTATTATCTGTAGCTAAATTACCATATGAAGATGCACAAGATGGTATTTCTAATACATTCTCTATTATTCCAAA
AGCACTTGGTAAAGAGGATGAAGTACAAGTACGTAACTTAGTATCAATGCTTGATGGTTATGCAGTAAAAGAAGGACATC
ACTTAAATATTAACGTATTTAACCGTGAAACATTAATGGATGCAATGGAACATCCTGAGAAATATCCACAATTAACAATT
CGCGTATCTGGTTACGCTGTAAACTTTATTAAATTAACTCGTGAACAACAAATCGATGTAATTAACCGTACAATGCATGA
AAGCATGTAA

Upstream 100 bases:

>100_bases
ACTATTTCGTTCACTTTCGTAAAAAAGTAGTATTCTAAGTGTGTAAGCTGTTATATAAAAAATCTTAGTCCTGTACTAAT
CAAAAATGGAGGAGATTAGG

Downstream 100 bases:

>100_bases
ATGAAAAAAGTGTCCCTCTGCATTTTATAAACAGCAGAGGGAGGCTGTTTCAATAAAGGAGGAAGTAACATGGTAAAAGG
AAGAATTCATTCTGTAGAGT

Product: formate acetyltransferase

Products: NA

Alternate protein names: Pyruvate formate-lyase [H]

Number of amino acids: Translated: 749; Mature: 748

Protein sequence:

>749_residues
MTQVLENVKNAWENFKGEKWKAEIDVRDFILNNVNVYEGDESFLAGATEATKQLWDQVMDLTTKERENGGVLDMDTKIVS
SITSHEPGYLNKETEKVVGFQTDKPFKRSLQPYGGIRMAEQACESYGYEMDKELSRIFRDWRKTHNQGVFDAYTPEMRAA
RKSGVITGLPDAYGRGRIIGDYRRVALYGVDHLIEAKKADLNLTGGVMSEDTMRLREELSEQIRALQELKQMAASHGFDI
SKPATNAQEAFQWLYFAYLAAIKEQNGAAMSLGRTSTFLDIYIERDLANGTLTEEDVQEIVDHFIMKLRLVKFARTPDYN
ELFSGDPTWVTESIGGMALDGRPLVTKNSFRFLHTLDNLGPAPEPNLTVLWSKQLPQNFKNYCAKMSIKTSAIQYENDDI
MRADYGDDYGIACCVSAMRIGKQMQFFGARANLAKALLYAINGGKDEKSKAQVGPEYAPITSEVLDYEEVMHKFDMTMEW
LAGLYLNTLNVIHYMHDKYSYERIEMALHDTNVLRTMATGIAGLSVVADSLSAIKYAKVKPIRDENGIAVDFEIEGDFPK
YGNNDDRVDEIAVNLVKTFMNKLRKHKTYRNSVHTMSILTITSNVVYGKKTGNTPDGRRTGEPFAPGANPMHGRDTKGAL
ASLLSVAKLPYEDAQDGISNTFSIIPKALGKEDEVQVRNLVSMLDGYAVKEGHHLNINVFNRETLMDAMEHPEKYPQLTI
RVSGYAVNFIKLTREQQIDVINRTMHESM

Sequences:

>Translated_749_residues
MTQVLENVKNAWENFKGEKWKAEIDVRDFILNNVNVYEGDESFLAGATEATKQLWDQVMDLTTKERENGGVLDMDTKIVS
SITSHEPGYLNKETEKVVGFQTDKPFKRSLQPYGGIRMAEQACESYGYEMDKELSRIFRDWRKTHNQGVFDAYTPEMRAA
RKSGVITGLPDAYGRGRIIGDYRRVALYGVDHLIEAKKADLNLTGGVMSEDTMRLREELSEQIRALQELKQMAASHGFDI
SKPATNAQEAFQWLYFAYLAAIKEQNGAAMSLGRTSTFLDIYIERDLANGTLTEEDVQEIVDHFIMKLRLVKFARTPDYN
ELFSGDPTWVTESIGGMALDGRPLVTKNSFRFLHTLDNLGPAPEPNLTVLWSKQLPQNFKNYCAKMSIKTSAIQYENDDI
MRADYGDDYGIACCVSAMRIGKQMQFFGARANLAKALLYAINGGKDEKSKAQVGPEYAPITSEVLDYEEVMHKFDMTMEW
LAGLYLNTLNVIHYMHDKYSYERIEMALHDTNVLRTMATGIAGLSVVADSLSAIKYAKVKPIRDENGIAVDFEIEGDFPK
YGNNDDRVDEIAVNLVKTFMNKLRKHKTYRNSVHTMSILTITSNVVYGKKTGNTPDGRRTGEPFAPGANPMHGRDTKGAL
ASLLSVAKLPYEDAQDGISNTFSIIPKALGKEDEVQVRNLVSMLDGYAVKEGHHLNINVFNRETLMDAMEHPEKYPQLTI
RVSGYAVNFIKLTREQQIDVINRTMHESM
>Mature_748_residues
TQVLENVKNAWENFKGEKWKAEIDVRDFILNNVNVYEGDESFLAGATEATKQLWDQVMDLTTKERENGGVLDMDTKIVSS
ITSHEPGYLNKETEKVVGFQTDKPFKRSLQPYGGIRMAEQACESYGYEMDKELSRIFRDWRKTHNQGVFDAYTPEMRAAR
KSGVITGLPDAYGRGRIIGDYRRVALYGVDHLIEAKKADLNLTGGVMSEDTMRLREELSEQIRALQELKQMAASHGFDIS
KPATNAQEAFQWLYFAYLAAIKEQNGAAMSLGRTSTFLDIYIERDLANGTLTEEDVQEIVDHFIMKLRLVKFARTPDYNE
LFSGDPTWVTESIGGMALDGRPLVTKNSFRFLHTLDNLGPAPEPNLTVLWSKQLPQNFKNYCAKMSIKTSAIQYENDDIM
RADYGDDYGIACCVSAMRIGKQMQFFGARANLAKALLYAINGGKDEKSKAQVGPEYAPITSEVLDYEEVMHKFDMTMEWL
AGLYLNTLNVIHYMHDKYSYERIEMALHDTNVLRTMATGIAGLSVVADSLSAIKYAKVKPIRDENGIAVDFEIEGDFPKY
GNNDDRVDEIAVNLVKTFMNKLRKHKTYRNSVHTMSILTITSNVVYGKKTGNTPDGRRTGEPFAPGANPMHGRDTKGALA
SLLSVAKLPYEDAQDGISNTFSIIPKALGKEDEVQVRNLVSMLDGYAVKEGHHLNINVFNRETLMDAMEHPEKYPQLTIR
VSGYAVNFIKLTREQQIDVINRTMHESM

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1787131, Length=760, Percent_Identity=65.2631578947368, Blast_Score=1056, Evalue=0.0,
Organism=Escherichia coli, GI48994926, Length=751, Percent_Identity=65.7789613848202, Blast_Score=1030, Evalue=0.0,
Organism=Escherichia coli, GI1787044, Length=574, Percent_Identity=27.1777003484321, Blast_Score=186, Evalue=6e-48,
Organism=Escherichia coli, GI1790388, Length=698, Percent_Identity=25.3581661891117, Blast_Score=156, Evalue=4e-39,
Organism=Escherichia coli, GI1788933, Length=61, Percent_Identity=70.4918032786885, Blast_Score=90, Evalue=5e-19,

Paralogues:

None

Copy number: 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005949
- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 84539; Mature: 84408

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQVLENVKNAWENFKGEKWKAEIDVRDFILNNVNVYEGDESFLAGATEATKQLWDQVMD
CHHHHHHHHHHHHHCCCCCEEEECCHHHHHHCCCEEEECCHHHHCCHHHHHHHHHHHHHH
LTTKERENGGVLDMDTKIVSSITSHEPGYLNKETEKVVGFQTDKPFKRSLQPYGGIRMAE
HHHHHCCCCCEEEEHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCHHHCCCCCCCHHHHH
QACESYGYEMDKELSRIFRDWRKTHNQGVFDAYTPEMRAARKSGVITGLPDAYGRGRIIG
HHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCEECCCCCCCCCCEEH
DYRRVALYGVDHLIEAKKADLNLTGGVMSEDTMRLREELSEQIRALQELKQMAASHGFDI
HHHHHHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SKPATNAQEAFQWLYFAYLAAIKEQNGAAMSLGRTSTFLDIYIERDLANGTLTEEDVQEI
CCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEEEEEEEECCCCCCCCHHHHHHH
VDHFIMKLRLVKFARTPDYNELFSGDPTWVTESIGGMALDGRPLVTKNSFRFLHTLDNLG
HHHHHHHHHHHHHHCCCCHHHHHCCCCCCHHHCCCCEEECCCCEEECCCHHHHHHHHHCC
PAPEPNLTVLWSKQLPQNFKNYCAKMSIKTSAIQYENDDIMRADYGDDYGIACCVSAMRI
CCCCCCEEEEECHHCHHHHHHHHHHCCEEHHEEEECCCCEEECCCCCCCHHHHHHHHHHH
GKQMQFFGARANLAKALLYAINGGKDEKSKAQVGPEYAPITSEVLDYEEVMHKFDMTMEW
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LAGLYLNTLNVIHYMHDKYSYERIEMALHDTNVLRTMATGIAGLSVVADSLSAIKYAKVK
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PIRDENGIAVDFEIEGDFPKYGNNDDRVDEIAVNLVKTFMNKLRKHKTYRNSVHTMSILT
CCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEEE
ITSNVVYGKKTGNTPDGRRTGEPFAPGANPMHGRDTKGALASLLSVAKLPYEDAQDGISN
EECCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHH
TFSIIPKALGKEDEVQVRNLVSMLDGYAVKEGHHLNINVFNRETLMDAMEHPEKYPQLTI
HHHHHHHHHCCCHHHHHHHHHHHHCCCEECCCCEEEEEEECHHHHHHHHHCCCCCCEEEE
RVSGYAVNFIKLTREQQIDVINRTMHESM
EEECEEEEEEEHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TQVLENVKNAWENFKGEKWKAEIDVRDFILNNVNVYEGDESFLAGATEATKQLWDQVMD
HHHHHHHHHHHHHCCCCCEEEECCHHHHHHCCCEEEECCHHHHCCHHHHHHHHHHHHHH
LTTKERENGGVLDMDTKIVSSITSHEPGYLNKETEKVVGFQTDKPFKRSLQPYGGIRMAE
HHHHHCCCCCEEEEHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCHHHCCCCCCCHHHHH
QACESYGYEMDKELSRIFRDWRKTHNQGVFDAYTPEMRAARKSGVITGLPDAYGRGRIIG
HHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCEECCCCCCCCCCEEH
DYRRVALYGVDHLIEAKKADLNLTGGVMSEDTMRLREELSEQIRALQELKQMAASHGFDI
HHHHHHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SKPATNAQEAFQWLYFAYLAAIKEQNGAAMSLGRTSTFLDIYIERDLANGTLTEEDVQEI
CCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEEEEEEEECCCCCCCCHHHHHHH
VDHFIMKLRLVKFARTPDYNELFSGDPTWVTESIGGMALDGRPLVTKNSFRFLHTLDNLG
HHHHHHHHHHHHHHCCCCHHHHHCCCCCCHHHCCCCEEECCCCEEECCCHHHHHHHHHCC
PAPEPNLTVLWSKQLPQNFKNYCAKMSIKTSAIQYENDDIMRADYGDDYGIACCVSAMRI
CCCCCCEEEEECHHCHHHHHHHHHHCCEEHHEEEECCCCEEECCCCCCCHHHHHHHHHHH
GKQMQFFGARANLAKALLYAINGGKDEKSKAQVGPEYAPITSEVLDYEEVMHKFDMTMEW
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LAGLYLNTLNVIHYMHDKYSYERIEMALHDTNVLRTMATGIAGLSVVADSLSAIKYAKVK
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PIRDENGIAVDFEIEGDFPKYGNNDDRVDEIAVNLVKTFMNKLRKHKTYRNSVHTMSILT
CCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEEE
ITSNVVYGKKTGNTPDGRRTGEPFAPGANPMHGRDTKGALASLLSVAKLPYEDAQDGISN
EECCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHH
TFSIIPKALGKEDEVQVRNLVSMLDGYAVKEGHHLNINVFNRETLMDAMEHPEKYPQLTI
HHHHHHHHHCCCHHHHHHHHHHHHCCCEECCCCEEEEEEECHHHHHHHHHCCCCCCEEEE
RVSGYAVNFIKLTREQQIDVINRTMHESM
EEECEEEEEEEHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA