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The map label for this gene is yfkE [H]

Identifier: 49183434

GI number: 49183434

Start: 438919

End: 439983

Strand: Direct

Name: yfkE [H]

Synonym: BAS0406

Alternate gene names: 49183434

Gene position: 438919-439983 (Clockwise)

Preceding gene: 49183431

Following gene: 49183435

Centisome position: 8.39

GC content: 36.9

Gene sequence:

>1065_bases
ATGGAAAGCATGTTTAATAAAATATTTCTTATCGTGGCGCTTATAGGTGTACCACTGTCTGTACTTGGGAAAACACTTCA
TTGGCCTCAAACAATTATGTTTGCTGTGTATTGTATTACGATTATCGCATTAGCGGGTTTTATGGGGAGAGCGACAGAAA
GTTTGGCAATTGTATCTGGTCCTAGAATAGGTGGATTATTAAATGCTACTTTCGGTAATGCTGTTGAACTAATCATTTCA
ATTTTTGCACTTCAGGCAGGATTAATTGAGGTGGTATTAGCTTCTTTAACGGGTTCTGTACTTGGAAATTTATTATTAGT
AGGAGGGCTATCCTTCTTTATAGGAGGGCTTAAATACAAAAGACAAAGTTTTAATGTGTATGATGCAAGGCATAATTCAG
CTTTATTAATCTTTGCTGTAGTAGTAGCATTCGTTATTCCAGAGATTTTTTCAATGAAGATGGACGCGGGAAAGACGTAT
CAATTAAGTATCGGTGTCTCGATTATTATGATTATTATGTACCTTGCTGCATTGTTATTTAAGTTAGTTACGCACCGCGG
TGTATATCAACATAAAAGTGATGAAGTAGCTCATGAAGAAGAACCAGAGTGGTCGAAAGGCAAAGCGCTACTCATTTTAG
CGATAGCGACAATTGCGGTAGCTTATGTATCAGAGGCGCTTGTGCATACATTTGAGACGGTCGCAAAATCATTTGGTTGG
TCAGAGTTATTTATAGGGGTTATTATCGTTGCGATTGTCGGAAATGCGGCGGAACATGCATCTGCAATTATTATGGCTTA
TAAAAATAAAGTAAATATTGCAGTAGAAATTGCAGTAGGTTCTACATTGCAAATTGCTATGTTTGTTGCGCCTGTATTAG
TACTGCTTTCTATGTTCTTCGCGCAAAGGATGCCTTTAGTATTTACAATACCAGAACTTGTTTCTATGATTACAGCTGTT
TTCTTAACGATTGCGATTTCAAATGATGGAGATACAAACTGGTTTGAAGGCGGCACTTTATTAGCAGCGTATGTCATTAT
GGGAATTGGTTTTTATTTATTATGA

Upstream 100 bases:

>100_bases
GTTTTCTTCAATTCAATTTTTTAAACGTAATTGTGATGAAATCGGACAAGTTAGGAGAAGAATAATAATAATATCGAGGA
GAAATACATACGATGGAAGG

Downstream 100 bases:

>100_bases
AAAATAGGCATAACAATTGTGCCCAGAGAAAACAATAAACGAAGTAGTATATATACCTTTACGTAGGAAGGAGCCAGACA
GGTGAAACGAGTATACAGCA

Product: calcium/proton exchanger

Products: Na (I) [Periplasm]; Ca (II) [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MESMFNKIFLIVALIGVPLSVLGKTLHWPQTIMFAVYCITIIALAGFMGRATESLAIVSGPRIGGLLNATFGNAVELIIS
IFALQAGLIEVVLASLTGSVLGNLLLVGGLSFFIGGLKYKRQSFNVYDARHNSALLIFAVVVAFVIPEIFSMKMDAGKTY
QLSIGVSIIMIIMYLAALLFKLVTHRGVYQHKSDEVAHEEEPEWSKGKALLILAIATIAVAYVSEALVHTFETVAKSFGW
SELFIGVIIVAIVGNAAEHASAIIMAYKNKVNIAVEIAVGSTLQIAMFVAPVLVLLSMFFAQRMPLVFTIPELVSMITAV
FLTIAISNDGDTNWFEGGTLLAAYVIMGIGFYLL

Sequences:

>Translated_354_residues
MESMFNKIFLIVALIGVPLSVLGKTLHWPQTIMFAVYCITIIALAGFMGRATESLAIVSGPRIGGLLNATFGNAVELIIS
IFALQAGLIEVVLASLTGSVLGNLLLVGGLSFFIGGLKYKRQSFNVYDARHNSALLIFAVVVAFVIPEIFSMKMDAGKTY
QLSIGVSIIMIIMYLAALLFKLVTHRGVYQHKSDEVAHEEEPEWSKGKALLILAIATIAVAYVSEALVHTFETVAKSFGW
SELFIGVIIVAIVGNAAEHASAIIMAYKNKVNIAVEIAVGSTLQIAMFVAPVLVLLSMFFAQRMPLVFTIPELVSMITAV
FLTIAISNDGDTNWFEGGTLLAAYVIMGIGFYLL
>Mature_354_residues
MESMFNKIFLIVALIGVPLSVLGKTLHWPQTIMFAVYCITIIALAGFMGRATESLAIVSGPRIGGLLNATFGNAVELIIS
IFALQAGLIEVVLASLTGSVLGNLLLVGGLSFFIGGLKYKRQSFNVYDARHNSALLIFAVVVAFVIPEIFSMKMDAGKTY
QLSIGVSIIMIIMYLAALLFKLVTHRGVYQHKSDEVAHEEEPEWSKGKALLILAIATIAVAYVSEALVHTFETVAKSFGW
SELFIGVIIVAIVGNAAEHASAIIMAYKNKVNIAVEIAVGSTLQIAMFVAPVLVLLSMFFAQRMPLVFTIPELVSMITAV
FLTIAISNDGDTNWFEGGTLLAAYVIMGIGFYLL

Specific function: According To Ref.1 The Natural Activity Of This Protein Is As A Calcium/Proton Antiporter, But It Can Act As A Weak Sodium/Proton Antiporter. The Presence Of A Preferred Substrate And Normal Ph Cytoplasmic Values Might Prevent Significant Sodium/Proton An

COG id: COG0387

COG function: function code P; Ca2+/H+ antiporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium/potassium/calcium exchanger family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6320075, Length=354, Percent_Identity=38.135593220339, Blast_Score=204, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6324008, Length=389, Percent_Identity=28.2776349614396, Blast_Score=135, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004713
- InterPro:   IPR004798
- InterPro:   IPR004837 [H]

Pfam domain/function: PF01699 Na_Ca_ex [H]

EC number: NA

Molecular weight: Translated: 38177; Mature: 38177

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESMFNKIFLIVALIGVPLSVLGKTLHWPQTIMFAVYCITIIALAGFMGRATESLAIVSG
CHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECC
PRIGGLLNATFGNAVELIISIFALQAGLIEVVLASLTGSVLGNLLLVGGLSFFIGGLKYK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
RQSFNVYDARHNSALLIFAVVVAFVIPEIFSMKMDAGKTYQLSIGVSIIMIIMYLAALLF
HHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHH
KLVTHRGVYQHKSDEVAHEEEPEWSKGKALLILAIATIAVAYVSEALVHTFETVAKSFGW
HHHHHCCHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
SELFIGVIIVAIVGNAAEHASAIIMAYKNKVNIAVEIAVGSTLQIAMFVAPVLVLLSMFF
HHHHHHHHHHHHHCCCHHHHHHHHHEECCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHH
AQRMPLVFTIPELVSMITAVFLTIAISNDGDTNWFEGGTLLAAYVIMGIGFYLL
HCCCCCEEEHHHHHHHHHHHHHHHEECCCCCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MESMFNKIFLIVALIGVPLSVLGKTLHWPQTIMFAVYCITIIALAGFMGRATESLAIVSG
CHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECC
PRIGGLLNATFGNAVELIISIFALQAGLIEVVLASLTGSVLGNLLLVGGLSFFIGGLKYK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
RQSFNVYDARHNSALLIFAVVVAFVIPEIFSMKMDAGKTYQLSIGVSIIMIIMYLAALLF
HHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHH
KLVTHRGVYQHKSDEVAHEEEPEWSKGKALLILAIATIAVAYVSEALVHTFETVAKSFGW
HHHHHCCHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
SELFIGVIIVAIVGNAAEHASAIIMAYKNKVNIAVEIAVGSTLQIAMFVAPVLVLLSMFF
HHHHHHHHHHHHHCCCHHHHHHHHHEECCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHH
AQRMPLVFTIPELVSMITAVFLTIAISNDGDTNWFEGGTLLAAYVIMGIGFYLL
HCCCCCEEEHHHHHHHHHHHHHHHEECCCCCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Cytoplasm]; Proton [Periplasm]; Ca (II) [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: Na (I) [Cytoplasm] + Proton [Periplasm] = Proton [Cytoplasm] + Na (I) [Periplasm] Ca (II) [Cytoplasm] + Proton [Periplasm] = Ca (II) [Periplasm] + Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]