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The map label for this gene is proS2

Identifier: 49183410

GI number: 49183410

Start: 414397

End: 415923

Strand: Direct

Name: proS2

Synonym: BAS0382

Alternate gene names: 49183410

Gene position: 414397-415923 (Clockwise)

Preceding gene: 49183409

Following gene: 49183412

Centisome position: 7.93

GC content: 35.76

Gene sequence:

>1527_bases
ATGGCAAAAGAACAAGTACAAGCAATTACGAAGATGGAAGAGGACTTTGCACAGTGGTATACAGATATTGTGAAAAAAGC
AGAACTTGTTGATTATTCAAGTGTAAAAGGATGTATGATTCTACGTCCGTACGGTTATGCGTTATGGGAAAATATGCAAA
AAGTTATGGATGAGAAGTTAAAAGCAACTGGTCATGAAAATGTGTATATGCCAATGTTTATCCCAGAGAGCTTATTACAA
AAAGAGAAGGATCATGTAGAAGGATTCGCTCCTGAAGTAGCATGGGTAACACATGGTGGGGATGAAAAGTTAGCGGAAAG
ACTTTGTGTACGCCCTACATCTGAAACTTTATTCTGTGAGCACTTTTCAAAAATTGTGCAATCCTATAATGATTTGCCAA
AGCTATATAATCAGTGGTGTTCAGTAGTTCGTTGGGAGAAAACAACTAGGCCATTCCTTCGTACAACAGAATTCTTATGG
CAAGAGGGACATACAATTCATGAAACAGCAGAAGAATCCCAAGCTGAAACGTTAAATATTTTAAATCTATATGCTTCTTT
CTGTGAAGACTACTTAGCGATACCGGTAATTAAAGGACAAAAAACAGAAAAAGAAAAATTTGCGGGTGCAAAGGCAACTT
ATACGATTGAAAGCTTAATGCATGATGGAAAAGCACTTCAAACAGGAACATCCCATAACTTTGGAACGAATTTCTCTGAA
GCATTTGATATTAAATTTTTAGATCGTAACGGTAAGTGGCAATATGTACACCAAACATCTTGGGGCGTATCAACAAGGAT
GATAGGTGGACTGATTATGGTTCATAGTGATAATAATGGGCTTGTAATGCCTCCTAAAGTCGCACCAGTGCAAGTTGTTA
TTGTACCGATTGCGCAGCATAAAGAAGGCGTTTTAGCGAAAGCAACAGAGTTACAAGGACATATTCAAAAGGTTGCACGA
GTAAAAATAGATGCTAGTAATAAAACACCAGGTTGGAAATTTAATGAGTATGAAATGAAAGGTATTCCAATTCGATTAGA
AGTTGGTCCTAAAGATATTGAAAAGAATCAAGTTGTACTCGTAAGAAGAGATACGAAAGAAAAAGAGTTTATATCAATGG
ATCAATTAGAAGAACGCATTCCAGCACTACTTGAGGAAATTCATAACTCTTTATTTAATAAAGCAAAAGTATTTCGTGAT
GAAAATACGTATAGTGTGACGAATTTTGAAGAGATGAAAAAATTGGCTGATGAAAAGCAAGGGTTTATTAAAGCAATGTG
GTGTGGAGAATTAGCTTGTGAAGAGAAACTAAAAGAAGAAGTTGCTTCTCGCTGTATGCCTTTTGAACAAGAACATTTAG
CAGAAGAATGTGTTTGTTGTGTAAACAAATGGTGTATTGGGGAAAAGCGTATTAATGCTCTTTTGGATAGGAAGGATGAA
AAGGACTTTGGGCTGCATAATAAATGCAATCCAAAGTCCTTTTTAGTTTTACAATTTGTTTCATGTATTTTTAAGATGGA
AGGATGA

Upstream 100 bases:

>100_bases
CAATTTTTGTGCATGAATCAGGGTGGTAACGCCGGCAAAGCTCGGTCCCTATTTTGGGACGCGGGCTTTTTTGTATTTTC
TAGAAGGAGGAAGACTGACT

Downstream 100 bases:

>100_bases
GTTTTTATTTTGCGTTTTTACGGCGAATAAATAGTAACATACCAATTAGGAATGTTGAAAGTCCCATTAAAATAGATGCA
GGATAATGTGTTGCAGTATT

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase 2; ProRS 2

Number of amino acids: Translated: 508; Mature: 507

Protein sequence:

>508_residues
MAKEQVQAITKMEEDFAQWYTDIVKKAELVDYSSVKGCMILRPYGYALWENMQKVMDEKLKATGHENVYMPMFIPESLLQ
KEKDHVEGFAPEVAWVTHGGDEKLAERLCVRPTSETLFCEHFSKIVQSYNDLPKLYNQWCSVVRWEKTTRPFLRTTEFLW
QEGHTIHETAEESQAETLNILNLYASFCEDYLAIPVIKGQKTEKEKFAGAKATYTIESLMHDGKALQTGTSHNFGTNFSE
AFDIKFLDRNGKWQYVHQTSWGVSTRMIGGLIMVHSDNNGLVMPPKVAPVQVVIVPIAQHKEGVLAKATELQGHIQKVAR
VKIDASNKTPGWKFNEYEMKGIPIRLEVGPKDIEKNQVVLVRRDTKEKEFISMDQLEERIPALLEEIHNSLFNKAKVFRD
ENTYSVTNFEEMKKLADEKQGFIKAMWCGELACEEKLKEEVASRCMPFEQEHLAEECVCCVNKWCIGEKRINALLDRKDE
KDFGLHNKCNPKSFLVLQFVSCIFKMEG

Sequences:

>Translated_508_residues
MAKEQVQAITKMEEDFAQWYTDIVKKAELVDYSSVKGCMILRPYGYALWENMQKVMDEKLKATGHENVYMPMFIPESLLQ
KEKDHVEGFAPEVAWVTHGGDEKLAERLCVRPTSETLFCEHFSKIVQSYNDLPKLYNQWCSVVRWEKTTRPFLRTTEFLW
QEGHTIHETAEESQAETLNILNLYASFCEDYLAIPVIKGQKTEKEKFAGAKATYTIESLMHDGKALQTGTSHNFGTNFSE
AFDIKFLDRNGKWQYVHQTSWGVSTRMIGGLIMVHSDNNGLVMPPKVAPVQVVIVPIAQHKEGVLAKATELQGHIQKVAR
VKIDASNKTPGWKFNEYEMKGIPIRLEVGPKDIEKNQVVLVRRDTKEKEFISMDQLEERIPALLEEIHNSLFNKAKVFRD
ENTYSVTNFEEMKKLADEKQGFIKAMWCGELACEEKLKEEVASRCMPFEQEHLAEECVCCVNKWCIGEKRINALLDRKDE
KDFGLHNKCNPKSFLVLQFVSCIFKMEG
>Mature_507_residues
AKEQVQAITKMEEDFAQWYTDIVKKAELVDYSSVKGCMILRPYGYALWENMQKVMDEKLKATGHENVYMPMFIPESLLQK
EKDHVEGFAPEVAWVTHGGDEKLAERLCVRPTSETLFCEHFSKIVQSYNDLPKLYNQWCSVVRWEKTTRPFLRTTEFLWQ
EGHTIHETAEESQAETLNILNLYASFCEDYLAIPVIKGQKTEKEKFAGAKATYTIESLMHDGKALQTGTSHNFGTNFSEA
FDIKFLDRNGKWQYVHQTSWGVSTRMIGGLIMVHSDNNGLVMPPKVAPVQVVIVPIAQHKEGVLAKATELQGHIQKVARV
KIDASNKTPGWKFNEYEMKGIPIRLEVGPKDIEKNQVVLVRRDTKEKEFISMDQLEERIPALLEEIHNSLFNKAKVFRDE
NTYSVTNFEEMKKLADEKQGFIKAMWCGELACEEKLKEEVASRCMPFEQEHLAEECVCCVNKWCIGEKRINALLDRKDEK
DFGLHNKCNPKSFLVLQFVSCIFKMEG

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro)

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily

Homologues:

Organism=Homo sapiens, GI62241042, Length=484, Percent_Identity=45.4545454545455, Blast_Score=423, Evalue=1e-118,
Organism=Escherichia coli, GI1786392, Length=158, Percent_Identity=26.5822784810127, Blast_Score=64, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI71991342, Length=479, Percent_Identity=42.5887265135699, Blast_Score=407, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI71991346, Length=361, Percent_Identity=46.5373961218837, Blast_Score=353, Evalue=8e-98,
Organism=Saccharomyces cerevisiae, GI6321808, Length=487, Percent_Identity=48.2546201232033, Blast_Score=445, Evalue=1e-126,
Organism=Drosophila melanogaster, GI24649468, Length=492, Percent_Identity=43.2926829268293, Blast_Score=411, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24649466, Length=492, Percent_Identity=43.4959349593496, Blast_Score=410, Evalue=1e-115,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP2_BACAN (Q81Z76)

Other databases:

- EMBL:   AE016879
- EMBL:   AE017225
- EMBL:   AE017334
- RefSeq:   NP_842940.1
- RefSeq:   YP_017016.1
- RefSeq:   YP_026662.1
- HSSP:   O26708
- ProteinModelPortal:   Q81Z76
- SMR:   Q81Z76
- IntAct:   Q81Z76
- EnsemblBacteria:   EBBACT00000010128
- EnsemblBacteria:   EBBACT00000015405
- EnsemblBacteria:   EBBACT00000019314
- GeneID:   1086703
- GeneID:   2815379
- GeneID:   2850540
- GenomeReviews:   AE016879_GR
- GenomeReviews:   AE017225_GR
- GenomeReviews:   AE017334_GR
- KEGG:   ban:BA_0396
- KEGG:   bar:GBAA_0396
- KEGG:   bat:BAS0382
- TIGR:   BA_0396
- TIGR:   GBAA_0396
- GeneTree:   EBGT00050000001167
- HOGENOM:   HBG334108
- OMA:   PGWKFNE
- ProtClustDB:   PRK08661
- BioCyc:   BANT260799:BAS0382-MONOMER
- BioCyc:   BANT261594:GBAA0396-MONOMER
- BRENDA:   6.1.1.15
- GO:   GO:0005737
- HAMAP:   MF_01571
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004499
- InterPro:   IPR017449
- InterPro:   IPR016061
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.30.110.30
- PANTHER:   PTHR11451:SF6
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00408

Pfam domain/function: PF03129 HGTP_anticodon; PF09180 ProRS-C_1; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd; SSF64586 Pro-tRNA_synth_II_C

EC number: =6.1.1.15

Molecular weight: Translated: 58479; Mature: 58348

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEQVQAITKMEEDFAQWYTDIVKKAELVDYSSVKGCMILRPYGYALWENMQKVMDEKL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHH
KATGHENVYMPMFIPESLLQKEKDHVEGFAPEVAWVTHGGDEKLAERLCVRPTSETLFCE
HHCCCCCEEEEEECCHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHCCCCCCHHHHH
HFSKIVQSYNDLPKLYNQWCSVVRWEKTTRPFLRTTEFLWQEGHTIHETAEESQAETLNI
HHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
LNLYASFCEDYLAIPVIKGQKTEKEKFAGAKATYTIESLMHDGKALQTGTSHNFGTNFSE
HHHHHHHHHHHHCCEEECCCCCHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
AFDIKFLDRNGKWQYVHQTSWGVSTRMIGGLIMVHSDNNGLVMPPKVAPVQVVIVPIAQH
EEEEEEECCCCCEEEEEECCCCHHHHHHCCEEEEEECCCCEEECCCCCCEEEEEEECCCC
KEGVLAKATELQGHIQKVARVKIDASNKTPGWKFNEYEMKGIPIRLEVGPKDIEKNQVVL
CCCHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCEEECCEEEEEECCCCCCCCCCEEE
VRRDTKEKEFISMDQLEERIPALLEEIHNSLFNKAKVFRDENTYSVTNFEEMKKLADEKQ
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHC
GFIKAMWCGELACEEKLKEEVASRCMPFEQEHLAEECVCCVNKWCIGEKRINALLDRKDE
CCEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCC
KDFGLHNKCNPKSFLVLQFVSCIFKMEG
CCCCCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AKEQVQAITKMEEDFAQWYTDIVKKAELVDYSSVKGCMILRPYGYALWENMQKVMDEKL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHH
KATGHENVYMPMFIPESLLQKEKDHVEGFAPEVAWVTHGGDEKLAERLCVRPTSETLFCE
HHCCCCCEEEEEECCHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHCCCCCCHHHHH
HFSKIVQSYNDLPKLYNQWCSVVRWEKTTRPFLRTTEFLWQEGHTIHETAEESQAETLNI
HHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
LNLYASFCEDYLAIPVIKGQKTEKEKFAGAKATYTIESLMHDGKALQTGTSHNFGTNFSE
HHHHHHHHHHHHCCEEECCCCCHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
AFDIKFLDRNGKWQYVHQTSWGVSTRMIGGLIMVHSDNNGLVMPPKVAPVQVVIVPIAQH
EEEEEEECCCCCEEEEEECCCCHHHHHHCCEEEEEECCCCEEECCCCCCEEEEEEECCCC
KEGVLAKATELQGHIQKVARVKIDASNKTPGWKFNEYEMKGIPIRLEVGPKDIEKNQVVL
CCCHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCEEECCEEEEEECCCCCCCCCCEEE
VRRDTKEKEFISMDQLEERIPALLEEIHNSLFNKAKVFRDENTYSVTNFEEMKKLADEKQ
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHC
GFIKAMWCGELACEEKLKEEVASRCMPFEQEHLAEECVCCVNKWCIGEKRINALLDRKDE
CCEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCC
KDFGLHNKCNPKSFLVLQFVSCIFKMEG
CCCCCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12721629