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The map label for this gene is ligA

Identifier: 49183320

GI number: 49183320

Start: 313459

End: 315468

Strand: Direct

Name: ligA

Synonym: BAS0292

Alternate gene names: 49183320

Gene position: 313459-315468 (Clockwise)

Preceding gene: 49183319

Following gene: 49183321

Centisome position: 6.0

GC content: 37.96

Gene sequence:

>2010_bases
ATGTCAAAAGAGATAGCAAAAAAACGTATAGAAGAACTACGTGATTTGTTAAATACATTTAACTATCAATATCACGTATT
AGACAATCCTTCTGTTTCTGATGCGGAGTATGACCGTAATATGCAGGAGCTTATAAAATTAGAAGCAGAGAATCCAGAGT
TTATGAGTGAAGACTCTCCCTCTATTCGAGTTGGGGGAACGGTTCTTGATATATTTGAAAAAGTAACACATAAGTCACCG
ATGTTAAGTTTAGGAAATGCATTTAACGAAGGAGATTTACGTGATTTTGACAGACGAGTACGTCAAGGAATTGATGATGC
GAATGTAAGATATATATGCGAATTAAAAATTGACGGACTTGCTGTTTCACTTCATTATGAAAAAGGACGCTTCATTCAAG
GGGCAACACGTGGTGATGGTGTAACAGGTGAAGATATTACCCAAAATTTAAAAACGATTAAAGCAATCCCGCTTCGTTTA
AATGAAGAAGTAACGTTAGAAGCACGAGGCGAAGCTTATATGCCGAAGCGTTCATTCGTTAAGTTAAATGAAGAAAAAGA
GCAAAATGGTGAAGATGTATTTGCGAATCCGCGTAATGCGGCAGCAGGTTCAATCCGCCAACTTGATCCGAAAATTGCAG
CGAAGCGTAACTTATCTATGTTTGTATACGGTCTTGCGAATGTAGAAGAAAAAACAATCCCATCACATAGTGAATCGCTT
GATTACTTAGGTGAACTTGGATTCAAAACAAATCCAAATCGTCGTACATGTGAAACAATTGAAGAAGTTATAGCTTATGT
AGAAGAATGGCAAGAAAAACGTCCGCATCTTGATTATGAGATTGATGGGATCGTTATAAAAGTAGATGATGTTGCTCTTC
AAGAAAGTCTAGGAACTACAGCAAAGAGTCCAAGATGGGCGATTGCTTATAAATTCCCAGCGGAAGAAGTTGTAACAAGA
TTAACGGGCATTGAATTAAGTGTTGGTCGTACAGGAGTTGTAACACCGACTGCAGAGCTAGAGCCAGTGCGAGTGGCTGG
TACGATCGTTCGTCGTGCTTCTTTACATAACGAAGATTTAATTCGTGAAAAAGATATTCGAATTGGTGACTACGTTGTTG
TGAAGAAAGCTGGAGATATTATTCCAGAAGTTGTAAATGTTATTTTTGATAAGCGTACTGGTGGGGAAGAAGAATATCAT
ATGCCAACGCATTGCCCAGCATGTGAGAGTGAACTAGTTCGTTTAGAAGAAGAGGTAGCACTTCGTTGTATAAATCCAAC
TTGTCCTGCTCAAATTCGAGAAGGGTTAATCCATTTCGTTTCAAGAAATGCAATGAATATTGATGGTCTTGGAGAACGTG
TTATTACACAACTCTTTGATGCTGATTATATTCGTACATTTGCGGATTTATATTCGTTGACGAAAGAGCAATTATTACAG
TTAGAACGATTCGGAGAAAAATCAGCAACGAATTTAGTACAAGCAATTGAGAATTCTAAAGAAAACTCATTAGAGCGATT
ATTATTCGGTCTTGGTATTCGCCATGTCGGAGCGAAAGCAGCACGTACATTTGCAGAGCATTTCGAAACGATGGATGCAC
TTGTGAAAGCGACGGAAGAAGAATTAAAAGCAATTAACGAGATTGGTGAAAAAATGGCTCAATCCGTCGTGGCGTATTTT
GATAATGAAGACGTATTAGAGCTATTACAACAATTTAAAGAGTATGGCGTGAATATGACATACAAAGGTATAAAAATTGC
TGATTTACAAAATGTTGAGTCGTACTTTGCAGGAAAAACTGTCGTTTTAACTGGGAAATTAGAAGTTATGGGACGTAGTG
AAGCGAAGAAGAAGATTGAGGCATTAGGTGGAAAAGTAACAGGAAGTGTTAGTAAAAGTACGGATTTAGTTGTCGCAGGT
GAAGCGGCAGGTTCGAAATTAGCACAAGCGGAGAAACATAATGTTGAGGTTTGGAATGAAGAGAGGTTCTTACAAGAGCT
AAATAAGTAA

Upstream 100 bases:

>100_bases
TGCAAAAGAATTAGATATTGCGTTCCCAAGCCCAATTGGTGTTAAACGTTTGTTAGCAAAATTTGCACCTGTGACGAAAC
AATAGGAAAGGAATGAGGAT

Downstream 100 bases:

>100_bases
GAGGTGCAAACTTACCATGAAAAAAATAGCATTAGCGGTATTAAGCCTTGGCCTACTTGTAAGTGGGTGTAGTGCAGGTG
CCGATAAAGATGAAAAAGTG

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 669; Mature: 668

Protein sequence:

>669_residues
MSKEIAKKRIEELRDLLNTFNYQYHVLDNPSVSDAEYDRNMQELIKLEAENPEFMSEDSPSIRVGGTVLDIFEKVTHKSP
MLSLGNAFNEGDLRDFDRRVRQGIDDANVRYICELKIDGLAVSLHYEKGRFIQGATRGDGVTGEDITQNLKTIKAIPLRL
NEEVTLEARGEAYMPKRSFVKLNEEKEQNGEDVFANPRNAAAGSIRQLDPKIAAKRNLSMFVYGLANVEEKTIPSHSESL
DYLGELGFKTNPNRRTCETIEEVIAYVEEWQEKRPHLDYEIDGIVIKVDDVALQESLGTTAKSPRWAIAYKFPAEEVVTR
LTGIELSVGRTGVVTPTAELEPVRVAGTIVRRASLHNEDLIREKDIRIGDYVVVKKAGDIIPEVVNVIFDKRTGGEEEYH
MPTHCPACESELVRLEEEVALRCINPTCPAQIREGLIHFVSRNAMNIDGLGERVITQLFDADYIRTFADLYSLTKEQLLQ
LERFGEKSATNLVQAIENSKENSLERLLFGLGIRHVGAKAARTFAEHFETMDALVKATEEELKAINEIGEKMAQSVVAYF
DNEDVLELLQQFKEYGVNMTYKGIKIADLQNVESYFAGKTVVLTGKLEVMGRSEAKKKIEALGGKVTGSVSKSTDLVVAG
EAAGSKLAQAEKHNVEVWNEERFLQELNK

Sequences:

>Translated_669_residues
MSKEIAKKRIEELRDLLNTFNYQYHVLDNPSVSDAEYDRNMQELIKLEAENPEFMSEDSPSIRVGGTVLDIFEKVTHKSP
MLSLGNAFNEGDLRDFDRRVRQGIDDANVRYICELKIDGLAVSLHYEKGRFIQGATRGDGVTGEDITQNLKTIKAIPLRL
NEEVTLEARGEAYMPKRSFVKLNEEKEQNGEDVFANPRNAAAGSIRQLDPKIAAKRNLSMFVYGLANVEEKTIPSHSESL
DYLGELGFKTNPNRRTCETIEEVIAYVEEWQEKRPHLDYEIDGIVIKVDDVALQESLGTTAKSPRWAIAYKFPAEEVVTR
LTGIELSVGRTGVVTPTAELEPVRVAGTIVRRASLHNEDLIREKDIRIGDYVVVKKAGDIIPEVVNVIFDKRTGGEEEYH
MPTHCPACESELVRLEEEVALRCINPTCPAQIREGLIHFVSRNAMNIDGLGERVITQLFDADYIRTFADLYSLTKEQLLQ
LERFGEKSATNLVQAIENSKENSLERLLFGLGIRHVGAKAARTFAEHFETMDALVKATEEELKAINEIGEKMAQSVVAYF
DNEDVLELLQQFKEYGVNMTYKGIKIADLQNVESYFAGKTVVLTGKLEVMGRSEAKKKIEALGGKVTGSVSKSTDLVVAG
EAAGSKLAQAEKHNVEVWNEERFLQELNK
>Mature_668_residues
SKEIAKKRIEELRDLLNTFNYQYHVLDNPSVSDAEYDRNMQELIKLEAENPEFMSEDSPSIRVGGTVLDIFEKVTHKSPM
LSLGNAFNEGDLRDFDRRVRQGIDDANVRYICELKIDGLAVSLHYEKGRFIQGATRGDGVTGEDITQNLKTIKAIPLRLN
EEVTLEARGEAYMPKRSFVKLNEEKEQNGEDVFANPRNAAAGSIRQLDPKIAAKRNLSMFVYGLANVEEKTIPSHSESLD
YLGELGFKTNPNRRTCETIEEVIAYVEEWQEKRPHLDYEIDGIVIKVDDVALQESLGTTAKSPRWAIAYKFPAEEVVTRL
TGIELSVGRTGVVTPTAELEPVRVAGTIVRRASLHNEDLIREKDIRIGDYVVVKKAGDIIPEVVNVIFDKRTGGEEEYHM
PTHCPACESELVRLEEEVALRCINPTCPAQIREGLIHFVSRNAMNIDGLGERVITQLFDADYIRTFADLYSLTKEQLLQL
ERFGEKSATNLVQAIENSKENSLERLLFGLGIRHVGAKAARTFAEHFETMDALVKATEEELKAINEIGEKMAQSVVAYFD
NEDVLELLQQFKEYGVNMTYKGIKIADLQNVESYFAGKTVVLTGKLEVMGRSEAKKKIEALGGKVTGSVSKSTDLVVAGE
AAGSKLAQAEKHNVEVWNEERFLQELNK

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=671, Percent_Identity=46.9448584202683, Blast_Score=614, Evalue=1e-177,
Organism=Escherichia coli, GI87082305, Length=458, Percent_Identity=24.0174672489083, Blast_Score=107, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_BACAA (C3PBP1)

Other databases:

- EMBL:   CP001598
- RefSeq:   YP_002864923.1
- ProteinModelPortal:   C3PBP1
- EnsemblBacteria:   EBBACT00000126823
- GeneID:   7851780
- GenomeReviews:   CP001598_GR
- KEGG:   bai:BAA_0360
- GeneTree:   EBGT00050000002892
- ProtClustDB:   PRK07956
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 75111; Mature: 74980

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 116-116 BINDING 114-114 BINDING 137-137 BINDING 171-171 BINDING 287-287 BINDING 311-311

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKEIAKKRIEELRDLLNTFNYQYHVLDNPSVSDAEYDRNMQELIKLEAENPEFMSEDSP
CCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHCCHHHHHHHCCCCCCCCCCCCC
SIRVGGTVLDIFEKVTHKSPMLSLGNAFNEGDLRDFDRRVRQGIDDANVRYICELKIDGL
CEEECHHHHHHHHHHHCCCCHHHHCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEEEECCE
AVSLHYEKGRFIQGATRGDGVTGEDITQNLKTIKAIPLRLNEEVTLEARGEAYMPKRSFV
EEEEEECCCCEEECCCCCCCCCHHHHHHHHHHHHHCCEECCCEEEEEECCCCCCCCHHHE
KLNEEKEQNGEDVFANPRNAAAGSIRQLDPKIAAKRNLSMFVYGLANVEEKTIPSHSESL
ECCCHHCCCCCCCCCCCCCCCCCCHHHCCHHHHHHCCCEEEEEEECCCCHHCCCCCHHHH
DYLGELGFKTNPNRRTCETIEEVIAYVEEWQEKRPHLDYEIDGIVIKVDDVALQESLGTT
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECEEEEEECCHHHHHHCCCC
AKSPRWAIAYKFPAEEVVTRLTGIELSVGRTGVVTPTAELEPVRVAGTIVRRASLHNEDL
CCCCCEEEEEECCHHHHHHHHHCCEEECCCCCEECCCCCCCCHHHHHHHHHHHHCCCHHH
IREKDIRIGDYVVVKKAGDIIPEVVNVIFDKRTGGEEEYHMPTHCPACESELVRLEEEVA
HHHCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
LRCINPTCPAQIREGLIHFVSRNAMNIDGLGERVITQLFDADYIRTFADLYSLTKEQLLQ
HEECCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LERFGEKSATNLVQAIENSKENSLERLLFGLGIRHVGAKAARTFAEHFETMDALVKATEE
HHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELKAINEIGEKMAQSVVAYFDNEDVLELLQQFKEYGVNMTYKGIKIADLQNVESYFAGKT
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEECCEEEECHHHHHHHHCCCE
VVLTGKLEVMGRSEAKKKIEALGGKVTGSVSKSTDLVVAGEAAGSKLAQAEKHNVEVWNE
EEEEEEEEECCCHHHHHHHHHHCCCEECCCCCCCCEEEECCCCCHHHHHHHHCCCEEECH
ERFLQELNK
HHHHHHHCC
>Mature Secondary Structure 
SKEIAKKRIEELRDLLNTFNYQYHVLDNPSVSDAEYDRNMQELIKLEAENPEFMSEDSP
CHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHCCHHHHHHHCCCCCCCCCCCCC
SIRVGGTVLDIFEKVTHKSPMLSLGNAFNEGDLRDFDRRVRQGIDDANVRYICELKIDGL
CEEECHHHHHHHHHHHCCCCHHHHCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEEEECCE
AVSLHYEKGRFIQGATRGDGVTGEDITQNLKTIKAIPLRLNEEVTLEARGEAYMPKRSFV
EEEEEECCCCEEECCCCCCCCCHHHHHHHHHHHHHCCEECCCEEEEEECCCCCCCCHHHE
KLNEEKEQNGEDVFANPRNAAAGSIRQLDPKIAAKRNLSMFVYGLANVEEKTIPSHSESL
ECCCHHCCCCCCCCCCCCCCCCCCHHHCCHHHHHHCCCEEEEEEECCCCHHCCCCCHHHH
DYLGELGFKTNPNRRTCETIEEVIAYVEEWQEKRPHLDYEIDGIVIKVDDVALQESLGTT
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECEEEEEECCHHHHHHCCCC
AKSPRWAIAYKFPAEEVVTRLTGIELSVGRTGVVTPTAELEPVRVAGTIVRRASLHNEDL
CCCCCEEEEEECCHHHHHHHHHCCEEECCCCCEECCCCCCCCHHHHHHHHHHHHCCCHHH
IREKDIRIGDYVVVKKAGDIIPEVVNVIFDKRTGGEEEYHMPTHCPACESELVRLEEEVA
HHHCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
LRCINPTCPAQIREGLIHFVSRNAMNIDGLGERVITQLFDADYIRTFADLYSLTKEQLLQ
HEECCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LERFGEKSATNLVQAIENSKENSLERLLFGLGIRHVGAKAARTFAEHFETMDALVKATEE
HHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELKAINEIGEKMAQSVVAYFDNEDVLELLQQFKEYGVNMTYKGIKIADLQNVESYFAGKT
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEECCEEEECHHHHHHHHCCCE
VVLTGKLEVMGRSEAKKKIEALGGKVTGSVSKSTDLVVAGEAAGSKLAQAEKHNVEVWNE
EEEEEEEEECCCHHHHHHHHHHCCCEECCCCCCCCEEEECCCCCHHHHHHHHCCCEEECH
ERFLQELNK
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA