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The map label for this gene is pcrA [H]

Identifier: 49183319

GI number: 49183319

Start: 311188

End: 313443

Strand: Direct

Name: pcrA [H]

Synonym: BAS0291

Alternate gene names: 49183319

Gene position: 311188-313443 (Clockwise)

Preceding gene: 49183318

Following gene: 49183320

Centisome position: 5.95

GC content: 38.61

Gene sequence:

>2256_bases
GTGCAGGCGTATATGAGTATGACAGATAGGTTATTAAATGGTTTAAACCCGCAACAACAAAAAGCAGTACAAACAACGAA
CGGACCACTTCTATTAATGGCAGGCGCAGGTAGTGGTAAAACACGTGTGTTAACACATCGTATTGCGTATTTACTTGGTG
AAAAAGGAGTAGCACCGTGGAATGTACTAGCTATTACCTTTACAAATAAAGCCGCTCGTGAAATGCGCGAGCGTATTGAT
ACACTTGTCGGACCAGAAGCCGAAGATATTTGGATTTCTACGTTCCACTCTATGTGTGTACGTATTTTACGACGTGATAT
CGATCACATTGGTATTAATCGTAACTTTACAATTTTAGATTCGGGTGATCAGTTAACTGTGGTCAAAAAAATTATGAAAG
AGCGCAATATTGATCCGAAGAAATTTGAGCCGCGCTCTATTTTGGCTGGTATTAGTAATGCGAAAAATGAACTGTTATCT
GCGGATAAGTATGCAAAGAAAATTACAATTGCTGATCCATATGAAAAATTAACGAGCGATGTATATACAGAATATCAAAA
ACGTCTTTTGAAAAATAACTCATTAGACTTTGATGATTTAATTATGACGACGATTCAACTATTTGAACGTGTTCCAGAAG
TACTAGAGTTTTATCAGCGCAAATTCCAGTATATTCACGTGGATGAGTATCAAGATACGAACAAAGCGCAGTACCTTCTT
GTTAAACATTTGGCAGCACGTTTTAAAAATTTATGCGTTGTAGGTGACTCAGATCAGTCAATTTATCGCTGGCGTGGTGC
GGACATTTCCAACATTTTGTCATTCGAAAAAGACTATGAGAATGCGCAAGTTATTCTGTTAGAACAAAATTATCGTTCGT
CACAAAATATTTTAAATGCAGCGAATGCTGTTATTGAAAGAAATACGAATCGTAAACCGAAGAAGTTATGGACAGATAAT
GAAGTTGGAAGCAAAATCTCGTACTATCGTGCTGCAACGGAAAAGGACGAGGCGTACTTTGTTGCGAAAAAAATTCGCGA
CGATATTCAAATGGGAAAACGAAAATATACTGATTTTGCAGTGCTATATCGTACAAATGCGCAGTCTCGTATGGTCGAGG
AGATTTTCCTGAAATCTAATATTCCTTACAAAATTGTCGGCGGTACGAAGTTCTACGACCGTAAAGAGATTAAAGACATT
TTGGCGTATTTACGTTTAATTGGGAATCCAGATGATGAGATTAGTTTTGCGCGTATTATTAACGTGCCAAAGCGCGGAAT
TGGTGCGACTTCTATCGACAAAATTATTAATTATGGTGTACAAAATGGAATTTCTTTAACTGCTGTATTCGATGAGATTG
AGCATGTTGGAGTAAGTGCAAAGGTTACAAAGGCAGTAAAAGAATTCGCAGGTTTATTACACAATTGGGTAAATATGCAA
GAGTATTTATCTGTTACAGAATTGGTAGAAGAAGTGATTGAAAAAACAGGCTATCGCGATATGTTGAAAAATGAGCGTAC
GTTAGAAGCAGAAGGTCGTCTGGAAAACTTAGATGAGTTTTTATCTGTTACGCAAACATTTGAATCTCAAAGCGAGGATA
AGAGCCTTGTTGCATTCTTAACAGACTTAGCACTTGTTGCAGATATTGATCGTGTAGATGAAGACCCAACTGCTGGTGAG
GAAGTTATTTTAATGACGATGCACTCAGCGAAGGGGTTAGAATTCCCAGTTGTCTTTATTGTTGGTTTAGAGGAAGGAAT
ATTCCCACATACTCGTTCTCTAATGGAAGAAGATGAAATGCAAGAAGAGCGTCGTCTTGCTTATGTAGGTATTACTCGTG
CGGAAGAAGAGTTGTATTTATCAAATGCACAAATGCGTACTTTATTTGGTAGAACAAGTATGAATGCCGCATCGCGATTT
ATTACAGAAATCCCGACAGAACTAGTAGAATCATTAAATGAAACAGCACCGAAGCGTGAAACTTCGTTTGGTGCAAAAGG
AAGAGTGGCAAGTAGCAGTAAAACGACAACTACAACACGTTCTCGCTCAGCTTTCGCACGTCCTGCAGCTAAGACGACAG
GCGGCGAACAAATTGGCTGGGCAGTAGGTGATAAAGCTTCCCACCAAAAATGGGGAGTCGGTACAGTTATAAGTGTAAAA
GGTGAAGGTGATGCAAAAGAATTAGATATTGCGTTCCCAAGCCCAATTGGTGTTAAACGTTTGTTAGCAAAATTTGCACC
TGTGACGAAACAATAG

Upstream 100 bases:

>100_bases
GGAAATGGCGCAACATGCTGATACAGTAGTTGTTGGAAATATTATTTATGATGATATAAAAGCAGCGTTAAAAACGGTTA
AAGCAGTAAAAGGAGAGTAG

Downstream 100 bases:

>100_bases
GAAAGGAATGAGGATATGTCAAAAGAGATAGCAAAAAAACGTATAGAAGAACTACGTGATTTGTTAAATACATTTAACTA
TCAATATCACGTATTAGACA

Product: ATP-dependent DNA helicase PcrA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 751; Mature: 751

Protein sequence:

>751_residues
MQAYMSMTDRLLNGLNPQQQKAVQTTNGPLLLMAGAGSGKTRVLTHRIAYLLGEKGVAPWNVLAITFTNKAAREMRERID
TLVGPEAEDIWISTFHSMCVRILRRDIDHIGINRNFTILDSGDQLTVVKKIMKERNIDPKKFEPRSILAGISNAKNELLS
ADKYAKKITIADPYEKLTSDVYTEYQKRLLKNNSLDFDDLIMTTIQLFERVPEVLEFYQRKFQYIHVDEYQDTNKAQYLL
VKHLAARFKNLCVVGDSDQSIYRWRGADISNILSFEKDYENAQVILLEQNYRSSQNILNAANAVIERNTNRKPKKLWTDN
EVGSKISYYRAATEKDEAYFVAKKIRDDIQMGKRKYTDFAVLYRTNAQSRMVEEIFLKSNIPYKIVGGTKFYDRKEIKDI
LAYLRLIGNPDDEISFARIINVPKRGIGATSIDKIINYGVQNGISLTAVFDEIEHVGVSAKVTKAVKEFAGLLHNWVNMQ
EYLSVTELVEEVIEKTGYRDMLKNERTLEAEGRLENLDEFLSVTQTFESQSEDKSLVAFLTDLALVADIDRVDEDPTAGE
EVILMTMHSAKGLEFPVVFIVGLEEGIFPHTRSLMEEDEMQEERRLAYVGITRAEEELYLSNAQMRTLFGRTSMNAASRF
ITEIPTELVESLNETAPKRETSFGAKGRVASSSKTTTTTRSRSAFARPAAKTTGGEQIGWAVGDKASHQKWGVGTVISVK
GEGDAKELDIAFPSPIGVKRLLAKFAPVTKQ

Sequences:

>Translated_751_residues
MQAYMSMTDRLLNGLNPQQQKAVQTTNGPLLLMAGAGSGKTRVLTHRIAYLLGEKGVAPWNVLAITFTNKAAREMRERID
TLVGPEAEDIWISTFHSMCVRILRRDIDHIGINRNFTILDSGDQLTVVKKIMKERNIDPKKFEPRSILAGISNAKNELLS
ADKYAKKITIADPYEKLTSDVYTEYQKRLLKNNSLDFDDLIMTTIQLFERVPEVLEFYQRKFQYIHVDEYQDTNKAQYLL
VKHLAARFKNLCVVGDSDQSIYRWRGADISNILSFEKDYENAQVILLEQNYRSSQNILNAANAVIERNTNRKPKKLWTDN
EVGSKISYYRAATEKDEAYFVAKKIRDDIQMGKRKYTDFAVLYRTNAQSRMVEEIFLKSNIPYKIVGGTKFYDRKEIKDI
LAYLRLIGNPDDEISFARIINVPKRGIGATSIDKIINYGVQNGISLTAVFDEIEHVGVSAKVTKAVKEFAGLLHNWVNMQ
EYLSVTELVEEVIEKTGYRDMLKNERTLEAEGRLENLDEFLSVTQTFESQSEDKSLVAFLTDLALVADIDRVDEDPTAGE
EVILMTMHSAKGLEFPVVFIVGLEEGIFPHTRSLMEEDEMQEERRLAYVGITRAEEELYLSNAQMRTLFGRTSMNAASRF
ITEIPTELVESLNETAPKRETSFGAKGRVASSSKTTTTTRSRSAFARPAAKTTGGEQIGWAVGDKASHQKWGVGTVISVK
GEGDAKELDIAFPSPIGVKRLLAKFAPVTKQ
>Mature_751_residues
MQAYMSMTDRLLNGLNPQQQKAVQTTNGPLLLMAGAGSGKTRVLTHRIAYLLGEKGVAPWNVLAITFTNKAAREMRERID
TLVGPEAEDIWISTFHSMCVRILRRDIDHIGINRNFTILDSGDQLTVVKKIMKERNIDPKKFEPRSILAGISNAKNELLS
ADKYAKKITIADPYEKLTSDVYTEYQKRLLKNNSLDFDDLIMTTIQLFERVPEVLEFYQRKFQYIHVDEYQDTNKAQYLL
VKHLAARFKNLCVVGDSDQSIYRWRGADISNILSFEKDYENAQVILLEQNYRSSQNILNAANAVIERNTNRKPKKLWTDN
EVGSKISYYRAATEKDEAYFVAKKIRDDIQMGKRKYTDFAVLYRTNAQSRMVEEIFLKSNIPYKIVGGTKFYDRKEIKDI
LAYLRLIGNPDDEISFARIINVPKRGIGATSIDKIINYGVQNGISLTAVFDEIEHVGVSAKVTKAVKEFAGLLHNWVNMQ
EYLSVTELVEEVIEKTGYRDMLKNERTLEAEGRLENLDEFLSVTQTFESQSEDKSLVAFLTDLALVADIDRVDEDPTAGE
EVILMTMHSAKGLEFPVVFIVGLEEGIFPHTRSLMEEDEMQEERRLAYVGITRAEEELYLSNAQMRTLFGRTSMNAASRF
ITEIPTELVESLNETAPKRETSFGAKGRVASSSKTTTTTRSRSAFARPAAKTTGGEQIGWAVGDKASHQKWGVGTVISVK
GEGDAKELDIAFPSPIGVKRLLAKFAPVTKQ

Specific function: DNA helicase used for plasmid rolling-circle replication and also involved in UV repair [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=745, Percent_Identity=39.1946308724832, Blast_Score=529, Evalue=1e-151,
Organism=Escherichia coli, GI48994965, Length=643, Percent_Identity=39.6578538102644, Blast_Score=423, Evalue=1e-119,
Organism=Escherichia coli, GI1787196, Length=347, Percent_Identity=27.9538904899135, Blast_Score=101, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6322369, Length=774, Percent_Identity=29.0697674418605, Blast_Score=244, Evalue=3e-65,
Organism=Saccharomyces cerevisiae, GI6324477, Length=688, Percent_Identity=23.1104651162791, Blast_Score=107, Evalue=6e-24,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 84965; Mature: 84965

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAYMSMTDRLLNGLNPQQQKAVQTTNGPLLLMAGAGSGKTRVLTHRIAYLLGEKGVAPW
CCHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCE
NVLAITFTNKAAREMRERIDTLVGPEAEDIWISTFHSMCVRILRRDIDHIGINRNFTILD
EEEEEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
SGDQLTVVKKIMKERNIDPKKFEPRSILAGISNAKNELLSADKYAKKITIADPYEKLTSD
CCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCEEEECCHHHHHHHH
VYTEYQKRLLKNNSLDFDDLIMTTIQLFERVPEVLEFYQRKFQYIHVDEYQDTNKAQYLL
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCHHHHHH
VKHLAARFKNLCVVGDSDQSIYRWRGADISNILSFEKDYENAQVILLEQNYRSSQNILNA
HHHHHHHHHCEEEECCCCCCEEEECCCCHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHH
ANAVIERNTNRKPKKLWTDNEVGSKISYYRAATEKDEAYFVAKKIRDDIQMGKRKYTDFA
HHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCEE
VLYRTNAQSRMVEEIFLKSNIPYKIVGGTKFYDRKEIKDILAYLRLIGNPDDEISFARII
EEEECCCHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHH
NVPKRGIGATSIDKIINYGVQNGISLTAVFDEIEHVGVSAKVTKAVKEFAGLLHNWVNMQ
CCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCHH
EYLSVTELVEEVIEKTGYRDMLKNERTLEAEGRLENLDEFLSVTQTFESQSEDKSLVAFL
HHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHH
TDLALVADIDRVDEDPTAGEEVILMTMHSAKGLEFPVVFIVGLEEGIFPHTRSLMEEDEM
HHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCEEEEEECCCCCCHHHHHHHHHHHH
QEERRLAYVGITRAEEELYLSNAQMRTLFGRTSMNAASRFITEIPTELVESLNETAPKRE
HHHHCEEEEEEECCHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
TSFGAKGRVASSSKTTTTTRSRSAFARPAAKTTGGEQIGWAVGDKASHQKWGVGTVISVK
CCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCEECEECCCCCCCCCCCCCEEEEEE
GEGDAKELDIAFPSPIGVKRLLAKFAPVTKQ
CCCCCCEEEEECCCCCCHHHHHHHHCCCCCC
>Mature Secondary Structure
MQAYMSMTDRLLNGLNPQQQKAVQTTNGPLLLMAGAGSGKTRVLTHRIAYLLGEKGVAPW
CCHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCE
NVLAITFTNKAAREMRERIDTLVGPEAEDIWISTFHSMCVRILRRDIDHIGINRNFTILD
EEEEEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
SGDQLTVVKKIMKERNIDPKKFEPRSILAGISNAKNELLSADKYAKKITIADPYEKLTSD
CCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCEEEECCHHHHHHHH
VYTEYQKRLLKNNSLDFDDLIMTTIQLFERVPEVLEFYQRKFQYIHVDEYQDTNKAQYLL
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCHHHHHH
VKHLAARFKNLCVVGDSDQSIYRWRGADISNILSFEKDYENAQVILLEQNYRSSQNILNA
HHHHHHHHHCEEEECCCCCCEEEECCCCHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHH
ANAVIERNTNRKPKKLWTDNEVGSKISYYRAATEKDEAYFVAKKIRDDIQMGKRKYTDFA
HHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCEE
VLYRTNAQSRMVEEIFLKSNIPYKIVGGTKFYDRKEIKDILAYLRLIGNPDDEISFARII
EEEECCCHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHH
NVPKRGIGATSIDKIINYGVQNGISLTAVFDEIEHVGVSAKVTKAVKEFAGLLHNWVNMQ
CCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCHH
EYLSVTELVEEVIEKTGYRDMLKNERTLEAEGRLENLDEFLSVTQTFESQSEDKSLVAFL
HHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHH
TDLALVADIDRVDEDPTAGEEVILMTMHSAKGLEFPVVFIVGLEEGIFPHTRSLMEEDEM
HHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCEEEEEECCCCCCHHHHHHHHHHHH
QEERRLAYVGITRAEEELYLSNAQMRTLFGRTSMNAASRFITEIPTELVESLNETAPKRE
HHHHCEEEEEEECCHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
TSFGAKGRVASSSKTTTTTRSRSAFARPAAKTTGGEQIGWAVGDKASHQKWGVGTVISVK
CCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCEECEECCCCCCCCCCCCCEEEEEE
GEGDAKELDIAFPSPIGVKRLLAKFAPVTKQ
CCCCCCEEEEECCCCCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9701819; 9384377 [H]