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The map label for this gene is purH

Identifier: 49183313

GI number: 49183313

Start: 305586

End: 307121

Strand: Direct

Name: purH

Synonym: BAS0285

Alternate gene names: 49183313

Gene position: 305586-307121 (Clockwise)

Preceding gene: 49183312

Following gene: 49183314

Centisome position: 5.84

GC content: 38.02

Gene sequence:

>1536_bases
ATGAAAAAGCGTGCATTAGTAAGTGTTTCAGATAAAACAGGAGTAGTAGAATTTGTTAAAGGTTTACTAGAACAAGGGAT
CGAAGTTATATCAACAGGTGGTACGAAAAAATTACTAGAAGAAAACGGCTTACAAGTAATCGGTATTTCTGAAGTAACTG
GTTTCCCAGAAATTATGGATGGTCGTGTGAAAACATTACATCCAAATATTCATGGTGGTCTATTAGCAGTTCGTGATAAT
GAAACACATGTAGCGCAAATGAATGAATTAGGTATGGAGCCGATTGATTTTGTTGTCGTTAACTTATACCCATTCAAAGA
AACGATCGCTAAGCCTGATGTAACATTTGCTGATGCAATTGAAAATATTGATATCGGTGGCCCGACAATGATTCGCTCTG
CTGCGAAAAATCATAAATTCGTATCTGTAATTGTAGATCCAGTAGATTATGATGTTGTATTAGCTGAATTAAAAGAGAAC
GGTGAAGTAGCAGAGGAAACAAAACGTAAACTAGCAGCGAAAGTATTCCGTCATACAGCAGCGTATGATGCGTTAATTTC
TAACTACTTAACAGAGCAAATGGGTGAAGAAAGTCCAGAAACATTAACTGTGACATTTGAGAAAAAGCAAGACTTACGTT
ATGGCGAGAACCCACATCAAAAAGCAACTTTCTATAAAGCGCCATTCGCAGCAACTTCTTCTGTTGCATACGCAGAACAA
TTACATGGCAAGGAATTATCGTATAACAATATTAACGATGCAGATGCAGCGCTTAGTATCGTGAAAGAATTTACAGAACC
AGCAGTAGTAGCGGTAAAACATATGAATCCATGTGGTGTTGGAGTAGGTACGGATATTCATGAAGCATACACACGTGCTT
ATGAAGCGGATCCAGTATCCATTTTTGGAGGAATTATTGCAGCGAATCGTGAAATTGATAAAGCTACAGCTGAAAAGTTA
CACGAAATTTTCTTAGAGATTATTATCGCACCTTCTTTCTCGAAAGAAGCTTTAGAAGTACTGCAAAGTAAGAAAAACTT
ACGTTTATTAACTGTAAATATTGAAAAAGCGACAAGCGCAAGCAAAAAATTAACTTCTGTACAAGGTGGCCTTCTCGTTC
AAGAGGAAGATACGTTATCATTAGATGAAAGTACAATTTCAATTCCAACGAAACGTGAACCTTCAGAGCAAGAATGGAAA
GATTTAAAACTAGCTTGGAAAGTTGTAAAGCATGTGAAATCAAATGCAATTGTTTTAGCAAAAGATGATATGACAATTGG
TGTCGGTGCTGGACAGATGAACCGTGTAGGTTCTGCAAAAATCGCAATTACACAAGCTGGCGAAAAAGCACAAGGTAGCG
CACTTGCATCTGATGCTTTCTTCCCAATGCCAGATACAGTAGAAGAAGCAGCAAAAGCAGGAATTACGGCAATCATTCAA
CCAGGCGGATCAATCCGTGACGAAGATTCTATTAAAGTGGCGGATACGTATGGGATTGCTATGGTGTTCACTGGCGTACG
TCATTTCAAACACTAA

Upstream 100 bases:

>100_bases
GCAAGTTGAACATAAATTATACGTAAATACAGTGAATCAAATTGTTCAATCTACGAAAGAACCAACTGTTAATTAACATA
CAACCAGGGGTGAAGGATAA

Downstream 100 bases:

>100_bases
TAGAGGAAGTTTAAAAAGTCCGGTTAAGATAACCTTCCTTTTTAAACTTTGATTGATATGAAAAAACAAACTAGGAGCAT
AGCTTCTAGTTTGTACTTAT

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase

Number of amino acids: Translated: 511; Mature: 511

Protein sequence:

>511_residues
MKKRALVSVSDKTGVVEFVKGLLEQGIEVISTGGTKKLLEENGLQVIGISEVTGFPEIMDGRVKTLHPNIHGGLLAVRDN
ETHVAQMNELGMEPIDFVVVNLYPFKETIAKPDVTFADAIENIDIGGPTMIRSAAKNHKFVSVIVDPVDYDVVLAELKEN
GEVAEETKRKLAAKVFRHTAAYDALISNYLTEQMGEESPETLTVTFEKKQDLRYGENPHQKATFYKAPFAATSSVAYAEQ
LHGKELSYNNINDADAALSIVKEFTEPAVVAVKHMNPCGVGVGTDIHEAYTRAYEADPVSIFGGIIAANREIDKATAEKL
HEIFLEIIIAPSFSKEALEVLQSKKNLRLLTVNIEKATSASKKLTSVQGGLLVQEEDTLSLDESTISIPTKREPSEQEWK
DLKLAWKVVKHVKSNAIVLAKDDMTIGVGAGQMNRVGSAKIAITQAGEKAQGSALASDAFFPMPDTVEEAAKAGITAIIQ
PGGSIRDEDSIKVADTYGIAMVFTGVRHFKH

Sequences:

>Translated_511_residues
MKKRALVSVSDKTGVVEFVKGLLEQGIEVISTGGTKKLLEENGLQVIGISEVTGFPEIMDGRVKTLHPNIHGGLLAVRDN
ETHVAQMNELGMEPIDFVVVNLYPFKETIAKPDVTFADAIENIDIGGPTMIRSAAKNHKFVSVIVDPVDYDVVLAELKEN
GEVAEETKRKLAAKVFRHTAAYDALISNYLTEQMGEESPETLTVTFEKKQDLRYGENPHQKATFYKAPFAATSSVAYAEQ
LHGKELSYNNINDADAALSIVKEFTEPAVVAVKHMNPCGVGVGTDIHEAYTRAYEADPVSIFGGIIAANREIDKATAEKL
HEIFLEIIIAPSFSKEALEVLQSKKNLRLLTVNIEKATSASKKLTSVQGGLLVQEEDTLSLDESTISIPTKREPSEQEWK
DLKLAWKVVKHVKSNAIVLAKDDMTIGVGAGQMNRVGSAKIAITQAGEKAQGSALASDAFFPMPDTVEEAAKAGITAIIQ
PGGSIRDEDSIKVADTYGIAMVFTGVRHFKH
>Mature_511_residues
MKKRALVSVSDKTGVVEFVKGLLEQGIEVISTGGTKKLLEENGLQVIGISEVTGFPEIMDGRVKTLHPNIHGGLLAVRDN
ETHVAQMNELGMEPIDFVVVNLYPFKETIAKPDVTFADAIENIDIGGPTMIRSAAKNHKFVSVIVDPVDYDVVLAELKEN
GEVAEETKRKLAAKVFRHTAAYDALISNYLTEQMGEESPETLTVTFEKKQDLRYGENPHQKATFYKAPFAATSSVAYAEQ
LHGKELSYNNINDADAALSIVKEFTEPAVVAVKHMNPCGVGVGTDIHEAYTRAYEADPVSIFGGIIAANREIDKATAEKL
HEIFLEIIIAPSFSKEALEVLQSKKNLRLLTVNIEKATSASKKLTSVQGGLLVQEEDTLSLDESTISIPTKREPSEQEWK
DLKLAWKVVKHVKSNAIVLAKDDMTIGVGAGQMNRVGSAKIAITQAGEKAQGSALASDAFFPMPDTVEEAAKAGITAIIQ
PGGSIRDEDSIKVADTYGIAMVFTGVRHFKH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family

Homologues:

Organism=Homo sapiens, GI20127454, Length=481, Percent_Identity=37.4220374220374, Blast_Score=274, Evalue=2e-73,
Organism=Escherichia coli, GI1790439, Length=524, Percent_Identity=53.0534351145038, Blast_Score=550, Evalue=1e-157,
Organism=Caenorhabditis elegans, GI71985564, Length=484, Percent_Identity=34.0909090909091, Blast_Score=253, Evalue=1e-67,
Organism=Caenorhabditis elegans, GI71985574, Length=312, Percent_Identity=26.9230769230769, Blast_Score=90, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6323768, Length=478, Percent_Identity=35.3556485355649, Blast_Score=282, Evalue=9e-77,
Organism=Saccharomyces cerevisiae, GI6323056, Length=470, Percent_Identity=35.7446808510638, Blast_Score=281, Evalue=1e-76,
Organism=Drosophila melanogaster, GI24649832, Length=481, Percent_Identity=38.6694386694387, Blast_Score=293, Evalue=3e-79,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PUR9_BACAA (C3PBN4)

Other databases:

- EMBL:   CP001598
- RefSeq:   YP_002864916.1
- ProteinModelPortal:   C3PBN4
- SMR:   C3PBN4
- EnsemblBacteria:   EBBACT00000126365
- GeneID:   7849956
- GenomeReviews:   CP001598_GR
- KEGG:   bai:BAA_0353
- GeneTree:   EBGT00050000002320
- ProtClustDB:   PRK00881
- HAMAP:   MF_00139
- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607
- Gene3D:   G3DSA:3.40.50.1380
- PANTHER:   PTHR11692
- PIRSF:   PIRSF000414
- SMART:   SM00798
- SMART:   SM00851
- TIGRFAMs:   TIGR00355

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS; SSF53927 Cytidine_deaminase-like; SSF52335 MGS-like_dom

EC number: =2.1.2.3; =3.5.4.10

Molecular weight: Translated: 55517; Mature: 55517

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKRALVSVSDKTGVVEFVKGLLEQGIEVISTGGTKKLLEENGLQVIGISEVTGFPEIMD
CCCCEEEEECCCCCHHHHHHHHHHCCHHEEECCCHHHHHHHCCCEEEEEHHHCCCHHHHC
GRVKTLHPNIHGGLLAVRDNETHVAQMNELGMEPIDFVVVNLYPFKETIAKPDVTFADAI
CCEEEECCCCCCCEEEEECCCHHHHHHHHCCCCCEEEEEEEEEECHHHHCCCCCHHHHHH
ENIDIGGPTMIRSAAKNHKFVSVIVDPVDYDVVLAELKENGEVAEETKRKLAAKVFRHTA
HCCCCCCHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
AYDALISNYLTEQMGEESPETLTVTFEKKQDLRYGENPHQKATFYKAPFAATSSVAYAEQ
HHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHH
LHGKELSYNNINDADAALSIVKEFTEPAVVAVKHMNPCGVGVGTDIHEAYTRAYEADPVS
HCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHCCCCCHH
IFGGIIAANREIDKATAEKLHEIFLEIIIAPSFSKEALEVLQSKKNLRLLTVNIEKATSA
HHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEEEEEHHHHCH
SKKLTSVQGGLLVQEEDTLSLDESTISIPTKREPSEQEWKDLKLAWKVVKHVKSNAIVLA
HHHHHHHCCCEEEECCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEE
KDDMTIGVGAGQMNRVGSAKIAITQAGEKAQGSALASDAFFPMPDTVEEAAKAGITAIIQ
ECCEEEEECCCCCCCCCCEEEEEEECCCCCCCCEECCCCCCCCCHHHHHHHHCCCEEEEC
PGGSIRDEDSIKVADTYGIAMVFTGVRHFKH
CCCCCCCCCCEEEEHHCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKKRALVSVSDKTGVVEFVKGLLEQGIEVISTGGTKKLLEENGLQVIGISEVTGFPEIMD
CCCCEEEEECCCCCHHHHHHHHHHCCHHEEECCCHHHHHHHCCCEEEEEHHHCCCHHHHC
GRVKTLHPNIHGGLLAVRDNETHVAQMNELGMEPIDFVVVNLYPFKETIAKPDVTFADAI
CCEEEECCCCCCCEEEEECCCHHHHHHHHCCCCCEEEEEEEEEECHHHHCCCCCHHHHHH
ENIDIGGPTMIRSAAKNHKFVSVIVDPVDYDVVLAELKENGEVAEETKRKLAAKVFRHTA
HCCCCCCHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
AYDALISNYLTEQMGEESPETLTVTFEKKQDLRYGENPHQKATFYKAPFAATSSVAYAEQ
HHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHH
LHGKELSYNNINDADAALSIVKEFTEPAVVAVKHMNPCGVGVGTDIHEAYTRAYEADPVS
HCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHCCCCCHH
IFGGIIAANREIDKATAEKLHEIFLEIIIAPSFSKEALEVLQSKKNLRLLTVNIEKATSA
HHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEEEEEHHHHCH
SKKLTSVQGGLLVQEEDTLSLDESTISIPTKREPSEQEWKDLKLAWKVVKHVKSNAIVLA
HHHHHHHCCCEEEECCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEE
KDDMTIGVGAGQMNRVGSAKIAITQAGEKAQGSALASDAFFPMPDTVEEAAKAGITAIIQ
ECCEEEEECCCCCCCCCCEEEEEEECCCCCCCCEECCCCCCCCCHHHHHHHHCCCEEEEC
PGGSIRDEDSIKVADTYGIAMVFTGVRHFKH
CCCCCCCCCCEEEEHHCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA