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The map label for this gene is purN [H]

Identifier: 49183312

GI number: 49183312

Start: 304974

End: 305561

Strand: Direct

Name: purN [H]

Synonym: BAS0284

Alternate gene names: 49183312

Gene position: 304974-305561 (Clockwise)

Preceding gene: 49183311

Following gene: 49183313

Centisome position: 5.83

GC content: 36.39

Gene sequence:

>588_bases
ATGAGTAGATTAGCAGTTTTTGCTTCTGGAAGCGGATCTAACTTTCAATCTCTCGTTAATGCGGTAGAAGAAAAAAGATT
GGATGCAGAAATTAGTTTATTAGTATGTGATAAACCAGAAGCACGCGCTGTTGGGCGAGCGAATTATCATCATATTCCGT
GTTTTGCTTTTTCAGCGAAAGCATATGAGTCAAAAGAAGCATTTGAAAAAGAAATATTAAACAAGCTAGAAGAGTATGAA
ATTGATTATGTTATTTTAGCTGGATATATGCGTTTAATCGGGCCGACTTTACTAGAAGCTTACGGCGGAAGGATTATTAA
TATTCATCCATCATTATTACCAAGTTTTCCAGGTAAGGATGCTGTCGGCCAAGCGTTAGAAGCGGGTGTGAAAGTAACTG
GAGTAACGATTCATTATGTAGATGCAGGTATGGATACAGGGCCAATTATTGCGCAAGAAGCAGTAGTTGTTTCTGAAGGT
GATACGAGAGAAAGCTTACAAAAGAAAATTCAGCAAGTTGAACATAAATTATACGTAAATACAGTGAATCAAATTGTTCA
ATCTACGAAAGAACCAACTGTTAATTAA

Upstream 100 bases:

>100_bases
AAGATATCGTCCGTCTCCTTGAAGAGCAAGGGGAAACAGCTCGTATTATTGGACGTACTGTACAAGGAGCTGGCGTTACC
TTCAATGGGGGCAAAGCACT

Downstream 100 bases:

>100_bases
CATACAACCAGGGGTGAAGGATAAATGAAAAAGCGTGCATTAGTAAGTGTTTCAGATAAAACAGGAGTAGTAGAATTTGT
TAAAGGTTTACTAGAACAAG

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 195; Mature: 194

Protein sequence:

>195_residues
MSRLAVFASGSGSNFQSLVNAVEEKRLDAEISLLVCDKPEARAVGRANYHHIPCFAFSAKAYESKEAFEKEILNKLEEYE
IDYVILAGYMRLIGPTLLEAYGGRIINIHPSLLPSFPGKDAVGQALEAGVKVTGVTIHYVDAGMDTGPIIAQEAVVVSEG
DTRESLQKKIQQVEHKLYVNTVNQIVQSTKEPTVN

Sequences:

>Translated_195_residues
MSRLAVFASGSGSNFQSLVNAVEEKRLDAEISLLVCDKPEARAVGRANYHHIPCFAFSAKAYESKEAFEKEILNKLEEYE
IDYVILAGYMRLIGPTLLEAYGGRIINIHPSLLPSFPGKDAVGQALEAGVKVTGVTIHYVDAGMDTGPIIAQEAVVVSEG
DTRESLQKKIQQVEHKLYVNTVNQIVQSTKEPTVN
>Mature_194_residues
SRLAVFASGSGSNFQSLVNAVEEKRLDAEISLLVCDKPEARAVGRANYHHIPCFAFSAKAYESKEAFEKEILNKLEEYEI
DYVILAGYMRLIGPTLLEAYGGRIINIHPSLLPSFPGKDAVGQALEAGVKVTGVTIHYVDAGMDTGPIIAQEAVVVSEGD
TRESLQKKIQQVEHKLYVNTVNQIVQSTKEPTVN

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=188, Percent_Identity=39.3617021276596, Blast_Score=150, Evalue=6e-37,
Organism=Homo sapiens, GI209869995, Length=188, Percent_Identity=39.3617021276596, Blast_Score=150, Evalue=6e-37,
Organism=Homo sapiens, GI209869993, Length=188, Percent_Identity=39.3617021276596, Blast_Score=150, Evalue=6e-37,
Organism=Escherichia coli, GI1788846, Length=175, Percent_Identity=35.4285714285714, Blast_Score=138, Evalue=2e-34,
Organism=Escherichia coli, GI1787483, Length=172, Percent_Identity=30.2325581395349, Blast_Score=92, Evalue=2e-20,
Organism=Escherichia coli, GI1789683, Length=118, Percent_Identity=29.6610169491525, Blast_Score=63, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17567511, Length=180, Percent_Identity=36.6666666666667, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6320616, Length=213, Percent_Identity=25.8215962441315, Blast_Score=75, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24582400, Length=189, Percent_Identity=37.5661375661376, Blast_Score=145, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 21337; Mature: 21205

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLAVFASGSGSNFQSLVNAVEEKRLDAEISLLVCDKPEARAVGRANYHHIPCFAFSAK
CCCEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEECCH
AYESKEAFEKEILNKLEEYEIDYVILAGYMRLIGPTLLEAYGGRIINIHPSLLPSFPGKD
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCEEEEECHHHCCCCCCCH
AVGQALEAGVKVTGVTIHYVDAGMDTGPIIAQEAVVVSEGDTRESLQKKIQQVEHKLYVN
HHHHHHHCCCEEEEEEEEEEECCCCCCCCEECCEEEEECCCCHHHHHHHHHHHHHHHHHH
TVNQIVQSTKEPTVN
HHHHHHHHCCCCCCC
>Mature Secondary Structure 
SRLAVFASGSGSNFQSLVNAVEEKRLDAEISLLVCDKPEARAVGRANYHHIPCFAFSAK
CCEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEECCH
AYESKEAFEKEILNKLEEYEIDYVILAGYMRLIGPTLLEAYGGRIINIHPSLLPSFPGKD
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCEEEEECHHHCCCCCCCH
AVGQALEAGVKVTGVTIHYVDAGMDTGPIIAQEAVVVSEGDTRESLQKKIQQVEHKLYVN
HHHHHHHCCCEEEEEEEEEEECCCCCCCCEECCEEEEECCCCHHHHHHHHHHHHHHHHHH
TVNQIVQSTKEPTVN
HHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377 [H]