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The map label for this gene is ispD

Identifier: 49183119

GI number: 49183119

Start: 95963

End: 96643

Strand: Direct

Name: ispD

Synonym: BAS0085

Alternate gene names: 49183119

Gene position: 95963-96643 (Clockwise)

Preceding gene: 49183118

Following gene: 49183120

Centisome position: 1.84

GC content: 39.35

Gene sequence:

>681_bases
ATGTATACATTAATTATTCCAGCAGCTGGTCAGGGAAAGCGGATGGGTGCTGGCAAAAATAAGTTGTTCTTACTTATTAA
TGAAGTACCGATTATTGTGCATACGTTACGTGCTTTTGAAAAGGATAAAGCATGTAAAAATATTATTATGGCAATTAACG
AAGAAGAGCGTCCATATTTTGAAGAGTTAATGCAGAAGTATCCGGTTGAAAAGCCAGTACAATTTATTCAAGGTGGAGCT
GAAAGACAAGATAGTGTATATAATGCGATTCAGCATACGACTGATGTTGAGTATGTTCTTGTACATGACGGTGCGCGCCC
GTTCGTAACGAATAAAGTAATCCAAGATGTATTAACTGCAGCAGAAAAATATGGAGCTTCCATTTGTGCGGTGCCAGTGA
AAGATACTGTTAAGAAAGTAGAGCAGGGTGTTGTTGTCGAAACGGTAGAAAGATCGCAACTTAAAGCTGTACAAACACCG
CAAGGGTTCTCTGTTTCGCTTTTGTTAGAAGCTCATAGAAGTGCGAAGCAGAGTTGTTTCCTTGGTACAGATGATGCAAG
TCTCGTAGAACGTATCGGGAAGCAAGTAGGTGTAGTAGAGGGGAGTTACTATAATATTAAAGTGACGACCCCAGAGGATT
TACTAATTGCTGAAAGTTTTCTTCACGTTCAAAAGAAATGA

Upstream 100 bases:

>100_bases
GATGTACTCGTTACGAGTGTGTTACAAACATCGGCGGGGCGCATGATTTTTGCCAAACGTAAATTATTAGAAAAAGCATT
GTAAGTAGAGGATTATTAAT

Downstream 100 bases:

>100_bases
TAGCAATGATTATAGCGAAGAAAAGCTCGGCAACGGCCGGGCTTTTCTTTATAAGGATAGCGATATAATATAGAATCATA
AAGCTCAGTAGTTTAGCTCG

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MYTLIIPAAGQGKRMGAGKNKLFLLINEVPIIVHTLRAFEKDKACKNIIMAINEEERPYFEELMQKYPVEKPVQFIQGGA
ERQDSVYNAIQHTTDVEYVLVHDGARPFVTNKVIQDVLTAAEKYGASICAVPVKDTVKKVEQGVVVETVERSQLKAVQTP
QGFSVSLLLEAHRSAKQSCFLGTDDASLVERIGKQVGVVEGSYYNIKVTTPEDLLIAESFLHVQKK

Sequences:

>Translated_226_residues
MYTLIIPAAGQGKRMGAGKNKLFLLINEVPIIVHTLRAFEKDKACKNIIMAINEEERPYFEELMQKYPVEKPVQFIQGGA
ERQDSVYNAIQHTTDVEYVLVHDGARPFVTNKVIQDVLTAAEKYGASICAVPVKDTVKKVEQGVVVETVERSQLKAVQTP
QGFSVSLLLEAHRSAKQSCFLGTDDASLVERIGKQVGVVEGSYYNIKVTTPEDLLIAESFLHVQKK
>Mature_226_residues
MYTLIIPAAGQGKRMGAGKNKLFLLINEVPIIVHTLRAFEKDKACKNIIMAINEEERPYFEELMQKYPVEKPVQFIQGGA
ERQDSVYNAIQHTTDVEYVLVHDGARPFVTNKVIQDVLTAAEKYGASICAVPVKDTVKKVEQGVVVETVERSQLKAVQTP
QGFSVSLLLEAHRSAKQSCFLGTDDASLVERIGKQVGVVEGSYYNIKVTTPEDLLIAESFLHVQKK

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP)

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family

Homologues:

Organism=Homo sapiens, GI157412259, Length=229, Percent_Identity=31.0043668122271, Blast_Score=102, Evalue=2e-22,
Organism=Homo sapiens, GI157671913, Length=132, Percent_Identity=33.3333333333333, Blast_Score=81, Evalue=9e-16,
Organism=Escherichia coli, GI1789104, Length=220, Percent_Identity=34.5454545454545, Blast_Score=119, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPD_BACAH (A0R8F7)

Other databases:

- EMBL:   CP000485
- RefSeq:   YP_893007.1
- ProteinModelPortal:   A0R8F7
- SMR:   A0R8F7
- STRING:   A0R8F7
- EnsemblBacteria:   EBBACT00000067256
- GeneID:   4542654
- GenomeReviews:   CP000485_GR
- KEGG:   btl:BALH_0085
- eggNOG:   COG1211
- GeneTree:   EBGT00050000001852
- HOGENOM:   HBG672839
- OMA:   PSNIKVT
- ProtClustDB:   PRK00155
- BioCyc:   BTHU412694:BALH_0085-MONOMER
- HAMAP:   MF_00108
- InterPro:   IPR001228
- InterPro:   IPR018294
- TIGRFAMs:   TIGR00453

Pfam domain/function: PF01128 IspD

EC number: =2.7.7.60

Molecular weight: Translated: 25121; Mature: 25121

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYTLIIPAAGQGKRMGAGKNKLFLLINEVPIIVHTLRAFEKDKACKNIIMAINEEERPYF
CEEEEEECCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
EELMQKYPVEKPVQFIQGGAERQDSVYNAIQHTTDVEYVLVHDGARPFVTNKVIQDVLTA
HHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHH
AEKYGASICAVPVKDTVKKVEQGVVVETVERSQLKAVQTPQGFSVSLLLEAHRSAKQSCF
HHHHCCEEEEECHHHHHHHHHCCHHEEHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHEE
LGTDDASLVERIGKQVGVVEGSYYNIKVTTPEDLLIAESFLHVQKK
CCCCCHHHHHHHHHHHCEECCCEEEEEEECCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MYTLIIPAAGQGKRMGAGKNKLFLLINEVPIIVHTLRAFEKDKACKNIIMAINEEERPYF
CEEEEEECCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
EELMQKYPVEKPVQFIQGGAERQDSVYNAIQHTTDVEYVLVHDGARPFVTNKVIQDVLTA
HHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHH
AEKYGASICAVPVKDTVKKVEQGVVVETVERSQLKAVQTPQGFSVSLLLEAHRSAKQSCF
HHHHCCEEEEECHHHHHHHHHCCHHEEHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHEE
LGTDDASLVERIGKQVGVVEGSYYNIKVTTPEDLLIAESFLHVQKK
CCCCCHHHHHHHHHHHCEECCCEEEEEEECCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA