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The map label for this gene is ispF

Identifier: 49183120

GI number: 49183120

Start: 96758

End: 97234

Strand: Direct

Name: ispF

Synonym: BAS0086

Alternate gene names: 49183120

Gene position: 96758-97234 (Clockwise)

Preceding gene: 49183119

Following gene: 49183121

Centisome position: 1.85

GC content: 37.95

Gene sequence:

>477_bases
ATGTTTCGAATTGGACAAGGTTTTGATGTGCATGAATTCGCGGAAGGTAGACCGTTAATTATTGGTGGAATTACAATTCC
ACATGAGAAAGGATTAATCGGTCACTCTGATGCAGACGTATTATTACATACAATCGCGGATGCGTGTTTAGGTGCAATTG
CAGCAGGAGATATCGGGAAACATTTCCCTGATACAGACCCCGCCTTTAAAGATGCTGATTCAGCTGTATTGTTGCAAAAG
GTTTGGGAGTTTGTGCGTGAACAAGGTTATGAATTAGGGAATTTAGATTGTACAATTATTGCTCAAAAGCCAAAAATGGC
ACCACATATTGAAAGTATGCGCAAACGCATTAGTGAGCTATTAGAAACGTCTATCGATAATATCAATGTAAAGGCAACAA
CAACAGAAAAATTAGGGTTTACAGGTAGAGAAGAAGGAATTGCTTCTCAAGCAGTTGTTTTATTACAGAAAAAATAA

Upstream 100 bases:

>100_bases
GCGAAGAAAAGCTCGGCAACGGCCGGGCTTTTCTTTATAAGGATAGCGATATAATATAGAATCATAAAGCTCAGTAGTTT
AGCTCGAGGAGGATGTAAAA

Downstream 100 bases:

>100_bases
TGGTTTTTAATTCGTAAGATGGATAATCACATTTTTTTGGTGTACAATTATAGAATGTGTTGACATTAAGAATGGAAGGT
GTACCAATTATGGAAAAGCA

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 158; Mature: 158

Protein sequence:

>158_residues
MFRIGQGFDVHEFAEGRPLIIGGITIPHEKGLIGHSDADVLLHTIADACLGAIAAGDIGKHFPDTDPAFKDADSAVLLQK
VWEFVREQGYELGNLDCTIIAQKPKMAPHIESMRKRISELLETSIDNINVKATTTEKLGFTGREEGIASQAVVLLQKK

Sequences:

>Translated_158_residues
MFRIGQGFDVHEFAEGRPLIIGGITIPHEKGLIGHSDADVLLHTIADACLGAIAAGDIGKHFPDTDPAFKDADSAVLLQK
VWEFVREQGYELGNLDCTIIAQKPKMAPHIESMRKRISELLETSIDNINVKATTTEKLGFTGREEGIASQAVVLLQKK
>Mature_158_residues
MFRIGQGFDVHEFAEGRPLIIGGITIPHEKGLIGHSDADVLLHTIADACLGAIAAGDIGKHFPDTDPAFKDADSAVLLQK
VWEFVREQGYELGNLDCTIIAQKPKMAPHIESMRKRISELLETSIDNINVKATTTEKLGFTGREEGIASQAVVLLQKK

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=156, Percent_Identity=64.1025641025641, Blast_Score=188, Evalue=2e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_BACAA (C3P9N2)

Other databases:

- EMBL:   CP001598
- RefSeq:   YP_002864738.1
- ProteinModelPortal:   C3P9N2
- SMR:   C3P9N2
- EnsemblBacteria:   EBBACT00000129402
- GeneID:   7848920
- GenomeReviews:   CP001598_GR
- KEGG:   bai:BAA_0102
- GeneTree:   EBGT00050000002053
- ProtClustDB:   PRK00084
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 17219; Mature: 17219

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRIGQGFDVHEFAEGRPLIIGGITIPHEKGLIGHSDADVLLHTIADACLGAIAAGDIGK
CCCCCCCCCHHHHCCCCCEEEECEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
HFPDTDPAFKDADSAVLLQKVWEFVREQGYELGNLDCTIIAQKPKMAPHIESMRKRISEL
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHH
LETSIDNINVKATTTEKLGFTGREEGIASQAVVLLQKK
HHHHHCCCEEEEECHHHCCCCCCCCCHHHHEEEEEECC
>Mature Secondary Structure
MFRIGQGFDVHEFAEGRPLIIGGITIPHEKGLIGHSDADVLLHTIADACLGAIAAGDIGK
CCCCCCCCCHHHHCCCCCEEEECEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
HFPDTDPAFKDADSAVLLQKVWEFVREQGYELGNLDCTIIAQKPKMAPHIESMRKRISEL
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHH
LETSIDNINVKATTTEKLGFTGREEGIASQAVVLLQKK
HHHHHCCCEEEEECHHHCCCCCCCCCHHHHEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA