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The map label for this gene is tilS

Identifier: 49183097

GI number: 49183097

Start: 67549

End: 68979

Strand: Direct

Name: tilS

Synonym: BAS0062

Alternate gene names: 49183097

Gene position: 67549-68979 (Clockwise)

Preceding gene: 49183096

Following gene: 49183098

Centisome position: 1.29

GC content: 36.48

Gene sequence:

>1431_bases
TTGAAAGATACATTTGTTGAAAAGGTAGATGACTTTGTAAGGCAACATGATGTATTAAAGGAACGTTCAACAATTGTTGT
AGGGGTTTCTGGTGGTCCTGACTCTTTGGCTCTTCTATATTATTTATTAGAAAAAAGAGCAGCAAAACAGTTTGAAATTG
TAGTGGCGCATGTGGATCATATGTTTAGAGGTGATGAATCTCATGAGGACCTACAGTTTGTGCAGGACCTTTGTAAAGGA
CTGGGAGTTATTTGCGAAACGATAAGGATTAATGTGTCGCAATATCAAAAGCAATACGGGATGAATGCACAGGTTGCTGC
TAGAGAATGCAGATATGCATTTTTGGAAAGAATAATGAAGAAATATGATGCGAGATATGTAGCCCTTGGACATCATGGTG
ATGATCAAGTAGAGACGATTTTAATGCGTCTTGTACGAGGGAGTACTCCGAAAGGATATGCAGGAATTGCAGTGAAGCGT
CCTTTTCATAATGGGTATTTAATTAGGCCGTTACTTGGAGTAACGAAGGAAGAAATTGTTAATTACTGTAATGAGCTAAA
AATAATTCCTCGTATAGATCCAAGTAACAAAAAGGAAGTATATACAAGGAATCGATTACGTAAATATGTTCTTCCTCATT
TGAAAGAAGAAAACCCACAAGTGCATGAGAAATTCCAAAAATTTAGCATGCAGATGCAAGAGGATGAGGCGTATTTGCAG
GAATTAGCTTTTGAGAAAATGAATAAAGTAATTACAAAAAAAAGTGATAAACAAATTAGCTTATCAATTCCTACCTTTGA
ATCCATGTCTATGCCTTTACAAAGAAGAGGGATTCAACTAATATTAAACTATCTTTATGAATATAAGATTCCATCTTCTC
TTTCCTCTATACATATTGACAAGGTGATTGAGTTTTTTAAGCGGACACAACCTTCAGGTTCACTTGATTTCCCAGGTGAT
TTGAAAATTGTTCGCGCGTACGAGGAGTGTAGCTTTGGATTTAAACAAGAAATTGTTTCCCCTTTCTTACAAGACTTATC
AGTGCCCGGGACAATTACGCTATCGAACGGGGATAAACTTGTAACAGAGGTGAGCGAAGATATACCAAGTGACATGAATG
AAACAGTATTTGTTGCTAAGTATAATGATATATCATATCCAATTCGTATTCGGTCTAGAGAAAATGGAGATCGCATGTCA
ATACAAGGTATGAATGGCACGAAAAAGATAAAAGCTATTTTTATCGAAGCAAAAGTACCAAGAGAAAAAAGAGAAGAATG
GCCGGTCGTTTGTGATGCAAGCGGGAATATTATTTGGTTACCCTTGTTGAAGCGATCTGCATTTGCTATCTCGAAAGAGA
CAGCAAAGAAGGATAAATATATGATTATTCACTACAAAAGCAAGGAGTCTTCCGGGAGGATAATGAAATGA

Upstream 100 bases:

>100_bases
TTTGTATATCTTTTTTTTGCACAATGTTTGTAAAAGTTGTACCATTATAAACAAGGTAACTAACGTTGCTGTTCTTGTTT
AATGAAAGGTGATTACGAAG

Downstream 100 bases:

>100_bases
TGAATCAAGATATCGAAAAAGTATTAATTTCTGAAGAGCAAATACAAGAAAAGGTGCTCGAATTAGGTGCGATTATTGCA
GAGGATTATAAAAACACAGT

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MKDTFVEKVDDFVRQHDVLKERSTIVVGVSGGPDSLALLYYLLEKRAAKQFEIVVAHVDHMFRGDESHEDLQFVQDLCKG
LGVICETIRINVSQYQKQYGMNAQVAARECRYAFLERIMKKYDARYVALGHHGDDQVETILMRLVRGSTPKGYAGIAVKR
PFHNGYLIRPLLGVTKEEIVNYCNELKIIPRIDPSNKKEVYTRNRLRKYVLPHLKEENPQVHEKFQKFSMQMQEDEAYLQ
ELAFEKMNKVITKKSDKQISLSIPTFESMSMPLQRRGIQLILNYLYEYKIPSSLSSIHIDKVIEFFKRTQPSGSLDFPGD
LKIVRAYEECSFGFKQEIVSPFLQDLSVPGTITLSNGDKLVTEVSEDIPSDMNETVFVAKYNDISYPIRIRSRENGDRMS
IQGMNGTKKIKAIFIEAKVPREKREEWPVVCDASGNIIWLPLLKRSAFAISKETAKKDKYMIIHYKSKESSGRIMK

Sequences:

>Translated_476_residues
MKDTFVEKVDDFVRQHDVLKERSTIVVGVSGGPDSLALLYYLLEKRAAKQFEIVVAHVDHMFRGDESHEDLQFVQDLCKG
LGVICETIRINVSQYQKQYGMNAQVAARECRYAFLERIMKKYDARYVALGHHGDDQVETILMRLVRGSTPKGYAGIAVKR
PFHNGYLIRPLLGVTKEEIVNYCNELKIIPRIDPSNKKEVYTRNRLRKYVLPHLKEENPQVHEKFQKFSMQMQEDEAYLQ
ELAFEKMNKVITKKSDKQISLSIPTFESMSMPLQRRGIQLILNYLYEYKIPSSLSSIHIDKVIEFFKRTQPSGSLDFPGD
LKIVRAYEECSFGFKQEIVSPFLQDLSVPGTITLSNGDKLVTEVSEDIPSDMNETVFVAKYNDISYPIRIRSRENGDRMS
IQGMNGTKKIKAIFIEAKVPREKREEWPVVCDASGNIIWLPLLKRSAFAISKETAKKDKYMIIHYKSKESSGRIMK
>Mature_476_residues
MKDTFVEKVDDFVRQHDVLKERSTIVVGVSGGPDSLALLYYLLEKRAAKQFEIVVAHVDHMFRGDESHEDLQFVQDLCKG
LGVICETIRINVSQYQKQYGMNAQVAARECRYAFLERIMKKYDARYVALGHHGDDQVETILMRLVRGSTPKGYAGIAVKR
PFHNGYLIRPLLGVTKEEIVNYCNELKIIPRIDPSNKKEVYTRNRLRKYVLPHLKEENPQVHEKFQKFSMQMQEDEAYLQ
ELAFEKMNKVITKKSDKQISLSIPTFESMSMPLQRRGIQLILNYLYEYKIPSSLSSIHIDKVIEFFKRTQPSGSLDFPGD
LKIVRAYEECSFGFKQEIVSPFLQDLSVPGTITLSNGDKLVTEVSEDIPSDMNETVFVAKYNDISYPIRIRSRENGDRMS
IQGMNGTKKIKAIFIEAKVPREKREEWPVVCDASGNIIWLPLLKRSAFAISKETAKKDKYMIIHYKSKESSGRIMK

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family

Homologues:

Organism=Escherichia coli, GI1786386, Length=421, Percent_Identity=27.3159144893112, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TILS_BACAN (Q81VX7)

Other databases:

- EMBL:   AE016879
- EMBL:   AE017334
- EMBL:   AE017225
- RefSeq:   NP_842631.1
- RefSeq:   YP_016665.1
- RefSeq:   YP_026349.1
- ProteinModelPortal:   Q81VX7
- SMR:   Q81VX7
- EnsemblBacteria:   EBBACT00000008642
- EnsemblBacteria:   EBBACT00000015007
- EnsemblBacteria:   EBBACT00000022501
- GeneID:   1086418
- GeneID:   2814409
- GeneID:   2851687
- GenomeReviews:   AE016879_GR
- GenomeReviews:   AE017225_GR
- GenomeReviews:   AE017334_GR
- KEGG:   ban:BA_0062
- KEGG:   bar:GBAA_0062
- KEGG:   bat:BAS0062
- TIGR:   BA_0062
- TIGR:   GBAA_0062
- GeneTree:   EBGT00050000002021
- HOGENOM:   HBG668702
- OMA:   NDISYPL
- ProtClustDB:   CLSK886565
- BioCyc:   BANT260799:BAS0062-MONOMER
- BioCyc:   BANT261594:GBAA0062-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_01161
- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11807:SF2
- SMART:   SM00977
- TIGRFAMs:   TIGR02433
- TIGRFAMs:   TIGR02432

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946; PF11734 TilS_C; SSF56037 B3_4

EC number: NA

Molecular weight: Translated: 54892; Mature: 54892

Theoretical pI: Translated: 9.28; Mature: 9.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDTFVEKVDDFVRQHDVLKERSTIVVGVSGGPDSLALLYYLLEKRAAKQFEIVVAHVDH
CCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
MFRGDESHEDLQFVQDLCKGLGVICETIRINVSQYQKQYGMNAQVAARECRYAFLERIMK
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
KYDARYVALGHHGDDQVETILMRLVRGSTPKGYAGIAVKRPFHNGYLIRPLLGVTKEEIV
HHCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCEEEHHHHCCCHHHHH
NYCNELKIIPRIDPSNKKEVYTRNRLRKYVLPHLKEENPQVHEKFQKFSMQMQEDEAYLQ
HHHHHCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ELAFEKMNKVITKKSDKQISLSIPTFESMSMPLQRRGIQLILNYLYEYKIPSSLSSIHID
HHHHHHHHHHHHCCCCCEEEEECCCHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHH
KVIEFFKRTQPSGSLDFPGDLKIVRAYEECSFGFKQEIVSPFLQDLSVPGTITLSNGDKL
HHHHHHHHCCCCCCCCCCCCCCEEEEHHHHCCCHHHHHHHHHHHHCCCCCEEEECCCCHH
VTEVSEDIPSDMNETVFVAKYNDISYPIRIRSRENGDRMSIQGMNGTKKIKAIFIEAKVP
HHHHHHHCCCCCCCEEEEEEECCCCCCEEEECCCCCCEEEEECCCCCCEEEEEEEEECCC
REKREEWPVVCDASGNIIWLPLLKRSAFAISKETAKKDKYMIIHYKSKESSGRIMK
HHHHCCCCEEEECCCCEEEEEEHHCHHHHHHHHHCCCCCEEEEEEECCCCCCCCCC
>Mature Secondary Structure
MKDTFVEKVDDFVRQHDVLKERSTIVVGVSGGPDSLALLYYLLEKRAAKQFEIVVAHVDH
CCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
MFRGDESHEDLQFVQDLCKGLGVICETIRINVSQYQKQYGMNAQVAARECRYAFLERIMK
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
KYDARYVALGHHGDDQVETILMRLVRGSTPKGYAGIAVKRPFHNGYLIRPLLGVTKEEIV
HHCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCEEEHHHHCCCHHHHH
NYCNELKIIPRIDPSNKKEVYTRNRLRKYVLPHLKEENPQVHEKFQKFSMQMQEDEAYLQ
HHHHHCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ELAFEKMNKVITKKSDKQISLSIPTFESMSMPLQRRGIQLILNYLYEYKIPSSLSSIHID
HHHHHHHHHHHHCCCCCEEEEECCCHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHH
KVIEFFKRTQPSGSLDFPGDLKIVRAYEECSFGFKQEIVSPFLQDLSVPGTITLSNGDKL
HHHHHHHHCCCCCCCCCCCCCCEEEEHHHHCCCHHHHHHHHHHHHCCCCCEEEECCCCHH
VTEVSEDIPSDMNETVFVAKYNDISYPIRIRSRENGDRMSIQGMNGTKKIKAIFIEAKVP
HHHHHHHCCCCCCCEEEEEEECCCCCCEEEECCCCCCEEEEECCCCCCEEEEEEEEECCC
REKREEWPVVCDASGNIIWLPLLKRSAFAISKETAKKDKYMIIHYKSKESSGRIMK
HHHHCCCCEEEECCCCEEEEEEHHCHHHHHHHHHCCCCCEEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12721629