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The map label for this gene is metG

Identifier: 49183072

GI number: 49183072

Start: 41599

End: 43581

Strand: Direct

Name: metG

Synonym: BAS0037

Alternate gene names: 49183072

Gene position: 41599-43581 (Clockwise)

Preceding gene: 49183070

Following gene: 49183073

Centisome position: 0.8

GC content: 36.01

Gene sequence:

>1983_bases
ATGACAGAGGAAAACAAATCCTTTTATATTACTACCCCAATTTATTATCCAAGTGGAAAATTACACATTGGACATGCTTA
TACAACAGTAGCAGGAGATGCGATGGCACGATATAAGTGCATGCAAGGATATAATGTTCATTATTTAACAGGAACTGATG
AGCATGGACAGAAGATCCAAAAGAAAGCAGAAGAATTAAACGTTACACCACAAGCATATGTGGATAACATCGTTGCAGGA
ATTAAAGAACTTTGGGAAAAGATGGATATCTCTTATGATGATTTTATTCGTACAACGGAAGATCGTCATAAAGATGTTGT
TGAAAAAATCTTCAAGCAACTAGTAGATCAAGGTGATATTTATCTTGATGAATATGAAGGTTGGTATTCTGTACAAGATG
AGACATTCTATACGGAGCATCAATTAGTAGATCCAATTATGGAAGGTGACAAAGTAGTTGGAGGAAAAAGTCCAGATAGT
GGTCATGATGTAGAACTTGTTCGTGAAGAATCGTATTTCTTTAGAATGGGTAAATATGTCGACAGACTATTAAAATTCTA
TGAAGATAATCCACATTTCATTCAGCCAGAGTCTCGTAAGAATGAAATGATTAATAACTTCATTAAACCAGGCTTAGAAG
ATTTAGCAGTTTCTCGTACTTCATTTGACTGGGGAGTTCGTGTTCCAGGTAATCCGAAACACGTTATTTACGTATGGGTT
GACGCACTATCTAACTACATTACAGCATTAGGCTATGGAACAGAAAATGAAGAGATGTATAAGAAGTTCTGGCCGGCAGA
TGTGCATTTAGTTGGAAAAGAAATCGTTCGTTTCCATACAATTTATTGGCCAATCATTTTAATGGCATTAGATTTACCTC
TTCCGAAAAAAGTCTTTGCTCACGGTTGGATTTTAATGAAGGATGGAAAGATGAGTAAGTCAAAAGGAAATGTAGTAGAT
CCAGTTATATTAATCGATCGTTACGGATTAGATGCATTACGTTATTACTTACTTCGTGAAGTTCCATTCGGATCTGATGG
CGTATTCACACCAGAAGGATTTGTGGAGCGTATTAATTTCGATTTAGCAAATGACTTAGGTAACTTATTAAATCGTACAG
TAGCTATGATTGATAAGTACTTTAACGGAGAAATCCCTGCATTTAAAGCAAATGTAACGGAATTTGATGAAACGTTAGTA
ACATTTGCGAAAGATACATTGAAAAAAGTTGAAGAAGCAATGGAGAATATGGAATTCTCTGTAGCTCTAAGCTCAATTTG
GCAATTGGTTAGCCGTACGAATAAATATATTGATGAAACACAACCATGGGTATTAGCGAAAGATGAGGATGATCGTGAAA
AGTTGGCGTCTGTAATGGCTCATTTAGCAGAAGTACTTCGTCAAACGGGTATTATGCTTATGCCATTCTTAACAGTAGCA
CCAAGCAAAATGTTTGCTCAGCTTGGCCTTACTGATGAAGCACATACATCTTGGGGAAGCCTATCTACAATCGGCTGTAT
TCCAGCTGGAACAAAAGTAGAAAAAGGACAACCAATTTTCCCTCGTTTAGAAATGGACGTGGAAGTGGCGTACATTAAAG
AACAAATGAAAGCCTCTGCTCCTAAAGTAGAAGAGAAAAAAGAAGAAGAACCGAAGGCAGAAGAAATTACAATTGATGAT
TTCTTTAAAGTAGAATTGCGTGTAGCTGAAGTACTATCTGCTGAACCTGTAAAAAAGGCAGATAAGTTGTTGAAAATTCA
ACTAGACTTAGGTACAGAAAAGCGTCAAGTTGTTTCTGGAATTGCAAAATTCTATTCGCCAGAAGACCTAAAAGGTAAAA
AGGTTATTTGTGTAACAAATTTAAAACCTGTAAAATTACGCGGTGAATTATCGCAAGGTATGATTTTAGCGGGTGAAGAG
AATGGTGTATTGTCATTAGCATCTATTGACCAAAATATACCAAATGGTACAAAAATCAAGTAA

Upstream 100 bases:

>100_bases
GAGGGAACAAAACCCCGACAGATTAAAGTTTCACTTTATATATAAGTTTTTGATATACTATTTATAAATACATATGAGGT
TATTCGGGAGGTTCAAAACA

Downstream 100 bases:

>100_bases
TTAAGACAAGAAAAGAGATGTTTCACGTGTAACATGTGTGAAGCGTCTTTTTTCTTTTTACACTCTCATTTTTGCATTAA
GATTGTAGTATTGTTATCGT

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase 1; MetRS 1

Number of amino acids: Translated: 660; Mature: 659

Protein sequence:

>660_residues
MTEENKSFYITTPIYYPSGKLHIGHAYTTVAGDAMARYKCMQGYNVHYLTGTDEHGQKIQKKAEELNVTPQAYVDNIVAG
IKELWEKMDISYDDFIRTTEDRHKDVVEKIFKQLVDQGDIYLDEYEGWYSVQDETFYTEHQLVDPIMEGDKVVGGKSPDS
GHDVELVREESYFFRMGKYVDRLLKFYEDNPHFIQPESRKNEMINNFIKPGLEDLAVSRTSFDWGVRVPGNPKHVIYVWV
DALSNYITALGYGTENEEMYKKFWPADVHLVGKEIVRFHTIYWPIILMALDLPLPKKVFAHGWILMKDGKMSKSKGNVVD
PVILIDRYGLDALRYYLLREVPFGSDGVFTPEGFVERINFDLANDLGNLLNRTVAMIDKYFNGEIPAFKANVTEFDETLV
TFAKDTLKKVEEAMENMEFSVALSSIWQLVSRTNKYIDETQPWVLAKDEDDREKLASVMAHLAEVLRQTGIMLMPFLTVA
PSKMFAQLGLTDEAHTSWGSLSTIGCIPAGTKVEKGQPIFPRLEMDVEVAYIKEQMKASAPKVEEKKEEEPKAEEITIDD
FFKVELRVAEVLSAEPVKKADKLLKIQLDLGTEKRQVVSGIAKFYSPEDLKGKKVICVTNLKPVKLRGELSQGMILAGEE
NGVLSLASIDQNIPNGTKIK

Sequences:

>Translated_660_residues
MTEENKSFYITTPIYYPSGKLHIGHAYTTVAGDAMARYKCMQGYNVHYLTGTDEHGQKIQKKAEELNVTPQAYVDNIVAG
IKELWEKMDISYDDFIRTTEDRHKDVVEKIFKQLVDQGDIYLDEYEGWYSVQDETFYTEHQLVDPIMEGDKVVGGKSPDS
GHDVELVREESYFFRMGKYVDRLLKFYEDNPHFIQPESRKNEMINNFIKPGLEDLAVSRTSFDWGVRVPGNPKHVIYVWV
DALSNYITALGYGTENEEMYKKFWPADVHLVGKEIVRFHTIYWPIILMALDLPLPKKVFAHGWILMKDGKMSKSKGNVVD
PVILIDRYGLDALRYYLLREVPFGSDGVFTPEGFVERINFDLANDLGNLLNRTVAMIDKYFNGEIPAFKANVTEFDETLV
TFAKDTLKKVEEAMENMEFSVALSSIWQLVSRTNKYIDETQPWVLAKDEDDREKLASVMAHLAEVLRQTGIMLMPFLTVA
PSKMFAQLGLTDEAHTSWGSLSTIGCIPAGTKVEKGQPIFPRLEMDVEVAYIKEQMKASAPKVEEKKEEEPKAEEITIDD
FFKVELRVAEVLSAEPVKKADKLLKIQLDLGTEKRQVVSGIAKFYSPEDLKGKKVICVTNLKPVKLRGELSQGMILAGEE
NGVLSLASIDQNIPNGTKIK
>Mature_659_residues
TEENKSFYITTPIYYPSGKLHIGHAYTTVAGDAMARYKCMQGYNVHYLTGTDEHGQKIQKKAEELNVTPQAYVDNIVAGI
KELWEKMDISYDDFIRTTEDRHKDVVEKIFKQLVDQGDIYLDEYEGWYSVQDETFYTEHQLVDPIMEGDKVVGGKSPDSG
HDVELVREESYFFRMGKYVDRLLKFYEDNPHFIQPESRKNEMINNFIKPGLEDLAVSRTSFDWGVRVPGNPKHVIYVWVD
ALSNYITALGYGTENEEMYKKFWPADVHLVGKEIVRFHTIYWPIILMALDLPLPKKVFAHGWILMKDGKMSKSKGNVVDP
VILIDRYGLDALRYYLLREVPFGSDGVFTPEGFVERINFDLANDLGNLLNRTVAMIDKYFNGEIPAFKANVTEFDETLVT
FAKDTLKKVEEAMENMEFSVALSSIWQLVSRTNKYIDETQPWVLAKDEDDREKLASVMAHLAEVLRQTGIMLMPFLTVAP
SKMFAQLGLTDEAHTSWGSLSTIGCIPAGTKVEKGQPIFPRLEMDVEVAYIKEQMKASAPKVEEKKEEEPKAEEITIDDF
FKVELRVAEVLSAEPVKKADKLLKIQLDLGTEKRQVVSGIAKFYSPEDLKGKKVICVTNLKPVKLRGELSQGMILAGEEN
GVLSLASIDQNIPNGTKIK

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

Organism=Homo sapiens, GI24308436, Length=505, Percent_Identity=34.8514851485148, Blast_Score=302, Evalue=8e-82,
Organism=Homo sapiens, GI14043022, Length=656, Percent_Identity=25.7621951219512, Blast_Score=177, Evalue=3e-44,
Organism=Escherichia coli, GI1788432, Length=704, Percent_Identity=29.5454545454545, Blast_Score=285, Evalue=8e-78,
Organism=Escherichia coli, GI1789455, Length=108, Percent_Identity=34.2592592592593, Blast_Score=72, Evalue=2e-13,
Organism=Escherichia coli, GI1786861, Length=208, Percent_Identity=25.4807692307692, Blast_Score=64, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI212645096, Length=491, Percent_Identity=32.3828920570265, Blast_Score=271, Evalue=8e-73,
Organism=Caenorhabditis elegans, GI71992781, Length=383, Percent_Identity=31.8537859007833, Blast_Score=196, Evalue=4e-50,
Organism=Caenorhabditis elegans, GI17540794, Length=593, Percent_Identity=25.1264755480607, Blast_Score=164, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6321610, Length=491, Percent_Identity=31.9755600814664, Blast_Score=259, Evalue=8e-70,
Organism=Saccharomyces cerevisiae, GI6321704, Length=489, Percent_Identity=24.7443762781186, Blast_Score=121, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6323414, Length=231, Percent_Identity=28.1385281385281, Blast_Score=70, Evalue=9e-13,
Organism=Drosophila melanogaster, GI21358483, Length=574, Percent_Identity=32.7526132404181, Blast_Score=289, Evalue=6e-78,
Organism=Drosophila melanogaster, GI24655311, Length=562, Percent_Identity=26.8683274021352, Blast_Score=176, Evalue=6e-44,
Organism=Drosophila melanogaster, GI21355409, Length=184, Percent_Identity=27.7173913043478, Blast_Score=70, Evalue=6e-12,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM1_BACAN (Q81W03)

Other databases:

- EMBL:   AE016879
- EMBL:   AE017334
- EMBL:   AE017225
- RefSeq:   NP_842605.1
- RefSeq:   YP_016640.2
- RefSeq:   YP_026324.1
- ProteinModelPortal:   Q81W03
- IntAct:   Q81W03
- EnsemblBacteria:   EBBACT00000012543
- EnsemblBacteria:   EBBACT00000014151
- EnsemblBacteria:   EBBACT00000024146
- GeneID:   1084885
- GeneID:   2816080
- GeneID:   2848894
- GenomeReviews:   AE016879_GR
- GenomeReviews:   AE017225_GR
- GenomeReviews:   AE017334_GR
- KEGG:   ban:BA_0036
- KEGG:   bar:GBAA_0036
- KEGG:   bat:BAS0037
- TIGR:   BA_0036
- TIGR:   GBAA_0036
- GeneTree:   EBGT00070000031891
- HOGENOM:   HBG721667
- OMA:   APHIGHA
- ProtClustDB:   PRK12267
- BioCyc:   BANT260799:BAS0037-MONOMER
- BioCyc:   BANT261594:GBAA0036-MONOMER
- BRENDA:   6.1.1.10
- GO:   GO:0005737
- HAMAP:   MF_01228
- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398
- TIGRFAMs:   TIGR00399

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind; SSF50249 Nucleic_acid_OB; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 75126; Mature: 74995

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50886 TRBD

Important sites: BINDING 313-313

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEENKSFYITTPIYYPSGKLHIGHAYTTVAGDAMARYKCMQGYNVHYLTGTDEHGQKIQ
CCCCCCCEEEEECEEECCCCEEECCEEHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHH
KKAEELNVTPQAYVDNIVAGIKELWEKMDISYDDFIRTTEDRHKDVVEKIFKQLVDQGDI
HHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCCE
YLDEYEGWYSVQDETFYTEHQLVDPIMEGDKVVGGKSPDSGHDVELVREESYFFRMGKYV
EEECCCCEEECCCCEEEHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEHHHHHHHHHHHH
DRLLKFYEDNPHFIQPESRKNEMINNFIKPGLEDLAVSRTSFDWGVRVPGNPKHVIYVWV
HHHHHHHCCCCCEECCCHHHHHHHHHHHCCCHHHHHHCCCCCCCCEECCCCCCEEEEEEH
DALSNYITALGYGTENEEMYKKFWPADVHLVGKEIVRFHTIYWPIILMALDLPLPKKVFA
HHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
HGWILMKDGKMSKSKGNVVDPVILIDRYGLDALRYYLLREVPFGSDGVFTPEGFVERINF
CCEEEEECCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCH
DLANDLGNLLNRTVAMIDKYFNGEIPAFKANVTEFDETLVTFAKDTLKKVEEAMENMEFS
HHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
VALSSIWQLVSRTNKYIDETQPWVLAKDEDDREKLASVMAHLAEVLRQTGIMLMPFLTVA
HHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHC
PSKMFAQLGLTDEAHTSWGSLSTIGCIPAGTKVEKGQPIFPRLEMDVEVAYIKEQMKASA
HHHHHHHHCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCC
PKVEEKKEEEPKAEEITIDDFFKVELRVAEVLSAEPVKKADKLLKIQLDLGTEKRQVVSG
CCCHHHHHCCCCCCEEEHHHHHHHHHHHHHHHCCCCHHHHCCEEEEEECCCCHHHHHHHH
IAKFYSPEDLKGKKVICVTNLKPVKLRGELSQGMILAGEENGVLSLASIDQNIPNGTKIK
HHHHCCCCCCCCCEEEEEECCCCEEEECCCCCCEEEECCCCCEEEEEECCCCCCCCCCCC
>Mature Secondary Structure 
TEENKSFYITTPIYYPSGKLHIGHAYTTVAGDAMARYKCMQGYNVHYLTGTDEHGQKIQ
CCCCCCEEEEECEEECCCCEEECCEEHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHH
KKAEELNVTPQAYVDNIVAGIKELWEKMDISYDDFIRTTEDRHKDVVEKIFKQLVDQGDI
HHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCCE
YLDEYEGWYSVQDETFYTEHQLVDPIMEGDKVVGGKSPDSGHDVELVREESYFFRMGKYV
EEECCCCEEECCCCEEEHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEHHHHHHHHHHHH
DRLLKFYEDNPHFIQPESRKNEMINNFIKPGLEDLAVSRTSFDWGVRVPGNPKHVIYVWV
HHHHHHHCCCCCEECCCHHHHHHHHHHHCCCHHHHHHCCCCCCCCEECCCCCCEEEEEEH
DALSNYITALGYGTENEEMYKKFWPADVHLVGKEIVRFHTIYWPIILMALDLPLPKKVFA
HHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
HGWILMKDGKMSKSKGNVVDPVILIDRYGLDALRYYLLREVPFGSDGVFTPEGFVERINF
CCEEEEECCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCH
DLANDLGNLLNRTVAMIDKYFNGEIPAFKANVTEFDETLVTFAKDTLKKVEEAMENMEFS
HHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
VALSSIWQLVSRTNKYIDETQPWVLAKDEDDREKLASVMAHLAEVLRQTGIMLMPFLTVA
HHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHC
PSKMFAQLGLTDEAHTSWGSLSTIGCIPAGTKVEKGQPIFPRLEMDVEVAYIKEQMKASA
HHHHHHHHCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCC
PKVEEKKEEEPKAEEITIDDFFKVELRVAEVLSAEPVKKADKLLKIQLDLGTEKRQVVSG
CCCHHHHHCCCCCCEEEHHHHHHHHHHHHHHHCCCCHHHHCCEEEEEECCCCHHHHHHHH
IAKFYSPEDLKGKKVICVTNLKPVKLRGELSQGMILAGEENGVLSLASIDQNIPNGTKIK
HHHHCCCCCCCCCEEEEEECCCCEEEECCCCCCEEEECCCCCEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12721629