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The map label for this gene is phoB [H]

Identifier: 46447489

GI number: 46447489

Start: 2215139

End: 2215828

Strand: Reverse

Name: phoB [H]

Synonym: pc1855

Alternate gene names: 46447489

Gene position: 2215828-2215139 (Counterclockwise)

Preceding gene: 46447495

Following gene: 46447488

Centisome position: 91.77

GC content: 33.48

Gene sequence:

>690_bases
ATGCAAAAAACAAAAATATTGCTTATTGAAGATGAAGAGGATATAGCCTCTTTGATTAAACTTCAGGCAGAATTGGCTGG
ATATAAAGTCCATCTGGAAATGGATGGATTAAATGGTTTTTTGGCTATAGAAAGAGAAAAACCAGATGTTATTATCCTTG
ATATTATGCTACCTGGATTAAACGGACTAGATGTATGCCGTAAAATCAAAAGTAATCCTGATTTAGAAAATATTCCTATT
ATTATAATTTCAGCAAAAAGCGAAGAGCTCGATGTTGTTTTAGGACTTGAGCTAGGTGCAGATGATTATGTCGCTAAACC
ATTTTCTCTAAAGGTTTTATTTTCACGTATTAAAGCTGTTATGAGACGTGGAAAAGAAGGAGAAAAGGAAGCAAAAACAT
TAGTTTTTGGCGAATACACCATGGAAGTCGATCGGTATTTGTTGAGAAAGAAAGATAAATCTATTGCTTTAACCCTTTCT
GAATTTGGAATTTTGAGACGCTTACTTCTTAATCGTGGAAAGGTATTGACTAGAAATCAATTATTAGACGATGTACAAAA
TGACGAAGCCTTTATCATCGATCGTAACATTGATGTGCATATTGCCTCTTTGAGAAAAAAATTAGGTCCTAATTTTGATG
GTATTGAAACTGTCAGAGGGGTAGGTTATCGTTTTAAAGACGATGAATAA

Upstream 100 bases:

>100_bases
GTAGTTATCAAATGAAAATAAAAATAATTATAATAAAAAAACTTGAATATTAGAAAAATTTAAATTATAAAATACCAATT
CAGTACGCGGAGCTATTAAA

Downstream 100 bases:

>100_bases
GATAAATTAAAACTGAATTAATCCTTTGCAGGATTAATTCAGTTTTTCTCGGGTATGAGAATGTGGGTTCTAAAACTTAT
TATTTTTAGCTTCTTTTTTT

Product: putative two-component response regulator phoP

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MQKTKILLIEDEEDIASLIKLQAELAGYKVHLEMDGLNGFLAIEREKPDVIILDIMLPGLNGLDVCRKIKSNPDLENIPI
IIISAKSEELDVVLGLELGADDYVAKPFSLKVLFSRIKAVMRRGKEGEKEAKTLVFGEYTMEVDRYLLRKKDKSIALTLS
EFGILRRLLLNRGKVLTRNQLLDDVQNDEAFIIDRNIDVHIASLRKKLGPNFDGIETVRGVGYRFKDDE

Sequences:

>Translated_229_residues
MQKTKILLIEDEEDIASLIKLQAELAGYKVHLEMDGLNGFLAIEREKPDVIILDIMLPGLNGLDVCRKIKSNPDLENIPI
IIISAKSEELDVVLGLELGADDYVAKPFSLKVLFSRIKAVMRRGKEGEKEAKTLVFGEYTMEVDRYLLRKKDKSIALTLS
EFGILRRLLLNRGKVLTRNQLLDDVQNDEAFIIDRNIDVHIASLRKKLGPNFDGIETVRGVGYRFKDDE
>Mature_229_residues
MQKTKILLIEDEEDIASLIKLQAELAGYKVHLEMDGLNGFLAIEREKPDVIILDIMLPGLNGLDVCRKIKSNPDLENIPI
IIISAKSEELDVVLGLELGADDYVAKPFSLKVLFSRIKAVMRRGKEGEKEAKTLVFGEYTMEVDRYLLRKKDKSIALTLS
EFGILRRLLLNRGKVLTRNQLLDDVQNDEAFIIDRNIDVHIASLRKKLGPNFDGIETVRGVGYRFKDDE

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790860, Length=230, Percent_Identity=36.5217391304348, Blast_Score=156, Evalue=1e-39,
Organism=Escherichia coli, GI1788394, Length=228, Percent_Identity=36.8421052631579, Blast_Score=148, Evalue=4e-37,
Organism=Escherichia coli, GI1786599, Length=224, Percent_Identity=35.2678571428571, Blast_Score=136, Evalue=1e-33,
Organism=Escherichia coli, GI1789809, Length=240, Percent_Identity=33.3333333333333, Blast_Score=135, Evalue=2e-33,
Organism=Escherichia coli, GI2367329, Length=229, Percent_Identity=35.8078602620087, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1786911, Length=228, Percent_Identity=33.7719298245614, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1786784, Length=222, Percent_Identity=37.8378378378378, Blast_Score=118, Evalue=3e-28,
Organism=Escherichia coli, GI1789402, Length=219, Percent_Identity=32.8767123287671, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI1787375, Length=224, Percent_Identity=31.6964285714286, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI1790552, Length=226, Percent_Identity=31.858407079646, Blast_Score=108, Evalue=2e-25,
Organism=Escherichia coli, GI1790863, Length=238, Percent_Identity=30.672268907563, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI87082012, Length=224, Percent_Identity=32.1428571428571, Blast_Score=101, Evalue=4e-23,
Organism=Escherichia coli, GI145693140, Length=233, Percent_Identity=30.4721030042918, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787229, Length=230, Percent_Identity=30.4347826086957, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1788550, Length=106, Percent_Identity=29.2452830188679, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 25953; Mature: 25953

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKTKILLIEDEEDIASLIKLQAELAGYKVHLEMDGLNGFLAIEREKPDVIILDIMLPGL
CCCEEEEEEECCHHHHHHHHHHHHHCCEEEEEEECCCCEEEEEEECCCCEEEEEEEECCC
NGLDVCRKIKSNPDLENIPIIIISAKSEELDVVLGLELGADDYVAKPFSLKVLFSRIKAV
CHHHHHHHHHCCCCCCCCCEEEEECCCCCEEEEEEEEECCCCHHCCCHHHHHHHHHHHHH
MRRGKEGEKEAKTLVFGEYTMEVDRYLLRKKDKSIALTLSEFGILRRLLLNRGKVLTRNQ
HHCCCCCCCHHHEEEEEHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHCCCCEEEHHH
LLDDVQNDEAFIIDRNIDVHIASLRKKLGPNFDGIETVRGVGYRFKDDE
HHHHCCCCCEEEEECCCCEEHHHHHHHHCCCCCCHHHHHHCCEEECCCC
>Mature Secondary Structure
MQKTKILLIEDEEDIASLIKLQAELAGYKVHLEMDGLNGFLAIEREKPDVIILDIMLPGL
CCCEEEEEEECCHHHHHHHHHHHHHCCEEEEEEECCCCEEEEEEECCCCEEEEEEEECCC
NGLDVCRKIKSNPDLENIPIIIISAKSEELDVVLGLELGADDYVAKPFSLKVLFSRIKAV
CHHHHHHHHHCCCCCCCCCEEEEECCCCCEEEEEEEEECCCCHHCCCHHHHHHHHHHHHH
MRRGKEGEKEAKTLVFGEYTMEVDRYLLRKKDKSIALTLSEFGILRRLLLNRGKVLTRNQ
HHCCCCCCCHHHEEEEEHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHCCCCEEEHHH
LLDDVQNDEAFIIDRNIDVHIASLRKKLGPNFDGIETVRGVGYRFKDDE
HHHHCCCCCEEEEECCCCEEHHHHHHHHCCCCCCHHHHHHCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]