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The map label for this gene is rlmL [H]

Identifier: 46447427

GI number: 46447427

Start: 2147469

End: 2148332

Strand: Reverse

Name: rlmL [H]

Synonym: pc1793

Alternate gene names: 46447427

Gene position: 2148332-2147469 (Counterclockwise)

Preceding gene: 46447429

Following gene: 46447426

Centisome position: 88.98

GC content: 39.12

Gene sequence:

>864_bases
ATGACACTACCTTTTCAATCCTATGCTTTAATTGACAGTGGAAATGGTCGTAAACTTGAGCAGTTTGGTCCCTATGTACT
TTCCCGTCCTTGTTCGCAAGCTGTTTGGAAACCTCAACTCTCATTGGCAAAATGGAATGAAGCACAGGCAAGTTTTTCTC
GAGAATTGGAAAATAAATGGACGAATCTGTCTAATATCCCTGAGGTATGGCACATTGAAGTTGCTGGTGTCGTGTTTAAA
ATTTCAGCGACAGATTTTGGACATTTAGGAATTTTCCCTGAACAAAAGTCTTTTTGGGATTGGATTCAAAAAATTTTAAG
GCCTTACGCTTCCAAAAAAGAAAAACCTCGTATTTTAAATCTTTTTGCCTATTCGGGAGGGTCTACATTAGCAGCAGCAA
AAGCAGGAGCTGCGGTATGTCATTTAGATGCTTCTAAAGGAATGGTGACCTGGGCAAGAGAAAATGCAGCTTTAAATCAT
CTCGAGCATGCACCTATTCGATGGATTGTGGAAGATGTCAAAAAATTTTTAGCAAGAGAATTAAAACGAGGATCTCGTTA
TGAAGGCATTATTTTAGATCCACCTAGTTTCGGGAGAGGTTCTAAAGGAGAACTATTTAAAATTGAAGATGAAGTTACTC
AACTTCTCCAAAGTTGTCGTGATTTACTCAGTGAAAGACCTCTTTTTGTGATGTTTTCCTGCCATACTCCAGGATTTTCT
CCTGTCGTTATGCATCATCTATTAAGCCAGGCTATGCAAGGAGTGAAAGGAAAGATCGAATTTGGAGAAATGTTATTAGA
GGGACCTGATTCTTTGAAGCTTCCAAGCGGCACTTATGCGAAATGGGTGAGAGAAAATGATTGA

Upstream 100 bases:

>100_bases
GAAGCAACACATAAATCACGTTGTTGCTTTCTTCTATGTATGTTATGCTTGTAAAAAAAACAATTGGGAAAATACATTTA
CCCTATTTTCAAGAGTAATA

Downstream 100 bases:

>100_bases
GCTGACGAGTGTGCAAAATCCTAAGGTTAAGCAAATATTACATTTACGTGATCGACCCGAAAGAGATATTTCGCAAACTT
TTTTAATTGAGGGTTATAGA

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL [H]

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MTLPFQSYALIDSGNGRKLEQFGPYVLSRPCSQAVWKPQLSLAKWNEAQASFSRELENKWTNLSNIPEVWHIEVAGVVFK
ISATDFGHLGIFPEQKSFWDWIQKILRPYASKKEKPRILNLFAYSGGSTLAAAKAGAAVCHLDASKGMVTWARENAALNH
LEHAPIRWIVEDVKKFLARELKRGSRYEGIILDPPSFGRGSKGELFKIEDEVTQLLQSCRDLLSERPLFVMFSCHTPGFS
PVVMHHLLSQAMQGVKGKIEFGEMLLEGPDSLKLPSGTYAKWVREND

Sequences:

>Translated_287_residues
MTLPFQSYALIDSGNGRKLEQFGPYVLSRPCSQAVWKPQLSLAKWNEAQASFSRELENKWTNLSNIPEVWHIEVAGVVFK
ISATDFGHLGIFPEQKSFWDWIQKILRPYASKKEKPRILNLFAYSGGSTLAAAKAGAAVCHLDASKGMVTWARENAALNH
LEHAPIRWIVEDVKKFLARELKRGSRYEGIILDPPSFGRGSKGELFKIEDEVTQLLQSCRDLLSERPLFVMFSCHTPGFS
PVVMHHLLSQAMQGVKGKIEFGEMLLEGPDSLKLPSGTYAKWVREND
>Mature_286_residues
TLPFQSYALIDSGNGRKLEQFGPYVLSRPCSQAVWKPQLSLAKWNEAQASFSRELENKWTNLSNIPEVWHIEVAGVVFKI
SATDFGHLGIFPEQKSFWDWIQKILRPYASKKEKPRILNLFAYSGGSTLAAAKAGAAVCHLDASKGMVTWARENAALNHL
EHAPIRWIVEDVKKFLARELKRGSRYEGIILDPPSFGRGSKGELFKIEDEVTQLLQSCRDLLSERPLFVMFSCHTPGFSP
VVMHHLLSQAMQGVKGKIEFGEMLLEGPDSLKLPSGTYAKWVREND

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=182, Percent_Identity=26.9230769230769, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020 [H]

EC number: =2.1.1.173 [H]

Molecular weight: Translated: 32319; Mature: 32187

Theoretical pI: Translated: 8.87; Mature: 8.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLPFQSYALIDSGNGRKLEQFGPYVLSRPCSQAVWKPQLSLAKWNEAQASFSRELENKW
CCCCCCCEEEEECCCCCCHHHHCCHHHCCCHHHHHCCCCCHHHHCCHHHHHHHHHHHHHH
TNLSNIPEVWHIEVAGVVFKISATDFGHLGIFPEQKSFWDWIQKILRPYASKKEKPRILN
CCCCCCCCEEEEEEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEE
LFAYSGGSTLAAAKAGAAVCHLDASKGMVTWARENAALNHLEHAPIRWIVEDVKKFLARE
EEEECCCCEEHHHHCCCEEEEEECCCCEEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHH
LKRGSRYEGIILDPPSFGRGSKGELFKIEDEVTQLLQSCRDLLSERPLFVMFSCHTPGFS
HHCCCCCCCEEECCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCC
PVVMHHLLSQAMQGVKGKIEFGEMLLEGPDSLKLPSGTYAKWVREND
HHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCHHHHHCCCCC
>Mature Secondary Structure 
TLPFQSYALIDSGNGRKLEQFGPYVLSRPCSQAVWKPQLSLAKWNEAQASFSRELENKW
CCCCCCEEEEECCCCCCHHHHCCHHHCCCHHHHHCCCCCHHHHCCHHHHHHHHHHHHHH
TNLSNIPEVWHIEVAGVVFKISATDFGHLGIFPEQKSFWDWIQKILRPYASKKEKPRILN
CCCCCCCCEEEEEEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEE
LFAYSGGSTLAAAKAGAAVCHLDASKGMVTWARENAALNHLEHAPIRWIVEDVKKFLARE
EEEECCCCEEHHHHCCCEEEEEECCCCEEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHH
LKRGSRYEGIILDPPSFGRGSKGELFKIEDEVTQLLQSCRDLLSERPLFVMFSCHTPGFS
HHCCCCCCCEEECCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCC
PVVMHHLLSQAMQGVKGKIEFGEMLLEGPDSLKLPSGTYAKWVREND
HHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA