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The map label for this gene is mdh

Identifier: 46447406

GI number: 46447406

Start: 2124504

End: 2125496

Strand: Reverse

Name: mdh

Synonym: pc1772

Alternate gene names: 46447406

Gene position: 2125496-2124504 (Counterclockwise)

Preceding gene: 46447408

Following gene: 46447405

Centisome position: 88.03

GC content: 38.27

Gene sequence:

>993_bases
ATGTCTCAACCAATCAAAATTGCAATTTCGGGAGGAGCGGGGCAAATTGCTTATAGCTTATTATTTCGCTTAGCGAGCGG
AGAATTGTTTGGGCCTAATCAATTGATTGAATTACAGGTTTTAGAAGTTCCAAATGCTTTGTCAGCTTTAGAAGGCGTAA
AAATGGAGATAGAAGATTGTGCATTTCCGCTTCTATCTTCCATTAAGATTTGCAGCGATCCGTATCAAGCATTTGAGGAC
ATTGATTACGCTCTTTTAATTGGTGCAAAGTCACGCGGTCCTGGAATGGAGCGAAGAGATTTATTACAGGAAAATAGTAA
AATTTTTGTTAATCAGGGACAGGCTTTAAATGCTGTGGCAAAGTCTTCAGCTAAGATATTTGTTGTCGGGAATCCTTGTA
ATACGAATTGTTTAATTGCATTAAACAACGCCCCCAGCTTAAAAAGAGAAAATTTTTATGCTATGACTCGTTTAGATCAA
AATCGAGCTACGTTTTTTCTTTCCCAAAAAAGTCAAGTTTCAACAAAGGATGTCTCTTGTGTAACGATTTGGGGAAACCA
CTCTGCGACGCAGGTTCCTGATTTTGTGAATGCAAAAATTAGTCAGAAACCGGTTGAAACTATAATTCCCGACCGTCAAT
GGTTAGAAAAAGATTTTATAGAGTCTGTTCAAAAACGAGGAGCAGCGATCATTCAAGCTAGAGGAAAATCATCTGCAGCT
TCTGCGGCCAGTGCTTTATTAGACGCCATGCGAGACCGGATTTTACCAACCCCCACCGGACAATGGTTTTCTACTGCTCT
GTTGTCAGATGGGAATCCTTATGGAATTGAAGAAGGATTAATTTTCTCTTTCCCTTGTCGCGTCAAAAAAAATGGAGAGC
TTTCTATTGTTTCAGGGCTTAAATGGGATGCATTTTTGGAGGAAAAAATTAAATTAACAGAGCAAGAATTAAAAGAAGAA
AGAGAGATGGTTAGTTCAATATTAAAAATTTGA

Upstream 100 bases:

>100_bases
TTATTTGTTAATTGTTTAACTGGATCAAAATTGAATAAATTTTTTTGCGAAATATAAAGGGGAAAACTATAGTGAGGATA
ACTACGTTCACGGAGGGAAT

Downstream 100 bases:

>100_bases
AATATTTTATTTTTTTATTTGATATAAAGATGGATTAAACCCAAGGAATCGTTATGTCTGAAGTCCTGTTCGAAATAACA
AAAGAAAGCTTAGAAACTGG

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MSQPIKIAISGGAGQIAYSLLFRLASGELFGPNQLIELQVLEVPNALSALEGVKMEIEDCAFPLLSSIKICSDPYQAFED
IDYALLIGAKSRGPGMERRDLLQENSKIFVNQGQALNAVAKSSAKIFVVGNPCNTNCLIALNNAPSLKRENFYAMTRLDQ
NRATFFLSQKSQVSTKDVSCVTIWGNHSATQVPDFVNAKISQKPVETIIPDRQWLEKDFIESVQKRGAAIIQARGKSSAA
SAASALLDAMRDRILPTPTGQWFSTALLSDGNPYGIEEGLIFSFPCRVKKNGELSIVSGLKWDAFLEEKIKLTEQELKEE
REMVSSILKI

Sequences:

>Translated_330_residues
MSQPIKIAISGGAGQIAYSLLFRLASGELFGPNQLIELQVLEVPNALSALEGVKMEIEDCAFPLLSSIKICSDPYQAFED
IDYALLIGAKSRGPGMERRDLLQENSKIFVNQGQALNAVAKSSAKIFVVGNPCNTNCLIALNNAPSLKRENFYAMTRLDQ
NRATFFLSQKSQVSTKDVSCVTIWGNHSATQVPDFVNAKISQKPVETIIPDRQWLEKDFIESVQKRGAAIIQARGKSSAA
SAASALLDAMRDRILPTPTGQWFSTALLSDGNPYGIEEGLIFSFPCRVKKNGELSIVSGLKWDAFLEEKIKLTEQELKEE
REMVSSILKI
>Mature_329_residues
SQPIKIAISGGAGQIAYSLLFRLASGELFGPNQLIELQVLEVPNALSALEGVKMEIEDCAFPLLSSIKICSDPYQAFEDI
DYALLIGAKSRGPGMERRDLLQENSKIFVNQGQALNAVAKSSAKIFVVGNPCNTNCLIALNNAPSLKRENFYAMTRLDQN
RATFFLSQKSQVSTKDVSCVTIWGNHSATQVPDFVNAKISQKPVETIIPDRQWLEKDFIESVQKRGAAIIQARGKSSAAS
AASALLDAMRDRILPTPTGQWFSTALLSDGNPYGIEEGLIFSFPCRVKKNGELSIVSGLKWDAFLEEKIKLTEQELKEER
EMVSSILKI

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 2 family

Homologues:

Organism=Homo sapiens, GI5174539, Length=326, Percent_Identity=47.239263803681, Blast_Score=304, Evalue=9e-83,
Organism=Homo sapiens, GI89886456, Length=320, Percent_Identity=23.75, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17561064, Length=327, Percent_Identity=47.4006116207951, Blast_Score=275, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI32566798, Length=257, Percent_Identity=47.0817120622568, Blast_Score=220, Evalue=7e-58,
Organism=Caenorhabditis elegans, GI32566800, Length=172, Percent_Identity=46.5116279069767, Blast_Score=128, Evalue=4e-30,
Organism=Drosophila melanogaster, GI24583394, Length=332, Percent_Identity=48.4939759036145, Blast_Score=291, Evalue=4e-79,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): MDH_PARUW (Q6MAA3)

Other databases:

- EMBL:   BX908798
- RefSeq:   YP_008771.1
- ProteinModelPortal:   Q6MAA3
- SMR:   Q6MAA3
- STRING:   Q6MAA3
- GeneID:   2779853
- GenomeReviews:   BX908798_GR
- KEGG:   pcu:pc1772
- NMPDR:   fig|264201.1.peg.1772
- eggNOG:   COG0039
- HOGENOM:   HBG289884
- OMA:   MIIWGNH
- ProtClustDB:   PRK05442
- BioCyc:   CPRO264201:PC1772-MONOMER
- BioCyc:   PCHL-E25-01:PCHL-E25-01-001772-MONOMER
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_01517
- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR001252
- InterPro:   IPR010945
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR23382
- PIRSF:   PIRSF000102
- TIGRFAMs:   TIGR01759

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.37

Molecular weight: Translated: 36250; Mature: 36119

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS00068 MDH

Important sites: ACT_SITE 187-187 BINDING 92-92 BINDING 98-98 BINDING 105-105 BINDING 112-112 BINDING 131-131 BINDING 162-162

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQPIKIAISGGAGQIAYSLLFRLASGELFGPNQLIELQVLEVPNALSALEGVKMEIEDC
CCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCHHHHHHCCCEEHHHH
AFPLLSSIKICSDPYQAFEDIDYALLIGAKSRGPGMERRDLLQENSKIFVNQGQALNAVA
HHHHHHHHHHCCCHHHHHHCCCEEEEEECCCCCCCCHHHHHHHCCCEEEEECCCHHHHHH
KSSAKIFVVGNPCNTNCLIALNNAPSLKRENFYAMTRLDQNRATFFLSQKSQVSTKDVSC
HCCCEEEEEECCCCCEEEEEECCCCCCCCCCEEEEEEECCCCEEEEEECCCCCCCCCCEE
VTIWGNHSATQVPDFVNAKISQKPVETIIPDRQWLEKDFIESVQKRGAAIIQARGKSSAA
EEEECCCCCCCCCHHHCCCCCCCCHHHHCCCHHHHHHHHHHHHHHCCCEEEEECCCCHHH
SAASALLDAMRDRILPTPTGQWFSTALLSDGNPYGIEEGLIFSFPCRVKKNGELSIVSGL
HHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCEEECCCEECCCCCEEEEECC
KWDAFLEEKIKLTEQELKEEREMVSSILKI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SQPIKIAISGGAGQIAYSLLFRLASGELFGPNQLIELQVLEVPNALSALEGVKMEIEDC
CCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCHHHHHHCCCEEHHHH
AFPLLSSIKICSDPYQAFEDIDYALLIGAKSRGPGMERRDLLQENSKIFVNQGQALNAVA
HHHHHHHHHHCCCHHHHHHCCCEEEEEECCCCCCCCHHHHHHHCCCEEEEECCCHHHHHH
KSSAKIFVVGNPCNTNCLIALNNAPSLKRENFYAMTRLDQNRATFFLSQKSQVSTKDVSC
HCCCEEEEEECCCCCEEEEEECCCCCCCCCCEEEEEEECCCCEEEEEECCCCCCCCCCEE
VTIWGNHSATQVPDFVNAKISQKPVETIIPDRQWLEKDFIESVQKRGAAIIQARGKSSAA
EEEECCCCCCCCCHHHCCCCCCCCHHHHCCCHHHHHHHHHHHHHHCCCEEEEECCCCHHH
SAASALLDAMRDRILPTPTGQWFSTALLSDGNPYGIEEGLIFSFPCRVKKNGELSIVSGL
HHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCEEECCCEECCCCCEEEEECC
KWDAFLEEKIKLTEQELKEEREMVSSILKI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA