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The map label for this gene is ligA

Identifier: 46447394

GI number: 46447394

Start: 2103293

End: 2105290

Strand: Reverse

Name: ligA

Synonym: pc1760

Alternate gene names: 46447394

Gene position: 2105290-2103293 (Counterclockwise)

Preceding gene: 46447395

Following gene: 46447393

Centisome position: 87.19

GC content: 36.54

Gene sequence:

>1998_bases
ATGATCACGCAAAAAGATTATGAAAAACTTTGTCATGAAATTTGGCATCATAACAAACTTTATTACATTGAACATCAACC
CATTATTTCTGATGAAGAATTCGATGCTCTTTTAAAAAAGTTGGAAGAAATTGAGCGATCGCATCCTGAATGGATCACGG
AATTTTCTCCCTCTCAACGCGTTAATGAAAGTTTAACAAGTGGTTTTAAGACAGTTGCACATCGTACTCCTATGTTGTCT
TTAGCAAATACGTATTCTAAGGAAGAAATTGAGGATTTTATTAAACGCCTTCAAAAACTCGTCGGAAAACGACAAGTAGA
ATTTTCTGTAGAGTTAAAAATGGATGGGATTGCAATTACTGCTATTTATGAACAAGGGATTTTTAAGCGCGGAATTACAA
GAGGAAATGGAAAAAGAGGAGATGACATCACTACTAATATGCGAATGATTGAAAATCTTCCTCTTCAACTTAGTGGAGAA
AATCTTCCTGATTTCTTAGAAATTAGGGGAGAAGTTTTCATGCCACGACAAGTATTTCTGCAATTAAACGAACAAAAGTT
ACAAGATGGAGAAGTACTCTGGGCTAACCCACGCAATGCTGCAGCAGGATCTTTAAAACTCTTGGATCCAAAAATGGTTG
CGGAACGAAGGCTAGCTGTTGTTTTCTATGGTCTTGCTGAAGATTCTTCTGCCTCTATCAAAAAGCAAGCTGAAGTTCCT
TCATTTTTTCGATCTATTGGTCTTCCGGCATTAGAACATCACGCATATTGTCAAAATATTGAACAAATATGGAAATTTGC
TGAAGAAATTCGATCTTTGCGAACTATTCTTCCTTATGATATCGATGGGATTGTTATCAAATTAAATGATTTTAAAGATC
AAAAAAGATTGGGTGTCACAGGCAAAAGCCCTCGTTGGGCTATTGCTTATAAATTTGCTGCTGAACAAGCAAAAACCAGA
ATTATTGACATCACTGTTCAAATTGGAAGAACGGGTGTTTTGACTCCAGTTGCAGAATTAGAGCCTATCTTTTTGTCAGG
AAGTACGATTGCGCGAGCCTCCTTATATAATCAAGAAGAAGTGCAACGAAAAGATATTCGAATTGGAGATTTAGTCACTA
TTGAAAAGGGAGGAGATGTCATTCCCAAAGTTTTGAATGTCGAATTATCTCAACGTCCTTTACATTCCCAACCTTGGCAA
ATGCCTCTTTACTGTCCTTCTTGCGGAACGCAAGTCATTAATATCATAGGAGAGGTAGCCGTTCGATGTCCCAATGAAGA
TTCTTGTACTGAACAACAAATTAGAAAATTGATCTATTTTGTGGGAAAGCAAGCTATGGATATTAAACATATGGGAGAAA
AAATCGTCATTCAGTTATTTCAAAAAGGATTTATTCATCTTCCATCTGATATATTTGCCTTAACTGAAGGGCAAATTTCG
CAGTTGACGAATTTTAAAACTAAAGCCATACAAAATTTAATGAGAAGTATTGAAGAGTCTAAACATGTCTCTTTGGAACG
TTTTATTATGGCCCTTGAAATTAAATATATAGGAATAGGGACGGCCGAATTATTAGCGGCAAGAGCTGGGACTATTGAAA
CGCTTATGCAATTAAACGAGGAAGATCTTATCAAAATAGAGGGAGTGGGCGGAAAAGTTGCACAGGCCGTGGTTGAACAT
TTTCAAAATCCAAAGCATCGGCAAGAAGTTTATCGATTATTAGAATTAGGTGTTTGTCCCCAATCTAAAACAGTTCAAAT
TTTTACGAACCATGCTTTTCAAGGAAAAATTTTCGTACTTACAGGGAGTTTGGAACATTATACAAGACAAAGTGCTGCTA
GTTTGATTAAAGAACGAGGGGGAAAAGTTTCAGATTCAGTTAGCAGAAAAACCAATTATGTGGTTGCAGGAGCCGAGCCT
GGATCTAAGCTTGACAAAGCCCGAACATTAGGAATTCCAGTTCTCAACGAAAAAGAATTTATAAGTCTTTGTCATTAG

Upstream 100 bases:

>100_bases
ACTCAATATCGATTCACATGCAAATTTGAAGCGAAAAACTCAACTTCTCGATTCGAAATATTAAATAACATAATAAAATT
AAAATTTAGGTTATGTCTCA

Downstream 100 bases:

>100_bases
CTTTTAAAAAAAACTATTGAGAGTAGGTTAATTTTTTATTTGCTTTAGAAATCCTCTAAAGTTTATGCATAAAGCTGATA
AAAATGAGAATTTGAAGAAT

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 665; Mature: 665

Protein sequence:

>665_residues
MITQKDYEKLCHEIWHHNKLYYIEHQPIISDEEFDALLKKLEEIERSHPEWITEFSPSQRVNESLTSGFKTVAHRTPMLS
LANTYSKEEIEDFIKRLQKLVGKRQVEFSVELKMDGIAITAIYEQGIFKRGITRGNGKRGDDITTNMRMIENLPLQLSGE
NLPDFLEIRGEVFMPRQVFLQLNEQKLQDGEVLWANPRNAAAGSLKLLDPKMVAERRLAVVFYGLAEDSSASIKKQAEVP
SFFRSIGLPALEHHAYCQNIEQIWKFAEEIRSLRTILPYDIDGIVIKLNDFKDQKRLGVTGKSPRWAIAYKFAAEQAKTR
IIDITVQIGRTGVLTPVAELEPIFLSGSTIARASLYNQEEVQRKDIRIGDLVTIEKGGDVIPKVLNVELSQRPLHSQPWQ
MPLYCPSCGTQVINIIGEVAVRCPNEDSCTEQQIRKLIYFVGKQAMDIKHMGEKIVIQLFQKGFIHLPSDIFALTEGQIS
QLTNFKTKAIQNLMRSIEESKHVSLERFIMALEIKYIGIGTAELLAARAGTIETLMQLNEEDLIKIEGVGGKVAQAVVEH
FQNPKHRQEVYRLLELGVCPQSKTVQIFTNHAFQGKIFVLTGSLEHYTRQSAASLIKERGGKVSDSVSRKTNYVVAGAEP
GSKLDKARTLGIPVLNEKEFISLCH

Sequences:

>Translated_665_residues
MITQKDYEKLCHEIWHHNKLYYIEHQPIISDEEFDALLKKLEEIERSHPEWITEFSPSQRVNESLTSGFKTVAHRTPMLS
LANTYSKEEIEDFIKRLQKLVGKRQVEFSVELKMDGIAITAIYEQGIFKRGITRGNGKRGDDITTNMRMIENLPLQLSGE
NLPDFLEIRGEVFMPRQVFLQLNEQKLQDGEVLWANPRNAAAGSLKLLDPKMVAERRLAVVFYGLAEDSSASIKKQAEVP
SFFRSIGLPALEHHAYCQNIEQIWKFAEEIRSLRTILPYDIDGIVIKLNDFKDQKRLGVTGKSPRWAIAYKFAAEQAKTR
IIDITVQIGRTGVLTPVAELEPIFLSGSTIARASLYNQEEVQRKDIRIGDLVTIEKGGDVIPKVLNVELSQRPLHSQPWQ
MPLYCPSCGTQVINIIGEVAVRCPNEDSCTEQQIRKLIYFVGKQAMDIKHMGEKIVIQLFQKGFIHLPSDIFALTEGQIS
QLTNFKTKAIQNLMRSIEESKHVSLERFIMALEIKYIGIGTAELLAARAGTIETLMQLNEEDLIKIEGVGGKVAQAVVEH
FQNPKHRQEVYRLLELGVCPQSKTVQIFTNHAFQGKIFVLTGSLEHYTRQSAASLIKERGGKVSDSVSRKTNYVVAGAEP
GSKLDKARTLGIPVLNEKEFISLCH
>Mature_665_residues
MITQKDYEKLCHEIWHHNKLYYIEHQPIISDEEFDALLKKLEEIERSHPEWITEFSPSQRVNESLTSGFKTVAHRTPMLS
LANTYSKEEIEDFIKRLQKLVGKRQVEFSVELKMDGIAITAIYEQGIFKRGITRGNGKRGDDITTNMRMIENLPLQLSGE
NLPDFLEIRGEVFMPRQVFLQLNEQKLQDGEVLWANPRNAAAGSLKLLDPKMVAERRLAVVFYGLAEDSSASIKKQAEVP
SFFRSIGLPALEHHAYCQNIEQIWKFAEEIRSLRTILPYDIDGIVIKLNDFKDQKRLGVTGKSPRWAIAYKFAAEQAKTR
IIDITVQIGRTGVLTPVAELEPIFLSGSTIARASLYNQEEVQRKDIRIGDLVTIEKGGDVIPKVLNVELSQRPLHSQPWQ
MPLYCPSCGTQVINIIGEVAVRCPNEDSCTEQQIRKLIYFVGKQAMDIKHMGEKIVIQLFQKGFIHLPSDIFALTEGQIS
QLTNFKTKAIQNLMRSIEESKHVSLERFIMALEIKYIGIGTAELLAARAGTIETLMQLNEEDLIKIEGVGGKVAQAVVEH
FQNPKHRQEVYRLLELGVCPQSKTVQIFTNHAFQGKIFVLTGSLEHYTRQSAASLIKERGGKVSDSVSRKTNYVVAGAEP
GSKLDKARTLGIPVLNEKEFISLCH

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=658, Percent_Identity=40.7294832826748, Blast_Score=517, Evalue=1e-148,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_PARUW (Q6MAB5)

Other databases:

- EMBL:   BX908798
- RefSeq:   YP_008759.1
- HSSP:   P26996
- ProteinModelPortal:   Q6MAB5
- STRING:   Q6MAB5
- GeneID:   2779561
- GenomeReviews:   BX908798_GR
- KEGG:   pcu:pc1760
- NMPDR:   fig|264201.1.peg.1760
- eggNOG:   COG0272
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- PhylomeDB:   Q6MAB5
- ProtClustDB:   PRK07956
- BioCyc:   CPRO264201:PC1760-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 75279; Mature: 75279

Theoretical pI: Translated: 7.64; Mature: 7.64

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 113-113 BINDING 111-111 BINDING 134-134 BINDING 171-171 BINDING 287-287 BINDING 311-311

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITQKDYEKLCHEIWHHNKLYYIEHQPIISDEEFDALLKKLEEIERSHPEWITEFSPSQR
CCCHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCHHHCCCCHHH
VNESLTSGFKTVAHRTPMLSLANTYSKEEIEDFIKRLQKLVGKRQVEFSVELKMDGIAIT
HHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEEEEEEECCEEEE
AIYEQGIFKRGITRGNGKRGDDITTNMRMIENLPLQLSGENLPDFLEIRGEVFMPRQVFL
EEHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCEECCCCCCHHHHHCCCEECCHHHHH
QLNEQKLQDGEVLWANPRNAAAGSLKLLDPKMVAERRLAVVFYGLAEDSSASIKKQAEVP
HHCHHHCCCCCEEEECCCCCCCCCEEECCHHHHHHCCEEEEEEEECCCCCCHHHHHHHHH
SFFRSIGLPALEHHAYCQNIEQIWKFAEEIRSLRTILPYDIDGIVIKLNDFKDQKRLGVT
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHCCCC
GKSPRWAIAYKFAAEQAKTRIIDITVQIGRTGVLTPVAELEPIFLSGSTIARASLYNQEE
CCCCCEEEEEEHHHHHHCCEEEEEEEEECCCCCCCCHHHCCEEEECCCHHHHHHHCCHHH
VQRKDIRIGDLVTIEKGGDVIPKVLNVELSQRPLHSQPWQMPLYCPSCGTQVINIIGEVA
HHHHCCCCCCEEEECCCCCCCCHHHCCCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHH
VRCPNEDSCTEQQIRKLIYFVGKQAMDIKHMGEKIVIQLFQKGFIHLPSDIFALTEGQIS
EECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEECCCHHHHCCCCHHH
QLTNFKTKAIQNLMRSIEESKHVSLERFIMALEIKYIGIGTAELLAARAGTIETLMQLNE
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHEEEEECCCHHHHHHHCCCHHHHHHHCCC
EDLIKIEGVGGKVAQAVVEHFQNPKHRQEVYRLLELGVCPQSKTVQIFTNHAFQGKIFVL
CCEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEEEE
TGSLEHYTRQSAASLIKERGGKVSDSVSRKTNYVVAGAEPGSKLDKARTLGIPVLNEKEF
ECCHHHHHHHHHHHHHHHCCCCCCHHHHCCCCEEEEECCCCCHHHHHHHCCCCCCCCHHH
ISLCH
HHHCC
>Mature Secondary Structure
MITQKDYEKLCHEIWHHNKLYYIEHQPIISDEEFDALLKKLEEIERSHPEWITEFSPSQR
CCCHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCHHHCCCCHHH
VNESLTSGFKTVAHRTPMLSLANTYSKEEIEDFIKRLQKLVGKRQVEFSVELKMDGIAIT
HHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEEEEEEECCEEEE
AIYEQGIFKRGITRGNGKRGDDITTNMRMIENLPLQLSGENLPDFLEIRGEVFMPRQVFL
EEHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCEECCCCCCHHHHHCCCEECCHHHHH
QLNEQKLQDGEVLWANPRNAAAGSLKLLDPKMVAERRLAVVFYGLAEDSSASIKKQAEVP
HHCHHHCCCCCEEEECCCCCCCCCEEECCHHHHHHCCEEEEEEEECCCCCCHHHHHHHHH
SFFRSIGLPALEHHAYCQNIEQIWKFAEEIRSLRTILPYDIDGIVIKLNDFKDQKRLGVT
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHCCCC
GKSPRWAIAYKFAAEQAKTRIIDITVQIGRTGVLTPVAELEPIFLSGSTIARASLYNQEE
CCCCCEEEEEEHHHHHHCCEEEEEEEEECCCCCCCCHHHCCEEEECCCHHHHHHHCCHHH
VQRKDIRIGDLVTIEKGGDVIPKVLNVELSQRPLHSQPWQMPLYCPSCGTQVINIIGEVA
HHHHCCCCCCEEEECCCCCCCCHHHCCCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHH
VRCPNEDSCTEQQIRKLIYFVGKQAMDIKHMGEKIVIQLFQKGFIHLPSDIFALTEGQIS
EECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEECCCHHHHCCCCHHH
QLTNFKTKAIQNLMRSIEESKHVSLERFIMALEIKYIGIGTAELLAARAGTIETLMQLNE
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHEEEEECCCHHHHHHHCCCHHHHHHHCCC
EDLIKIEGVGGKVAQAVVEHFQNPKHRQEVYRLLELGVCPQSKTVQIFTNHAFQGKIFVL
CCEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEEEE
TGSLEHYTRQSAASLIKERGGKVSDSVSRKTNYVVAGAEPGSKLDKARTLGIPVLNEKEF
ECCHHHHHHHHHHHHHHHCCCCCCHHHHCCCCEEEEECCCCCHHHHHHHCCCCCCCCHHH
ISLCH
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA