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The map label for this gene is pdhC [H]

Identifier: 46447365

GI number: 46447365

Start: 2070586

End: 2071887

Strand: Reverse

Name: pdhC [H]

Synonym: pc1731

Alternate gene names: 46447365

Gene position: 2071887-2070586 (Counterclockwise)

Preceding gene: 46447366

Following gene: 46447364

Centisome position: 85.81

GC content: 37.4

Gene sequence:

>1302_bases
ATGCCTTTTACACTGACAATGCCCAAACTCTCTCCCACAATGGAAGAAGGAACACTGATCAAGTGGCATAAAAAAATTGG
GGATTCGATACAAACTGGCGACTTGCTAATTGAAGTAGCTACTGATAAAGCAACTGTAGAATATAATGCTATTGATGACG
GATGGCTTAGGCAGATTTTAATTCAGGAAGGAAAAGATGCTGCTGTTAATCAAGCTATAGCGATTTTAACCGTCGATCAG
AATGAAAGTTTAGAAGGTTACCAAGCAGACGGGGTAAAAGAAAAGGCTTTGCAACTTTCATCTGACAGCATTGAGATGCC
AGAGTTAAATTACAAAGAGAAGAAAGAACCTAAAAGTAAAACTACGGCTTTTCAACAGCCTGTCTTTGTCCCAGAATTTC
CTTTGGAAAATTATACGTTTGAATTCCCGATAGAAAGAGGGGATAAAAAATTATTAGCTTCCCCCTTAGCTAAAAAACTC
GCTAAAGAAAAAGGATTAGATCTTACAACAGTAAAAGGAACAGGTCCTCAACAACGAATTATAAGTCGAGATCTTGACAA
AGCTCAAGCTGCTGGAGTTGTCAATTTTGGACATCGTGAGACTCCTCAATTACCTCCAGGTAGTTATGAAGAATTATCTT
TGACTCCTATGCGTAAAGTTATCGGACAAAGGTTACAAGAATCTAAATCCTTTATTCCTCATTTTTATGTCACTTTAACA
ATCGATGCTAGTCCATTGACCCAAATCAGAGAGCAACTTAAAAATAATCAAGTTAAAGTATCTATCAATGATTTCATTGT
TCGAGCCTGTGCATTAGCTTTACGTCAGAATCCCGGTTTAAATTGTGGTTTCAATTCTGCGAATCAATCGATTATTCAAT
TTAAAACAATTGATATTGCAGTTGCTGTTAGCCTGGAAGAAGGGCTAATCACTCCTATTATTCGTCACGCAGACTTTAAA
AATTTAGGAGAACTGTCGGTAGAAATGCGTGTGTTGGCTCAGAAAGCACGTGAAGGAAAGCTTGAACCTCAAGAATATAA
AGGAGGATCATTTACGATTTCAAATTTAGGAATGTTTGGTGTTTCCGAGTTTCAAGCGATACTAAATCCTCCACAAGCGG
CTATTCTCGCTGTGAGTGGAATTTTAGATGTTCCTGTTATTCAAAATAACATGGTGATACCCGGTAAAACAATGAATTTA
ACTTTATCAGTTGATCACCGTGTAATAGATGGGGTGGCGGCTGCCAAATTTCTTCAAAGTTTAAAGCAATTGTTAGAAAA
TCCCGCTGGATTATTGCTTTAA

Upstream 100 bases:

>100_bases
GCCTTATTCGAAGGTATTAGAAAAAGAAACAATGCCAAATAAACAAAGAATTCTTTCTGCAATTTATAAAACACTTCAAA
AAAATTAGGAGTTAAGTGGT

Downstream 100 bases:

>100_bases
TAAACAAGTAAAATGAGGTTTAAAAATTGATCACGTTAAACCTACGTAAAAAATGCTCTATAGGAAAAAATAGAAACTTT
GTAATATGATTTAAAATAAA

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MPFTLTMPKLSPTMEEGTLIKWHKKIGDSIQTGDLLIEVATDKATVEYNAIDDGWLRQILIQEGKDAAVNQAIAILTVDQ
NESLEGYQADGVKEKALQLSSDSIEMPELNYKEKKEPKSKTTAFQQPVFVPEFPLENYTFEFPIERGDKKLLASPLAKKL
AKEKGLDLTTVKGTGPQQRIISRDLDKAQAAGVVNFGHRETPQLPPGSYEELSLTPMRKVIGQRLQESKSFIPHFYVTLT
IDASPLTQIREQLKNNQVKVSINDFIVRACALALRQNPGLNCGFNSANQSIIQFKTIDIAVAVSLEEGLITPIIRHADFK
NLGELSVEMRVLAQKAREGKLEPQEYKGGSFTISNLGMFGVSEFQAILNPPQAAILAVSGILDVPVIQNNMVIPGKTMNL
TLSVDHRVIDGVAAAKFLQSLKQLLENPAGLLL

Sequences:

>Translated_433_residues
MPFTLTMPKLSPTMEEGTLIKWHKKIGDSIQTGDLLIEVATDKATVEYNAIDDGWLRQILIQEGKDAAVNQAIAILTVDQ
NESLEGYQADGVKEKALQLSSDSIEMPELNYKEKKEPKSKTTAFQQPVFVPEFPLENYTFEFPIERGDKKLLASPLAKKL
AKEKGLDLTTVKGTGPQQRIISRDLDKAQAAGVVNFGHRETPQLPPGSYEELSLTPMRKVIGQRLQESKSFIPHFYVTLT
IDASPLTQIREQLKNNQVKVSINDFIVRACALALRQNPGLNCGFNSANQSIIQFKTIDIAVAVSLEEGLITPIIRHADFK
NLGELSVEMRVLAQKAREGKLEPQEYKGGSFTISNLGMFGVSEFQAILNPPQAAILAVSGILDVPVIQNNMVIPGKTMNL
TLSVDHRVIDGVAAAKFLQSLKQLLENPAGLLL
>Mature_432_residues
PFTLTMPKLSPTMEEGTLIKWHKKIGDSIQTGDLLIEVATDKATVEYNAIDDGWLRQILIQEGKDAAVNQAIAILTVDQN
ESLEGYQADGVKEKALQLSSDSIEMPELNYKEKKEPKSKTTAFQQPVFVPEFPLENYTFEFPIERGDKKLLASPLAKKLA
KEKGLDLTTVKGTGPQQRIISRDLDKAQAAGVVNFGHRETPQLPPGSYEELSLTPMRKVIGQRLQESKSFIPHFYVTLTI
DASPLTQIREQLKNNQVKVSINDFIVRACALALRQNPGLNCGFNSANQSIIQFKTIDIAVAVSLEEGLITPIIRHADFKN
LGELSVEMRVLAQKAREGKLEPQEYKGGSFTISNLGMFGVSEFQAILNPPQAAILAVSGILDVPVIQNNMVIPGKTMNLT
LSVDHRVIDGVAAAKFLQSLKQLLENPAGLLL

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=442, Percent_Identity=37.7828054298643, Blast_Score=268, Evalue=5e-72,
Organism=Homo sapiens, GI203098816, Length=469, Percent_Identity=33.6886993603412, Blast_Score=243, Evalue=2e-64,
Organism=Homo sapiens, GI203098753, Length=469, Percent_Identity=33.6886993603412, Blast_Score=243, Evalue=2e-64,
Organism=Homo sapiens, GI110671329, Length=455, Percent_Identity=26.3736263736264, Blast_Score=139, Evalue=5e-33,
Organism=Homo sapiens, GI19923748, Length=222, Percent_Identity=35.5855855855856, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI260898739, Length=160, Percent_Identity=44.375, Blast_Score=130, Evalue=2e-30,
Organism=Escherichia coli, GI1786946, Length=438, Percent_Identity=29.9086757990868, Blast_Score=175, Evalue=4e-45,
Organism=Escherichia coli, GI1786305, Length=309, Percent_Identity=33.3333333333333, Blast_Score=156, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI17560088, Length=450, Percent_Identity=39.5555555555556, Blast_Score=286, Evalue=1e-77,
Organism=Caenorhabditis elegans, GI17538894, Length=300, Percent_Identity=34.6666666666667, Blast_Score=157, Evalue=7e-39,
Organism=Caenorhabditis elegans, GI25146366, Length=438, Percent_Identity=28.5388127853881, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17537937, Length=432, Percent_Identity=26.1574074074074, Blast_Score=144, Evalue=6e-35,
Organism=Saccharomyces cerevisiae, GI6324258, Length=456, Percent_Identity=33.1140350877193, Blast_Score=231, Evalue=1e-61,
Organism=Saccharomyces cerevisiae, GI6320352, Length=233, Percent_Identity=33.4763948497854, Blast_Score=140, Evalue=5e-34,
Organism=Saccharomyces cerevisiae, GI6321632, Length=80, Percent_Identity=45, Blast_Score=74, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24582497, Length=295, Percent_Identity=38.6440677966102, Blast_Score=197, Evalue=1e-50,
Organism=Drosophila melanogaster, GI20129315, Length=295, Percent_Identity=38.6440677966102, Blast_Score=197, Evalue=1e-50,
Organism=Drosophila melanogaster, GI18859875, Length=440, Percent_Identity=28.8636363636364, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24645909, Length=227, Percent_Identity=32.15859030837, Blast_Score=122, Evalue=5e-28,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006257
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 47700; Mature: 47568

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS50968 BIOTINYL_LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFTLTMPKLSPTMEEGTLIKWHKKIGDSIQTGDLLIEVATDKATVEYNAIDDGWLRQIL
CCEEEECCCCCCCCCCCCEEEEHHHHCCCCCCCCEEEEEECCCEEEEECCCCHHHHHHHH
IQEGKDAAVNQAIAILTVDQNESLEGYQADGVKEKALQLSSDSIEMPELNYKEKKEPKSK
HHCCCCHHHCCEEEEEEECCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCHHCCCCHH
TTAFQQPVFVPEFPLENYTFEFPIERGDKKLLASPLAKKLAKEKGLDLTTVKGTGPQQRI
HHHHHCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHH
ISRDLDKAQAAGVVNFGHRETPQLPPGSYEELSLTPMRKVIGQRLQESKSFIPHFYVTLT
HHHHHHHHHHCCEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEE
IDASPLTQIREQLKNNQVKVSINDFIVRACALALRQNPGLNCGFNSANQSIIQFKTIDIA
ECCCHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEEE
VAVSLEEGLITPIIRHADFKNLGELSVEMRVLAQKAREGKLEPQEYKGGSFTISNLGMFG
EEEEECCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHCCCCCEEEECCCCCC
VSEFQAILNPPQAAILAVSGILDVPVIQNNMVIPGKTMNLTLSVDHRVIDGVAAAKFLQS
HHHHHHHHCCCHHHHEEHHCCCCCCEECCCEECCCCEEEEEEEECHHHHHHHHHHHHHHH
LKQLLENPAGLLL
HHHHHCCCCCCCC
>Mature Secondary Structure 
PFTLTMPKLSPTMEEGTLIKWHKKIGDSIQTGDLLIEVATDKATVEYNAIDDGWLRQIL
CEEEECCCCCCCCCCCCEEEEHHHHCCCCCCCCEEEEEECCCEEEEECCCCHHHHHHHH
IQEGKDAAVNQAIAILTVDQNESLEGYQADGVKEKALQLSSDSIEMPELNYKEKKEPKSK
HHCCCCHHHCCEEEEEEECCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCHHCCCCHH
TTAFQQPVFVPEFPLENYTFEFPIERGDKKLLASPLAKKLAKEKGLDLTTVKGTGPQQRI
HHHHHCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHH
ISRDLDKAQAAGVVNFGHRETPQLPPGSYEELSLTPMRKVIGQRLQESKSFIPHFYVTLT
HHHHHHHHHHCCEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEE
IDASPLTQIREQLKNNQVKVSINDFIVRACALALRQNPGLNCGFNSANQSIIQFKTIDIA
ECCCHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEEE
VAVSLEEGLITPIIRHADFKNLGELSVEMRVLAQKAREGKLEPQEYKGGSFTISNLGMFG
EEEEECCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHCCCCCEEEECCCCCC
VSEFQAILNPPQAAILAVSGILDVPVIQNNMVIPGKTMNLTLSVDHRVIDGVAAAKFLQS
HHHHHHHHCCCHHHHEEHHCCCCCCEECCCEECCCCEEEEEEEECHHHHHHHHHHHHHHH
LKQLLENPAGLLL
HHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA