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The map label for this gene is pdhB [H]

Identifier: 46447366

GI number: 46447366

Start: 2071900

End: 2072892

Strand: Reverse

Name: pdhB [H]

Synonym: pc1732

Alternate gene names: 46447366

Gene position: 2072892-2071900 (Counterclockwise)

Preceding gene: 46447367

Following gene: 46447365

Centisome position: 85.85

GC content: 36.66

Gene sequence:

>993_bases
ATGAGCACTGAAAAGCAAACTATCGACATTAGAGAAGCGTTACGACAAGCAATTAATGAGGAAATGGCAAGGGATTCTAG
CGTTTTTGTCATGGGGGAAGAAGTTGGCGAATATAATGGAGCGTATAAAATTACTAAAGGAATGTTGGATAAATGGGGAG
CAAATCGCATCATAGATACCCCTATTTCCGAATTAGGTTTTGCTGGTTTGTGTATAGGCGCTGCTATGACTGGACTTCGA
CCAATCGTTGAATTTATGAGTTTTAATTTTTCATTTGTTGCAGCCGATCAATTGATTTCTAACGCGATTAAAATGTACTA
TATGTCAGGTAATCGTTTTTCTGTTCCCATTGTTTTTAGAGGGCCTAATGGAGCGGCAGCACAAGTTTCCAGTCAACATT
CTCATTGTGTAGAAGCTATTTATGGAAATTTACCAGGATGGACTATTATAGCTCCAAGCAATGCTTATGATGCGAAAGGA
TTATTAAAGTCTGCTATTCGAGATAATAATCCTGTTTTATTTCTAGAATCAGAGCTTTCTTATGGAGATAAGATGGAAAT
TCCGGTCGATGAATATCTAATTCCGATTGGGAAAGCACAGATTGTTGTGCCTGGGGCAGATGTGACTTTAATTGCTCATA
GCCGCATGGTTACAATTTGTAAAGAAGTTGTAAATGAATTAACAAAAATGGGAATTTGTGCTGAACTTATTGATCTTCGG
ACAGTCAAACCTTTAGATATTGCTACTATTGCAAATTCAGTAAAAAAGACTAATCGCTGTGTCATTGTTGAAGAAGGACA
TTTATTTGCAGGCATTGCGGCGGAAGTTGGCTTTCAAATTATGGAACATTGCTTTGATTATTTAGATGCTCCTTTGGAAA
GAGTTTGCCAACGCGAAACACCCATGCCTTATTCGAAGGTATTAGAAAAAGAAACAATGCCAAATAAACAAAGAATTCTT
TCTGCAATTTATAAAACACTTCAAAAAAATTAG

Upstream 100 bases:

>100_bases
TTGAGGCAATGTCTTTTGCAGAAAATAGCCCATGGCCTGATCCGCAAACATTGGAAGAAGACGTATTCGCCCCCAATTTC
ATTGTATAGGAGTATACTCA

Downstream 100 bases:

>100_bases
GAGTTAAGTGGTATGCCTTTTACACTGACAATGCCCAAACTCTCTCCCACAATGGAAGAAGGAACACTGATCAAGTGGCA
TAAAAAAATTGGGGATTCGA

Product: pyruvate dehydrogenase (lipoamide), E1 component, beta chain

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MSTEKQTIDIREALRQAINEEMARDSSVFVMGEEVGEYNGAYKITKGMLDKWGANRIIDTPISELGFAGLCIGAAMTGLR
PIVEFMSFNFSFVAADQLISNAIKMYYMSGNRFSVPIVFRGPNGAAAQVSSQHSHCVEAIYGNLPGWTIIAPSNAYDAKG
LLKSAIRDNNPVLFLESELSYGDKMEIPVDEYLIPIGKAQIVVPGADVTLIAHSRMVTICKEVVNELTKMGICAELIDLR
TVKPLDIATIANSVKKTNRCVIVEEGHLFAGIAAEVGFQIMEHCFDYLDAPLERVCQRETPMPYSKVLEKETMPNKQRIL
SAIYKTLQKN

Sequences:

>Translated_330_residues
MSTEKQTIDIREALRQAINEEMARDSSVFVMGEEVGEYNGAYKITKGMLDKWGANRIIDTPISELGFAGLCIGAAMTGLR
PIVEFMSFNFSFVAADQLISNAIKMYYMSGNRFSVPIVFRGPNGAAAQVSSQHSHCVEAIYGNLPGWTIIAPSNAYDAKG
LLKSAIRDNNPVLFLESELSYGDKMEIPVDEYLIPIGKAQIVVPGADVTLIAHSRMVTICKEVVNELTKMGICAELIDLR
TVKPLDIATIANSVKKTNRCVIVEEGHLFAGIAAEVGFQIMEHCFDYLDAPLERVCQRETPMPYSKVLEKETMPNKQRIL
SAIYKTLQKN
>Mature_329_residues
STEKQTIDIREALRQAINEEMARDSSVFVMGEEVGEYNGAYKITKGMLDKWGANRIIDTPISELGFAGLCIGAAMTGLRP
IVEFMSFNFSFVAADQLISNAIKMYYMSGNRFSVPIVFRGPNGAAAQVSSQHSHCVEAIYGNLPGWTIIAPSNAYDAKGL
LKSAIRDNNPVLFLESELSYGDKMEIPVDEYLIPIGKAQIVVPGADVTLIAHSRMVTICKEVVNELTKMGICAELIDLRT
VKPLDIATIANSVKKTNRCVIVEEGHLFAGIAAEVGFQIMEHCFDYLDAPLERVCQRETPMPYSKVLEKETMPNKQRILS
AIYKTLQKN

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156564403, Length=325, Percent_Identity=52.6153846153846, Blast_Score=350, Evalue=1e-96,
Organism=Homo sapiens, GI291084858, Length=325, Percent_Identity=48.9230769230769, Blast_Score=316, Evalue=2e-86,
Organism=Homo sapiens, GI4557353, Length=327, Percent_Identity=34.8623853211009, Blast_Score=220, Evalue=1e-57,
Organism=Homo sapiens, GI34101272, Length=327, Percent_Identity=34.8623853211009, Blast_Score=220, Evalue=1e-57,
Organism=Caenorhabditis elegans, GI17538422, Length=326, Percent_Identity=50.920245398773, Blast_Score=347, Evalue=4e-96,
Organism=Caenorhabditis elegans, GI17506935, Length=328, Percent_Identity=33.8414634146341, Blast_Score=173, Evalue=9e-44,
Organism=Saccharomyces cerevisiae, GI6319698, Length=331, Percent_Identity=47.1299093655589, Blast_Score=317, Evalue=1e-87,
Organism=Drosophila melanogaster, GI21358145, Length=327, Percent_Identity=49.2354740061162, Blast_Score=339, Evalue=1e-93,
Organism=Drosophila melanogaster, GI24650940, Length=327, Percent_Identity=49.2354740061162, Blast_Score=339, Evalue=1e-93,
Organism=Drosophila melanogaster, GI160714832, Length=327, Percent_Identity=36.3914373088685, Blast_Score=198, Evalue=5e-51,
Organism=Drosophila melanogaster, GI160714828, Length=327, Percent_Identity=36.3914373088685, Blast_Score=197, Evalue=6e-51,
Organism=Drosophila melanogaster, GI24650943, Length=92, Percent_Identity=53.2608695652174, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24650945, Length=92, Percent_Identity=53.2608695652174, Blast_Score=123, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR000089
- InterPro:   IPR011053
- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 36470; Mature: 36339

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTEKQTIDIREALRQAINEEMARDSSVFVMGEEVGEYNGAYKITKGMLDKWGANRIIDT
CCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHCCCCCCHHHHHHHHHCCCCCCCCC
PISELGFAGLCIGAAMTGLRPIVEFMSFNFSFVAADQLISNAIKMYYMSGNRFSVPIVFR
CHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHEEEECCCEEEEEEEEE
GPNGAAAQVSSQHSHCVEAIYGNLPGWTIIAPSNAYDAKGLLKSAIRDNNPVLFLESELS
CCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHCCCCCEEEEECCCC
YGDKMEIPVDEYLIPIGKAQIVVPGADVTLIAHSRMVTICKEVVNELTKMGICAELIDLR
CCCCCCCCHHHHEECCCCEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHC
TVKPLDIATIANSVKKTNRCVIVEEGHLFAGIAAEVGFQIMEHCFDYLDAPLERVCQRET
CCCCCHHHHHHHHHHHCCCEEEEECCCEEEHHHHHHHHHHHHHHHHHHCHHHHHHHHCCC
PMPYSKVLEKETMPNKQRILSAIYKTLQKN
CCCHHHHHHHHCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
STEKQTIDIREALRQAINEEMARDSSVFVMGEEVGEYNGAYKITKGMLDKWGANRIIDT
CCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHCCCCCCHHHHHHHHHCCCCCCCCC
PISELGFAGLCIGAAMTGLRPIVEFMSFNFSFVAADQLISNAIKMYYMSGNRFSVPIVFR
CHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHEEEECCCEEEEEEEEE
GPNGAAAQVSSQHSHCVEAIYGNLPGWTIIAPSNAYDAKGLLKSAIRDNNPVLFLESELS
CCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHCCCCCEEEEECCCC
YGDKMEIPVDEYLIPIGKAQIVVPGADVTLIAHSRMVTICKEVVNELTKMGICAELIDLR
CCCCCCCCHHHHEECCCCEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHC
TVKPLDIATIANSVKKTNRCVIVEEGHLFAGIAAEVGFQIMEHCFDYLDAPLERVCQRET
CCCCCHHHHHHHHHHHCCCEEEEECCCEEEHHHHHHHHHHHHHHHHHHCHHHHHHHHCCC
PMPYSKVLEKETMPNKQRILSAIYKTLQKN
CCCHHHHHHHHCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10796014; 11481430 [H]