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The map label for this gene is dagA [C]

Identifier: 46447290

GI number: 46447290

Start: 1993208

End: 1994605

Strand: Reverse

Name: dagA [C]

Synonym: pc1656

Alternate gene names: 46447290

Gene position: 1994605-1993208 (Counterclockwise)

Preceding gene: 46447291

Following gene: 46447289

Centisome position: 82.61

GC content: 40.27

Gene sequence:

>1398_bases
ATGCCTGCTAATATAATGACAAGTATCATGAATAGTTTAGATATAATTTATAGCTGGATATGGGGGGCTCCTCTTCTCGT
GCTTTTAACTGGATTGGGAATTTATTTGACCATCACCTTACGAGGATTACAATTTCGTTATTTAGGTTATGCACTTCGAT
TAGTCTTTGGTTCTCAAAAAGCCCACCCAGAAGCAGAAGGACAAGGAGATATCAGCCACTTCCAATCGTTGATGACAGCC
TTAGCTGCTACAATTGGAATTGGAAATATTGCGGGTGTTGCAACAGCCATTACAGTCGGTGGACTAGGAGCTCTTTTTTG
GATGTGGGTGACTGCTTTATTGGGAATGGCGACGAAATATGCAGAAGCTATTTTAGCGGTAAAATATCGTGTTACTGACG
AGCATGGAGAAATGTGTGGTGGGCCCATGTATTTTATCGAATCGGGTTTAGGTTGGAAATGGTTGGCTTGTTCATTTGCT
TTTTTTGGAGCAGTCGCTGCTTTGGGTGGAGGAAACATGTTGCAAGCTAACTCTGTAGCAGATGTTATGCGTACGATGTT
TCATGTAAATCCTTGGTGGTCTGGTCTAATTATTGCAGCATTGACAGGCTTTACCATTGTGGGAGGAATTAAAAGTATTG
GAAAAGTCGCTGGTTTATTAGTTCCGTTCATGGCTCTTTTTTATATTGCGGGAGCAAGTATTATTTTGGCGTATCACTAC
GATCGTATCTTTGATGTCATTGTTGCTATCATTACCCATGCGTTTACGGGTCAAGCTGCATTTGGTGGTTTCTTAGGTTC
AACAATGCTATTAGCACTTCGAGTCGGGGTGAGTAGAGGATTAATGACAAGTGAGGCTGGACTAGGAACAGCATCAATTG
CCGCAGCTGCAGCAAAAACAGATCTTCCAGGTCGCCAAGCCTTAGTTTCCATGACAGGCTCTTTTTTAGCGACTATTATT
ATGTGCTCCGTGACAGGCCTTGTACTTGGCGTGACCGGTGTATTTGGTTCGCTAGATGAGCAAGGAAAATTGCTTAATGG
CGCTTCTATGACTGTTGCAGCCTTTAACTCTATTTTTACTGGTGGGGGGTATATTGTAACAATTGGATTAGTCCTTTTTG
CTTTTACAACGTTGCTTGGTTGGGCTTATTACGGTGAAAAATGTGTTGAATATCTTTTTGGTATTAAGTCTGTCCCCATT
TATCGAGTTTTATTTACTTTAATGATTATTCCAGGAGCTGTTTTAGAACTAGATATTGTTTGGAAAATTTCTGATGTTTT
CAACGGATTAATGGCTTTTCCAAATTTAATTGGATTGTGTGCACTTTCGAGTGTTGTCATTTCAGAAACAAAGTTATTTT
TGAATGTCTTAAAAGCTGAAAGAGAAGCTGAGAATTAG

Upstream 100 bases:

>100_bases
TTATCTAAAGTTATTAAAAAAGAAACCAAAGATTTTTTAATTGTGGTGGATCGTGAAAAAGAAAAAAGGGAAAATTCCAG
TGGTCTAGGAGATACATAAA

Downstream 100 bases:

>100_bases
GCTAAGCTTATTTCTAAATAAAAACCTAGTAGAAATTTCAGGTGAGTTAAAAAATAATTTGTTAGATGCCGCTTGGATGC
TTTGTTAATAAGTTTTTGAA

Product: putative D-alanine/glycine transport protein, sodium-dependent

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MPANIMTSIMNSLDIIYSWIWGAPLLVLLTGLGIYLTITLRGLQFRYLGYALRLVFGSQKAHPEAEGQGDISHFQSLMTA
LAATIGIGNIAGVATAITVGGLGALFWMWVTALLGMATKYAEAILAVKYRVTDEHGEMCGGPMYFIESGLGWKWLACSFA
FFGAVAALGGGNMLQANSVADVMRTMFHVNPWWSGLIIAALTGFTIVGGIKSIGKVAGLLVPFMALFYIAGASIILAYHY
DRIFDVIVAIITHAFTGQAAFGGFLGSTMLLALRVGVSRGLMTSEAGLGTASIAAAAAKTDLPGRQALVSMTGSFLATII
MCSVTGLVLGVTGVFGSLDEQGKLLNGASMTVAAFNSIFTGGGYIVTIGLVLFAFTTLLGWAYYGEKCVEYLFGIKSVPI
YRVLFTLMIIPGAVLELDIVWKISDVFNGLMAFPNLIGLCALSSVVISETKLFLNVLKAEREAEN

Sequences:

>Translated_465_residues
MPANIMTSIMNSLDIIYSWIWGAPLLVLLTGLGIYLTITLRGLQFRYLGYALRLVFGSQKAHPEAEGQGDISHFQSLMTA
LAATIGIGNIAGVATAITVGGLGALFWMWVTALLGMATKYAEAILAVKYRVTDEHGEMCGGPMYFIESGLGWKWLACSFA
FFGAVAALGGGNMLQANSVADVMRTMFHVNPWWSGLIIAALTGFTIVGGIKSIGKVAGLLVPFMALFYIAGASIILAYHY
DRIFDVIVAIITHAFTGQAAFGGFLGSTMLLALRVGVSRGLMTSEAGLGTASIAAAAAKTDLPGRQALVSMTGSFLATII
MCSVTGLVLGVTGVFGSLDEQGKLLNGASMTVAAFNSIFTGGGYIVTIGLVLFAFTTLLGWAYYGEKCVEYLFGIKSVPI
YRVLFTLMIIPGAVLELDIVWKISDVFNGLMAFPNLIGLCALSSVVISETKLFLNVLKAEREAEN
>Mature_464_residues
PANIMTSIMNSLDIIYSWIWGAPLLVLLTGLGIYLTITLRGLQFRYLGYALRLVFGSQKAHPEAEGQGDISHFQSLMTAL
AATIGIGNIAGVATAITVGGLGALFWMWVTALLGMATKYAEAILAVKYRVTDEHGEMCGGPMYFIESGLGWKWLACSFAF
FGAVAALGGGNMLQANSVADVMRTMFHVNPWWSGLIIAALTGFTIVGGIKSIGKVAGLLVPFMALFYIAGASIILAYHYD
RIFDVIVAIITHAFTGQAAFGGFLGSTMLLALRVGVSRGLMTSEAGLGTASIAAAAAKTDLPGRQALVSMTGSFLATIIM
CSVTGLVLGVTGVFGSLDEQGKLLNGASMTVAAFNSIFTGGGYIVTIGLVLFAFTTLLGWAYYGEKCVEYLFGIKSVPIY
RVLFTLMIIPGAVLELDIVWKISDVFNGLMAFPNLIGLCALSSVVISETKLFLNVLKAEREAEN

Specific function: Unknown

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family [H]

Homologues:

Organism=Escherichia coli, GI1786188, Length=454, Percent_Identity=32.5991189427313, Blast_Score=198, Evalue=6e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001463 [H]

Pfam domain/function: PF01235 Na_Ala_symp [H]

EC number: NA

Molecular weight: Translated: 49290; Mature: 49159

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: PS00873 NA_ALANINE_SYMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPANIMTSIMNSLDIIYSWIWGAPLLVLLTGLGIYLTITLRGLQFRYLGYALRLVFGSQK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEEEHHHHHHHHHHHHHHHHCCCC
AHPEAEGQGDISHFQSLMTALAATIGIGNIAGVATAITVGGLGALFWMWVTALLGMATKY
CCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEAILAVKYRVTDEHGEMCGGPMYFIESGLGWKWLACSFAFFGAVAALGGGNMLQANSVA
HHHHHHHHEEEECCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEECHHHHH
DVMRTMFHVNPWWSGLIIAALTGFTIVGGIKSIGKVAGLLVPFMALFYIAGASIILAYHY
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DRIFDVIVAIITHAFTGQAAFGGFLGSTMLLALRVGVSRGLMTSEAGLGTASIAAAAAKT
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHC
DLPGRQALVSMTGSFLATIIMCSVTGLVLGVTGVFGSLDEQGKLLNGASMTVAAFNSIFT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCHHHHHHHHHHHC
GGGYIVTIGLVLFAFTTLLGWAYYGEKCVEYLFGIKSVPIYRVLFTLMIIPGAVLELDIV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHEEEEHH
WKISDVFNGLMAFPNLIGLCALSSVVISETKLFLNVLKAEREAEN
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PANIMTSIMNSLDIIYSWIWGAPLLVLLTGLGIYLTITLRGLQFRYLGYALRLVFGSQK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEEEHHHHHHHHHHHHHHHHCCCC
AHPEAEGQGDISHFQSLMTALAATIGIGNIAGVATAITVGGLGALFWMWVTALLGMATKY
CCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEAILAVKYRVTDEHGEMCGGPMYFIESGLGWKWLACSFAFFGAVAALGGGNMLQANSVA
HHHHHHHHEEEECCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEECHHHHH
DVMRTMFHVNPWWSGLIIAALTGFTIVGGIKSIGKVAGLLVPFMALFYIAGASIILAYHY
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DRIFDVIVAIITHAFTGQAAFGGFLGSTMLLALRVGVSRGLMTSEAGLGTASIAAAAAKT
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHC
DLPGRQALVSMTGSFLATIIMCSVTGLVLGVTGVFGSLDEQGKLLNGASMTVAAFNSIFT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCHHHHHHHHHHHC
GGGYIVTIGLVLFAFTTLLGWAYYGEKCVEYLFGIKSVPIYRVLFTLMIIPGAVLELDIV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHEEEEHH
WKISDVFNGLMAFPNLIGLCALSSVVISETKLFLNVLKAEREAEN
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]