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The map label for this gene is dagA [C]

Identifier: 46447291

GI number: 46447291

Start: 1994607

End: 1995992

Strand: Reverse

Name: dagA [C]

Synonym: pc1657

Alternate gene names: 46447291

Gene position: 1995992-1994607 (Counterclockwise)

Preceding gene: 46447294

Following gene: 46447290

Centisome position: 82.67

GC content: 39.11

Gene sequence:

>1386_bases
GTGGATCAATTCTTAAGCGAACTTAGAAATTGGATTTGGGGATTGCCTTTGCTCATGCTATTGCTTGGCACTGGGGCTTA
TTTAACTATTCTTTTGAAAGGAGTGCAATTTCGTTATCTTGGGTATGCATTTAAGCAGGTCATTGCTAAGCAAAAACAAG
ACTCGCAAGGAGATATTAGCCATTTTCAAGCTTTAATGACCTCCTTAGCCGGGGCTATTGGAACAGGAACAATTGTTGGG
GTGGCGACAGCGATTACAATTGGAGGTTTAGGAGCTATTTTTTGGATGTGGGTAACAGCATTTTTAAGTATGGCGACTAA
ATATGCAGAGTCTTTGCTAGCCGTGAAATATCGTGAGCAAGATAGCCGAGGAGAAATGTCTGGGGGACCCATGCAATATA
TTGAAAAAGGTTTGGGATGGAAATGGATGGCATGGATTTTCGCTGTTTTAGGAATGATTGCAGCCATGAGTACGGGAAAT
CTTGTTCAAACAAATTCAATTGCTGAAGCTGTGAATCATGTATGGAATATTGAACCTTGGACTACAGGAGTTGTATTGTG
TTTTTTAACAGCAGCTGTGATCATTGGTGGAGTTAAAAGTATTGGGAATGTTGCAAGTGTTTTAGTCCCTGGAATGGCAA
TCATTTACATTTTAGCTGCTCTTTATATTCTAGTTGTTAATTATTCTTCAATTCCTCATGCATTTATGTTGATTTTTCAT
AGTGCTTTAAATGGTCAAGCTGCAGTTGGTGGCTTCCTCGGTTCTACAATGATATTGGCTTTACAGATGGGTGTAGCAAG
AAGTGTATTTTCTAATGAAGCAGGACTTGGAATATCTTCTATAGCTGCAGCAGCAGCCAAGACAGATAGTCCTGGACGTC
AGGCAATGGTGACAATGACTGGTGCATTAATTTCGACTGTCATCGTTTGTACGATGACAGGCCTTGTCCTCGCTGTATCA
AAACTAGTTGGTTCTTATTCACAGTCGGGTCAACTGTTAAATGGAGCTTCTCTAGCGATTGCTGCGTTTAATACTCACAT
TCAAGGTGGTGAATACCTTGTAACGATCGGTCTTGTTTTATTTGCGTATACCACGGTTCTTGCCTGGTCTTATTACGGAG
AAAAATGTTGTGAATATTTATTTGGGGAAAGTTCGGTTTTTAGCTATCGCATCTTATTCGCTTTAATGGTTATTCCGGGA
GCAGCATTAAAAATGGAGACTGCTTGGTTTTTAGCAGATATCAGTAACGGATTGATGGTGATTCCAAATTTAATTGCTTT
AATCGCTTTATCTAAAGTTATTAAAAAAGAAACCAAAGATTTTTTAATTGTGGTGGATCGTGAAAAAGAAAAAAGGGAAA
ATTCCAGTGGTCTAGGAGATACATAA

Upstream 100 bases:

>100_bases
TCTAACCAATTAGGTCTTACTTACAAAGACTAAATTATTTGTTAGTATCCTAACTAATTTACGATCGGGAACCTCATTTG
ATGGGTAAATAATGGAACAA

Downstream 100 bases:

>100_bases
AATGCCTGCTAATATAATGACAAGTATCATGAATAGTTTAGATATAATTTATAGCTGGATATGGGGGGCTCCTCTTCTCG
TGCTTTTAACTGGATTGGGA

Product: putative D-alanine/glycine transport protein, sodium-dependent

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MDQFLSELRNWIWGLPLLMLLLGTGAYLTILLKGVQFRYLGYAFKQVIAKQKQDSQGDISHFQALMTSLAGAIGTGTIVG
VATAITIGGLGAIFWMWVTAFLSMATKYAESLLAVKYREQDSRGEMSGGPMQYIEKGLGWKWMAWIFAVLGMIAAMSTGN
LVQTNSIAEAVNHVWNIEPWTTGVVLCFLTAAVIIGGVKSIGNVASVLVPGMAIIYILAALYILVVNYSSIPHAFMLIFH
SALNGQAAVGGFLGSTMILALQMGVARSVFSNEAGLGISSIAAAAAKTDSPGRQAMVTMTGALISTVIVCTMTGLVLAVS
KLVGSYSQSGQLLNGASLAIAAFNTHIQGGEYLVTIGLVLFAYTTVLAWSYYGEKCCEYLFGESSVFSYRILFALMVIPG
AALKMETAWFLADISNGLMVIPNLIALIALSKVIKKETKDFLIVVDREKEKRENSSGLGDT

Sequences:

>Translated_461_residues
MDQFLSELRNWIWGLPLLMLLLGTGAYLTILLKGVQFRYLGYAFKQVIAKQKQDSQGDISHFQALMTSLAGAIGTGTIVG
VATAITIGGLGAIFWMWVTAFLSMATKYAESLLAVKYREQDSRGEMSGGPMQYIEKGLGWKWMAWIFAVLGMIAAMSTGN
LVQTNSIAEAVNHVWNIEPWTTGVVLCFLTAAVIIGGVKSIGNVASVLVPGMAIIYILAALYILVVNYSSIPHAFMLIFH
SALNGQAAVGGFLGSTMILALQMGVARSVFSNEAGLGISSIAAAAAKTDSPGRQAMVTMTGALISTVIVCTMTGLVLAVS
KLVGSYSQSGQLLNGASLAIAAFNTHIQGGEYLVTIGLVLFAYTTVLAWSYYGEKCCEYLFGESSVFSYRILFALMVIPG
AALKMETAWFLADISNGLMVIPNLIALIALSKVIKKETKDFLIVVDREKEKRENSSGLGDT
>Mature_461_residues
MDQFLSELRNWIWGLPLLMLLLGTGAYLTILLKGVQFRYLGYAFKQVIAKQKQDSQGDISHFQALMTSLAGAIGTGTIVG
VATAITIGGLGAIFWMWVTAFLSMATKYAESLLAVKYREQDSRGEMSGGPMQYIEKGLGWKWMAWIFAVLGMIAAMSTGN
LVQTNSIAEAVNHVWNIEPWTTGVVLCFLTAAVIIGGVKSIGNVASVLVPGMAIIYILAALYILVVNYSSIPHAFMLIFH
SALNGQAAVGGFLGSTMILALQMGVARSVFSNEAGLGISSIAAAAAKTDSPGRQAMVTMTGALISTVIVCTMTGLVLAVS
KLVGSYSQSGQLLNGASLAIAAFNTHIQGGEYLVTIGLVLFAYTTVLAWSYYGEKCCEYLFGESSVFSYRILFALMVIPG
AALKMETAWFLADISNGLMVIPNLIALIALSKVIKKETKDFLIVVDREKEKRENSSGLGDT

Specific function: Unknown

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family [H]

Homologues:

Organism=Escherichia coli, GI1786188, Length=455, Percent_Identity=36.043956043956, Blast_Score=245, Evalue=3e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001463 [H]

Pfam domain/function: PF01235 Na_Ala_symp [H]

EC number: NA

Molecular weight: Translated: 49325; Mature: 49325

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHCCCCCCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]