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The map label for this gene is nrdA [H]

Identifier: 46446982

GI number: 46446982

Start: 1610455

End: 1612776

Strand: Reverse

Name: nrdA [H]

Synonym: pc1348

Alternate gene names: 46446982

Gene position: 1612776-1610455 (Counterclockwise)

Preceding gene: 46446983

Following gene: 46446981

Centisome position: 66.8

GC content: 36.99

Gene sequence:

>2322_bases
ATGTTTGTTACTAAACGAGACGGACGTAAACAAGAAGTTAAATTTGATAAAATTACAGCTCGCATTCTCAAGCTTTGCTA
TGGGCTAGATTCAGTGCATATCGATCCAGTTTTGATTGCTATGAAAGTGATAGAAGGGTTATTTGATGGAGTTACAACAA
GTGAATTAGACAATTTAGCTGCCGAAGTGGCAGCTTCATTAACTGTAAAGCATCCTGACTATGCTTTACTCGCTTCACGG
ATTGCTATTTCCAATTTACAAAAGAACACTAATAAATCATTTGCACGTACGATCGGTGAACTTTACCATTATATCGATCC
TAAAACAGGGAAAAAAGCCTCTTTAATTGCTGAGGATGTATACAAAATCGTGCAAAATCATGCCGAAATTTTAGATGCAA
CAATGATTTATGATCGTGATTTTGGTTACGACTATTTTGGTTTTAAGACGCTAGAGCGTTCTTATTTATTGAAAATTAAT
GGGAAAGTCATCGAAAGGCCTCAACATCTGCTCATGAGAGTATCCATTGGAATTCATAAAAATGATATTTCTGCAGCCAT
TGAAACCTATCATTGGATGAGTGAGCGTTGGTTTACCCATGCTACACCTACTTTATTTAATGCCGGAACACCTTATCCAC
AAATGTCTTCTTGTTTTTTGATGCAAGTAAAAGAAGATAGTATCGAAGGAATTTTTGACACGCTGAAAGCATGTGCAAAA
ATTTCTCAAGCTGCAGGAGGAATCGGTTTAAGTATTCATAATATCCGTGCGACAGGTTCTTATATTCGAGGAACGGGAGG
AATTTCTAATGGAATTATTCCTATGCTCCGCGTATATAATGATACGGCGCGCTATATTGATCAAGGAGGAGGAAAACGGA
AAGGATCGTTTGCCATTTATCTTGAACCTTGGCATGCCGATATCTACGCTTTTTTAGATTTAAGAAAGAATCACGGAAAA
GAAGAATTACGAGCACGCGATCTTTTTACAGCTCTTTGGATTCCAGACCTTTTTATGAAGCGGGTGGAAGAAGATGGCGT
TTGGTCGCTTTTTTGTCCTAATGAAGCACCCAATCTTCATGAATGTTGGGGAGAAGAATTTGAACATCTTTATTGTCTTT
ATGAACAAGAAGGAAAAGCTCGAGAGGTAATTAAAGCACAAGATTTATGGTTTAAAATTTTGGAATCTCAAATTGAAACA
GGAAACCCTTACATGCTTTTTAAGGATGCCTGTAATTATAAATCTAATCAAAAGCATTTAGGAACAATTAAGTTAAGTAA
TTTGTGTACAGAAATTATAGAGTACACTTCACCCCATGAAGCGGCTGTTTGTAATTTAGCCTCCATTGCTTTACCACGGT
TCATTATTAACGGGCATTTTGATCATCAAAAACTTTTTGAGGTTACGCAAGTGATCACAAGAAATTTAGATAAAATTATT
GATGAAAATAGTTATCCTATTGTGGAAGCGCAAACTTCAAACTTGCTTCATAGACCGATAGGGATTGGAGTACAAGGGCT
TGCAGATGCTTTCATCTTGCTGAGGATGCCTTTTGATTCAAAAGAAGCGCGAGCGTTAAATCAAGCCATTTTTGAAACTA
TTTACTTTGGAGCTTTGACAGCCTCTAAAGATTTAGCTAAAAAAAAAGGAAGATATCCTAGCTATGAAGGGTCACCGATT
TCTAAGGGAATTTTACAATATGAAATGTGGAATGTCAAACCTAGCTCAAATTGGGATTGGGAAGCATTAAAAACTGAAAT
AGCTGTCCACGGAATACGCAATTCTTTACTTGTGGCGCTCATGCCGACTGCCTCGACTTCACAAATTTTAGGAAATAATG
AGTGTTTTGAGCCTTATACCTCAAACTTATATACACGGCGTGTTTTATCGGGAGAATTTATTGTTGTGAACAAGCATTTG
ATGCGGGATCTTGTGAAAAGAGAGTTGTGGAACGAAGAGATGAAACAACGTATTTTAGCTGCTAACGGGTCTATTCAAGG
GATCGAAGAAATTCCATCTGAAATCAAAGAGCTATACAAAACTGTTTGGGAAATTAAGCAAAAAGCTTTGATAGATATGG
CTGCTGATAGAGGACCTTTTATTTGCCAGTCTCAATCGTTAAACTTATTTGTCGAAAGCCCAACTTTTGCAAAGCTCTCT
TCTATGCATTTTTATGCCTGGAAAAAAGGATTAAAAACAGGCATGTATTATTTGAGAATTAAAGCTGCTGCAGAAGCGAT
AAAATTTACCGTTAATCAAAACTATGAGCAGCCGCAACAAGAAATTGCTTGTTCTTTGGATTGCCTCAGCTGTAGTGGCT
AA

Upstream 100 bases:

>100_bases
TTTTCGAAAGGCGGGTATCAGAATATCAAAAATCAGGAGTGATGTCAACGAAAGAAAATCATGTATTTACCATTCACGAA
GATTTTTAAGGGAGTAAATC

Downstream 100 bases:

>100_bases
CGTTTGTCTCATATGGCTAAACACTGTCATTTTAAGGGGAAATAGCTAATTAATCAGCTACTTTCCCTTAAAATGACAAG
CGTTTCAACTTAATCCTAAT

Product: ribonucleoside-diphosphate reductase large chain

Products: NA

Alternate protein names: Ribonucleotide reductase [H]

Number of amino acids: Translated: 773; Mature: 773

Protein sequence:

>773_residues
MFVTKRDGRKQEVKFDKITARILKLCYGLDSVHIDPVLIAMKVIEGLFDGVTTSELDNLAAEVAASLTVKHPDYALLASR
IAISNLQKNTNKSFARTIGELYHYIDPKTGKKASLIAEDVYKIVQNHAEILDATMIYDRDFGYDYFGFKTLERSYLLKIN
GKVIERPQHLLMRVSIGIHKNDISAAIETYHWMSERWFTHATPTLFNAGTPYPQMSSCFLMQVKEDSIEGIFDTLKACAK
ISQAAGGIGLSIHNIRATGSYIRGTGGISNGIIPMLRVYNDTARYIDQGGGKRKGSFAIYLEPWHADIYAFLDLRKNHGK
EELRARDLFTALWIPDLFMKRVEEDGVWSLFCPNEAPNLHECWGEEFEHLYCLYEQEGKAREVIKAQDLWFKILESQIET
GNPYMLFKDACNYKSNQKHLGTIKLSNLCTEIIEYTSPHEAAVCNLASIALPRFIINGHFDHQKLFEVTQVITRNLDKII
DENSYPIVEAQTSNLLHRPIGIGVQGLADAFILLRMPFDSKEARALNQAIFETIYFGALTASKDLAKKKGRYPSYEGSPI
SKGILQYEMWNVKPSSNWDWEALKTEIAVHGIRNSLLVALMPTASTSQILGNNECFEPYTSNLYTRRVLSGEFIVVNKHL
MRDLVKRELWNEEMKQRILAANGSIQGIEEIPSEIKELYKTVWEIKQKALIDMAADRGPFICQSQSLNLFVESPTFAKLS
SMHFYAWKKGLKTGMYYLRIKAAAEAIKFTVNQNYEQPQQEIACSLDCLSCSG

Sequences:

>Translated_773_residues
MFVTKRDGRKQEVKFDKITARILKLCYGLDSVHIDPVLIAMKVIEGLFDGVTTSELDNLAAEVAASLTVKHPDYALLASR
IAISNLQKNTNKSFARTIGELYHYIDPKTGKKASLIAEDVYKIVQNHAEILDATMIYDRDFGYDYFGFKTLERSYLLKIN
GKVIERPQHLLMRVSIGIHKNDISAAIETYHWMSERWFTHATPTLFNAGTPYPQMSSCFLMQVKEDSIEGIFDTLKACAK
ISQAAGGIGLSIHNIRATGSYIRGTGGISNGIIPMLRVYNDTARYIDQGGGKRKGSFAIYLEPWHADIYAFLDLRKNHGK
EELRARDLFTALWIPDLFMKRVEEDGVWSLFCPNEAPNLHECWGEEFEHLYCLYEQEGKAREVIKAQDLWFKILESQIET
GNPYMLFKDACNYKSNQKHLGTIKLSNLCTEIIEYTSPHEAAVCNLASIALPRFIINGHFDHQKLFEVTQVITRNLDKII
DENSYPIVEAQTSNLLHRPIGIGVQGLADAFILLRMPFDSKEARALNQAIFETIYFGALTASKDLAKKKGRYPSYEGSPI
SKGILQYEMWNVKPSSNWDWEALKTEIAVHGIRNSLLVALMPTASTSQILGNNECFEPYTSNLYTRRVLSGEFIVVNKHL
MRDLVKRELWNEEMKQRILAANGSIQGIEEIPSEIKELYKTVWEIKQKALIDMAADRGPFICQSQSLNLFVESPTFAKLS
SMHFYAWKKGLKTGMYYLRIKAAAEAIKFTVNQNYEQPQQEIACSLDCLSCSG
>Mature_773_residues
MFVTKRDGRKQEVKFDKITARILKLCYGLDSVHIDPVLIAMKVIEGLFDGVTTSELDNLAAEVAASLTVKHPDYALLASR
IAISNLQKNTNKSFARTIGELYHYIDPKTGKKASLIAEDVYKIVQNHAEILDATMIYDRDFGYDYFGFKTLERSYLLKIN
GKVIERPQHLLMRVSIGIHKNDISAAIETYHWMSERWFTHATPTLFNAGTPYPQMSSCFLMQVKEDSIEGIFDTLKACAK
ISQAAGGIGLSIHNIRATGSYIRGTGGISNGIIPMLRVYNDTARYIDQGGGKRKGSFAIYLEPWHADIYAFLDLRKNHGK
EELRARDLFTALWIPDLFMKRVEEDGVWSLFCPNEAPNLHECWGEEFEHLYCLYEQEGKAREVIKAQDLWFKILESQIET
GNPYMLFKDACNYKSNQKHLGTIKLSNLCTEIIEYTSPHEAAVCNLASIALPRFIINGHFDHQKLFEVTQVITRNLDKII
DENSYPIVEAQTSNLLHRPIGIGVQGLADAFILLRMPFDSKEARALNQAIFETIYFGALTASKDLAKKKGRYPSYEGSPI
SKGILQYEMWNVKPSSNWDWEALKTEIAVHGIRNSLLVALMPTASTSQILGNNECFEPYTSNLYTRRVLSGEFIVVNKHL
MRDLVKRELWNEEMKQRILAANGSIQGIEEIPSEIKELYKTVWEIKQKALIDMAADRGPFICQSQSLNLFVESPTFAKLS
SMHFYAWKKGLKTGMYYLRIKAAAEAIKFTVNQNYEQPQQEIACSLDCLSCSG

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 ATP-cone domains [H]

Homologues:

Organism=Homo sapiens, GI4506749, Length=754, Percent_Identity=67.5066312997347, Blast_Score=1071, Evalue=0.0,
Organism=Escherichia coli, GI2367133, Length=647, Percent_Identity=29.8299845440495, Blast_Score=249, Evalue=5e-67,
Organism=Escherichia coli, GI1789030, Length=635, Percent_Identity=25.6692913385827, Blast_Score=181, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI17555242, Length=781, Percent_Identity=64.0204865556978, Blast_Score=1057, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320914, Length=759, Percent_Identity=64.163372859025, Blast_Score=1068, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6322123, Length=759, Percent_Identity=62.3188405797101, Blast_Score=1051, Evalue=0.0,
Organism=Drosophila melanogaster, GI17352481, Length=799, Percent_Identity=61.5769712140175, Blast_Score=1055, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005144
- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926 [H]

Pfam domain/function: PF03477 ATP-cone; PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 87727; Mature: 87727

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: PS51161 ATP_CONE ; PS00089 RIBORED_LARGE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFVTKRDGRKQEVKFDKITARILKLCYGLDSVHIDPVLIAMKVIEGLFDGVTTSELDNLA
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHH
AEVAASLTVKHPDYALLASRIAISNLQKNTNKSFARTIGELYHYIDPKTGKKASLIAEDV
HHHHHHEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
YKIVQNHAEILDATMIYDRDFGYDYFGFKTLERSYLLKINGKVIERPQHLLMRVSIGIHK
HHHHHHHHHHHHHHHEEECCCCCCHHHHHHHCCEEEEEECCHHHHCHHHHHHHHHCCCCC
NDISAAIETYHWMSERWFTHATPTLFNAGTPYPQMSSCFLMQVKEDSIEGIFDTLKACAK
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHH
ISQAAGGIGLSIHNIRATGSYIRGTGGISNGIIPMLRVYNDTARYIDQGGGKRKGSFAIY
HHHHCCCCCEEEEEEEECCCEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEE
LEPWHADIYAFLDLRKNHGKEELRARDLFTALWIPDLFMKRVEEDGVWSLFCPNEAPNLH
EECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHH
ECWGEEFEHLYCLYEQEGKAREVIKAQDLWFKILESQIETGNPYMLFKDACNYKSNQKHL
HHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCEE
GTIKLSNLCTEIIEYTSPHEAAVCNLASIALPRFIINGHFDHQKLFEVTQVITRNLDKII
CEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
DENSYPIVEAQTSNLLHRPIGIGVQGLADAFILLRMPFDSKEARALNQAIFETIYFGALT
CCCCCCEEEECCCCHHHCCCCCCHHHHHCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
ASKDLAKKKGRYPSYEGSPISKGILQYEMWNVKPSSNWDWEALKTEIAVHGIRNSLLVAL
HHHHHHHHCCCCCCCCCCCHHHHHHEEEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEE
MPTASTSQILGNNECFEPYTSNLYTRRVLSGEFIVVNKHLMRDLVKRELWNEEMKQRILA
ECCCCCHHHCCCCCCCCCHHCCHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHHH
ANGSIQGIEEIPSEIKELYKTVWEIKQKALIDMAADRGPFICQSQSLNLFVESPTFAKLS
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEEECCCHHHHH
SMHFYAWKKGLKTGMYYLRIKAAAEAIKFTVNQNYEQPQQEIACSLDCLSCSG
HHHHHHHHHHHHCCEEEEEEHHHHHHHEEEECCCCCCHHHHHHHHHHEECCCC
>Mature Secondary Structure
MFVTKRDGRKQEVKFDKITARILKLCYGLDSVHIDPVLIAMKVIEGLFDGVTTSELDNLA
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHH
AEVAASLTVKHPDYALLASRIAISNLQKNTNKSFARTIGELYHYIDPKTGKKASLIAEDV
HHHHHHEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
YKIVQNHAEILDATMIYDRDFGYDYFGFKTLERSYLLKINGKVIERPQHLLMRVSIGIHK
HHHHHHHHHHHHHHHEEECCCCCCHHHHHHHCCEEEEEECCHHHHCHHHHHHHHHCCCCC
NDISAAIETYHWMSERWFTHATPTLFNAGTPYPQMSSCFLMQVKEDSIEGIFDTLKACAK
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHH
ISQAAGGIGLSIHNIRATGSYIRGTGGISNGIIPMLRVYNDTARYIDQGGGKRKGSFAIY
HHHHCCCCCEEEEEEEECCCEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEE
LEPWHADIYAFLDLRKNHGKEELRARDLFTALWIPDLFMKRVEEDGVWSLFCPNEAPNLH
EECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHH
ECWGEEFEHLYCLYEQEGKAREVIKAQDLWFKILESQIETGNPYMLFKDACNYKSNQKHL
HHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCEE
GTIKLSNLCTEIIEYTSPHEAAVCNLASIALPRFIINGHFDHQKLFEVTQVITRNLDKII
CEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
DENSYPIVEAQTSNLLHRPIGIGVQGLADAFILLRMPFDSKEARALNQAIFETIYFGALT
CCCCCCEEEECCCCHHHCCCCCCHHHHHCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
ASKDLAKKKGRYPSYEGSPISKGILQYEMWNVKPSSNWDWEALKTEIAVHGIRNSLLVAL
HHHHHHHHCCCCCCCCCCCHHHHHHEEEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEE
MPTASTSQILGNNECFEPYTSNLYTRRVLSGEFIVVNKHLMRDLVKRELWNEEMKQRILA
ECCCCCHHHCCCCCCCCCHHCCHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHHH
ANGSIQGIEEIPSEIKELYKTVWEIKQKALIDMAADRGPFICQSQSLNLFVESPTFAKLS
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEEECCCHHHHH
SMHFYAWKKGLKTGMYYLRIKAAAEAIKFTVNQNYEQPQQEIACSLDCLSCSG
HHHHHHHHHHHHCCEEEEEEHHHHHHHEEEECCCCCCHHHHHHHHHHEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10684935 [H]