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The map label for this gene is murF [H]

Identifier: 46446887

GI number: 46446887

Start: 1494503

End: 1495840

Strand: Reverse

Name: murF [H]

Synonym: pc1253

Alternate gene names: 46446887

Gene position: 1495840-1494503 (Counterclockwise)

Preceding gene: 46446888

Following gene: 46446886

Centisome position: 61.95

GC content: 36.7

Gene sequence:

>1338_bases
ATGAGAATAATGACTTTAAAACAGATTGCTAAAATCTTGAATTCCTCTTTGCCTGAAGTAGAGGGTGTAGTTAAAGGATT
TTGTGTAGATAGTCGGCTAACAAAAAAAAATGAGGTTTTTGTCGCTTTAAGGGGAGAGCATGTAGATGGGCACGCCTATT
TGAATGAAGTGAAGCAAAAAGGAGCCTTAGCGGCAATCGTCGATCAAAATTATCAAGGGCCTGTATTTGGGATGCATTTA
TTTCGAACAGAAAATCCTTTACTAGCTCTCCAAATGCTAGCTCAAAAATGTCTTGAAAAAACGAGCCGTGTTGTAGCTGT
AACAGGTTCAATAGGGAAAACAACCACAAAAGAGTTTATTAAAACACTTTTAGAGCAGAGATATGTCACTGCTGCTTCCC
AGGGAAATAGCAATTCACAGGTAGGTCTTCCTTTAACTGTATTAAATCATACGACTGGGACGGAAGATATCTTAGTTTTA
GAAATGGGAATGACAGAACCTGGGCAGCTGAGTACATTAGTCAATATTGCTCATCCAGAAGTGTCTGTATTGACAACAGC
TGCTTTAGTCCATGCTTGTCGCTTTGAAACATTAAAAGATATCGCTTTAACAAAAGCAGAAATATTCTCTCATTCGCATA
CTCGATTAGGTGTGGTTGATCGAGAGATAGAAGCTTATGAAACAATTGCTCAAATAGGAGAATGCAAAAAAATATCCTTT
TCGACAGTAAGCTCTAGGGCTGATTATCATTTAGAAACTGTTAATGAAGAAGTTAGATTAAAAACTGAAGGACAGAGTAT
TTCTCTTGGTAAAATGTGTTTGCCAGGTAAACACAATCTTCACAACTTACTTGCTGCGATTGCTGTTGCGAGGTATTTCG
AGCTAACTTGGAATGAAATTAAACAAGCTATCCCTTTTCTTAAATTACCCGAGAGGCGCCTACAATTCGTTCAAAAAGAG
GGTGTTTATTTTTTAAATGATTCTTATAATGCTTCGGAATTATCGGTCAAATCTGCCTTAGAAACGCTGCCAAATGCAAC
GGGAAAAGGTTATAAAATTGCTGTTTTAGGTAGTATGATGGAGCTTGGACATTTTTCAGATGAATGTCACCAACGTGTTG
GTGAGTTTGCTTTAAATTATGTGGATAAAATGTACTGTCTTGGTGCTGAGTGTTTACCTATTGTTGAGGTTTGGAAAAAA
GCAAATCGACCTGTTGAATTATTTATGGAACGTTCTGAGCTAATTTCAACTTTAAAAAAAAACTTAAAACCCGCCGATGT
TGTTTTATTGAAAGGATCTAGATCCAAAGAGTTGTGGAAAATATTAGAAGAACTGTAA

Upstream 100 bases:

>100_bases
AATCTGCGATTTCTTATCCAATCCATGCTTTATTTGGGATAAGAGAAGAAGCTAATAAAGATAAAGCCGGACTAAGGTTT
TTTAAATAGGATTAGCATCA

Downstream 100 bases:

>100_bases
AAAATAACTAAAGAGCTTTATTTATGATTTTGTTTGCAATTGATTACTTGGAAAGGGTTTGGACTCTAAAAGTTCCAAGC
GTCTTTAGTTATTATTCTAC

Product: putative UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate- D-alanyl-D-alanine-ligase murF

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MRIMTLKQIAKILNSSLPEVEGVVKGFCVDSRLTKKNEVFVALRGEHVDGHAYLNEVKQKGALAAIVDQNYQGPVFGMHL
FRTENPLLALQMLAQKCLEKTSRVVAVTGSIGKTTTKEFIKTLLEQRYVTAASQGNSNSQVGLPLTVLNHTTGTEDILVL
EMGMTEPGQLSTLVNIAHPEVSVLTTAALVHACRFETLKDIALTKAEIFSHSHTRLGVVDREIEAYETIAQIGECKKISF
STVSSRADYHLETVNEEVRLKTEGQSISLGKMCLPGKHNLHNLLAAIAVARYFELTWNEIKQAIPFLKLPERRLQFVQKE
GVYFLNDSYNASELSVKSALETLPNATGKGYKIAVLGSMMELGHFSDECHQRVGEFALNYVDKMYCLGAECLPIVEVWKK
ANRPVELFMERSELISTLKKNLKPADVVLLKGSRSKELWKILEEL

Sequences:

>Translated_445_residues
MRIMTLKQIAKILNSSLPEVEGVVKGFCVDSRLTKKNEVFVALRGEHVDGHAYLNEVKQKGALAAIVDQNYQGPVFGMHL
FRTENPLLALQMLAQKCLEKTSRVVAVTGSIGKTTTKEFIKTLLEQRYVTAASQGNSNSQVGLPLTVLNHTTGTEDILVL
EMGMTEPGQLSTLVNIAHPEVSVLTTAALVHACRFETLKDIALTKAEIFSHSHTRLGVVDREIEAYETIAQIGECKKISF
STVSSRADYHLETVNEEVRLKTEGQSISLGKMCLPGKHNLHNLLAAIAVARYFELTWNEIKQAIPFLKLPERRLQFVQKE
GVYFLNDSYNASELSVKSALETLPNATGKGYKIAVLGSMMELGHFSDECHQRVGEFALNYVDKMYCLGAECLPIVEVWKK
ANRPVELFMERSELISTLKKNLKPADVVLLKGSRSKELWKILEEL
>Mature_445_residues
MRIMTLKQIAKILNSSLPEVEGVVKGFCVDSRLTKKNEVFVALRGEHVDGHAYLNEVKQKGALAAIVDQNYQGPVFGMHL
FRTENPLLALQMLAQKCLEKTSRVVAVTGSIGKTTTKEFIKTLLEQRYVTAASQGNSNSQVGLPLTVLNHTTGTEDILVL
EMGMTEPGQLSTLVNIAHPEVSVLTTAALVHACRFETLKDIALTKAEIFSHSHTRLGVVDREIEAYETIAQIGECKKISF
STVSSRADYHLETVNEEVRLKTEGQSISLGKMCLPGKHNLHNLLAAIAVARYFELTWNEIKQAIPFLKLPERRLQFVQKE
GVYFLNDSYNASELSVKSALETLPNATGKGYKIAVLGSMMELGHFSDECHQRVGEFALNYVDKMYCLGAECLPIVEVWKK
ANRPVELFMERSELISTLKKNLKPADVVLLKGSRSKELWKILEEL

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=459, Percent_Identity=30.5010893246187, Blast_Score=160, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 49561; Mature: 49561

Theoretical pI: Translated: 7.82; Mature: 7.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIMTLKQIAKILNSSLPEVEGVVKGFCVDSRLTKKNEVFVALRGEHVDGHAYLNEVKQK
CCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHC
GALAAIVDQNYQGPVFGMHLFRTENPLLALQMLAQKCLEKTSRVVAVTGSIGKTTTKEFI
CCEEEEECCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHH
KTLLEQRYVTAASQGNSNSQVGLPLTVLNHTTGTEDILVLEMGMTEPGQLSTLVNIAHPE
HHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCEEEEEECCCCCCCHHHHEEECCCC
VSVLTTAALVHACRFETLKDIALTKAEIFSHSHTRLGVVDREIEAYETIAQIGECKKISF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHHCCCCCCCH
STVSSRADYHLETVNEEVRLKTEGQSISLGKMCLPGKHNLHNLLAAIAVARYFELTWNEI
HHHHCCCCHHHHHCCCCEEEEECCCEEECCCEECCCCCCHHHHHHHHHHHHHHHHHHHHH
KQAIPFLKLPERRLQFVQKEGVYFLNDSYNASELSVKSALETLPNATGKGYKIAVLGSMM
HHHCCCCCCCHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEEEEHHHH
ELGHFSDECHQRVGEFALNYVDKMYCLGAECLPIVEVWKKANRPVELFMERSELISTLKK
HHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
NLKPADVVLLKGSRSKELWKILEEL
CCCCCEEEEECCCCCHHHHHHHHHC
>Mature Secondary Structure
MRIMTLKQIAKILNSSLPEVEGVVKGFCVDSRLTKKNEVFVALRGEHVDGHAYLNEVKQK
CCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHC
GALAAIVDQNYQGPVFGMHLFRTENPLLALQMLAQKCLEKTSRVVAVTGSIGKTTTKEFI
CCEEEEECCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHH
KTLLEQRYVTAASQGNSNSQVGLPLTVLNHTTGTEDILVLEMGMTEPGQLSTLVNIAHPE
HHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCEEEEEECCCCCCCHHHHEEECCCC
VSVLTTAALVHACRFETLKDIALTKAEIFSHSHTRLGVVDREIEAYETIAQIGECKKISF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHHCCCCCCCH
STVSSRADYHLETVNEEVRLKTEGQSISLGKMCLPGKHNLHNLLAAIAVARYFELTWNEI
HHHHCCCCHHHHHCCCCEEEEECCCEEECCCEECCCCCCHHHHHHHHHHHHHHHHHHHHH
KQAIPFLKLPERRLQFVQKEGVYFLNDSYNASELSVKSALETLPNATGKGYKIAVLGSMM
HHHCCCCCCCHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEEEEHHHH
ELGHFSDECHQRVGEFALNYVDKMYCLGAECLPIVEVWKKANRPVELFMERSELISTLKK
HHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
NLKPADVVLLKGSRSKELWKILEEL
CCCCCEEEEECCCCCHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]