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The map label for this gene is engD [H]

Identifier: 46446386

GI number: 46446386

Start: 930869

End: 931966

Strand: Direct

Name: engD [H]

Synonym: pc0752

Alternate gene names: 46446386

Gene position: 930869-931966 (Clockwise)

Preceding gene: 46446385

Following gene: 46446387

Centisome position: 38.55

GC content: 38.34

Gene sequence:

>1098_bases
ATGTCAACAAACCTCTCTGTAGGAATTGTTGGTTTACCCAATGTCGGTAAATCAACGCTTTTCAATGCCCTTACATCTAA
TCAAGCAGCTGCTTCTAACTATCCTTTCTGTACGATTGATCCAAATATTGGAATTGTTGATGTGGTGGATGATCGCTTAG
ATCGTTTGTCTGCTTTGTCCAATAGTAAGAAAATTGTCTACGCGAATATGCAATTTGTCGATATTGCGGGAATTGTTGAA
GGGGCATCTAAGGGAGAAGGATTAGGAAATAAATTTTTATCAAACATTCGGGAAACAGATGCAATTGTTCATGTCGTACG
TTGCTTTGAATCAAGTGATATTATCCATGTCGCGGGTAAAGTTGATCCAATTCATGACATTGAAATTATTAATATGGAAC
TGCGTTTAGCAGATTTACAAATGATTGAAAATGTACTGGCCCGTTTAGAAAAACAAGCCAAAACAAAAAAAGAACTCGTG
CTCAGTTTGGAAACATTAAAAAAAGTTTCCGAACATTTAAATGAGAATAAACCCGTTAGAACTCTTGAATTAACAGACGA
GGAAAAAGAAAGTATTGAGGCTTATCGTTTTCTTTCTTCTAAAAAAGTTTTATATGTGGCCAATGTTTCTGAAGAAGAAT
TACCAGAAATGGATAATGAATTTGTTCGCCGAGTTAAAGCTTATGCAGAAGCAGAGGGAAATGCTGTTATTCCGATTTGT
GCTAAATTAGAAGAAGAAATTGCTCAGCTCCCTTTGGCTGAAAGGAAAAGTTTCTTAGAAAGCTTGGGCCTTCAGCAATC
AGGCCTTGAGCGTTTGATCAAAGCTTCTTTTAATATGCTTGGACTGTTGACCTATTTAACAACAGGTGAAATTGAGACGC
GAGCTTGGACTATCACCGAGGGAACTCATGCGGCTGAAGCAGCAGGCAAAATTCATTCGGACATTCAAAAAGGATTTATT
CGTGCAGAAGTGGTTGCCTTTGATGATATGATGACTTACAAAGGCCGAGCTGGAGCAAGGGAGCAAGGAAAGGTTCGGGC
TGAAGGAAGAGACTACCTTGTCAAAGATGGGGATGTTATCCTTTTTATGCATCATTAA

Upstream 100 bases:

>100_bases
GTCATGTTGCAAGAAATGGGAGTTCTTTTAGACAGCGCTGTTGCTCCAATTTCAATATTTTGATATTTTAAACCTCACTT
TTGTTTAAAAGGATTTAATT

Downstream 100 bases:

>100_bases
TCTTAATCATTCTAAAGGAACTATTAAGTGAACGGAGAAAAAAAATTATTATTTGTCAGCTGTGCCTCTGCGCTGGAACC
TCTTTTGTTAGAAGAACTTC

Product: GTP-dependent nucleic acid-binding protein EngD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 365; Mature: 364

Protein sequence:

>365_residues
MSTNLSVGIVGLPNVGKSTLFNALTSNQAAASNYPFCTIDPNIGIVDVVDDRLDRLSALSNSKKIVYANMQFVDIAGIVE
GASKGEGLGNKFLSNIRETDAIVHVVRCFESSDIIHVAGKVDPIHDIEIINMELRLADLQMIENVLARLEKQAKTKKELV
LSLETLKKVSEHLNENKPVRTLELTDEEKESIEAYRFLSSKKVLYVANVSEEELPEMDNEFVRRVKAYAEAEGNAVIPIC
AKLEEEIAQLPLAERKSFLESLGLQQSGLERLIKASFNMLGLLTYLTTGEIETRAWTITEGTHAAEAAGKIHSDIQKGFI
RAEVVAFDDMMTYKGRAGAREQGKVRAEGRDYLVKDGDVILFMHH

Sequences:

>Translated_365_residues
MSTNLSVGIVGLPNVGKSTLFNALTSNQAAASNYPFCTIDPNIGIVDVVDDRLDRLSALSNSKKIVYANMQFVDIAGIVE
GASKGEGLGNKFLSNIRETDAIVHVVRCFESSDIIHVAGKVDPIHDIEIINMELRLADLQMIENVLARLEKQAKTKKELV
LSLETLKKVSEHLNENKPVRTLELTDEEKESIEAYRFLSSKKVLYVANVSEEELPEMDNEFVRRVKAYAEAEGNAVIPIC
AKLEEEIAQLPLAERKSFLESLGLQQSGLERLIKASFNMLGLLTYLTTGEIETRAWTITEGTHAAEAAGKIHSDIQKGFI
RAEVVAFDDMMTYKGRAGAREQGKVRAEGRDYLVKDGDVILFMHH
>Mature_364_residues
STNLSVGIVGLPNVGKSTLFNALTSNQAAASNYPFCTIDPNIGIVDVVDDRLDRLSALSNSKKIVYANMQFVDIAGIVEG
ASKGEGLGNKFLSNIRETDAIVHVVRCFESSDIIHVAGKVDPIHDIEIINMELRLADLQMIENVLARLEKQAKTKKELVL
SLETLKKVSEHLNENKPVRTLELTDEEKESIEAYRFLSSKKVLYVANVSEEELPEMDNEFVRRVKAYAEAEGNAVIPICA
KLEEEIAQLPLAERKSFLESLGLQQSGLERLIKASFNMLGLLTYLTTGEIETRAWTITEGTHAAEAAGKIHSDIQKGFIR
AEVVAFDDMMTYKGRAGAREQGKVRAEGRDYLVKDGDVILFMHH

Specific function: GTP-dependent nucleic acid-binding protein which may act as a translation factor [H]

COG id: COG0012

COG function: function code J; Predicted GTPase, probable translation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI58761500, Length=368, Percent_Identity=44.5652173913043, Blast_Score=306, Evalue=2e-83,
Organism=Homo sapiens, GI58761502, Length=228, Percent_Identity=37.719298245614, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI24308117, Length=214, Percent_Identity=27.5700934579439, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787454, Length=362, Percent_Identity=51.6574585635359, Blast_Score=385, Evalue=1e-108,
Organism=Escherichia coli, GI1789574, Length=128, Percent_Identity=35.15625, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17509631, Length=369, Percent_Identity=42.2764227642276, Blast_Score=299, Evalue=2e-81,
Organism=Caenorhabditis elegans, GI17552324, Length=193, Percent_Identity=26.9430051813472, Blast_Score=64, Evalue=1e-10,
Organism=Saccharomyces cerevisiae, GI6319499, Length=372, Percent_Identity=38.7096774193548, Blast_Score=249, Evalue=6e-67,
Organism=Saccharomyces cerevisiae, GI6321773, Length=384, Percent_Identity=34.1145833333333, Blast_Score=219, Evalue=6e-58,
Organism=Saccharomyces cerevisiae, GI6321649, Length=354, Percent_Identity=27.4011299435028, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24640873, Length=372, Percent_Identity=41.9354838709677, Blast_Score=285, Evalue=3e-77,
Organism=Drosophila melanogaster, GI24640877, Length=372, Percent_Identity=41.9354838709677, Blast_Score=285, Evalue=3e-77,
Organism=Drosophila melanogaster, GI24640875, Length=372, Percent_Identity=41.9354838709677, Blast_Score=285, Evalue=3e-77,
Organism=Drosophila melanogaster, GI24640879, Length=327, Percent_Identity=39.1437308868502, Blast_Score=222, Evalue=3e-58,
Organism=Drosophila melanogaster, GI24585318, Length=137, Percent_Identity=32.1167883211679, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR004396
- InterPro:   IPR013029
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR012676
- InterPro:   IPR023192 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF06071 YchF-GTPase_C [H]

EC number: NA

Molecular weight: Translated: 40437; Mature: 40306

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTNLSVGIVGLPNVGKSTLFNALTSNQAAASNYPFCTIDPNIGIVDVVDDRLDRLSALS
CCCCCEEEEEECCCCCHHHHHHHHHCCCCCCCCCCEEEECCCCCEEEHHHHHHHHHHHHC
NSKKIVYANMQFVDIAGIVEGASKGEGLGNKFLSNIRETDAIVHVVRCFESSDIIHVAGK
CCCEEEEECCEEEEHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
VDPIHDIEIINMELRLADLQMIENVLARLEKQAKTKKELVLSLETLKKVSEHLNENKPVR
CCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
TLELTDEEKESIEAYRFLSSKKVLYVANVSEEELPEMDNEFVRRVKAYAEAEGNAVIPIC
EEEECCCHHHHHHHHHHHCCCCEEEEECCCHHHCCCHHHHHHHHHHHHHHCCCCEEEHHH
AKLEEEIAQLPLAERKSFLESLGLQQSGLERLIKASFNMLGLLTYLTTGEIETRAWTITE
HHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEC
GTHAAEAAGKIHSDIQKGFIRAEVVAFDDMMTYKGRAGAREQGKVRAEGRDYLVKDGDVI
CCHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHCCCCCCCCCCCCEEECCCCEEEECCCEE
LFMHH
EEEEC
>Mature Secondary Structure 
STNLSVGIVGLPNVGKSTLFNALTSNQAAASNYPFCTIDPNIGIVDVVDDRLDRLSALS
CCCCEEEEEECCCCCHHHHHHHHHCCCCCCCCCCEEEECCCCCEEEHHHHHHHHHHHHC
NSKKIVYANMQFVDIAGIVEGASKGEGLGNKFLSNIRETDAIVHVVRCFESSDIIHVAGK
CCCEEEEECCEEEEHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
VDPIHDIEIINMELRLADLQMIENVLARLEKQAKTKKELVLSLETLKKVSEHLNENKPVR
CCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
TLELTDEEKESIEAYRFLSSKKVLYVANVSEEELPEMDNEFVRRVKAYAEAEGNAVIPIC
EEEECCCHHHHHHHHHHHCCCCEEEEECCCHHHCCCHHHHHHHHHHHHHHCCCCEEEHHH
AKLEEEIAQLPLAERKSFLESLGLQQSGLERLIKASFNMLGLLTYLTTGEIETRAWTITE
HHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEC
GTHAAEAAGKIHSDIQKGFIRAEVVAFDDMMTYKGRAGAREQGKVRAEGRDYLVKDGDVI
CCHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHCCCCCCCCCCCCEEECCCCEEEECCCEE
LFMHH
EEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]