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The map label for this gene is gcpE

Identifier: 46446374

GI number: 46446374

Start: 911982

End: 913946

Strand: Direct

Name: gcpE

Synonym: pc0740

Alternate gene names: 46446374

Gene position: 911982-913946 (Clockwise)

Preceding gene: 46446371

Following gene: 46446377

Centisome position: 37.77

GC content: 39.69

Gene sequence:

>1965_bases
ATGGTCCAAAAAAAATATTGTGAGGCGATTCATCAAACAGAGAGGCGCCCGACGCGCATTGTTAATGTGGGAAATGTTGG
CATTGGAGGCAACCATCCTATTCGTATCCAATCCATGACAACATCCAGTACACGAGATGTGGAAGCGACAATTGAACAAG
TCATCCGTTTGGCTGATCAGGGATGCGAAATTGTTCGCGTGACAGTCCAGGGAATTAAAGAAGCGGATGCGTGTGAACAT
ATCAAAAAGGGGTTGATTAAACGTGGTTATCAAATTCCTCTTGTGGCGGATATCCATTTTTATCCTCCTGCAGCCATGCG
TGTTGTGGATTTTGTAGACAAAGTACGCATTAATCCGGGAAATTTTGTCGATAAACGTGCGAGCTTTAAGCAAATTGTTT
ATGATGATGAGTCTTATGCCAGAGAAATTGAGAGGATTGAAGAAAAATTTACTCCCTTAGTGGAAAAGTGTAAACGGTTA
AACCGTGCTATGAGAATTGGAACCAATCATGGCTCATTATCCGATCGAATCATGAACCGATATGGAGATACTCCTTTTGG
AATGGTCGAGTCAGCTCTGGAATTTGCTCGCATTTGTCGAAAAAATGATTATCATAACTTTCTTTTTTCTATGAAAGCTT
CGAATCCACAAGTCATGATTCAAGCTTATCGTTTATTGACGCAAGCCATGTATGCATTGGAGTGGGATTATCCCTTACAC
TTAGGTGTGACAGAAGCCGGAGAAGGGGAAGATGGGCGAATCAAATCTGCGATGGGAATTGGATCTCTTTTGATTGATGG
AATTGGGGATACCATTCGTGTTTCGTTAACAGAAGATCCTTGGCACGAGATAAATCCTTGCCAACGATTGATTAAACTGG
CTTCTGCTTATCAACAGCAAGGTGTGGCTCCTTTTATAGAAAATTATCGTCAGATAGAAGCAATCGAACGACGTCAAGTG
CACTTATCTTCAACCGTTCCCATGCATCGTGACGGAACAGTTTTTATTTCGTTACCGATTAATATGCTTAAAGAGGCTTC
TCTCTATCAACAAATTGGTTGTGAAGGCCCTTTTGGGAAACCTAAATTAAAGACTGCCACAGCAGATAATTTAGTTTTAA
AAAATCCAAATTCTGACTCTGAAGAAAAACGGCAGCTTCAAATTTTAAAAGATTTAGGAATTGGCCTTTTTTCAAAAGAT
CCTTTTGAAATGAGTTTGGTTATTCACCCGTTAAAAAAATGGCTCCAGTCCCGTGCAGTGGATTCTTTTGCTTCCCGTTT
TTCTTCATCATGGGCTAAATCCGCTGGGCAGCCCTTGATCATTCAAATAACTGATGAAACTGAAAAAGAGTGGAAAGAAG
TTATTTCTTTAAAACCACAATTAATTATTTTATCTCCTTCAACTAATCGCTTACACTATTCAAGACAATTTTTTGAGTGG
CTACAGCAAAATCAATTAAATTATCCAGTCATTTTAAACTTCACTTATCAAGGAGAAAATGAAGATACCATTCTTTTAGC
TAGTATGGAATGTGGATCCCTTTTATGTGATGGACTTGGAGAAGGAGTTTGGTTAGAAGGTCCTTATGATATTCTTTTTC
TTCGTCAATTGAGTTTTTCCATTTTACAGGCGGCTCGCCTTCGCATGTCTAAAACTGATTTTATTTCTTGCCCAAGCTGT
GGACGGACATTATTTAATCTTCAAGATGTCACTAAACGAATTCAGTCTCGTACCTCTCATTTACCGGGCGTTAAAATTGC
GATTATGGGATGCATTGTTAACGGGCCGGGGGAAATGGCCGATGCAGATTTTGGGTACGTTGGATCTAAGCCTGGTAAAA
TCGATCTTTACGTTGGGAAAGAATGCGTGGAAAAAGATATCGATTTTGCAGATGCAGATGATCGTCTTGTCAATTTAATC
AGAGCACATGGTCGTTGGATAGAACCTCAAACGGTCAATGCATAA

Upstream 100 bases:

>100_bases
AAAGATTCCCTTGGGATAAAGTCATTGGCATTTTGGCTAACTTCTGTTATATTTTTTTTCTTAGAGAACGAAAAGTTTTA
AACTTGAAAAGGTGTTCGTC

Downstream 100 bases:

>100_bases
CATTCGCCATCGATGTTAATTTAACGATGAGCATTTATGGGGTTCCCAGGCAGTTTCAGGCACATTTAAGTGAAAATTGA
CAAATCTTGACACAACAAAT

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase

Number of amino acids: Translated: 654; Mature: 654

Protein sequence:

>654_residues
MVQKKYCEAIHQTERRPTRIVNVGNVGIGGNHPIRIQSMTTSSTRDVEATIEQVIRLADQGCEIVRVTVQGIKEADACEH
IKKGLIKRGYQIPLVADIHFYPPAAMRVVDFVDKVRINPGNFVDKRASFKQIVYDDESYAREIERIEEKFTPLVEKCKRL
NRAMRIGTNHGSLSDRIMNRYGDTPFGMVESALEFARICRKNDYHNFLFSMKASNPQVMIQAYRLLTQAMYALEWDYPLH
LGVTEAGEGEDGRIKSAMGIGSLLIDGIGDTIRVSLTEDPWHEINPCQRLIKLASAYQQQGVAPFIENYRQIEAIERRQV
HLSSTVPMHRDGTVFISLPINMLKEASLYQQIGCEGPFGKPKLKTATADNLVLKNPNSDSEEKRQLQILKDLGIGLFSKD
PFEMSLVIHPLKKWLQSRAVDSFASRFSSSWAKSAGQPLIIQITDETEKEWKEVISLKPQLIILSPSTNRLHYSRQFFEW
LQQNQLNYPVILNFTYQGENEDTILLASMECGSLLCDGLGEGVWLEGPYDILFLRQLSFSILQAARLRMSKTDFISCPSC
GRTLFNLQDVTKRIQSRTSHLPGVKIAIMGCIVNGPGEMADADFGYVGSKPGKIDLYVGKECVEKDIDFADADDRLVNLI
RAHGRWIEPQTVNA

Sequences:

>Translated_654_residues
MVQKKYCEAIHQTERRPTRIVNVGNVGIGGNHPIRIQSMTTSSTRDVEATIEQVIRLADQGCEIVRVTVQGIKEADACEH
IKKGLIKRGYQIPLVADIHFYPPAAMRVVDFVDKVRINPGNFVDKRASFKQIVYDDESYAREIERIEEKFTPLVEKCKRL
NRAMRIGTNHGSLSDRIMNRYGDTPFGMVESALEFARICRKNDYHNFLFSMKASNPQVMIQAYRLLTQAMYALEWDYPLH
LGVTEAGEGEDGRIKSAMGIGSLLIDGIGDTIRVSLTEDPWHEINPCQRLIKLASAYQQQGVAPFIENYRQIEAIERRQV
HLSSTVPMHRDGTVFISLPINMLKEASLYQQIGCEGPFGKPKLKTATADNLVLKNPNSDSEEKRQLQILKDLGIGLFSKD
PFEMSLVIHPLKKWLQSRAVDSFASRFSSSWAKSAGQPLIIQITDETEKEWKEVISLKPQLIILSPSTNRLHYSRQFFEW
LQQNQLNYPVILNFTYQGENEDTILLASMECGSLLCDGLGEGVWLEGPYDILFLRQLSFSILQAARLRMSKTDFISCPSC
GRTLFNLQDVTKRIQSRTSHLPGVKIAIMGCIVNGPGEMADADFGYVGSKPGKIDLYVGKECVEKDIDFADADDRLVNLI
RAHGRWIEPQTVNA
>Mature_654_residues
MVQKKYCEAIHQTERRPTRIVNVGNVGIGGNHPIRIQSMTTSSTRDVEATIEQVIRLADQGCEIVRVTVQGIKEADACEH
IKKGLIKRGYQIPLVADIHFYPPAAMRVVDFVDKVRINPGNFVDKRASFKQIVYDDESYAREIERIEEKFTPLVEKCKRL
NRAMRIGTNHGSLSDRIMNRYGDTPFGMVESALEFARICRKNDYHNFLFSMKASNPQVMIQAYRLLTQAMYALEWDYPLH
LGVTEAGEGEDGRIKSAMGIGSLLIDGIGDTIRVSLTEDPWHEINPCQRLIKLASAYQQQGVAPFIENYRQIEAIERRQV
HLSSTVPMHRDGTVFISLPINMLKEASLYQQIGCEGPFGKPKLKTATADNLVLKNPNSDSEEKRQLQILKDLGIGLFSKD
PFEMSLVIHPLKKWLQSRAVDSFASRFSSSWAKSAGQPLIIQITDETEKEWKEVISLKPQLIILSPSTNRLHYSRQFFEW
LQQNQLNYPVILNFTYQGENEDTILLASMECGSLLCDGLGEGVWLEGPYDILFLRQLSFSILQAARLRMSKTDFISCPSC
GRTLFNLQDVTKRIQSRTSHLPGVKIAIMGCIVNGPGEMADADFGYVGSKPGKIDLYVGKECVEKDIDFADADDRLVNLI
RAHGRWIEPQTVNA

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family

Homologues:

Organism=Escherichia coli, GI1788863, Length=273, Percent_Identity=41.025641025641, Blast_Score=177, Evalue=2e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPG_PARUW (Q6MD85)

Other databases:

- EMBL:   BX908798
- RefSeq:   YP_007739.1
- ProteinModelPortal:   Q6MD85
- STRING:   Q6MD85
- GeneID:   2780947
- GenomeReviews:   BX908798_GR
- KEGG:   pcu:pc0740
- NMPDR:   fig|264201.1.peg.740
- eggNOG:   COG0821
- HOGENOM:   HBG335271
- OMA:   SMRIGTN
- PhylomeDB:   Q6MD85
- BioCyc:   CPRO264201:PC0740-MONOMER
- BioCyc:   PCHL-E25-01:PCHL-E25-01-000740-MONOMER
- HAMAP:   MF_00159
- InterPro:   IPR017178
- InterPro:   IPR004588
- PIRSF:   PIRSF037336
- TIGRFAMs:   TIGR00612

Pfam domain/function: PF04551 GcpE

EC number: =1.17.7.1

Molecular weight: Translated: 73896; Mature: 73896

Theoretical pI: Translated: 7.32; Mature: 7.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQKKYCEAIHQTERRPTRIVNVGNVGIGGNHPIRIQSMTTSSTRDVEATIEQVIRLADQ
CCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHC
GCEIVRVTVQGIKEADACEHIKKGLIKRGYQIPLVADIHFYPPAAMRVVDFVDKVRINPG
CCCEEEEEHHHCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCHHHHHHHHHHHHHCCCCC
NFVDKRASFKQIVYDDESYAREIERIEEKFTPLVEKCKRLNRAMRIGTNHGSLSDRIMNR
CCHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
YGDTPFGMVESALEFARICRKNDYHNFLFSMKASNPQVMIQAYRLLTQAMYALEWDYPLH
HCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCEE
LGVTEAGEGEDGRIKSAMGIGSLLIDGIGDTIRVSLTEDPWHEINPCQRLIKLASAYQQQ
ECCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHC
GVAPFIENYRQIEAIERRQVHLSSTVPMHRDGTVFISLPINMLKEASLYQQIGCEGPFGK
CCCHHHHHHHHHHHHHHHHEEHHCCCCCCCCCEEEEEECHHHHHHHHHHHHHCCCCCCCC
PKLKTATADNLVLKNPNSDSEEKRQLQILKDLGIGLFSKDPFEMSLVIHPLKKWLQSRAV
CCCEEECCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
DSFASRFSSSWAKSAGQPLIIQITDETEKEWKEVISLKPQLIILSPSTNRLHYSRQFFEW
HHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHH
LQQNQLNYPVILNFTYQGENEDTILLASMECGSLLCDGLGEGVWLEGPYDILFLRQLSFS
HHHCCCCCCEEEEEEECCCCCCEEEEEECCHHHHHHHHCCCCEEECCCHHHHHHHHHHHH
ILQAARLRMSKTDFISCPSCGRTLFNLQDVTKRIQSRTSHLPGVKIAIMGCIVNGPGEMA
HHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCC
DADFGYVGSKPGKIDLYVGKECVEKDIDFADADDRLVNLIRAHGRWIEPQTVNA
CCCCCCCCCCCCEEEEEECHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MVQKKYCEAIHQTERRPTRIVNVGNVGIGGNHPIRIQSMTTSSTRDVEATIEQVIRLADQ
CCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHC
GCEIVRVTVQGIKEADACEHIKKGLIKRGYQIPLVADIHFYPPAAMRVVDFVDKVRINPG
CCCEEEEEHHHCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCHHHHHHHHHHHHHCCCCC
NFVDKRASFKQIVYDDESYAREIERIEEKFTPLVEKCKRLNRAMRIGTNHGSLSDRIMNR
CCHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
YGDTPFGMVESALEFARICRKNDYHNFLFSMKASNPQVMIQAYRLLTQAMYALEWDYPLH
HCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCEE
LGVTEAGEGEDGRIKSAMGIGSLLIDGIGDTIRVSLTEDPWHEINPCQRLIKLASAYQQQ
ECCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHC
GVAPFIENYRQIEAIERRQVHLSSTVPMHRDGTVFISLPINMLKEASLYQQIGCEGPFGK
CCCHHHHHHHHHHHHHHHHEEHHCCCCCCCCCEEEEEECHHHHHHHHHHHHHCCCCCCCC
PKLKTATADNLVLKNPNSDSEEKRQLQILKDLGIGLFSKDPFEMSLVIHPLKKWLQSRAV
CCCEEECCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
DSFASRFSSSWAKSAGQPLIIQITDETEKEWKEVISLKPQLIILSPSTNRLHYSRQFFEW
HHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHH
LQQNQLNYPVILNFTYQGENEDTILLASMECGSLLCDGLGEGVWLEGPYDILFLRQLSFS
HHHCCCCCCEEEEEEECCCCCCEEEEEECCHHHHHHHHCCCCEEECCCHHHHHHHHHHHH
ILQAARLRMSKTDFISCPSCGRTLFNLQDVTKRIQSRTSHLPGVKIAIMGCIVNGPGEMA
HHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCC
DADFGYVGSKPGKIDLYVGKECVEKDIDFADADDRLVNLIRAHGRWIEPQTVNA
CCCCCCCCCCCCEEEEEECHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA